OBJECTIVETo optimize Supper Critical CO2 extracting technical (SFE-CO2) methods for extraction of anti-cancer active components of Fig Residues and to investigate the anti-cancer effect of the extract in vitro and in vivo.

METHODThe anti-cancer activity of extracted compound was measured on U937,95D and AGS cancer cells in vitro by MTT method. The anti-cancer effect of the extraction of Fig Residues was studied on mice transplant liver cancer in vivo.

RESULTThe SFE-CO2 condition for extraction of the anti-cancer components of Fig Residues was optimized as follows: granularity was 100, the extraction pressure was 30 MPa, the extraction temperature was 45 degrees C, the extraction time was 6 h and the CO2 flux was 12 L x h(-1); The IC50 of anti-cancer active components of Fig Residues on U937, 95D and AGS cells were 70.125 microg x mL(-1), 127.957 microg x mL(-1), 116.000 microg x mL(-1); The anti-cancer active components of Fig Residues inhibited 49.3% of the transplanted liver cancer in the mice.

CONCLUSIONThe method for extracting the anticancer active components of Fig Residues is stable and reasonable, and the extract from Fig Residues is of the anticancer effect.

Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Chromatography, Supercritical Fluid ; methods ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Ficus ; chemistry ; Fruit ; chemistry ; Humans ; Inhibitory Concentration 50 ; Liver Neoplasms ; pathology ; Male ; Mice ; Neoplasm Transplantation ; Plants, Medicinal ; chemistry ; Stomach Neoplasms ; pathology ; U937 Cells ; drug effects

Objective To examine the relationships between the level of serum visfatin and obesity,nonalcoholic fatty liver disease (NAFLD)in children and adolescents. Methods Serum visfatin levels of 106 obese children/adolescents (including 49 non-NAFLD children and adolescents and 57 NAFLD children/adolescents in which consisting of 42 mild ones,15 moderate-to-severe ones)with another 69 lean children/adolescents as their controls,were examined by indirect sandwich ELISA.Results When comparing the serum visfatin levels,there was no significant difference noticed between the obese group ( 1.71 ± 0.37) ng/ml and the lean group ( 1.75 ± 0.37) ng/ml (P=0.455).With the severity of NAFLD,the serum visfatin level showed an elevation in obese children and adolescents [ obese without NAFLD ( 1.59 ± 0.36) ng/ml,obese with mild NAFLD ( 1.74 ± 0.36) ng/ml,obese with moderate-to-severe NAFLD (1.97 ± 0.36) ng/ml,P＜0.05].In the obese group,serum visfatin levels seemed to be related to age (^β=-0.326,P=0.000),alanine aminotransferase (ALT) (^β =0.286,P=0.004) and the degree of NAFLD (^β =0.246,P＝0.014).Conclusion The level of serum visfatin was related to non-alcoholic fatty liver in obese children and adolescent.

ObjectiveTo evaluate the clinical effectiveness of posterolateral autograft bone graft fusion transpedicular screw system internal fixation and anterior debridement in the treatment of dorsal and lumber spinal tuberculosis. MethodsFrom March 1996 to July 2000,posterolateral autograft bone graft fusion transpedicular screw system internal fixation and anterior debridement procedures were used in 62 patients suffering from dorsal and lumber spinal tuberculosis in our department,48 of them were involved in a longitudinal study follow-up for a mean of 3.6(1.5-5.5)years postoperatively. ResultsAll patients showed successful posterolateral bone graft fusion. Among 38 cases of Pott's paraplegia, 30 were completely recovered,5 were partly recovered,the rate of recovery was 92.1%. The average immediate postoperative correction of kyphosis angle was 29.1°,the average loss of correction was only 3.2°at final follow-up.ConclusionsPosterolateral autograft bone graft fusion transpedicular screw system internal fixation and anterior debridement procedure were found helpful in strengthening the stability of the spine in dorsal and lumber spinal tuberculosis, providing successful interbody fusion and recovery of Pott's paraplegia, correcting the kyphosis, and preventing progression of kyphosis.

