2.Isolation and identification of cancer stem cells from primary human ovarian cancer tissues.
Sheng LIN ; Hai-xia LONG ; Tong XIANG ; Bo ZHU ; Rong-kai XIE
Chinese Journal of Oncology 2011;33(12):896-899
OBJECTIVETo isolate and identify the cancer stem cells from primary human ovarian cancer tissues.
METHODSFresh tumor tissues from five cases of pathologically diagnosed ovarian cancers were taken, minced and then digested with collagenase and hyaluronidase to obtain single cell suspension. The erythrocytes were removed with ACK Lysis buffer. The suspensions were sorted by magnetic activated cell sorting (MACS) using CD133-binding microbeads. Then the sorted CD133(+) cells were verified by flow cytometry. The cells were cultured in serum-free medium supplemented with EGF, bFGF, insulin and BSA, and grew into spheroids. Immunofluorescence, differentiation and tumor formation tests of the cells were performed to characterize the properties of cancer stem cells.
RESULTSThe ovarian cancer stem cells were successfully isolated from primary human ovarian tumors, which formed typical spheroids in serum-free medium and had stronger ability of tumorigenesis. The results of related experiments verified that CD133 positive cells owned the properties of cancer stem cells.
CONCLUSIONSThe ovarian cancer stem cells presenting the characteristics of stemness in vitro and in vivo, have been successfully isolated from primary human ovarian tumor tissues by MACS. The isolated ovarian cancer stem cells could be used in future researches of their biological functions.
AC133 Antigen ; Animals ; Antigens, CD ; metabolism ; Cell Differentiation ; Cell Separation ; methods ; Female ; Flow Cytometry ; methods ; Glycoproteins ; metabolism ; Humans ; Immunomagnetic Separation ; methods ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Neoplasm Transplantation ; Neoplastic Stem Cells ; metabolism ; pathology ; Ovarian Neoplasms ; metabolism ; pathology ; Peptides ; metabolism
3.Percutaneous alcohol embolization combined with percutaneous vertebroplasty in epidural extension of aggressive vertebral hemangiomas
Yan-Chun XIE ; Yang-Yang ZHAO ; An-Wu XUAN ; Zhuo LI ; Hai-Long YU
Journal of Regional Anatomy and Operative Surgery 2017;26(11):849-852
Objective To evaluate the safety and effectiveness of percutaneous alcohol embolization(PAE) combined with percutaneous vertebroplasty(PVP) for aggressive vertebral haemangiomas (AVHs) with epidural extension.Methods The data of 26 consecutive patients underwent PAE combined with PVP for the treatment of AVHs with epidural extension from October 2013 to November 2015 were analyzed retrospectively.The average follow-up time was (12.21 ± 1.34) months.The pain symptom before and after surgery were evaluated with a visual analogue scale (VAS).Results Pre-procedure mean VAS score was (7.23 ± 1.3) and the postoperative VAS score was (3.11 ± 1.9) at last follow-up.Ten patients (38.5%) remained asymptomatic.Eighty-eight percent of the patients with neurosensory disorders had complete regression of these symptoms.Two of the three patients with motor deficit did not show any improvement.No major complication was recorded.Conclusion PAE combined with PVP is a minimally invasive safe and effective therapeutic approach for AVH with epidural extension.This technique appears mainly effective for pain and neurosensory symptoms,but seems less effective for motor deficit relief.
4.Accuracy analysis of screw-setting through second sacral alar-iliac with interoperative cone beam CT scan
Yang-Yang ZHAO ; Yan-Chun XIE ; An-Wu XUAN ; Zhuo LI ; Hai-Long YU
Journal of Regional Anatomy and Operative Surgery 2018;27(2):128-131
Objective To evaluate the accuracy and safety of screw placement position through second sacral alar -iliac(S2AI)with in-teroperative cone beam CT(CBCT)scan.Methods Collected 22 patients who underwent second sacral alar-iliac screw implant surgery in our hospital from June 2015 to June 2017.All the patients had intraoperative CBCT scan and conventional CT scan after operation.Regard the postoperative CT scan imageing as gold standard to assess the accuracy and safety of S 2AI screws position.The S2AI tract parameters were measured with intraoperative CBCT images and postoperative CT images respectively.Results There was no significant difference between the S2AI tract parameters which were measured with intraoperative CBCT images and postoperative CT images(P>0.05).Conclusion The intraoperative CBCT scan could accurately evaluate the position of S 2AI screws,as well as clarify the damage of the inner and outer plate of the iliac bone in the process of fixation.Compared with postoperative conventional CT scan,the intraoperative CBCT scan can potentially re-duce the reoperation rate.
