1.Measurement of intraocular stray light and its clinical significance
Chinese Journal of Experimental Ophthalmology 2012;(12):1139-1143
Stray light is a physical phenomenon that degrades visual quality when light scattering occurs in the human eye.It is a main cause of the disabling glare.Stray light originates in cornea,iris,sclera,lens and retina,and these ocular tissues contribute to the total amount of stray light in a normal eye.The light scattering intensity increases when the transparency of any optical element mentioned above reduces.Stray light can be measured by several different methods,but the measurements still lacks a standardized procedure and outcome.Further clinical study is necessary to quantify and standardize the measuring method of stray light.This article aims to introduce the concept,source and clinical significance of stray light,and to provide its advanced measurement techniques and methods.
2.Carbamazepine caused severe drug eruption in treatment of tinnitus.
Hai-bo YANG ; Ding-qiang HUANG ; Yu-bo ZHU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(3):248-249
Adult
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Carbamazepine
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adverse effects
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therapeutic use
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Drug Eruptions
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etiology
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Female
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Humans
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Tinnitus
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drug therapy
3.Posterior indirect reduction and internal fixation and laminectomy in the treatment of thoracolumbar vertebrae burst fracture complicated with spinal cord injury
Chen HUANG ; Qionghua FENG ; Bo CAO ; Wei LIN ; Hai XU
Journal of Regional Anatomy and Operative Surgery 2017;26(3):204-208
Objective To explore the clinical effect of posterior indirect reduction and internal fixation and laminectomy in the treatment of thoracolumbar vertebrae burst fracture complicated with spinal cord injury.Methods Eighty patients with thoracolumbar vertebrae burst fracture and spinal cord injury treated in our hospital from March 2014 to March 2015 were selected as the objects,and they were divided into reset group and laminectomy group with forty cases in each group according to surgical method.All the patients were followed up for 1 year,the lumbar function of two groups at 1 week and 1 year after operation were observed respectively,and the pain degree was observed in 1month,3 months and 6 months after operation.The amount of bleeding,operation time,hospitalization time and fracture healing time were observed.Neurological function was assessed by classification criteria of the American Spinal Cord Injury Association(ASIA),and incidence of complications was figured in the two groups.Results The anterior heights of the injured vertebra were higher than those before the operation,and the Cobb's angles were lower than those before the operation,the differences were significant(P < 0.05);while there was no significant differences in the anterior heights of the injured vertebra between the two groups at 1 week and 1 year after operation(P > 0.05).VAS scores of the two groups after 1 month,3 months and 6 months decreased significantly when compared with the preoperative scores(P < 0.05),and VAS scores of each time in the reset group were significantly lower than those in the laminectomy group(P < 0.05).The amount of bleeding,operation time,hospitalization time and fracture healing time in the reset group were less than those in the laminectomy group (P < 0.05).The neurological function recovery of the two groups were significantly improved when compared with that before the operation(P <0.05).There was no significant difference in recovery of neurological function between the two groups(P > 0.05).The complication rate was 7.50% in the reset group,lower than 12.50% of the laminectomy group,the difference was significant (P < 0.05).Conclusion Posterior indirect reduction and internal fixation of lamina both have a certain effect in the treatment of thoracolumbar vertebrae burst fracture complicated with spinal cord injury.But posterior indirect reduction has less complications and less amount of bleeding,which is beneficial to postoperative recovery.
