1.Effect of diameter-controlled Ti-TiO2 nanotubes on the adhesion of osteoblast and fibroblast.
Hong-cai LI ; Yu-mei ZHANG ; Hai-ping SUN
Chinese Journal of Stomatology 2012;47(2):122-126
OBJECTIVETo study the effect of Ti-TiO2 nanotubes with different diameters on the adhesion of fibroblast and osteoblast, and to find which diameter was more favorable for cells' respective adhesion.
METHODSPure titanium sheets were polished and then anodized at different potentials for 1 h with Ti as anode and Pt as cathode. TiO2 nanotubes formed at 1, 5, 10 and 20 V potentials served as experimental groups and polished pure titanium served as control group. Field emission scanning electron microscopy (Fe-SEM) was used to analyze the surface topography. Stained nucleus with Hoechst33342 were used to measure the cell adhesion. The cell shape on the sample surface were analyzed with Fe-SEM.
RESULTSTiO2 nanotube array of different inner diameters from 15 nm to 100 nm were grown on titanium sheets by anodization at potentials from 1 to 20 V. At 30, 60 and 120 min, fibroblast adhesion at nanotubes anodized at 5 V was (141 ± 9), (388 ± 14) and (489 ± 15) respectively, significantly less than any other nanotube surface at the same time (P < 0.01). Nanotubes anodized at 20 V had the least inhibitory effect for fibroblast adhesion with a number of (579 ± 14) at 120 min, and the cell shape was also inhibited. At 30, 60 and 120 min, osteoblast had a significant better adhesion on nanotubes formed at 5 V than it did on any other surface at the same time (P < 0.01), except the control group at 30 min, with the adhesion number of (198 ± 10), (431 ± 10) and (501 ± 10) respectively, and osteoblast had a abundant spread on nanotubes formed at 5 V; while osteoblast adhesion on nanotubes anodized at 20 V was (152 ± 11), (403 ± 9) and (465 ± 12) respectively, less than on any other nanotube surface within the same time (P < 0.05), and the cell shape on the surface changed to be more elongate.
CONCLUSIONSFibroblast adhesion is inhabited more or less on Ti-TiO2 nanotubes of different diameters. Nanotubes formed at 5 V have the most osteoblast adhesion, and inhibit fibroblast adhesion.
Animals ; Cell Adhesion ; Fibroblasts ; cytology ; ultrastructure ; Mice ; Microscopy, Electron, Scanning ; Nanotubes ; chemistry ; Osteoblasts ; cytology ; ultrastructure ; Surface Properties ; Titanium ; chemistry
2.Sinomenine in combination with CsA prolonged survival of rat cardiac allograft
Hai-Dong WANG ; Hong-Sheng ZHANG ; Ping ZHANG ;
Chinese Journal of Organ Transplantation 2005;0(08):-
0.05).The combined use of sinomenine and CsA could prolong graft survival significantly(groups 4 vs groups 1,2,3,5)(P
3.Changes of tumor necrosis factor-? levels in serum and cerebrospinal fluid of childhood acute leukemia before and after treatment
ya-ping, YU ; ji-hong, YANG ; yuan-feng, FU ; ping, SHI ; hai-ning, LIU ; yong-ping, ZHAI ; guo-hong, WANG
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To explore the changes of tumor necrosis factor-?(TNF-?) in serum and cerebrospinal fluid(CSF) of children with acute lymphoblastic leukemia (ALL) and acute myelogenous leukemia(AML) and its clinical significance.Methods TNF-? in serum and CSF were measured by radioimmunoassay and CSF samples were obtained from 31 cases of childhood acute leukemia before treatment, on complete remission(CR), and continuous CR.Results Serum TNF-? was in ALL and AML before treatment [(24.35?4.84) pmol/L and(28.65?5.12) pmol/ L],which were significantly higher than those of healthy controls[(11.2 8? 1.69) pmol/L, P
4.Effect of Fuzheng Huayu recipe on CYP450 isozymes in normal and liver fibrosis rats.