5.Accuracy and safety of pedicle screw placement for spinal deformity under intraoperative CT-assisted
Yang-Yang ZHAO ; Yan-Chun XIE ; Zhuo LI ; Meng ZHANG ; Guo-Li LIU ; Hai-Long YU
Journal of Regional Anatomy and Operative Surgery 2018;27(10):719-722
Objective To evaluate the accuracy and safety of pedicle screw placement for spinal deformity under intraoperative CT-as-sisted.Methods The clinical data of 28 patients with spinal deformity,who underwent intraoperative CT-assisted placement of pedicle screws in our hospital from March 2014 to March 2017,were analyzed retrospectively.Intraoperative CT-assisted placement of pedicle screws was to observe the position of pedicle screws and adjust the screw position.The safety of pedicle screw fixation was assessed by the postoperative complications.Results All 28 patients of scoliosis were successfully performed surgery.A total of 402 pedicle screws were placed in the spines and the placements of 64 screws of them were adjusted according to intraoperative CT findings.Of 402 screws,377 were placed in the pedicles,13 by grade 1 pedicle breach,8 by grade 2 pedicle breach and 4 by grade 3 pedicle breach according to Gertzbein-Robbins classifi-cation of pedicle screw placement accuracy.No complication was directly related to the screws placement.No patient required reoperation due to the misplacement of the pedicle screws.Conclusion Intraoperative cone-beam CT scan can help the surgeon improve the accuracy and safety of pedicle screw placement for spinal deformity with less complication.
6.Feasibility of implementing percutaneous kyphoplasty in field comprehensive surgical shelter
An-Wu XUAN ; Yan-Chun XIE ; Zhuo LI ; Yang-Yang ZHAO ; Hai-Long YU
Chinese Medical Equipment Journal 2018;39(4):39-42,46
Objective To investigate the feasibility to execute percutaneous kyphoplasty(PKP) in the field comprehensive surgical shelter during kinds of natural disasters. Methods Five experimental pigs underwent PKP in a comprehensive surgical shelter during simulated natural disasters, and were observed for operating time, intraoperative blood loss, complications and postoperative survival rate. Results All the 5 pigs had the operations completed successfully with high postoperative performances in extremities motion and survival rate, and the mean operating time was(43±5.7)min and the mean intraoperative blood loss was (42±3.6)ml.Cement penetrated into the intervertebral space of one pig with satisfactory extremities motion,while no complications occurred in the other pigs.Conclusion The comprehensive surgical shelter proves to enable PKP,and thus extends emergency treatment to the frontier during simulated natural disasters.
7.Protective effect of breviscapine on cardiac function in children after cardiopulmonary bypass undergoing open heart surgery.
Wen-xi XIE ; Li-min YUE ; Hai-long SONG
Chinese Journal of Integrated Traditional and Western Medicine 2010;30(3):264-267
OBJECTIVETo assess the condition of myocardial injury after cardiopulmonary bypass (CPB) and the effects of breviscapine (BVC) on cardiac function in children undergoing open heart surgery.
METHODSThirty-six children (ASA II or III, aged 2-65 months) scheduled to receive ventricular septal defect repairing were randomly assigned to three groups, the control group treated with saline, and the BVC treated groups treated respectively with low dose (0.5 mg/kg) and high dose (1.0 mg/kg) BVC, 12 patients in each group. Saline or BVC (in volume of 15 mL) was administered intravenously after induction of anesthesia with micro-pump within 30 min. Blood levels of troponin I (cTn-I ) and malondialdehyde (MDA) were measured at different time points: pre-operation (T0), during aortic unclamping (T1), and 30 min, 1 h, 6 h, 24 h after aortic unclamping (T2, T3, T4, T5). And the time of operation, CPB, aortic unclamping, and the condition of drainage in 24 h after operation as well as the dosages of narcotics (midazolam, propofol and fentanyl) used were recorded.
RESULTSNo significant difference among groups was found in terms of sex ratio, age, body weight, time of aortic unclamping, CPB and operation, as well as the dosages of narcotics used and the volume of post-operation drainage. Compared with baseline (T0), levels of cTn-I at T1, T4 and T5 increased significantly in all three groups (P<0.01), with the peak revealed at T4; cTn-I in the control group were higher than those in the low dose BVC treated group at T1 and T4 (P<0.01), and those in the high dose BVC group at T1, T4, and T5, while it was insignificantly different between the two BVC treated groups. Level of plasmal MDA began to rise in all groups at T1 with the peak revealed at T2, it lowered after then, and reached the baseline at T5; comparison between groups showed that it was lower in the BVC treated groups than in the control group at T1-T4.
CONCLUSIONSDifferent degree of cardiac injury always happens after open heart surgery and CPB, showing high level of cTn- I within 24 h with the peak revealed at 6 h after aortic unclamping. Intravenous perfusion BVC before CPB at the dose of 0.5 or 1 mg/kg could protect the cardiac function to some extent.