4.Biological characteristics of nucleus pulposus cells from human degenerative intervertebral disc using the improved simple method
Hai WANG ; Bo HUANG ; Yue ZHOU ; Jian WANG ; Changqing LI
Chinese Journal of Tissue Engineering Research 2013;(28):5139-5144
BACKGROUND: The commonly used culture methods for primary intervertebral disc cells are type Ⅱcol agenase alone digestion method, and type Ⅱ col agenase combined trypin digestion method. However, the acquired cells are few. OBJECTIVE: To acquire nucleus pulposus cells from human degenerative intervertebral disc using a systemic and simplified method. METHODS: Nucleus pulposus cells were isolated and cultured. The morphology of nucleus pulposus cells was observed under inverted phase contrast microscope every day. Primary and subcultured cellsuspension was applied for the determination of cel viability using trypan blue staining. The cel growth was detected with MTT assay. The cel morphology was observed under laser confocal microscopy. RESULTS AND CONCLUSION: Nucleus pulposus cells were successful y isolated from from human degenerative intervertebral disc using the improved method, and cells were subcultured to passage 3. Primary cells were fusiform shaped, while cells at passage 1 and 2 were triangle or polygonal, which were similar to fibroblasts. When cells almost reached the confluence, the cells showed slabstone-like appearance. Trypan blue staining showed that, the primary cel viability was 99%, and passage 3 cells had 93%-95% viability. The proliferation of nucleus pulposus cells gradual y decreased as the generation increased. Compared with passage 1 cells, passage 2 and 3 cells at logarithmic phase trended to be smoother. The cel morphology observed under laser confocal microscopy was similar to the results under phase contrast microscope. The improved simple method can successful y acquire a variety of cells from human degenerative intervertebral disc, and these cells show fine biological properties.
5.Report of a case of sphenoid sinus meningoencephaloceles misdiagnosed as cysts.
Yan-hong XU ; Ding-qing HUANG ; Hai-bo YANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(5):430-430
Diagnostic Errors
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Encephalocele
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diagnosis
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Female
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Humans
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Meningocele
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diagnosis
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Middle Aged
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Mucocele
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diagnosis
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Sphenoid Sinus
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pathology
6.Effect of Different Types of Tube Feeding on Swallowing Function for Stroke Patients
Shao-chun HUANG ; Hai-yan QIU ; Wei-bo SHAO
Chinese Journal of Rehabilitation Theory and Practice 2012;18(12):1104-1106
Objective To evaluate the effect of different types of tube feeding on swallowing function of stroke patients. Methods 60 patients with dysphagia post stroke were divided into observation group and control group with 30 cases in each group. The control group received indwelling nasogastric tube feeding, and the observation group received intermittent nasogastric tube feeding. They were assessed with Kubota Water Swallow Test (WST) and VGF on the 1st day and 30th day after admission. Results The scores of drinking test and VGF were better in the observation group than in the control group (P<0.05) on the 30th day. Conclusion Long-term nasogatric tube feeding is effective on dysphagia in stroke patients.
7.Tension reduced incision through anterior tibial approach combined with locking compression plate fixation for treatment of elderly patients with distal tibiofibular fractures.
Hai HUANG ; Xi-Cai ZHANG ; Bo-Wei SHI ; Hua PAN ; Li-Jiang XU ; Hai-Qiang ZUO
China Journal of Orthopaedics and Traumatology 2014;27(6):453-457
OBJECTIVETo explore effective approaches of treating elderly patients with distal tibiofibular fractures.
METHODSFrom August 2008 to October 2012,175 elderly patients with distal tibiofibular fractures were treated with locking compression plate (LCP) through anterior tibial. There were 112 males and 63 females with an average of 71.3 (ranged 60 to 83) years old. Of them,89 cases were treated by anterior tibial tension reduced incision with LCP,including 62 males and 27 females with a mean age of (71.8 +/- 6.4) years old. Eighty-six patients were treated by distal tibial incision with LCP,including 58 males and 28 females with a mean age of (70.3 +/- 6.7) years old. Swelling time, operation time, intraoperative blood loss, hospital stay, healing time, complications and AOFAS scores were compared between two groups after operation.
RESULTSSwelling time in anterior tension reduced incision with LCP and distal tibial incision with LCP was (5.6 +/- 1.3) and (9.7 +/- 2.1) days, healing time was (4.2 +/- 1.4) and (5.4 +/- 1.9) months,and complications were found 3 in tension reduced incision and 10 in distak tibial incision respectively;and all data shown statistically significant differences between two groups (P < 0.05). At 12 months after operation,AOFAS score was 89.0 +/- 9.7, 87.9 +/- 9.4; and there was no statistically significant difference between two groups (P > 0.05).
