1.Study on Function of Osteoclast in Infants and Toddlers
hai-he, YANG ; xia, LIU ; hui-feng, ZHANG ; yuan, CHEN
Journal of Applied Clinical Pediatrics 2006;0(13):-
Objective To investigate the osteoclast′s function levels in infants and toddlers and the relationship between the osteoclast function and sex,age,body length,body weight and body mass index(BMI).Methods Sixty-eight children(37 boys and 31 girls,aged from 1 to 36 months) were studied.All of the children were in good health.These children were divided as infants group and toddlers group according to their age.Just before the samples were collected,the children′s body weight,body length were measured and the BMI were calculated.Two biochemical markers,such as serum tartrate-resistant acid phosphatase 5b(TRAP5b) and urine deoxypyridinoline(DPD) were measured.Results The difference of serum TRAP5b concentration between infants and toddlers was significant at the level of P
2.Analysis of biofilm formation ability and related genes of Staphylococcus aureus isolated from urinary tract infections
Fengjun SUN ; Hai HONG ; Wei FENG ; Yixuan SUN ; Peiyuan XIA
Chongqing Medicine 2016;45(19):2617-2619
Objective To study the biofilm formation ability and related gene distribution of Staphylococcus (S .) aureus iso‐lated from urinary tract infections to provide the theoretical basis for the prevention and treatment of clinical infection .Methods The minimal inhibitory concentration was detected using the agar double dilution method .The bacterial adhesion ability was deter‐mined by flat colony counting method .The biofilm formation ability was analyzed by the 96‐well crystal violet staining method .The biofilm‐associated genes were detected by PCR amplification .Results Eleven clinical strains of S .aureus were high resistant to pen‐icillin and erythromycin ,whereas were all sensitive to vancomycin and nitrofurantoin .All the isolates had a strong ability of adhe‐sion ,but the biofilm formation ability was weak .Among them ,the icaAD and icaBC genes were amplified in 10 S .aureus isolates . Conclusion The adhesion ability and biofilm formation ability of S .aureus isolated from urinary tract infections have the strain differences ,and ica is an important gene of S .aureus biofilm formation .
3.Advance in the Post-stroke Depression(review)
Hai-xia Feng ; Xiu-e SHI ; Xing-wu WANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(11):931-933
Post-stroke depression(PSD) is one of the most common complications after stroke,and seriously influenced the quality of life of hemiplegic patients.The article would review the associated factors and mechanism of PSD.
5.Gastric adenocarcinoma associated with myelolipoma of parietal pleura: a case report.
Dong-sheng ZHANG ; Dong-hai LI ; Hai-xia BI ; Xiao-feng WU
Chinese Journal of Pathology 2006;35(7):437-437
Adenocarcinoma
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pathology
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surgery
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Biopsy
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Diagnosis, Differential
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Gastric Fundus
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pathology
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surgery
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Humans
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Male
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Middle Aged
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Myelolipoma
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pathology
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surgery
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Neoplasms, Multiple Primary
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pathology
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surgery
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Pleural Neoplasms
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pathology
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surgery
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Stomach Neoplasms
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pathology
;
surgery
6.Application of electromyographic biofeedback training in the treatment of urinary incontinence
Hai-Xia FENG ; Xue-Song LU ; Hong-Yun QIN ; Bei-Bei WANG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(05):-
Objective To study effect of eleetromyograghie(EMG) biofeedback training on three kinds of u- rinary ineontifienee.Methods Nineteen patients with urinary incontinence were treated by means of EMG biofeed- back training twice a day for six weeks.The treatment was performed with a device,which can detect the EMG ampli- tude of the pelvic muscle and deliver electric stimulation accordingly.Results After 6 weeks of treatment,the inci- dence of uretbrorrhea was reduced by 41%,and the frequency of micturition was decreased by 38% ,while the fre- quency of urination in one day reduced to 9 to 13.The general subjectively rated improvement rate of patients was 53% ,while the general objectively one was 58%.Conclusion Biofeedback training has significant therapeutic: eftects on patients with urinary incontinence.
