1.Investigation on Impacts of Industrial Fluoride-induced Pollution on Environment and Residents' Health
Journal of Environment and Health 1993;0(03):-
ve To understand the impacts of industrial fluoride-induced pollution on environment and human health. Methods The contents of fluorides in environmental media, such as air, soil, vegetables, weeds, branches and leaves in area around a certain chemical factory (polluted area) were monitored. The prevalance of dental fluorosis, the contents of fluorides in hair and nail were investigated among 87 individuals without history of exposure to industrial fluorides, living in polluted area for more than 5 years, and 132 individuals in control area. Results Higher fluoride levels in environmental medias, higher prevalance rates of dental fluorosis, high contents of fluorides in hair and nails of population were found in polluted area compared with those in control area. Conclusion The in-dustrial fluoride-induced pollution from this chemical factory had resulted in the hazardous impacts on environmental quality and human health.
2.The ethanol extract isolated from Weiqi Decoction induces G₂/M arrest and apoptosis in AGS cells.
Hai-lian SHI ; Bao TAN ; Guang JI ; Lan LU ; Jian-qun XIE
Chinese journal of integrative medicine 2014;20(6):430-437
OBJECTIVETo evaluate the effects of the ethanol extract isolated from Weiqi Decoction (WQD-EE) on AGS cell proliferation and apoptosis.
METHODSBy using high-performance liquid chromatography with ultraviolet detectors (HPLC-UV) assay and MTT method, the main compounds in WQD-EE and cell viability were detected. And cell cycle distributions were determined by flow cytometry with propidium iodine (PI) staining while apoptosis was detected by flow cytometry with annexin V/PI double staining. Finally, caspase-3 activities were measured by colorimetric method and protein expression was determined by Western blotting.
RESULTSHPLC analysis showed that naringin (35.92 μg/mg), nobiletin (21.98 μg/mg), neohesperidin (17.98 μg/mg) and tangeretin (0.756 μg/mg) may be the main compounds in WQD-EE. WQD-EE not only inhibited AGS and MCF 7 cell proliferation in a dose-dependent manner, but also blocked cell cycle progression at G2/M stage as well as inducing cell apoptosis at concentrations triggering significant inhibition of proliferation and cell cycle arrest in AGS cells. While at 0.5 mg/mL, WQD-EE significantly increased caspase-3 activity by 2.75 and 7.47 times at 24 h and 48 h, respectively. Moreover, WQD-EE in one hand reduced protein expressions of p53 and cyclin B1, and in other hand enhanced protein expressions of cytochrome c and Bax. Protein levels of Bcl-2, Fas L and Fas were not significantly affected by WQD-EE.
CONCLUSIONSWQD-EE inhibits AGS cell proliferation through G2/M arrest due to down-regulation of cyclin B1 protein expression, and promotes apoptosis by caspase-3 and mitochondria-dependent pathways, but not by p53-dependent pathway.
Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cell Survival ; drug effects ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; Ethanol ; chemistry ; G2 Phase Cell Cycle Checkpoints ; drug effects ; Humans ; M Phase Cell Cycle Checkpoints ; drug effects ; Neoplasm Proteins ; metabolism ; Plant Extracts ; isolation & purification
3.Prognostic biomarkers and targeted therapies for gastric cancer.
Chinese Journal of Gastrointestinal Surgery 2013;16(2):193-196
Gastric cancer patients with the same clinical stage may vary greatly in prognosis, which makes the improvement of current staging-based treatment imperative. The systematic biology approaches are the solution for systematic identification of biomarkers for prognostic evaluation and targeted therapy. These markers will be applied to classify the patients by molecular traits and then to guide the individualized medication. Although HER-2 antibody is currently the only targeted drug in guideline, more drugs are now addressed in early phases of clinical trials and parts of them will be eventually approved.
