Clinical laboratory biological safety is one of whole society safety. This paper introduced briefly the current situation of clinical medical laboratory biosafty in the hospital. and set forth common biological hazards specifically for whose characteristics. Combining the biosafety administration measures from abroad, the issue of laboratory biological safety administration was considered, and put forward some suggestions according to related law and regulation of national laboratory safety administration in order to strengthen clinical laboratory biosafety administration.

Objective To explore the effect of the combination of Weifu-chun and retinoic acid on the expression of Rb Gene and C -erbB -2 Gene in the patients with gastric precancerous lesions.Methods One hundred and fifteen patients with gastric precancerous lesions were ran-domly divided into the observed group ( n =60 ) and the control group ( n=60 ).the group were all given omeprazole enteric -coated capsules and hydrotalcite tablets , and at the same time the observed group was ad-ministrated with Weifuchun ( 1.436 g every time , tid ) and retinoic acid (10 mg every time,tid).The expressions of Rb gene and C -erbB-2 gene were detected by immunohistochemistry and compared after 12 months treatment.Results The positive rate of Rb gene after treatment in the observed group(87.93%) was significantly higher than that before treatment in the observed group (68.97%) and after treatment in the con-trol group(71.93%)(P<0.05).The positive rate of C-erbB-2 gene after treatment in the observed group ( 12.07%) was significantly lower than that before treatment in the observed group ( 34.48%) and after treatment in the control group(29.82%)(P<0.05).Conclusion We-ifuchun and retinoic acid could medicate the expressions of gene Rb and C-erbB-2.

Objective To explore the clinical effect of Shenqifuzheng injection combined with CAF regmien on expression of soluble interleukin 2 receptor(sIL2R), interleukin 6(IL-6), tumor necrosis factor alpha ( TNF-α) in breast cancer patients.Methods One hundred and sixty patients with mammary cancer were randomly divided into the observed group(n=80) and patients in the control group(n=80).They all ac-cepted the treatment of CAF regmien, and patients in the observed group accepted the treatment of Shenqifuzheng injection as well.The concentra-tion of sIL2R, IL -6 and TNF -αwere detected by enzyme -linked immunosorbent assay before and after treatment.Results After treat-ment, the effective rate in the observed group(55.00%) was significant-ly higher than that in the control group(38.75%)(P<0.05);the con-centration of sIL2R, IL-6 and TNF-αafter treatment were significantly lower than those before treatment in the both of the two groups (P<0.01);the concentration of sIL2R, IL-6 and TNF-αin the ob-served group was significantly lower than those in the control group ( P<0.01);the incidence of adverse reactions like blood cells reduce in the observed group ( 35.00%) was significantly lower than that in the control group(52.50%)(P<0.05).Conclusion shenqifuzheng injec-tion could reduce the concentration of sIL2R, IL -6 and TNF -αin breast cancer patients, improve the clinical efficacy, and reduce the adverse reactions.

OBJECTIVETo investigate the clinical symptoms and potential mutation in FGFR3 gene for a family featuring hereditary dwarfism in order to attain diagnosis and provide prenatal diagnosis.

METHODSFive patients and two unaffected relatives from the family, in addition with 100 healthy controls, were recruited. Genome DNA was extracted. Exons 10 and 13 of the FGFR3 gene were amplified using polymerase chain reaction (PCR). PCR products were sequenced in both directions.

RESULTSAll patients had similar features including short stature, short limbs, lumbar hyperlordosis but normal craniofacial features. A heterozygous mutation G1620T (N540K) was identified in the cDNA from all patients but not in the unaffected relatives and 100 control subjects. A heterozygous G380R mutation was excluded.

CONCLUSIONThe hereditary dwarfism featured by this family has been caused by hypochondroplasia (HCH) due to a N540K mutation in the FGFR3 gene.

