4. Bisphosphonates promoting repair of injured vertebrae after thoracolumbar fracture internal fixation: A randomized controlled study
Academic Journal of Second Military Medical University 2017;38(4):443-446
Objective To explore the anti-osteoporosis effect of bisphosphonates on repairing injured vertebrae after thoracolumbar fracture internal fixation through a randomized controlled study. Methods Eighty-four patients with thoracolumbar fracture treated by orthopaedic internal fixation in Department of Orthopaedics, Nanjing General Hospital from Jun. 2014 to Jun. 2015 were included, and the patients were divided into the bisphosphonate treatment group (n=42) and control group (n=42) by random number method. The patients in both groups were given the routine anti-osteoporosis drugs such as calcitriol and calcium carbonate D3 after surgery; in addition, the patients in the bisphosphonate treatment group were also given alendronate sodium D3 tablets (each containing alendronate sodium 70 mg, 1 tablet per week), while the control group received a placebo. The bone mineral density (BMD) in thoracolumbar vertebral injury area of patients in the two groups was measured and compared at 1 month, 3 months, 6 months and 1 year after surgery. Results The BMD values of patients in two groups were significantly decreased immediately after reset compared with preoperation, and then they were increased continuously in follow-up. There was no significant difference in BMD between the two groups at 1 month or 3 months after sursery (P>0.05), while the BMD in the bisphosphonate treatment group was significantly higher than that in the control group at 6 months and 1 year after surgery (P<0.05). Conclusion Bisphosphaonate drugs can accelerate the repair of vertebral osteoporosis after thoracolumbar fracture internal fixation, showing a good clinical application value.
5.Correlation between Angiotensin Converting Enzyme Gene Polymorphism and Kawasaki Disease
dong-hai, LIU ; xiu-ying, WANG ; yi, XU
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To investigate the correlation between angiotensin converting enzyme(ACE) gene polymorphism and kawasaki disease(KD).Methods A 287 bp Alu fragment in intron 16 of the ACE gene was used as insertion(I)/deletion(D) polymorphism marker. The ACE genotype of 28 children (10 children complicated coronary dilataltion) with KD and 35 healthy controls were detected by polymerase chain reaction (PCR), and ACE concentration in blood serum was measured by ultraviolet-spectrophotometer assay.Results 1.The ACE concentration was significantly higher in KD group than that in healthy control group(P
6.Hypertrophic cardiomyophthy: a family report.
Hai-Yun DONG ; Xiu-Ying WANG ; Yi XU
Chinese Journal of Contemporary Pediatrics 2010;12(6):1 p folowing 512-1 p folowing 512
Adolescent
;
Adult
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Cardiomyopathy, Hypertrophic
;
genetics
;
Child
;
Humans
;
Male
7.L1-L2 complete traumatic fracture-dislocation of the lumbar spine: a case report.
Gang-xiang WANG ; Zhi-gang WANG ; Hai-dong ZHOU ; Hong-yu XU
China Journal of Orthopaedics and Traumatology 2015;28(9):868-869
Adult
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Humans
;
Joint Dislocations
;
surgery
;
Lumbar Vertebrae
;
injuries
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surgery
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Male
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Spinal Fractures
;
surgery
8.HER2 testing in breast cancer: rereading the ASCO/CAP guideline.
Wei WANG ; Jing-jing XIANG ; Hai-dong CUI ; Jian LIU ; Ru-jun XU
Chinese Journal of Surgery 2013;51(10):875-878
9. Surgical treatment for thoracolumbar fractures of the spine
Journal of Medical Postgraduates 2019;32(2):113-118
With the development of transportation and industry,car accidents,falling accidents and firearm injuries have be⁃come the main causes of spinal fractures and spinal cord injuries. The thoracolumbar spine is the joint of thoracic kyphosis and lumbarlordosis after human bipedalism. From the perspective of biomechanics,it belongs to the weakest area of the spine and is prone to trau⁃matic fractures. A review from the literatures suggests that thoracolumbar fractures account for 40% of all spinal fractures. In recent years,with the development of biomechanics,biomaterials and digital medicine of the spine,new ideas,techniques and materials have been used for surgical treatment of spinal fractures.“Reconstruction stabilization and early rehabilitation”has become the princi⁃ple of treatment for spine surgeons to treat spinal thoracolumbar fractures. Regardless of patients with severe spinal cord injury or ad⁃vanced osteoporosis,perioperative evaluation,preoperative planning,and intraoperative injury control operation should be made,and surgical treatment should be the first choice for early rehabilitation and social return. New technologies and viewpoints such as digitalspine surgery,biomaterials,biomechanics and spinal surgery robots are gradually applied in the clinic. While applying these technolo⁃ gies,there are also many hot issues that deserve our attention. Not only the surgeries for thoracolumbar fractures,but also the surgical indications and expected effects have been rapidly developed and improved.
10.Expression and location of ARMS2 protein in normal eye tissue
Zhong-fang, ZHAO ; Hai-feng, XU ; Xiao-guang, DONG ; Ting, LIU
Chinese Journal of Experimental Ophthalmology 2011;29(11):994-997
Background Researches determined that the alteration of A69S locus of age-related maculopathy susceptibility 2 ( ARMS2 ) gene is closely associated with the pathogenesis and progression of age-related maculopathy ( AM D ).However,the location of ARMS2 protein in normal eye tissue is still in controversy,therefore,its function is below understanding up to now.Objective The goal of this laboratory work was to investigate the distribution,expression and location of ARMS2 protein in normal adult retina and choroid as well as in retinal pigment epithelial (RPE) cells and lay a basis for exploring further its function in the protein level.Methods Ten donor eyeballs of normal adult male with the age from 28-42 years were collected in eye bank of Qingdao Eye Hospital.The frozen sections of the retina and choroid were prepared for the detection and location of ARMS2 in 3 eyes by immunofluorescence under the confocal laser microscope.The retina was isolated for the primary culture of RPE cells using explant culture method.The cells were then identified by CK32 antibody by immunofluorescence.The distribution and expression of the ARMS2 protein in retina,ehoroid and RPE cells were determined by immunofluorescence technique.Results ARMS2 protein was strongly expressed in retinal vessel,RPE cell layer,Bruch membrane and choroidal vessel,but weak expression was in retinal ganglion cell layer,inner nuclear layer,outer plexiform layer,outer nuclear layer and inner plexiform layer in the normal eyes.The primarily cultured cells appeared the polygon shape with the abundant pigment in cytoplasm.The immunofluorescence of the cells showed the positive response for CK32,exhibiting the green fluorescence granules in the cytoplasm.The positive expression of ARMS2 protein also was seen in the cytoplasm of RPE cells,appearing the red fluorescence.Conclusions ARMS2 protein mainly distribute and locate retinal and choroidal vessels,RPE cells and Bruch membrane in normal eye.