Biomarkers, Tumor
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Humans
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Prognosis
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Stomach Neoplasms
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diagnosis
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drug therapy
4.Application of matrix assisted laser desorption ionization-time of flight-mass spectrometry in detecting K-ras gene mutation of colorectal cancer.
Jia-di XING ; Lian-hai ZHANG ; Jing-jing LI ; Zi-yu LI ; Xiang-qian SU ; Jia-fu JI
Chinese Journal of Gastrointestinal Surgery 2013;16(1):80-83
OBJECTIVETo investigate the clinical value of matrix assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) in detecting K-ras gene mutation.
METHODSSixty-one paraffin-embeded specimens of colorectal cancer were selected. MALDI-TOF-MS and regular sequencing were used to test the mutation of codon 12 and 13 in K-ras exon 2.
RESULTSOnly 47 specimens could be detected successfully in regular sequencing, while all the specimens were tested successfully in MALDI-TOF-MS. Fourteen specimens had K-ras mutation in regular sequencing (30.0%), while 22 specimens had mutation in MALDI-TOF-MS (36.1%). Six specimens with mutation were found in MALDI-TOF-MS but were wild-type in regular sequencing. Same mutation types from 14 specimens were confirmed by both regular sequencing and MALDI-TOF-MS. MALDI-TOF-MS was able to detect the mutation in 2 specimens that was not identified in regular sequencing.
CONCLUSIONSMALDI-TOF-MS is a feasible approach of K-ras gene mutation testing in colorectal cancer, which is less demanding in terms of specimen quality and is more sensitive as compared to regular sequencing.
Colorectal Neoplasms ; genetics ; Genes, ras ; Humans ; Mutation ; Sensitivity and Specificity ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods
5.Term structure, code formation and application tools of the clinical care classification system
Ying CHEN ; Yu-Jing JI ; Hai-Xia SUN ; Jun-Lian LI
Chinese Journal of Medical Library and Information Science 2018;27(2):75-80
The term structure of the clinical care classification system(CCC) is consisted of nursing model, nursing components, nursing terms and term modifiers. Briefly described in this paper are its code formation, code genera-tion steps and application tools.
6.Comparison on imaging features of central serous chorioretinopathy fundus
Ji-Jin, ZHANG ; Mei-Yan, WANG ; Feng, XU ; Zhi-Zhong, LI ; Wei, ZHU ; Jie-Li, WU ; Hai-Yan, WANG ; Lian-Cai, WANG
International Eye Science 2014;(10):1887-1889
AIM: To explore and analyze the image features, diagnosis and treatment of the central serous chorioretinopathy ( CSCR) fundus.
METHODS:From May 2008 to May 2014, 97 cases of 121 eyes with central serous chorioretinopathy were treated in in our hospital. The imaging features were compared and analyzed through different methods.
RESULTS:Sixty-one cases (61 eyes) were ≤45 years, including 13 case with disease in both eyes, single stove leak accounted for 48. 6%, multifocal leakage (25. 7%), atypical leakage accounted for 25. 7%. Thirty-six cases (47 eyes) were >45 years, 11 cases with disease in both eyes, single focal leakage ( 8. 5%), multifocal leakage (48. 9%), atypical leakage accounted for 42. 6%. FFA results showed acute hairstyle at the beginning of 89 eyes, chronic deferment type 32 eyes. OCT examination showed that the main features were neuroepithelial detachment, as well as the change of the retinal pigment epithelium ( RPE) layer, which was divided into RPE layer detachment 93 eyes, accounting for 76. 9%, rough and RPE little ridges in 28 cases, accounting for 23. 1%. The average thickness of macular center concave on the cortex of microns was 137. 87 ± 19. 21μm, and there was no significant difference conpared with normal ( 137. 32 ±4.98μm) microns (t=0. 30, P>0. 05). The closer leakage area to macular fovea, the worse of eyesight. .
