Acute Disease ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; chemically induced ; Trimethyltin Compounds ; poisoning

Objective To study the feasibility of improving pre-operative bowel preparation in colorectal cancer patients. Methods Sixty cases were randomly divided into two groups with thirty cases in each group. The experimental group was performed oral magnesium sulfate combination with glucose normal saline the day before surgery while the control group was conducted the ordinary pre-operative bowel preparation.All of the cases were tested for peripheral blood hemoglobin concentration, hematocrit and part of the serum ion levels before and after bowel preparation. Weight was measured and intestinal flora was analyzed before and after surgery preparation. The number of diarrhea was counted in the process of bowel preparation and intestinal cleanliness was observed during operation. Patients' general condition and complications were observed before and after surgery. Results Compared with the control group, the experimental group outperformed in terms of bowel preparation tolerance and the number of preoperative diarrhea(P＜0.05). Blood tests indicated that there was no significant difference in intestinal cleanliness between the two groups (P＞0.05). The incidence of postoperative complications in the control group was higher than that in the experimental group, which, however,was not significant (P＞0.05). In the experimental group, no significant change of intestinal flora was observed, whereas intestinal flora changed significantly in the control group. Conclusions The revised bowel preparation for colorectal cancer patients is simple. It can shorten the process of bowel preparation, increase patients' tolerance, and prevent postoperative flora disorder.

OBJECTIVETo compare the advantages and disadvantages of TFC/BAK, Prospace and Brantigan I/F carbon cages and summarize their applications.

METHODSOne hundred and twelve cases of Brantigan I/F, 123 cases of TFC/BAK and 45 cases of Prospaces were used in the treatment of degenerated lumbar disorders. The clinical results and complications were observed and analyzed.

RESULTSAll devices restored the height of intervertebral space and achieved immediate stability after insertion. One hundred and twelve cases of Brantigan I/F cages were solid fused, the clinical results were satisfactory. Three cases of TFC/BAK cages subsided into the vertebra and 2 cages retropulsed slightly during follow-up. There was no serious complications of nervous system, but the operation time and amount of bleeding in Brantigan I/F group increased.

CONCLUSIONSCarbon fiber intervertebral cages are an effective method in treating degenerated lumbar disorders. To avoid complications, the indications and techniques must be selected carefully.

Adult ; Aged ; Female ; Humans ; Lumbar Vertebrae ; surgery ; Male ; Middle Aged ; Orthopedic Fixation Devices ; Retrospective Studies ; Spinal Diseases ; surgery ; Spinal Fusion ; instrumentation ; methods ; Treatment Outcome

OBJECTIVESTo evaluate the effect of intervertebral carbon fiber cages in the treatment of degenerated lumbar disorders.

METHODSOne hundred and twelve patients of degenerated lumbar disease were analyzed retrospectively with regard to the effect of posterior lumbar interbody fusion (PLIF) using intervertebral carbon fiber cage.

RESULTSIn the 112 patients, disc height was 7 mm (3 to 11 mm) on average before operation and 11.2 mm (8 - 14 mm) after operation. Cobb angle was 32 degrees (21 degrees to 42 degrees ) on average before operation and 18.9 degrees (13 degrees to 21 degrees ) after operation. After follow-up for 7.8 months (4 to 13 months) on average, all operative disc spaces showed solid arthrodesis through X-ray images. Disc height was 10.9 mm (8 - 14 mm) on average, and Cobb angle was 18.5 degrees (13 degrees to 21 degrees ) on average. During the follow-up, endplate absorbing, pseudo-articulation, cage displacement, subsidence, and breaking were not found.

CONCLUSIONImplantation of intervertebral carbon fiber cages is an effective method for the treatment of lumbar degenerative disease. Accurate indications and skilled techniques are required to avoid complication.

Aged ; Carbon ; Female ; Follow-Up Studies ; Humans ; Internal Fixators ; Lumbar Vertebrae ; Male ; Middle Aged ; Retrospective Studies ; Spinal Diseases ; surgery ; Spinal Fusion ; instrumentation ; methods ; Treatment Outcome

OBJECTIVETo investigate the effect of edaravone on renal ischemia-reperfusion injury in rats.

METHODSFifty rats were randomly divided into five groups: sham operation group (Group A), renal ischemia-reperfusion group (Group B) and edaravone treated groups (Group C1, Group C2 and Group C3 with different drug dosages). Serum maleic dialdehyde (MDA) and superoxide dismutase (SOD), renal MDA and SOD, serum creatinine (Cr), blood urea nitrogen (BUN) were measured after the rat kidney was ischemia-reperfused for 24 hours. Renal ultrastructure was observed.

RESULTCompared with Group A, serum and renal MDA, serum Cr, BUN of Group B were significant increased (P <0.01), serum and renal SOD of Group B were significant decreased (P <0.01). After edaravone treatment, serum MDA, Cr and renal MDA of Group C were lower than those in Group B (P<0.01); Serum and renal SOD of group C were higher than those in Group B (P <0.01); Compared with Group B, BUN level of Group C had no significant change (P >0.05). The renal ultrastructure was greatly injured in Group B, meanwhile it was obviously ameliorated in Group C.

CONCLUSIONEdaravone has protective effects on renal ischemia-reperfusion injury in rats.

Animals ; Antipyrine ; analogs & derivatives ; therapeutic use ; Kidney ; blood supply ; Male ; Malondialdehyde ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; metabolism ; prevention & control ; Superoxide Dismutase ; metabolism

OBJECTIVETo investigate the therapeutic effects of double filtration plasmapheresis(DFPP) in treatment of hyperlipidemic acute pancreatitis.