Tian-hui ZHENG ; Wei LIU ; Shu-ping LI ; Tao YANG ; Chang-hong WANG ; Cheng-hai LIU
China Journal of Chinese Materia Medica 2015;40(6):1166-1172
To study the effect of Fuzheng Huayu recipe (FZHY) on five types of isozymes of cytochrome P450 (CYP450) of normal and liver fibrosis rats by using the cocktail probe method. Dimethylnitrosamine ( DMN) was injected to induce the liver fibrosis model. After the tail vein injection with Cocktail probe solutions prepared with five CYP450s probe substrates (phenacetin-CYP1A2, omeprazole-CYP2C9, tolbutamide-CYP2C19, dextromethorphan-CYP2D6, midazolam-CYP3A4), the plasma concentrations of the five probe substrates were determined by LC-MS/MS, and the pharmacokinetic parameters were calculated by PK solutions 2. After the oral administration with FZHY, normal rats given phenacetin, omeprazole, tolbutamide and dextromethorphan showed increase in AUC(0-t) and decrease in CL to varying degrees, indicating that FZHY obviously inhibited the activities of CYP1A2, CYP2C9, CYP2C19 and CYP2D6 in normal rats, but with no obvious effect on the activity of CYP3A4. After the oral administration with FZHY, liver fibrosis rats treated with CYP2C9 showed the significant increase in AUC(0-t) and significant decrease in Vd, hut with no obvious changes in the pharmacokinetic parameters of other four types of prove substances, suggesting that FZHY could significantly inhibit the activity of CYP2C9 in rats but had no effect on the activities of CYP1A2, CYP2C19, CYP2D6 and CYP3A4. The changes in the activity of CYP450 isozymes in liver fibrosis rats may be the reason for FZHY's different effects on CYP450 isozymes in normal and liver fibrosis rats.
Animals
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Cytochrome P-450 Enzyme System
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genetics
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metabolism
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Disease Models, Animal
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
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pharmacokinetics
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Humans
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Isoenzymes
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genetics
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metabolism
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Liver Cirrhosis
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drug therapy
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enzymology
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genetics
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Male
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Mass Spectrometry
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Rats
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Rats, Wistar
5.Expression and significance of erythropoietin in human gastric carcinoma on tissue microarry.
Chen JIANG ; Jian-xian YU ; Hua CHEN ; Hong-jun WEI ; Hai-yan MA ; Ping JI
Chinese Journal of Pathology 2006;35(9):559-560
Adenocarcinoma
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metabolism
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pathology
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Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Aged
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Aged, 80 and over
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Erythropoietin
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metabolism
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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metabolism
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Immunohistochemistry
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Lymphatic Metastasis
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Microvessels
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chemistry
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pathology
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Middle Aged
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Stomach
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chemistry
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pathology
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Stomach Neoplasms
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metabolism
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pathology
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Tissue Array Analysis
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methods
6.Evaluation of alveolar bone defect in chronic periodontitis by cone-beam computed tomography.
Hai-jiao ZHAO ; Hong-yan WANG ; Ya-ping PAN
Chinese Journal of Stomatology 2013;48(1):7-11
OBJECTIVETo evaluate the morphological characteristics of alveolar bone defects of the patients with chronic periodontitis using cone-beam CT (CBCT).
METHODSSixty patients with chronic periodontitis were included in this study. CBCT was used to scan the alveolar bone and NNT software to measure the alveolar bone defects and bone loss types in different regions.
RESULTSSeventy-five percent (45/60) of the alveolar bone defect was the generalized type, 25% (15/60) was the localized type. In incisor and canine area, the defect of the mandibular alveolar bone was more severe than in the same sites of maxilla. There was less bone loss in the premolar area of mandible than in the same site of maxilla. In the mesial and buccal sites of mandibular molars and in the lingual site of maxillary molars, the most severe alveolar bone loss was found.
CONCLUSIONSThe obvious alveolar bone defect areas in chronic periodontitis were the palatal side of maxillary molars and the lingual side of mandibular incisors. CBCT can clearly demonstrate the degree of alveolar bone defects in different regions of chronic periodontitis.