CONCLUSION: Different imaging examination on central serous chorioretinopathy can show different features. For clinical diagnosis and treatment it had different and complementary roles, but were given significant help for diseases treatment.
7.Effect of Peiminine on MEK1/2,ERK1/2 and the Phosphorylatioin in Lung Tissue of Pulmonary Fibrosis in Rats
Hai GUO ; Fu-Zhi JI ; Xiao-Feng ZHAO ; Jie-Ning GONG ; Jing-Lian QU
Journal of Nanjing University of Traditional Chinese Medicine 2016;32(2):170-175
ABSTRACT:OBJECTIVE To investigate the effect of peiminine on MEK1/2,ERK1/2 and the phosphorylatioin of bleomycin-induced pulmonary fibrosis in rats.METHODS Bleomycin of 5 mg/kg was instilled into the rats with a microliter injector to induce the acute lung injury.Sham-operated group and control group were given distilled water of 1 mL/100 g.Dexamethasone group was given equal volume of dexamethasone distilled water solution.Peiminine A and B group were given equal volume of peiminine distilled water solution of 5 mg/kg and 2.5 mg/kg,respectively.After consecutive daily gavage for 28 d,the rats were anesthetized and killed by carotid exsanguination.Lungs were fixed and embedded,and 4 μm sections were prepared. MEK1/2 and ERK1/2 in the lung tissues were detected by immunohistochemistry.The p-MEK1/2 and p-ERK1/2 were detec-ted by Western blot.RESULTS Peiminine significantly reduced the levels of ERK1/2 and MEK1/2 in lung tissues of rats with bleomycin-induced pulmonary fibrosis(P <0.01),and the effects was similar to dexamethasone (P > 0.05).No significant difference was observed between peimine A group and B group(P >0.05).Compared to the model group and dexamethasone group,peiminine significantly reduced the level of p-MEK1/2 and p-ERK1/2 in lung tissue of bleomycin-induced pulmonary fi-brosis rats(P <0.01).Compared to peimine A group,the B group displayed more significant efficacy(P <0.01).CONCLU-SION Peiminine attenuates the level of MEK1,ERK1 / 2 and the phosphorylation in lung tissues of bleomycin-induced pul-monary fibrosis rats,which may be one of the mechanism of anti-fibrosis.
8.Construction of the nucleic vaccine pVVP3L-18HN and its antitumor effect on human laryngeal carcinoma.
Guo-fang GUAN ; Ning-yi JIN ; Zhi-qiang MI ; Xiao LI ; Hai LIAN ; Chun-shun JIN ; Li-li SUN ; Lian-ji WEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(8):566-570
OBJECTIVENucleic vaccine of pVVP3IL-18HN expressing apoptin gene, Newcastle disease virus HN gene and IL-18 gene were constructed to observe the combinative antitumor effect of the above three genes.
METHODSEukaryotic expression plasmid pVVP3IL-18HN was constructed by inserting apoptin gene and fragment comprising fused IL-18HN gene and IRES promoter into the downstream of CMV promoter of vector pVAX1. The expression of inserted gene was identified by RT-PCR, indirect immunofluorescence and Western-blot. The recombinant plasmid was introduced into Hep-2 cells by liposome, then suppression rate of Hep-2 of different time and different quantity was calculated according to MTT results.
RESULTThe recombinant plasmid of pVVP3IL-18HN suppressed Hep-2 successfully and its suppression rate was up to 61.9% with 20 microg/ml, incubation of 72 hours.
CONCLUSIONThe nucleic vaccine constructed pVVP3IL-18HN had antitumor effect on Hep-2. It may can be used to the therapy and research of laryngeal carcinoma.
Cancer Vaccines ; biosynthesis ; Gene Expression ; Genetic Vectors ; HN Protein ; genetics ; Hep G2 Cells ; Humans ; Interleukin-18 ; genetics ; Laryngeal Neoplasms ; immunology ; prevention & control ; Newcastle disease virus ; immunology ; Plasmids ; Transfection ; Vaccines, DNA ; biosynthesis
9.Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells.