Cardiopulmonary Bypass ; adverse effects ; Child ; Child, Preschool ; Dose-Response Relationship, Drug ; Female ; Flavonoids ; administration & dosage ; therapeutic use ; Humans ; Infant ; Male ; Malondialdehyde ; blood ; Postoperative Period ; Troponin I ; blood
8.Advances in the study of the microbial efflux pumps and its inhibitors development.
Quan-Xin LONG ; Pei-Fu ZHOU ; Zong-Hui WU ; Hong-Hai WANG ; Jian-Ping XIE
Acta Pharmaceutica Sinica 2008;43(11):1082-1088
Drug resistant bacteria is an increasingly urgent challenge to public health. Bacteria adaptation and extensive abuse of antibiotics contribute to this dilemma. Active efflux of antibiotics is employed by the bacteria to survive the antibiotic pressure. Efflux pump is one of the hot spots of current drug related studies and ideal targets for the improvement of treatment. The efflux pumps and related mechanisms of action, regulation of expression and methodologies were summarized. Comparative genomics analyses were employed to elucidate the underlying mechanisms of action and evolution of efflux pump as exemplified by the Mycobacterium in our lab, which is a crucial re-emerging threat to global public health. The pathway and state-of-art drug development of efflux pump related drugs are included too.
ATP-Binding Cassette Transporters
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antagonists & inhibitors
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drug effects
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physiology
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Anti-Bacterial Agents
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metabolism
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pharmacology
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Bacteria
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metabolism
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Drug Resistance, Multiple, Bacterial
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drug effects
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genetics
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Ion Pumps
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antagonists & inhibitors
;
drug effects
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physiology
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Membrane Transport Proteins
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drug effects
;
physiology
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Multidrug Resistance-Associated Proteins
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drug effects
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physiology
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Mycobacterium
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metabolism
9.Gastromegaly infiltrated with plasma cells: a new feature of organomegaly in patients with POEMS syndrome.
Wei-lin XIE ; Jian-long GUAN ; Xing-hai HAN ; Da-lie MA ; Zhen-dong JIN
Chinese Medical Journal 2010;123(10):1356-1358
Aged
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Anti-Inflammatory Agents
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therapeutic use
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Cyclophosphamide
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therapeutic use
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Female
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Humans
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Immunosuppressive Agents
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therapeutic use
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Methylprednisolone
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therapeutic use
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POEMS Syndrome
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complications
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diagnosis
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drug therapy
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pathology
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Plasma Cells
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pathology
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gamma-Globulins
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therapeutic use
10.Effects of fenofibrate on the proliferation and apoptosis and nitric oxide synthase expression of cultured human umbilical vein endothelial cells induced by lysophosphatidylcholine.
Guo-ju SUN ; Xiu-mei XIE ; Ying XING ; Wen-hai YAN ; Tian-lun YANG ; Guo-long YU
Journal of Central South University(Medical Sciences) 2006;31(3):373-378
OBJECTIVE:
To investigate the effects of fenofibrate on the proliferation and apoptosis and endothelial nitric oxide synthase (eNOS) mRNA expression of cultured human umbilical vein endothelial cells (HUVECs) induced by lysophosphatidylcholine (LPC).
METHODS:
HUVECs were cultured in vitro. The study was designated to 5 groups according to fenofibrate concentration: control group, LPC group, LPC + low-concentration fenofibrate (10 micromol/L), LPC + middle-concentration fenofibrate (50 micromol/L), and LPC + high-concentration fenofibrate (100 micromol/L). The study was designated to 6 groups according to the intervention time: control group, LPC group, LPC + fenofibrate (50 micromol/L) 6 h, LPC + fenofibrate 12 h, LPC + fenofibrate 24 h, and LPC + fenofibrate 48 h. The proliferation and apoptosis of HUVECs were evaluated by MTT assay, flow cytometry and fluorescence microscopy, respectively. eNOS mRNA were assayed by real time-PCR.
RESULTS:
Compared with the control group, LPC could inhibit the proliferation and induce apoptosis, and downregulate eNOS mRNA expression and decrease NO production of HUVECs. Fenofibrate could increase the proliferation and decrease the apoptosis, and up-regulate eNOS mRNA expression and enhance NO production in HUVECs.
CONCLUSION
Fenofibrate could improve the proliferation and inhibit the apoptosis, and up-regulate eNOS mRNA expression of HUVECs induced by LPC, which may be responsible for fenofibrate to prevent and treat atherosclerosis.
Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Cells, Cultured
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Endothelium, Vascular
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cytology
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Fenofibrate
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pharmacology
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Humans
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Hypolipidemic Agents
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pharmacology
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Lysophosphatidylcholines
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pharmacology
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Nitric Oxide Synthase Type III
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biosynthesis
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genetics
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RNA, Messenger
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biosynthesis
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genetics
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Umbilical Veins
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cytology