1.Diagnostic Value of Computed Radiography on Neonatal Respiratory Distress Syndrome
ji-cheng, DU ; hai-bin, ZHOU ; fu-chun, LI ; rui-zhen, HONG ; man-hua, BAO
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To improve the knowledge and diagnostic ability of imagiology on neonatal respiratory distress syndrome (NRDS) computed radiograph(CR).Methods The doubtful patients were done to photographs bedside using the high resolution imaging plate, 50 cases of newborn with NRDS were selected whose clinical diagnosed clearly and had been treated and had the complete CR image documents.The CR change and clinical characteristics were observed dynamically.Results Nine of 50 cases were combined with aspirated pneumonia,8 cases with infective pneumonia,3 cases with intra-alveolar hemorrage,and 2 cases with pneumothorax.Accoding to X-ray manifestations,all cases were divided into four stages:Ⅰ stage(n=5), Ⅱ stage(n=20),Ⅲ stage(n=22),Ⅳstage(n=3).Typical CR signs included:the pulmonary lucency decreasd,wide-ranging net and grain shadowes of high density, and in companing with a lot of air brunchus sing.Conclusions Computed radiography is the most important imaging method in diagnosis of NRDS bedside ,and shall be improved the ability of diagnosis and differential of NRDS combined with the clinic.
2.Study on severe acute respiratory syndrome seroepidemiology after its outbreak in a general hospital
Zhen YANG ; Hai-Ke DU ; Bin SU ; Ying JIN ; Xue-Mei DONG ; Shi-Xin WANG ;
Chinese Journal of Infectious Diseases 2000;0(02):-
Objectives To study the level and development of serum specific antibody against severe acute respiratory syndrome coronavirus(SARS-CoV)of different populations in SARS pestilence district after SARS outbreak in a general hospital.Discuss SARS sub-clinical infection and protective action of the IgG antibody.Methods Seroepidemiology method,enzyme-linked immunosorbent assay (ELISA)and indirect immunfluorescence assay(IFA)were employed to investigate the changing level of serum antibody to SARS-associated coronavirus in non-SARS population in SARS pestilence district during and after SARS outbreak.The development of IgM and IgG antibody in patients with SARS in 6 weeks after the onset of SARS was studied qualitatively.The level changing of IgG antibody in con- valescent patients with SARS in 82 weeks after the onset was observed dynamically.Results The ELISA test outcome of IgG antibody was negative in 200 non-SARS people who were random samples of normal mass in SARS pestilence district and common community.The positive rate was 0.41% in 487 SARS high risk population tested by ELISA,but showed negative when retested by IFA.The A value level of IgG antibody existed significant difference in non SARS mass during and after SARS outbreak and the later's was higher them the former's(P
3.Standardized selection of medical consumables based on PDCA cycle
Zhen-Zhen CAO ; Li YANG ; An-Hai WEI ; Zhen-Wei DU ; Hong-Dan WANG ; Qing-Hui REN
Chinese Medical Equipment Journal 2023;44(12):78-82
The problems during medical consumables selection were analyzed with a fishbone diagram.The selection process was optimized based on PDCA cycle,and a standardized selection plan for medical consumables was formulated by combining the practical experience of hospital management,and the practicability and feasibility of the selection plan was verified.References were provided for medical institutions to select cost-effective medical consumables.
4.Idiopathic pulmonary vein thrombosis extending to left atrium: a case report with a literature review .
Jun-ping WU ; Qi WU ; Yang YANG ; Zhong-zhen DU ; Hong-fen SUN
Chinese Medical Journal 2012;125(6):1197-1200
Pulmonary vein thrombosis is a rare disease and is usually represented as a complication of atrial fibrillation, pulmonary tumors, and lobectomy. Although it is a potentially life threatening condition, the venous disease is easy to misdiagnose because of the non-specific symptoms. In this article, we present a 30-year-old patient who suffered from pulmonary vein thrombosis without any causes. He was diagnosed with other pulmonary disorders till the thrombus within the pulmonary vein extended into the left atrium. Left atrium mass resection and a left lower lobectomy were undertaken with relative urgency. The postoperative course was uneventful. The patient received a long course of oral anticoagulant therapy.
Adult
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Echocardiography, Transesophageal
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Heart Atria
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pathology
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Humans
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Male
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Pulmonary Veins
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Venous Thrombosis
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pathology
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surgery
5.Differential gene expressions in the stromal cells of the human prostate peripheral zone in males of different ages.