Adult ; Alveolar Bone Loss ; diagnostic imaging ; Chronic Periodontitis ; diagnostic imaging ; Cone-Beam Computed Tomography ; Female ; Humans ; Male ; Middle Aged
7.Absolute bioavailability of ginkgolide compounds in rats.
Hai-hong SI ; Ting GENG ; Xiao-ping SUN ; Jie ZHAO ; Jia XUE
China Journal of Chinese Materia Medica 2015;40(14):2882-2886
To investigate the pharmacokinetic characteristics and absolute bioavailability of ginkgolide A (GA), ginkgolide B (GB) and bilobalide (BB) in rats. In this experiment, a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/ MS) method was established to determine the plasma concentrations of GA, GB and BB in rats after rats were administrated with the three drugs through ig and iv respectively. The main pharmacokinetic parameters and absolute bioavailability of three ginkgolide compounds were obtained by using pharmacokinetic software DAS 2. 0. After the inject of GA, GB and BB, the results showed Cmax at (513.9 ± 116.9), (701.3 ± 76.0), (5,255.6 ± 476.8) µg · L(-1) and AUC0.24h of (960.9 ± 268.5), (779.5 ± 140.6), (7,409.3 ± 1,181.1) µg · h · L(-1), respectively; after the oral administration, the results showed Cmax at (522.9 ± 39.9), (146.8 ± 31.6), (2,711.9 ± 588.9) µg · L(-1) and AUC0-24 h of (1,760.4 ± 300.7), (636.6 ± 180.3), (16,651.4 ± 1,306.5) µg · h · L(-1), respectively. The absolute bioavailability of GA, GB and BB in rats was (61.1 ± 10.4)%, (27.2 ± 7.7)%, (56.2 ± 4.4)%, respectively. The method established in this experiment has a good specificity and sensitivity and so can be used to study the pharmacokinetics and absolute bioavailability of GA, GB and BB in rats.
Animals
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Biological Availability
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Chromatography, High Pressure Liquid
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Cyclopentanes
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pharmacokinetics
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Furans
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pharmacokinetics
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Ginkgolides
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pharmacokinetics
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Lactones
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
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Tandem Mass Spectrometry
8.Meta analysis for effect of lead on male productive function.
Hai-hong XU ; Zhi-ping CHEN ; Yi SHEN ; Xue WU ; Fan HE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):634-636
Humans
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Lead
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toxicity
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Male
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Reproduction
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drug effects
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Sperm Count
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Sperm Motility
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drug effects
9.Effects of ginsenosides on the expression of TrkB mRNA in hippocampal formation of aged rats
Hong LAI ; Hai-Hua ZHAO ; Liang ZENG ; Ji-Ping YANG ; Xin FANG ; Yong-Li LV ;
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To investigate the age-related changes of TrkB mRNA in hippocampal formation of aged rats,and the effects of ginsenosides(GS),and provide reliable experimental evidence for anti-aging.Methods 24 Wistar female rats were randomly divided into 3 groups: Young,aged and GS group(fed with GS from 17 to 27 months).In situ hybridization(ISH) method was applied into qualifying and quantitating the TrkB mRNA expression in hippocampal formation.Results TrkB mRNA expression in aged CA3,CA1 and dentate gyrus decreased by 24.2%,13.4% and 50.6%(P
10.A study on anxiety-depression situation and related factors of 120 puerpera before delivery
Chun-Lian TAO ; Li-Li LUO ; Hai-Cheng LIN ; Lin-Hong LUO ; Li-Ping WU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To investigate the status of anxiety-depression and the relevant factors in puerpera be- fore delivery.Methods The emotion of anxiety and depression in puerpera before delivery was evaluated by self-rat- ing anxiety scale(SAS)and self-rating depression scale(SDS)which had satisfactory validity and reliability.Results The values of anxiety and depression in puerpera were higher than those of general crowd(P