OBJECTIVETo explore the effects of iptkalim on myocardial enzymes and free radicals metabolism with hypoxic pulmonary hypertension (HPH), in order to provide evidence for the mechanism of iptkalim on clinical treat.

METHODS110 young men stayed at high altitude above 5 000 m were divided into iptkalim group (n = 74) and placebo group (n = 36), aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (gamma-GT), creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD), malonaldehyde (MDA), nitric oxide(NO) and nitric oxide synthase(NOS) were detected before and after took medicines for 6 mouths.

RESULTSAfter took medication for 6 mouths, ALT, AST, gamma-GT, CK and LDH were reduced, SOD, NO, and NOS were increased, MDA were reduced, there were very significant difference (P < 0.05).

CONCLUSIONOxygen free radicals have taken part in the process of HPH, iptkalim have the effect of anti-peroxidation of lipid and protect myocardial cells stress injured by hypoxia which related with mitochondrial membrane and cell membrane's K(ATP) channel activation.

Adolescent ; Adult ; Altitude ; Creatine Kinase ; blood ; Free Radicals ; metabolism ; Humans ; Hypertension, Pulmonary ; blood ; etiology ; metabolism ; Hypoxia ; complications ; L-Lactate Dehydrogenase ; blood ; Male ; Myocardium ; enzymology ; Potassium Channels ; agonists ; Propylamines ; pharmacology ; Young Adult

OBJECTIVETo investigate the dynamic changes of the epididymal size 1 year after vasectomy.

METHODSFifty male volunteers received vasoligation. Before and at 1, 2, 3, 6, and 12 months after operation, we measured the size and detected the internal echoes of the epididymis using color Doppler ultrasonography.

RESULTSThe bilateral epididymides were both thickened post-operatively in all the 50 cases, with statistically significant differences between the baseline and the 1st month, the 1st and the 2nd month, the 2nd and the 3rd month, or the 3rd and the 6th month after surgery (all P < 0.01), but not between the 6th and the 12th month (P > 0.05).

CONCLUSIONWithin 6 months after vasectomy, the bilateral epididymides manifested a progressive thickening, but basically restored their balance of secretion-absorption after 6 months.

Epididymis ; diagnostic imaging ; pathology ; physiology ; Humans ; Male ; Organ Size ; Postoperative Period ; Time Factors ; Ultrasonography, Doppler, Color ; Vasectomy

Objective To study the inhibitory effect of small interference RNA(siRNA) ofcyclin E gene on the growth ofK562cells. Methods siRNA targeting the 940 bp site of the cyclin E mRNA were designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA were then transfected into K562 cells via LipofectamineTM2000.The cells transfected with non-functional siRNA served as the negative control group and those only treated with serum-free RPMI1640 as the blank control group. Cell counting, reverse transcriptase (RT)-PCR and flow cytometry were employed to evaluate the effect of RNA interference. Results Compared with the negative and blank control groups, the viable cell count in the interference group was decreased by approximately 80%, the ratio of G1-phase cells increased by nearly 30%, and growth arrest was observed. Cyclin E mRNA expression in the cells of the interference group was significantly lowered by about 70%as compared with that of the negative and blank control groups, whereas the latter two groups had similar expression levels.Conclusion RNA interference induces obvious inhibition of cyclin E gene expression, which consequently affects the proliferation ofK562 cells.

Objective To study the inhibitory effect of small interference RNA(siRNA) ofcyclin E gene on the growth ofK562cells. Methods siRNA targeting the 940 bp site of the cyclin E mRNA were designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA were then transfected into K562 cells via LipofectamineTM2000.The cells transfected with non-functional siRNA served as the negative control group and those only treated with serum-free RPMI1640 as the blank control group. Cell counting, reverse transcriptase (RT)-PCR and flow cytometry were employed to evaluate the effect of RNA interference. Results Compared with the negative and blank control groups, the viable cell count in the interference group was decreased by approximately 80%, the ratio of G1-phase cells increased by nearly 30%, and growth arrest was observed. Cyclin E mRNA expression in the cells of the interference group was significantly lowered by about 70%as compared with that of the negative and blank control groups, whereas the latter two groups had similar expression levels.Conclusion RNA interference induces obvious inhibition of cyclin E gene expression, which consequently affects the proliferation ofK562 cells.