Yong-Chuan WANG ; Jing DU ; Hai-Zhen ZUO ; Hong-Kai LU ; Hai-Tao LIU ; Bang-Min HAN ; Shu-Jie XIA
National Journal of Andrology 2012;18(12):1078-1082
OBJECTIVETo study the gene expressions in the stromal cells of the human prostate peripheral zone (PZ) in men of different ages.
METHODSWe primarily cultured stromal cells from the normal prostate PZ of men aged 23 -32 (young group) and 56 -75 years (old group), profiled the gene signature of the PZ cells by cDNA microarray, and defined the differential gene expression patterns by hierarchical cluster analysis. Among the differential genes, we selected and confirmed up-regulated genes by quantitative real time PCR (Q-PCR), and identified their protein coding by Western blotting.
RESULTSThere were significant differences in the gene expressions of the PZ cells between the old and young groups. Based on the fold change ratio of > or = 2 or < or = 0.5, 509 up-regulated and 188 down-regulated genes were selected in the PZ cells. A subset of significantly differential genes influencing the growth of adjacent epithelial cells were identified, including HGF, IGF2, IGFBP5 and MMP1 in the old males.
CONCLUSIONStromal cells in the prostate PZ were more active in older males in promoting the malignant progression of adjacent prostate epithelial cells, which might be due to the increased expression of extracellular paracrining mediators.
Adult ; Age Factors ; Aged ; Cell Proliferation ; Cells, Cultured ; Gene Expression ; Gene Expression Profiling ; Humans ; Male ; Middle Aged ; Prostate ; metabolism ; Stromal Cells ; metabolism ; Young Adult
6.Restoration of erectile function by reconstructing cavernous nerves with small intestinal submucosa grafts.
Hong-Kai LU ; Hai-Jun ZHOU ; En-Jiang GAO ; Lu-Jie SONG ; Hai-Zhen ZUO ; Bo YAN ; Zhi-Yong YU ; Jing DU ; Wen-Hua BI
National Journal of Andrology 2010;16(2):150-153
OBJECTIVETo investigate the restoration of erectile function by reconstructing cavernous nerves (CN) with small intestinal submucosa (SIS) grafts.
METHODSWe prepared SIS grafts, established rat models and divided the models into a CN ablation, a sham-operation and an SIS graft group. The CNs at both sides were severed with 1 cm ablated in the first group, and 0.5 cm removed in the third, followed by reconstruction with the SIS grafts. Three months after surgery, the apomorphine test was performed to evaluate the erectile function, and then all the rats were sacrificed to detect the expression of nNOS in the penis.
RESULTSPenile erection was observed in 72.73% (8/11) of the rats for (1.07 +/- 0.89) times within 30 min in the SIS graft group, as compared with 0% (0/11) of the rats for (0.00 +/- 0.00) times in the CN ablation group (P < 0.01), and 90.91% (10/11) of the rats for (2.19 +/- 1.17) times in the sham-operation group (P < 0.01). The number of nNOS nerve fibers was significantly larger in the SIS graft than in the CN ablation group (70.36 +/- 10.09 versus 22.09 +/- 4.76, P < 0.01), but both were significantly smaller than that of the sham-operation group (90.81 +/- 5.69, P < 0.01).
CONCLUSIONThe SIS grafting technique contributes to the recanalization of the severed CN and restoration of erectile function in rats after surgical injury.
Animals ; Erectile Dysfunction ; surgery ; Intestinal Mucosa ; transplantation ; Intestine, Small ; Male ; Nerve Regeneration ; Nerve Tissue ; injuries ; surgery ; Penile Erection ; Penis ; innervation ; Rats ; Rats, Sprague-Dawley
7.Tunicamycin enhances TRAIL-induced apoptosis by inhibition of cyclin D1 and the subsequent downregulation of survivin.
Hai Yan ZHANG ; Zhen Xian DU ; Bao Qin LIU ; Yan Yan GAO ; Xin MENG ; Yifu GUAN ; Wei Wei DENG ; Hua Qin WANG
Experimental & Molecular Medicine 2009;41(5):362-369
TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.
Anti-Bacterial Agents/*pharmacology
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*Apoptosis
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Cell Line, Tumor
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Cyclin D1/*antagonists & inhibitors/metabolism
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*Down-Regulation
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Humans
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Microtubule-Associated Proteins/*genetics/metabolism
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TNF-Related Apoptosis-Inducing Ligand/*metabolism
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Tunicamycin/*pharmacology
8.Tunicamycin enhances TRAIL-induced apoptosis by inhibition of cyclin D1 and the subsequent downregulation of survivin.