METHODSNine patients with acute hyperlipidemic pancreatitis were treated with DFPP in addition to the conventional therapeutic measures. The clinical symptoms,serum levels of triglyceride (TG) and APACHE II scores were observed before and after DFPP.

RESULTAfter DFPP the clinical symptoms of patients were improved greatly; serum levels of TG decreased from (83.48 +/-2.54)mmol/L to (4.09 +/-0.65)mmol/L(P<0.01) and APACHE II scores decreased from 12.2 +/- 2.3 to 6.2 +/- 1.3(P <0.05). There were no significant side effects during and after DFPP.

CONCLUSIONDFPP can be effectively and safely applied in patients with acute hyperlipidemic pancreatitis.

Acute Disease ; Adult ; Female ; Filtration ; methods ; Hemofiltration ; methods ; Humans ; Hyperlipidemias ; etiology ; therapy ; Male ; Middle Aged ; Pancreatitis ; complications ; therapy ; Pancreatitis, Acute Necrotizing ; therapy ; Plasmapheresis ; instrumentation ; Young Adult

Mitochondrion is an organelle containing its own genome in eukaryotic cells , which encodes 37 genes involved in mitochondrial functions .Mitoepigenetic regulation is a major form of mitochondrial genome-encoded genes that regulates the expression levels without altering the sequences of the genes .The mitoepigenetic regulation is involved in the occurrence and development of various diseases .This paper reviews the progress of mitoepigenetic regulation and Alzhe-imer disease.

Adult ; Hematoma, Epidural, Spinal ; etiology ; Humans ; Male ; Spinal Fractures ; complications ; surgery ; Thoracic Vertebrae ; injuries ; surgery

BackgroundAn ideal animal model is very important for the investigation of the immune mechanism of high-risk rejection corneal transplantation.ObjectiveThis study was to compare three methods of creating a high-risk corneal transplantation model in rabbits to study high-risk rejection corneal transplantation.MethodsForty-five New Zealand white rabbits were utilized and assigned randomly to three groups of different modeling methods,with 15 rabbits for each group.The high-risk corneal transplantation models were created by suturing with 5-0 silk thread in 4 quadrants,inducing alkali burn with 1 mol/L NaOH or corneal xenotransplantation.In the suturing group and alkali burning group,the rabbits received a unilateral 7.25 mm diameter corneal allograft after corneal neovascularization was induced,and in the xenotransplantation group,corneas from cats were used as donors.Rabbits were followed-up for 4 weeks in all groups.Corneal neovascular area was calculated and compared among the three groups.The amount of rejection,inflammatory index ( IF),neovascularization and histology of grafts were clinically scored to calculate the reject index (RI).ResultsThere were 14,15 and 15 rabbits that survived the high-risk penetrating corneal transplantation,respectively,in the suturing group,alkali burning group and xenotransplantation group.Two weeks after operation,the IF scores were 0.543 ± 0.103,0.811 ± 0.054 and 0.191 ±0.087,and the RI were 2.111±0.928,7.0±0.816 and 3.182±0.751 in the suturing group,alkali burning group and xenotransplantationgroup,respectively,showingstatisticallysignificantdifferencesamongthethreegroups (x2 =25.736,22.432,P =0.000).The IF value was lower in the xenotransplantation group compared with the suturing group and alkali burning group (Z =3.841,3.993,P =0.000),and that of the suturing group was lower than the alkali burning group (Z =3.568,P =0.000).The RI value of the xenotransplantation group was significantly raised in comparison with the suturing group and declined in comparison with the alkali burning group (Z =2.373,P =0.018;Z =3.936,P =0.000),and that of the suturing group was lower than the alkali burning group (Z =3.729,P =0.000 ).The survival times of the grafts were ( 17.9±2.0 ) days,( 13.4 ±2.4) days and ( 15.5 ±2.0 ) days in these three groups with a significant difference among them ( F =9.474,P =0.001 ).The neovascularization area in the xenotransplantation group was smaller than the suturing group and alkali burning group (P＜ 0.05 ).Histological examination revealed a large number of inflammatory cells infiltration in the grafts 2 and 4 weeks after transplantation in the suturing group and alkali burning group,but less inflammatory cells were seen in the xenotransplantaion group.Immunofluorescence staining showed abundant CD4+ T positive cells in the grafts in the three groups.Conclusions The cat-rabbit corneal xenotransplantation can induce stable and moderate immune rejection.This animal model has milder inflammatory response and less corneal neovascularization than the suture and alkali burn models.This method therefore is an ideal model for high-risk corneal transplantation.

OBJECTIVESTo investigate the techniques of posterior lumbar interbody fusion and pedicle screws in the treatment of degenerative scoliosis and evaluate their clinical significance.

METHODSTwenty five patients with degenerative scoliosis were treated operatively during 1997 - 2002. Among the 25 patients, 13 patients were treated with Brantigan I/F carbon cages in 28 intervertebral spaces, 7 patients were treated with Prospace cages in 15 spaces, 1 patient was treated with CONTACT cages in 2 spaces. The correcting effects, complications and follow-up results were analyzed retrospectively.

RESULTSThe average correction rate of scoliosis was 59% and the rate of pain relief was 88%. There was a case of pseudoarthritis and a case of embolism. During follow-up, correction rate and height of disc spaces were not lost nor shift of interbody cages.

CONCLUSIONPosterior pedicle screws combined with interbody fusion is a safe and effective treatment for degenerative scoliosis.

Adult ; Aged ; Bone Screws ; Female ; Humans ; Internal Fixators ; Male ; Middle Aged ; Retrospective Studies ; Scoliosis ; surgery ; Spinal Fusion