OBJECTIVETo verify the clinical efficacy of acupuncture combined with auricle cutting method for treatment of blood stasis-type psoriasis.

METHODSFifty-six cases of blood stasis-type psoriasis were randomly divided into a combined therapy group, a auricle cutting group, an acupuncture group and a control group, 14 cases in each one. Based on regular treatment of TCM decoction in four groups, the combined therapy group was treated with acupuncture and auricle cutting method, and the auricle cutting group was treated with sham-acupuncture and auricle cutting, and the acupuncture group was treated with acupuncture and sham auricle cutting, and the control group was treated with sham-acupuncture and sham auricle cutting. The acupuncture was applied at Dazhui (GV 14), Feishu (BL 13), Ganshu (BL 18) and Geshu (BL 17), etc., and manipulated with routine technique; in the sham acupuncture, the needle was inserted into dermis layer so that the needles could be swung without being dropped out. In the auricle cutting, erbeixin (P1) of unilateral auricle was selected and cut by Chan needle to perform bloodletting; in the sham auricle cutting, the neighborhood approximately 0.5 cm next to erbeixin (P) of auricle was selected as cutting area. The treatment was given once a day, seven days as a treatment session for totally two sessions. Psoriasis area and severity index (PASI) before and after treatment was observed and efficacy of each group was compared.

RESULTSThe effective rate was 57.1% (8/14) in the combined therapy group, which was superior to 14.3% (2/14) in the auricle cutting group, 7.1% (1/14) in the acupuncture group and 0.0% (0/14) in the control group (all P < 0.05). The scores of PASI were all decreased in each group after the treatment (all P < 0.05), which was the most significant in the combined therapy group (all P < 0.05). After factorial analysis, the main effect was P < 0.05 in the auricle cutting, P < 0.05 in the acupuncture and P < 0.05 in interaction effect of combined therapy.

CONCLUSIONThe scores of PASI of blood stasis-type psoriasis could be effectively reduced by acupuncture, auricle cutting method and TCM decoction, among which the interaction effect of auricle cutting and acupuncture combined with TCM decoction is the most significant.

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Bloodletting ; Combined Modality Therapy ; Ear ; blood supply ; Female ; Humans ; Male ; Middle Aged ; Psoriasis ; blood ; therapy ; Treatment Outcome ; Young Adult

OBJECTIVETo evaluate clinical outcome after anterior cruciate ligament (ACL) reconstruction with double-bundle or single-bundle by meta-analysis.

METHODSRandomized controlled trials (RCTs) on differences of clinical outcomes of ACL reconstruction were retrieved in Ovid Medline, PubMed, Embase, Cochrane Library, CBM and VIP database. Relevant journals or conference proceedings were also searched manually. Then extracted the date of KT-1000 arthrometer, pivot-shift testing, Lysholm score and IKDC final score in these researches. RevMan 5.0.23 software was used for data analyses.

RESULTSEight prospective RCTs met the inclusion criteria. The combined results of meta-analysis indicated that there was statistical difference between two operative procedures on postoperative KT-1000 arthrometer side-to-side [WMD = -0.35, 95%CI (-0.61, -0.08), P = 0.01], Lysholm score [WMD = -1.91, 95%CI (-3.45, -0.37), P = 0.01]. But the difference of KT-1000 arthrometer side-to-side is demonstrated to be clinically insignificant. Others indicated that there was no statistical differences with respect to IKDC final score [OR = 1.80, 95%CI (0.98, 3.31), P = 0.06], having a normal or nearly normal pivot-shift testing [OR = 1.64, 95%CI (0.85, 3.16), P = 0.14].

CONCLUSIONSDouble-bundle reconstruction does not result in clinically significant advantage when compared with single-bundle. The results do not support the theory that double-bundle reconstruction controls knee rotation better.