CONCLUSIONTension reduced incision through anterior tibial combined with locking compression plate fixation in treating elderly patients with distal tibiofibular fractures can provide good clinical effects with quick fracture healing and low complications.
Adult ; Aged ; Aged, 80 and over ; Bone Plates ; Female ; Fibula ; injuries ; surgery ; Fracture Fixation, Internal ; instrumentation ; methods ; Humans ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Tibia ; injuries ; surgery ; Tibial Fractures ; surgery ; Treatment Outcome
8.The effective parts of liangxue tongyu prescription on cooling-blood and activating-blood and analysis of chemical constituents by HPLC-MS and GC-MS.
Xi HUANG ; Guo-chun LI ; Lian YIN ; Zi-han ZHANG ; Yi-xin LIANG ; Hai-bo CHEN
Acta Pharmaceutica Sinica 2015;50(1):86-93
In order to clarify material basis of effective parts of liangxue tongyu prescription, blood-heat and blood-stasis rat model induced by dry yeast was established. The changes of rectal temperature, blood viscosity and plasma viscosity were used to evaluate the cooling-blood and activating-blood effects of liangxue tongyu prescription and its parts. Compared with the model group, the extract from liangxue tongyu prescription, its volatile oil and n-butanol part could significantly reduce rectal temperature (P<0.01), and also reduce blood viscosity and plasma viscosity to various degrees (P<0.01 or P<0.05). So volatile oil and n-butanol part were primarily identified as effective parts of liangxue tongyu prescription. By using GC-MS with normalization method of area to analyze volatile oil of liangxue tongyu prescription, 70 compounds were identified, accounting for about 92.54%, mainly as β-asarone, paeonol, α-asarone and shyobunone. 42 compounds such as peony glycosides, tannins, and iridoid glycosides were identified by HPLC-MS techniques and standard comparison. The study determined the effective parts of liangxue tongyu prescription and clarified the chemical composition providing the foundation for further studies on material basis of liangxue tongyu prescription.
Acetophenones
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chemistry
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Animals
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Anisoles
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chemistry
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Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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chemistry
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Gas Chromatography-Mass Spectrometry
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Oils, Volatile
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chemistry
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Rats
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Tannins
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chemistry
9.Influence of Excessive Na Stress on Growth and Element Accumulation of Three Species of Ectomycorrhizal Fungi
Yi HUANG ; Xue-Yan JIANG ; Hai-Bo JI ; Tian-Ping XU ;
Microbiology 1992;0(03):-
This study deals with colony expansion characteristics, biomass increment and Na accumulation in mycelia of three ectomycorrhizal fungi ( Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus ) under treatment of Na2SO4 and NaCl. The experimental results showed that no impact was observed from Na2SO4 and NaCl treatments on the growth mode of the mycelia, but the biomass increment of X. chrysenteron and G. viscidus was significantly restrained under 0.1 mol/L Nacl. Under the treatment of Na2SO4, biomass increment of X. chrysenteron is significantly higher than the control, but the biomass of G. viscidus is lower than that of the control, and no significant impact was measured on growth of B. edulis under both treatments of Na2 SO4 and NaCl. The experiment also indicated that Na accumulation in mycelia varied significantly among the three tested strains, highest Na accumulation was measured in X. chrysenteron under treatment of NaCl, while under the treatment of Na2SO4 , accumulation of Na in B. edulis is much higher than the other two strains.
10.Clinical evaluation of naloxone used in the postoperation of cerebral tumor
Zhen-Hua YU ; Hua-Wei JIN ; Zhen CHEN ; Hai LAN ; Zhi-Bo XIA ; Zheng-Song HUANG
Chinese Journal of Microsurgery 2000;0(02):-
Objective To evaluate the efficacy of naloxone used in the postoperation of cerebral tumor.Methods Eighty patients were randomly assigned to receive (treated group:40 patients) or not re- ceive (control group:40 patients) naloxone.Both the two groups accepted the conventional therapy.Re- sults After operation,the content of?-EP,ET decreased continuously but the one of the treated groups was more obviously than that of the control groups (P