Base Sequence ; DNA Mutational Analysis ; Dwarfism ; genetics ; Exons ; Female ; Heterozygote ; Humans ; Male ; Mutation ; Receptor, Fibroblast Growth Factor, Type 3 ; genetics

AIM:To evaluate the clinical value of retinal nerve fiber layer (RNFL) thickness, optic disc parameters and macular thickness by optical coherence tomography (OCT) in early diagnosis of primary open angle glaucoma (POAG). METHODS: Sixty-five patients (eighty-five eyes) were selected as a study group and fifty-two normal people (seventy-eight eyes) were selected as a control group. The retinal nerve fiber layer thickness, optic disc parameters, including cup/disc(C/D) rate, cup area (CA), cup volume (CV), disc area (DA), rim area (RA) and rim volume(RV),and macular thickness were detected by Cirrus HD-OCT. The data were compared and analyzed. RESULTS: The retinal nerve fiber layer thickness of superior, inferior, temple, nasal and average in the study group were all significantly thinner than those of the control group (P<0.001). The cup area, cup volume and cup/disc ratio in the study group were significantly higher than those of the control group (P<0.001); while the rim area and rim volume in the study group were significantly lower than those of the control group (P<0 001). But the disc area was not significantly different between two groups(P>0.05). The macular thickness of superior, inferior, temple and nasal in the study group were all significantly thinner than those of the control group (P<0.001). CONCLUSION: OCT can detect the change of retinal nerve fiber layer thickness, optic disc parameters and macular thickness, it is helpful for early diagnosis of POAG.

OBJECTIVETo study the characteristics of genotype spectrum and hematologic parameters in children with HbH disease in the North Guangxi region.

METHODSHbH disease was identified by clinical manifestations, routine blood tests and hemoglobin electrophoresis in 166 children who came form the North Guangxi region. Genotypes were determined by Multi-PCR combined with PCR reverse dot blot. DNA sequencing was used when the genotype could not be identified by regular methods.

RESULTSOf the 166 children with HbH disease, 8 genotypes were identified: --SEA/-α3.7 (82 cases), --SEA/-α4.2 (40 cases), --SEA/αCSα (38 cases), --SEA/αQSα (1 case), --SEA/αWSα (1 case), --SEA/αCD43/44 (-C) α (1 case), --SEA/-α3.7 plus CD17 (A→T) (1 case) and --SEA/-α4.2 plus CD41-42(-TTCT) (1 case). One case was confirmed as the heterozygote of --SEA and an unknown mutation. In the 134 cases with complete medical data, 2 had normal hemoglobin levels, 36 manifested mild anemia, 90 manifested moderate anemia, and 6 (genotype: --SEA/αCSα) showed severe anemia because of the coexistence of infection. Children with the genotype of --SEA/-α3.7 (69 cases), --SEA/-α4.2 (31 cases) and --SEA/αCSα (34 cases) had hemoglobin levels of 62-120, 69-127 and 34-110 g/L respectively. The hemoglobin level in the --SEA/αCSα group was significantly lower than in the deletional HbH disease group (genotypes: --SEA/-α3.7 and --SEA/-α4.2 ) (P<0.05). In contrast, MCV levels in the --SEA/αCSα group were significantly higher than in the deletional HbH disease group (P<0.05).

CONCLUSIONSThe genotype spectrum of HbH disease is diverse in the North Guangxi region. Deletional genotype is prevalent. The disease is heterogeneous. The children with --SEA/αCSα HbH disease have severer anemia and higher MCV levels than those with deletional HbH disease.

Adolescent ; Child ; Child, Preschool ; China ; Female ; Genetics, Population ; Genotype ; Hemoglobin H ; genetics ; Humans ; Infant ; Male ; Mutation ; alpha-Thalassemia ; blood ; genetics

OBJECTIVETo study the protective effect of Sanhuangyinchi Fang drug serum (SF) against hydrogen peroxide-mediated DNA oxidative damage in LO2 cells.

METHODSThe LO2 cells were randomly divided into the control group, H(2)O(2) group, SF groups (5%, 10%, and 15%) and vitE group. The morphological features of the treated LO2 cells were observed under inverted microscope. The viability of the treated cells was assessed with CCK-8 method, and the activity of SOD, CAT and GSH-PX were detected biochemically. Reactive oxygen species (ROS) levels, the content of 8-OHdG, and DNA damage of the cells were evaluated by flow cytometry, ELISA, and Comet assay, respectively.

RESULTSCompared with H(2)O(2) group, the cells in SF groups (10% and 15%) and vitE group showed higher cell survival rate (P<0.05) and higher SOD, CAT, GSH-PX (P<0.05) and ROS scavenging activities (P<0.01) with markedly decreases the content of 8-OHdG (P<0.01) and reduced tailing ratio, tail length, tail moment and Olive tail moment (P<0.05).