7.EFFECTS OF SUBSTRATE DISPERSION AND DISSOLUTION ON MICROBIAL ENZYMATIC CONVERSION OF STEROID
Kui YANG ; Xiao-Jing LI ; Xia FENG ; Qiang YIN ; Hai-Yan ZHAO ;
Microbiology 1992;0(06):-
The dissolution of substrate is the key step in steroid microbial conversion.The formation of surfactant micelle with a hydrophobic center directly results in increased pseudo-water-solubilities of hydrophobic compounds such as steroids.Results showed increased water solubility of steroid with the increased surfactant concentrations.Compared to mono-surfactant PSE,less quantity of multiple surfactants PSE-MGE was required to achieve the enhancement of steroid solubility at the same level,thus the negative effects of surfactants on mycelial growth were reduced and steroid conversion was therefore improved more.It was also shown that the surfactants and ultrasonic irradiation used jointly could result in better substrate dispersion and dissolution than surfactants used alone,and the best result was obtained when the comparatively high intensity (12.4 W/cm 2) and short time (5min) of ultrasonic irradiation were performed.
8.Isolation and Identification of Thermoacidiphilic Bacteria from Apple Juice Concentrate
Feng WANG ; Jian-Ke LI ; Yu-Rong GUO ; Hai-Xia LIU ;
Microbiology 2008;0(11):-
The thermotolerant bacteria which was isolated from the Apple Juice Concentrate(AJC) was investigated by comparison with the standard strain of Aliyclobacillus acidoterrestris DSM3922 using Kirin-medium acidified with malic acid.The results shows the two polluted bacteria isolated from AJC can grow under the temperature of 21?C~55?C and pH of 2.4~6.2,which was corresponded with the characteristics of the thermo-acidiphilic Alicyclobacillus spp..Further more,the cell morphology,colony morphology,cultural characteristics and physiological characteristics tests indicated the two isolated strains of thermotolerant bacteria have obviously similar characteristics with the standard strain of Aliyclobacillus acidoterrestris DSM3922.
9.Identification of the Polyreactivity of Monoclonal Natural Anti-ker atin Autoantibody 3B4 and Analysis of Its Molecular Mechanism
Hai-Hong GUAN ; Meng FU ; Ru-Shan XIA ; Gang WANG ; Yu-Feng LIU ;
Chinese Journal of Dermatology 1994;0(06):-
Objective To identify the polyreactivity of a monoclonal natural anti-keratin autoantibody 3B4 and to analyze its possible molecular me chanism.Methods enzyme-linked immunosorbent assay (ELISA)and immunohistoche mistry were applied to test the binding reactivity of 3B4 against different anti gens and tissues.The variable region genes and their amino acid composition wer e sequenced.Results 3B4 could reacted with a range of antigens and tissues,i n addition to keratin and skin.The variable region genes of its light chain and heavy chain showed high homology with germline genes VK1 am4 and VH1 J558.42.H CDR3 region,which mainly composed of short side chain amino acids(from 294 to 324 nucleotides around the heavy chain),was the only motif that differs from ot her highly homologous immunoglobulin genes.Conclusions The monoclonal natural anti-keratin autoantibody 3B4,with its variable region genes highly homologo us to germline genes,is highly polyreactive.The flexibility of HCDR3 may contr ibute to the polyreactivity.
10.Cases analysis and clinical classification of oligodontia.
Chinese Journal of Stomatology 2003;38(4):266-268
OBJECTIVETo classify the clinical symptoms of oligodontia and summarize the characteristics of the diseases.
METHODS33 patients with congenitally permanent teeth missing (6 or more than 6) were taken clinical examination, diagnosis, prosthetic treatment and made records of the status of tooth agenesis, systemic symptoms, and family history.
RESULTSThe symptoms of oligodontia were clinically classified into two groups: oligodontia with or without systemic syndromes. And the former has two typical kinds of diseases: anhidrotic/hypohidrotic ectodermal dysplasia (EDA) and Rieger syndrome.
CONCLUSIONSCorrect diagnosis and early treatment for these patients are of very importance.
Adolescent ; Adult ; Anodontia ; diagnosis ; therapy ; Child ; Child, Preschool ; Ectodermal Dysplasia ; diagnosis ; Eye Abnormalities ; diagnosis ; Female ; Humans ; Male ; Middle Aged ; Syndrome