1.Epidemilogical investigation of pneumoconiosis characteristics in Ningxia from 2002 to 2006.
Qi WANG ; Fu-Hai MA ; Guan-Mei WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):742-743
Adult
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Aged
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Aged, 80 and over
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China
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epidemiology
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Humans
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Middle Aged
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Pneumoconiosis
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epidemiology
2.Function and pathology of the retinal pigment epithelium
Hong-Mei, MA ; Feng-Hai, SUN ; Jian, JIA
International Eye Science 2015;(7):1184-1186
Retinal pigment epithelium ( RPE ) is a vital tissue for the maintenance of photoreceptor function and environment of outer retina, also the predominant cell type of proliferative vitreoretinopathy ( PVR ) . lt is also affected by many diseases of the retina and choroid, including diabetic retinopathy ( DR) , age-related macular degeneration ( AMD ) and retinal pigmentosa ( RP ) . Although of neural origin, the RPE is capable of local repair, and cells may migrate and take on altered characteristics. These findings indicate that RPE-based therapy is a promising option for congenital or degenerate disease of the retina.
3.Study on intra-retinal layers imaged with ultra - high resolution optical coherence tomography
Su-Zhong, XU ; Sheng-Hai, HUANG ; Qing-Kai, MA ; De-Xi, ZHU ; Mei-Xiao, SHEN
International Eye Science 2014;(8):1494-1497
To evaluate the automated segmentation algorithm for detection of intra - retinal layers to process images obtained from ultra- high resolution optical coherence tomography ( OCT ) . Graph theory and the shortest path search based on dynamic programming were applied to automatically segment the 8 intra - retinal layers. We experimentally verified the accuracy and reliability of the algorithm. The results showed that the intra-retinal layer boundaries between automated and manual segmentations matched well. The algorithm successfully segmented the intra- retinal layers in glaucoma, high myopia, and retinitis pigmentosa patients. The proposed automatic segmentation for intra-retinal layers provides a promising tool for quantitative analysis in clinical diagnosis and treatment.
4.Correlations between antioxidant activities and contents of total flavonoids and total phenols of four extracts from Nervilia fordii
Hai-Tang XU ; Shao-Mei MA ; Jin-Yan ZHANG
Chinese Traditional Patent Medicine 2017;39(1):121-125
AIM To investigate the correlations between antioxidant activities and contents of total flavonoids and total phenols of chloroform,ethyl acetate,n-butanol and ethanol extracts from Nervilia fordii (Hance) Schltr..METHODS The contents of total flavonoids and total phenols in four extracts from N.fordii were determined by AlCl3-HAc-NaAc coloration method and Folin-Ciocalteu colorimetric method,respectively.The antioxidant activities of extracts were evaluated by determining scavenging activities of DPPH radical,hydroxyl radical and ABTS radical,together with reducing power.Then the correlations between antioxidant activities and contents of two constituents were analyzed by SPSS17.0 software.RESULTS The antioxidant activities of four extracts from N.fordii all showed dose-effect relationships,that of ethyl acetate extract was the strongest,while that of ethanol extract was the weakest.They had some correlations with the content of total flavonoids (0.604 < r < 0.638) and significant correlations with the content of total phenols (r > 0.977).CONCLUSION Ethyl acetate extract from N.fordii can be used for the development of natural antioxidants,and the content of total phenols is the main factor to influence its antioxidant activity.
5.Two new sesquiterpene lactones from the pericarp of Illicium macranthum.
Hai-juan MA ; Chang-hua MA ; Jian-mei HUANG
Acta Pharmaceutica Sinica 2010;45(3):330-333
Silica gel column chromatography was used for the isolation and purification of the chemical constituents of the pericarp of Illicium macranthum. From dichloromethane-EtOAc (1:1) fraction and EtOAc fraction of the methanol extracts, eleven compounds were identified on the basis of chemical and spectral data. Two new compounds were elucidated to be 6-deoxyneomajucin (1) and 2-oxo-6-deoxyneomajucin (2), along with nine known compounds 6-deoxypseudoanisatin (3), pseudoanisatin (4), anisatin (5), pseudomajucin (6), protocatecheuic acid (7), shikimic acid (8), shikimic acid methylester (9), beta-sitosterol (10) and daucosterol (11). Compounds 1 and 2 are new majucin-type sesquiterpene lactones.
Drugs, Chinese Herbal
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chemistry
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Fruit
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chemistry
;
Illicium
;
chemistry
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Lactones
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chemistry
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isolation & purification
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plants, Medicinal
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chemistry
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Sesquiterpenes
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chemistry
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isolation & purification
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Shikimic Acid
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chemistry
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isolation & purification
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Sitosterols
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chemistry
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isolation & purification
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Spiro Compounds
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chemistry
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isolation & purification
7.Dynamic changes of the epididymal size after vasectomy: An ultrasonographic observation.
Xiao-hai TAO ; Rui-hua LIU ; Bin-gui SUN ; Yi-min MA ; Bei-mei HUANG ; Yu-zhi YUAN
National Journal of Andrology 2015;21(8):717-719
OBJECTIVETo investigate the dynamic changes of the epididymal size 1 year after vasectomy.
METHODSFifty male volunteers received vasoligation. Before and at 1, 2, 3, 6, and 12 months after operation, we measured the size and detected the internal echoes of the epididymis using color Doppler ultrasonography.