CONCLUSIONSF drug serum, especially at the concentration of 15%, can protect LO2 cells from H(2)O(2)-mediated DNA oxidative damage.

Cell Line ; Comet Assay ; DNA Damage ; Deoxyguanosine ; analogs & derivatives ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Hydrogen Peroxide ; toxicity ; Oxidation-Reduction ; Oxidative Stress ; Protective Agents ; pharmacology ; Reactive Oxygen Species

OBJECTIVETo explore the diagnostic value of the dual-energy technique with dual-source computed tomography (DSCT) for anterior cruciate ligament injuries.

METHODSThe clinical data of 8 patients with arthroscopic results were retrospectively reviewed. All patients underwent two- and three-dimensional imaging by multiplanar reconstruction, volume rendering, and tendon mode on DSCT. Dual-energy characteristics were compared with arthroscopic results.

RESULTSSix patients who were arthroscopically diagnosed as anterior cruciate ligament injuries, all of them were also correctly diagnosed by DSCT. Two patients who were arthroscopically diagnosed as normal, one was also diagnosed as normal by DSCT and the other was misdiagnosed. The overall agreement rate was 87.5% (7/8) . Under the dual energy tendon mode, the dual energy staining of the injured anterior cruciate ligament was lower than that of the contralateral normal cruciate ligament of the patient.

CONCLUSIONThe staining diminution in DSCT imaging may be a new feature that can be used to effectively diagnose anterior cruciate ligament injury.

Adolescent ; Adult ; Anterior Cruciate Ligament Injuries ; Female ; Humans ; Knee Injuries ; diagnostic imaging ; Male ; Tomography, X-Ray Computed ; methods ; Young Adult

OBJECTIVESTo analysis the correlation between parameters of sperm chromatin structure assay(SCSA) and semen routine analysis, and to discuss the reliable methods of semen quality evaluation.

METHODSFive hundred and eleven semen samples were detected to analyse the mutiple-parameter correlation between results of SCSA (COMP alpha t) and semen routine analysis.

RESULTSThe parameters that have low-level positive correlation(r: 0.10-0.30) with denatured sperm percentage(COMP alpha t) were viscosity, ejaculation interval, abnormal sperm ratio, concentration of grade c sperm; those having low-level negative correlation(r: -0.30(-)-0.10) were VDL, VSL and VAP; those having mid-level positive correlation (r: 0.30-0.70) were sperm concentration, percentage of grade d sperm; those having mid-level negative correlation (r: -0.70(-)-0.30) were MAD, percentage of grade a sperm and survival rate.

CONCLUSIONSFlow cytometry can be used to evaluate the percentage of denatured or injured sperm rapidly, correctly and simply. The result (COMP alpha t) correlates partly with semen parameters, and it is not a conclusively parameter compared with routine semen analysis. It is important to use SCSA to evaluate productivity under the above situation.

Adult ; Chromatin ; chemistry ; DNA Damage ; Flow Cytometry ; Humans ; Hydrogen-Ion Concentration ; Male ; Semen ; Sperm Count ; Spermatozoa ; ultrastructure ; Viscosity

OBJECTIVESTo detect the sperm DNA damage and to evaluate its significance in male reproductive using single cell gel electrophoresis (SCGE).

METHODSFour hundred and eighteen sperm samples were analysed using the computer assisted analysis system and SCGE. The sperms samples were divided into five grades according to the extent of the sperm nuclear DNA damage.

RESULTS1. When the sperm density is less than 20 x 10(6)/ml, the occurence of grade II and III are increased significantly; 2. In the unmotile grade d sperm the occurence of grade I comet amounts was 5.39%, the occurence of grade II and III was remarkably increased. There was a evidently variance between the grade d and grade a + b sperm.

CONCLUSIONSSCGE can be used to detect the sperm DNA breakage and to evaluate the sperm quality and damage.

Adolescent ; Adult ; Comet Assay ; methods ; DNA Damage ; Humans ; Male ; Sensitivity and Specificity ; Sperm Count ; Sperm Motility ; genetics ; Spermatozoa ; cytology ; metabolism