Hai Yan ZHANG ; Zhen Xian DU ; Bao Qin LIU ; Yan Yan GAO ; Xin MENG ; Yifu GUAN ; Wei Wei DENG ; Hua Qin WANG
Experimental & Molecular Medicine 2009;41(5):362-369
TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.
Anti-Bacterial Agents/*pharmacology
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*Apoptosis
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Cell Line, Tumor
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Cyclin D1/*antagonists & inhibitors/metabolism
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*Down-Regulation
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Humans
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Microtubule-Associated Proteins/*genetics/metabolism
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TNF-Related Apoptosis-Inducing Ligand/*metabolism
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Tunicamycin/*pharmacology
9.Antisurvivin oligonucleotides inhibit growth and induce apoptosis in human medullary thyroid carcinoma cells.
Zhen Xian DU ; Hai Yan ZHANG ; Da Xin GAO ; Hua Qin WANG ; Yong Jun LI ; Guo Liang LIU
Experimental & Molecular Medicine 2006;38(3):230-240
Suvivin is a novel member of the inhibitor of apoptosis protein (IAP) family, which is known to be over-expressed in various carcinomas and associated with their biologically aggressive characteristics. The aim of this study was to investigate survivin expression in human medullary thyroid carcinoma (MTC) and a MTC cell line TT, correlate suvivin expression with clinicopathologic features of MTC, and test effects of antisurvivin oligonucleotides (ASODNs) on growth and apoptosis of TT cells. Survivin expression was immunohistochemically determined in formalin-fixed and paraffin-embedded specimens obtained from 10 cases of normal thyroid (NT) and 10 cases of MTC, and in TT cells. In TT cells, we confirmed survivin expression and its down-regulation by ASODNs using RT-PCR and Western blot analyses, and investigated effects of ASODNs on viability and growth by MTT assay and apoptosis by apoptotic analyses including DNA laddering assay, acridine orange/ethidium bromide staining and flow cytometric cell cycle analysis. Immunohistochemical analysis showed high survivin expression in MTC and TT cells, whereas no immunoreactivity was detectable in NT. Statistical analyses revealed no significant correlation of survivin expression with the clinicopathologic features of MTC. In TT cells, survivin expression at both mRNA and protein levels was confirmed and could be down-regulated by ASODNs concomitant with decrease in viability and growth, and increase in apoptosis. Our results suggest that survivin plays an important role in MTC independent of the conventional clinicopathologic factors, and ASODNs is a promising survivin-targeted gene therapy for MTC.
Time Factors
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Thyroid Neoplasms/*metabolism/pathology
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Reverse Transcriptase Polymerase Chain Reaction
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Oligonucleotides, Antisense/genetics/*pharmacology
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Neoplasm Proteins/genetics/*metabolism
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Microtubule-Associated Proteins/genetics/*metabolism
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Male
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Humans
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Gene Expression Regulation, Neoplastic/drug effects
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Female
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Down-Regulation/drug effects/genetics
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Dose-Response Relationship, Drug
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Cell Survival/drug effects
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Cell Proliferation/*drug effects
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Cell Line, Tumor
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Carcinoma, Medullary/*metabolism/pathology
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Apoptosis/*drug effects
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Adult
10.A research of endothelial cell-targeted therapy for cure of hypertrophic scar.
Rui SHEN ; Tian-zeng LI ; Shao-hai QI ; Hui-zhen LIANG ; Ying-bin XU ; Ju-lin XIE ; Yi-fang WU ; Xiang-sheng FENG ; Yong-jun DU
Chinese Journal of Plastic Surgery 2003;19(4):254-257
OBJECTIVETo investigate the feasibility of endothelial cell-targeted therapy to cure post-burn hypertrophic scar.
METHODSA hypertrophic scar animal model was made. Intralesional injecting of VEGF monoclonal antibody was performed for three weeks. The changes of scar in volume and morphology were observed.
RESULTS1. The volume of scar decreased. 2. The number of the capillary, the amount of collagen I and collagen III decreased. 3. Transmission electron microscope examinations demonstrated many dead or apoptotic fibroblasts and endothelial cells. Fibrocytes were seen relatively common.
CONCLUSIONVEGF induces the growth and development of hypertrophic scar in that it induces excessive and uncontrollable angiogenesis, which favors excessive collagen synthesis. Endothelial cell-targeted therapy may be a promising method to cure post-burn hypertrophic scar.
Animals ; Apoptosis ; Burns ; complications ; Cicatrix, Hypertrophic ; chemically induced ; therapy ; Collagen Type I ; Collagen Type III ; Disease Models, Animal ; Endothelial Cells ; Feasibility Studies ; Neovascularization, Pathologic ; etiology ; Vascular Endothelial Growth Factor A