Jing DENG ; Guo-Yuan CHEN ; Jia-Jia JI ; Hao TAN ; Song WANG ; Feng-Xian HU ; Lian-Hua CHAI ; Si-Hai LIU ; Wei-Dong LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(12):730-733
OBJECTIVETo study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.
METHODSSertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.
RESULTSSertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).
CONCLUSIONCS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
Animals ; Apoptosis ; drug effects ; Carbon Disulfide ; toxicity ; Cell Line ; Cell Survival ; Fas Ligand Protein ; metabolism ; Male ; Rats ; Sertoli Cells ; drug effects ; metabolism ; Testis ; cytology
10.Effect of acupuncture at different time points on kidney function and oxygen free radical metabolism in rats with simulated weightlessness.
Yan SONG ; Bo JI ; De-sheng WANG ; He ZHANG ; Bai-xiao ZHAO ; Yong-si XU ; Ping ZHANG ; Jia YANG ; Yu-hai HUANG ; Ya-li LIU ; Xiao-xuan REN ; Wen-lian ZHU ; Jun LU
Chinese Acupuncture & Moxibustion 2014;34(11):1106-1110
OBJECTIVETo explore the effects of pre-acupuncture and immediate acupuncture on kidney function and oxygen free radical metabolism in rats with simulated weightlessness.
METHODSTwenty male clean-grade Wister rats were randomly divided into a normal control group, a model group, a pre-acupuncture group and an immediate acupuncture group, 5 rats in each one. The rats in the normal control group did not receive any treatment but free activities for 4 weeks. The rats in the rest groups received 4-week tail suspension to establish the model of simulated weightlessness. One week before the tail suspension, the rats in the pre-acupuncture group were treated with electroacupuncture at "Shenshu" (BL 23), "Pishu" (BL 20) and "Sanyinjiao" (SP 6) for 30 min per treatment, once a day for 7 days. The rats in the immediate acupuncture group received tail suspension and acupuncture at the same time; during the tail suspension, the electroacupuncture was applied at "Shenshu" (BL 23), "Pishu" (BL 20) and "Sanyinjiao" (SP 6) for 30 min per treatment, once every other day for 14 days. The colorimetric method was used to measure the content of blood urea nitrogen (BUN) in serum as well as activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and content of malonaldehyde (MDA) in renal tissue in each group.
RESULTSCompared with the normal control group, the content of BUN in the model group was increased significantly (P<0.01), the activity of SOD and GSH-PX in nephridial tissue was significantly reduced (both P<0.01), and the content of MDA was increased significantly (P<0.05). Compared with the model group, the content of BUN in the pre-acupuncture group and immediate acupuncture group was significantly reduced (P<0.01, P<0.05), the activity of GSH-PX in the pre-acupuncture group was obviously increased (P<0.05) and the content of MDA in the immediate acupuncture group was increased significantly (P<0.01). Compared with the immediate acupuncture group, the content of MDA in the pre-acupuncture group was lower (P<0.01).
CONCLUSIONThe pre-acupuncture and immediate acupuncture both have the capacity to improve the kidney function and anti-oxygen free radical injury in rats with simulated weightlessness, however, the capacity to increase the protection ability of the kidney and eliminate free radical in the pre-acupuncture group is superior to that in the immediate acupuncture group, which is likely to be related with improving antioxidant ability of kidney.
Acupuncture Points ; Acupuncture Therapy ; Animals ; Antioxidants ; metabolism ; Humans ; Kidney ; metabolism ; Kidney Diseases ; metabolism ; physiopathology ; therapy ; Male ; Malondialdehyde ; metabolism ; Rats ; Rats, Wistar ; Reactive Oxygen Species ; metabolism ; Space Flight ; Superoxide Dismutase ; metabolism ; Weightlessness ; adverse effects