1.Effects of 3 carbohydrates on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene
Zhiyu ZHAO ; Hai WEN ; Sunxiao CHEN
Academic Journal of Second Military Medical University 1982;0(02):-
Objective:To study the effects of glucose,mannose and galactose on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene.Methods: Yeast cells were transfected with plasmid containing a 951 bp length of 5′ upstream flanking sequence of CAP10 coding region and a reporter gene,chloramphenicol acetyl transferase(CAT);the transfectants were treated with different concentrations(10,20,40,60,80 mg/ml) of glucose,mannose and galactose.CAT activity was assessed by ELISA method and CAT activities of different groups were compared.Results: Different concentrations of glucose and mannose had no obvious influence on CAT activity;different concentrations of galactose had obvious influence on CAT activity and the influence was positively dependent with its dose within the experimental concentration range.Conclusion: Glucose and mannose have no obvious effect on the activity of CAP10 promoter;galactose has obvious inductive effect on activity of CAP10 promoter,suggesting that CAP10 gene might be related with galactose metabolism.
2.The promotion effect of lymphocyte in patients with psoriasis on proliferation of keratinocytes
Sunxiao CHEN ; Hai WEN ; Anmei DENG
Chinese Journal of Immunology 1985;0(03):-
Objective:To analyze the effect of lymphocyte in patients with psoriasis on proliferation of keratinocytes.Methods:Lymphocytes in lesion and peripheral blood were isolated and multiplied,then cultured together with normal keratinocytes. By MTT method,the living cells were counted in the mixed culture.Results:Compared with normal controls, lymphocytes in lesion and peripheral blood of psoriasis both promote the proliferation of keratinocyte(P
6.A preliminary study of the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin
Hong SANG ; Wanqing LIAO ; Jianghan CHEN ; Hai WEN
Journal of Medical Postgraduates 2001;14(2):103-105
Objectives:To study the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin.Methods:By comparing wild types(Mel+) and mutants(Mel-),we detected C.neoformans mutant laccase (CNLAC1) gene by PCR.We also cloned gene of the CNLAC1 containing about 450bp in length.E.coli DH5α was the host strain for the plasmid containing the DNA clone.Results:No deletion was found in wild type.However,CNLAC1 deletion (about 450 bp) was present (between 1 715 bp and 2 617 bp).CNLAC1 containing about 450 bp in length was cloned successfully.Conclusions:The present study was helpful in the biochemical and molecular study of CNLAC1 mutation.It might also provide us a target gene for the prognosis and gene therapy of Cryptocosis.
7.Clinical application of RapIDYeast Plus system in identifying 150 clinically common yeasts
Hong XU ; Hai WEN ; Jianghan CHEN ; Xin HUANG
Academic Journal of Second Military Medical University 1982;0(02):-
Objective:To study the role of RapIDYeast Plus (RYP) system in identifying common clinical yeasts.Methods: The target strains were cultured and passaged twice on Sabouraud dextrose agar,and were fed to RYP system after 24-48 h incubation at 30℃.Results: One hundred and thirty-nine of the 150 target strains were identified to the level of species correctly,and 8 undetermined strains were confirmed by additional tests.It was found that 3 strains had been incorrectly identified by RYP system.The accuracy of RYP system was calculated as 92% without additional tests and 98% with additional tests.Conclusion: RYP system is suitable for routine tests in clinical microbiological laboratory;it can accurately identify more than 40 kinds of yeasts and yeast-like bacteria in clinical practice.
8.Susceptibility testing of yeasts to combination of amphotericin B and 5-flucytosine in vitro according to M27-A method
Hong CHEN ; Hai WEN ; Hong XU ; Wanqing LIAO
Academic Journal of Second Military Medical University 1981;0(04):-
This article is to study the interaction between amphotericin B and 5-flucytosine to yeasts in vitro. The checkerboard microdilution method was applied to study in vitro interaction according to M27-A method of NCCLS. Twenty-eight strains of yeasts were used for combination study. Compared with the single agents,there were significant reductions in the geometric mean of the minimal inhibitory concentration of the combination(P
9.Susceptibility Testing of 28 Strains of Yeasts to the Combination of Antifungal Drugs in vitro
Hong CHEN ; Hai WEN ; Hong XU ; Wanqing LIAO
Chinese Journal of Dermatology 1995;0(03):-
Objective To study the interaction between antifungal drugs to yeasts in vitro.Methods The in vitro interaction of antifungal drugs was detected with checkerboard microdilution method based on M27-A of NCCLS for28strains of yeasts.Results There was a significant reduction in the geometric means of MICs by the combinations of antifungal drugs to the yeasts compared with the individual agents(P
10.Correlation between immune response to hepatitis B vaccine and HLA DRB1*07 DRB1*04 DRB1*1001.DQB1*0401 genes in Han population in Ningbo
Jin-Feng ZHAO ; Hai-Tang CHEN ; Guang-Wen CAO ;
Academic Journal of Second Military Medical University 2000;0(08):-
Objective:To study the correlation between responses to Hepatitis B and HLA DRB1*07 DRB1*04 DRB1*1001. DQB1*0401 genes in Han population in Ningbo.Methods:A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine.The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to tile results:negative responses group(n=120.anti-HBs D