Anterior Cruciate Ligament ; surgery ; Humans ; Joint Instability ; surgery ; Reconstructive Surgical Procedures ; methods ; Treatment Outcome

OBJECTIVETo explore the association between sleep duration and the level of puberty blood pressure of students aged 9 to 17 years in China.

METHODSThe data were from physical fitness and health research of chinese school students in 2010. A total of 123 919 boys aged 11 to 17 years and girls aged 9 to 17 years were included in our study, whose record of height, weight, blood pressure, sleep duration and first spermatorrhea/menarche were performed. Students were grouped as relative enough sleep duration group and short sleep duration group by their sleep duration reported. The level of blood pressure, as well as the prevalence of high blood pressure (HBP), was compared between different sleep duration groups, and multilevel model was used and analyzed the relationship between sleep duration and blood pressure.

RESULTS123 919 students were surveyed in this study, including 52 549 boys and 71 370 girls. The mean blood pressure of boys was 109.41/67.05 mm Hg (1 mm Hg = 0.133 kPa), 30 830 boys (58.7%) experienced first spermatorrhea, and 18 692 boys (35.6%) were defined as relative enough sleep duration. The mean level blood pressure of girls surveyed was 103.65/65.12 mm Hg, 46 031 girls (64.5%) experienced menarche, and 23 143 girls (32.4%) were defined as relative enough sleep duration. Among the boys without first spermatorrhea, 7481 boys (34.4%) were defined as relative enough sleep duration, whose mean blood pressure was 105.25/65.01 mm Hg and prevalence of HBP was 4.3% (325/7481), and 14 238 boys (65.6%) were defined as short sleep duration, whose mean blood pressure was 105.9/65.27 mm Hg and prevalence of HBP was 5.6% (802/14 238), boys with relative enough sleep duration showed a significant lower blood pressure level and the prevalence of HBP compared with boys with short sleep duration (F = 15.38, P < 0.01;χ(2) = 16.55, P < 0.01). Among girls without menarche, 9311 girls (36.7%) were defined as relative enough sleep duration, whose mean blood pressure was 100.47/63.25 mm Hg and prevalence of HBP was 2.9% (271/9311), 16 028 girls (63.3%) were defined as short sleep duration, whose mean blood pressure was 101.06/63.57 mm Hg and prevalence of HBP was 3.3% (536/16 028), girls with relative enough sleep duration showed a lower level of blood pressure compared with girls with short sleep duration (F = 17.44 and 7.11, respectively, all P values < 0.01). Among girls experienced menarche, 13 832 girls (30.0%) were defined as relative enough sleep duration, whose mean blood pressure was 105.27/66.19 mm Hg and prevalence of HBP was 6.25% (864/13 832), and 32 199(70.0%) girls were defined as short sleep duration, whose mean blood pressure was 105.12/66.19 mm Hg and prevalence of HBP was 6.0% (1924/32 199), girls with relative enough sleep duration showed significant higher level of blood pressure than girls with short sleep duration (F = 5.98, P = 0.015). After adjustment of region, BMI, height, age and urban/rural area by multilevel model, the level of systolic pressure decreased 0.18 mm Hg with 1 h increase of sleep duration among boys without first spermatorrhea (Wald χ(2) = 7.27, P = 0.007), the level of diastolic pressure decreased 0.13 mm Hg with 1 h increase of sleep duration among girls without menstruation (Wald χ(2) = 5.52, P = 0.019), and the systolic pressure and diastolic pressure increased 0.19 mm Hg and 0.14 mm Hg with 1 h increase of sleep duration among girls with menstruation, respectively (Wald χ(2) = 13.47 and 9.94, respectively, all P values < 0.01).

CONCLUSIONShort sleep duration may be one of the influential factors of HBP during puberty.

Adolescent ; Adolescent Behavior ; physiology ; Blood Pressure ; Child ; China ; epidemiology ; Female ; Humans ; Hypertension ; epidemiology ; Male ; Sleep ; physiology