RESULTSThe bilateral epididymides were both thickened post-operatively in all the 50 cases, with statistically significant differences between the baseline and the 1st month, the 1st and the 2nd month, the 2nd and the 3rd month, or the 3rd and the 6th month after surgery (all P < 0.01), but not between the 6th and the 12th month (P > 0.05).
CONCLUSIONWithin 6 months after vasectomy, the bilateral epididymides manifested a progressive thickening, but basically restored their balance of secretion-absorption after 6 months.
Epididymis ; diagnostic imaging ; pathology ; physiology ; Humans ; Male ; Organ Size ; Postoperative Period ; Time Factors ; Ultrasonography, Doppler, Color ; Vasectomy
8.Protective effect of baicalin against rotenone induced injury on PC12 cells.
Hai-Lie JI ; Li-Guo TONG ; Chong-Zhi BAI ; Mei-Qing SONG ; Nai-Hong CHEN ; Ma-Li FENG
China Journal of Chinese Materia Medica 2014;39(15):2947-2951
OBJECTIVETo explore the protective effect of baicalin against rotenone-induced injury on PC12 cells, and the po-tential mechanism of action action was also explored.
METHODPC12 cells were injured by rotenone and were treated with different concentrations (0.1, 1, 10 μmol x L(-1)) of baicalin at the same time. Cell viability was analyzed by MTT, and morphology was observed by phase-contrast microscopy. The cell apoptosis was detected by flow cytometry by Annexin V-FITC/PI staining. The intracellular ROS level was determined by fluorescence microscope with DCF-DA staining. The expression of Bcl-2, Bax and Caspase-3 was analyzed by Western blot.
RESULTThe viability of PC12 cells exposure to rotenone for 24 hour was gradually decreased with dose escalating and 1.5 μmol x L was adopted to do the following experiment. Baicalin increased cell viability, improved cell morphology and decreased intracellular ROS level. Moreover, FACS indicated baicalin attenuated the apoptosis induced by rotenone significantly. Western blot showed that Bcl-2, Bax and Caspase-3 expression in rotenone-induced PC12 cells was reversed by baicalin.
CONCLUSIONThis study has demonstrated that baicalin protects PC12 cells against rotenone-induced apoptosis, at least in part, by scavenging excessive ROS and inhibiting the mitochondrion-dependent apoptotic pathway.
Animals ; Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Cell Survival ; drug effects ; Cytoprotection ; drug effects ; Flavonoids ; pharmacology ; Gene Expression Regulation ; drug effects ; Intracellular Space ; drug effects ; metabolism ; PC12 Cells ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Rats ; Reactive Oxygen Species ; metabolism ; Rotenone ; pharmacology ; bcl-2-Associated X Protein ; metabolism
9.Inhibition of osthole for resorption of rats femur tissue in vitro.
Jian ZHOU ; Xue-mei REN ; Xiao-ni MA ; Yu-hai GAO ; Li-juan YAN ; Wen-gui SHI ; Ke-ming CHEN
China Journal of Orthopaedics and Traumatology 2015;28(9):832-837
OBJECTIVETo investigate osthole effect on femoral tissue resorption activity of rat in vitro.
METHODSSix SD rats weighted (80 ± 5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group. The femoral tissue was treated with final concentration of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol culture in vitro at 48 hours after cultured. Tartrate-resistant acid phosphatase (StrACP) activity, glucose and Lactic acid content, StrACP, MCSF (Macrophage colony stimulating factor) and CTSK (Cathepsin K) mRNA was detected by Real-Time RT-PCR were detected.
RESULTSConcetration of Alkaline phosphatase activity were 2226 and 2498 in 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol respectively. As compared with control group, the activity of StrACP of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol were inhibited at 6, 9, 12 days (P < 0.05); under treatment of in l x 10(-5) mol/L osthole, the content of Lactic acid were increased and the content of glucose were decreased at 3, 6, 9 days (P < 0.05); StrACP, MCSF and CTSK mRNA expression level were inhibited at 6, 9 days (P < 0.05).
CONCLUSIONOsthole can inhibit bone resorption and raise the level of nutrition metabolism of femurs tissue.
Acid Phosphatase ; metabolism ; Animals ; Bone Resorption ; prevention & control ; Coumarins ; pharmacology ; Estradiol ; pharmacology ; Femur ; drug effects ; Glucose ; analysis ; Lactic Acid ; analysis ; Male ; Rats ; Rats, Sprague-Dawley
10.Development of Crescentic Immunoglobulin A Nephritis and Multiple Autoantibodies in a Patient during Adalimumab Treatment for Rheumatoid Arthritis.
Xia LI ; Jie MA ; Yan ZHAO ; Hai-Yun WANG ; Xue-Mei LI
Chinese Medical Journal 2015;128(18):2555-2556
Adalimumab
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adverse effects
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therapeutic use
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Antirheumatic Agents
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adverse effects
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therapeutic use
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Arthritis, Rheumatoid
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drug therapy
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immunology
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Asian Continental Ancestry Group
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Autoantibodies
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immunology
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Female
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Humans
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Immunoglobulin A
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immunology
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Middle Aged
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Nephritis
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etiology
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immunology