The Her-2 proto-oncogene encodes a 185kDa transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. A chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment was constructed. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, two steps purification method was used to attain the antibody’s purity more than 95%. Both the lyophilized pharmaceutical formulations of the antibody were found. The formulations can keep the stability and activity of the antibody for at least one year. These results were the foundation of the chimeric antibody for cancer therapy.

ObjectiveTo observe the effect of Xiaoyao powder combined with prozac and psychological therapy on post-stroke depression (PSD).Methods85 PSD patients were randomly divided into the treatment group (n=43) and control group (n=42). All patients were treated with routine therapy, including prozac and psychological therapy. Patients in the treatment group were also given Xiaoyao powder. Scores of Hamilton Depression Scale (HAMD), modified Barthel index (MBI) and Scandinavian Stroke Scale (SSS) of all patients were evaluated before and after therapy.ResultsAfter treatment, the effective rate and MBI scores were significantly higher and scores of HAMD and SSS were significantly lower for patients of the treatment group compared with those of the control group (P<0.01).ConclusionXiaoyao powder combined with prozac and psychological therapy can improve depression and neural function of PSD patients significantly.

Objective To investigate the clinical application effect of SPIDER limbs positioning device in arthroscopic surgery of beach chair position.Methods From August 2009 to August 2010 in our hospital 18 cases of patients with rotator cuff injury in shoulder were collected and treated with arthroscopic exploration and suturing operation,including 9 patients in the standard beach chair position under surgical operation,9 cases of beach chairs position combine with SPIDER Limb positioning device.Follow up on the preoperative position placement time,operating time,doctors' satisfaction of surgery posture and postoperative complications.Results The preoperative placement position time of standard beach chair with a mean (27 ± 6.7 ) min,beach chair position combined with SPIDER limb positioning device with a mean (31 ±7.9)min,the two groups were no significant differences ( t =- 1.158,P ＞ 0.05 ) ; the operation time of two groups were ( 78.2 ± 12.7 ) min and (66.2 ± 15.4) min,beach chair position combined with SPIDER limb positioning device group compared with the former was short,there was a between the two groups significant difference; doctors' surgery position satisfaction were 77.8％ and 88.9％ respectively,and there was no complications occurred related with posture.Conclusions SPIDER limb positioning device in the beach chair position in shoulder arthroscopy allows surgeon change positions of limbs during the operation in the sterile area.It has high satisfaction with the surgical posture,practicability,advanced and worthy of clinical application.

OBJECTIVETo study the effect of bacillus bifidus supplementation on the immunity in very-low-birth-weight (VLBW) infants.

METHODSFifty VLBW infants who were hospitalized in the neonatal intensive care unit were equally randomized into observed and control groups. The observed group received bacillus bifidus for 14 days after birth in addition to the conventional management, which was applied in the control group. The clinical indicators and relevant immunological parameters in the peripheral blood were observed.

RESULTSThe times required for brine enema was significantly fewer in the observed group than in the control group (P<0.05), while the incidence of diarrhea showed no significant difference (P>0.05). In the observed group, the proportions of CD4(+) T lymphocytes and the CD4(+)/CD8(+) ratio increased, whilst the proportion of CD3(+) and CD8(+) T lymphocytes showed no significant change. The levels of immunoglobulin A in the peripheral blood increased in the observed group, while the levels of immunoglobulin M and immunoglobulin G were not statistically changed in the observed group when compared with the control group.

CONCLUSIONSBacillus bifidus can improve gastrointestinal symptoms of VLBW infants and have a positive effect on their immunity.

Bacillus ; Dietary Supplements ; Humans ; Immunity ; Infant, Newborn ; Infant, Very Low Birth Weight

With the development of molecular biology, genome science becomes an important subject currently. Characterized by high-throughput, high-integration, high-parallelism, miniaturization and automation, it is the integrated study of gene properties on a large scale. Stroke, an important cerebral vascular disease, is one of the threats to human health. The utilization of microarray study for the pathogenesis of stroke, not only reveals the essentials of the disease in the overall level of genes, but also contributes to the detection of therapeutic targets and the development of novel drugs for stroke. Referring to our own work, this discussion focuses on the progress of the mechanisms underlying experimental cerebral ischemia investigation in vivo as well as anti-cerebral ischemia agents by gene chip technology.
Animals ; Brain ; blood supply ; Brain Ischemia ; genetics ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Hypoxia-Inducible Factor 1 ; metabolism ; Interleukin-6 ; metabolism ; Ischemic Preconditioning ; Neovascularization, Physiologic ; drug effects ; Neuroprotective Agents ; pharmacology ; Oligonucleotide Array Sequence Analysis ; methods ; Reperfusion Injury ; genetics ; metabolism ; Stroke ; genetics ; metabolism

This study was aimed to investigate the distribution of CD38 gene 184 locus allele frequency in chronic myeloid leukemia (CML) and its relation with genetic susceptibility of CML. 100 cases of CML were enrolled in patient group; 200 cases of nonhematologic diseases and nontumor diseases were enrolled in control group. The CD38 gene 184 locus polymorphism was detected by PCR-RFLP, the difference of genotypic frequencies in patient and control groups was analyzed by χ(2) test and Fisher exact probability test, the risk of genotype induced leukemia was expressed by odds ratio (OR) and 95% confidence interval (CI). The results showed that the distribution of CD38 gene 184 locus G/G, G/C, C/C genotypes was no significantly different between patients and control groups (p = 0.072). The wild type C/C was used as reference, the distribution of variant G/C genotype frequency in CML group was different statistically from control group (p = 0.032, OR value 0.517, 95%CI 0.283 - 0.947); the C allele frequency was used as reference, the G allele frequency in CML group was higher than that in control group (p = 0.028, OR value 0.597, 95%CI 0.377 - 0.94). It is concluded that the CD38 gene 184 locus G allele may be an protective gene against CML, and reduce the risk of CML relapse.
ADP-ribosyl Cyclase 1 ; genetics ; Adolescent ; Adult ; Aged ; Alleles ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; genetics ; Male ; Membrane Glycoproteins ; genetics ; Middle Aged ; Polymorphism, Single Nucleotide ; Young Adult

OBJECTIVETo provide reliable data on articular cartilage lesions in different age group of patients with symptomatic knees requiring arthroscopy, and to analyze the characteristics of articular cartilage lesions.

METHODSFrom November 2004 to September 2005, 568 patients were under arthroscopy in orthopaedics of Chinese PLA General Hospital. There were 242 males and 326 females, with a mean age of (43.0 +/- 11.2) years (ranged, 12 to 80 years). Surgery information including causes of injury, onset of symptoms, meniscus lesions, cruciate ligament pathology, articular cartilage lesions were collected by a special Data Management software. All the patients were divided into two groups : youth group and middle-aged group. The data of articular cartilage lesions of patients in the two groups were collected.

RESULTSThere were 923 articular cartilage lesions in 389 patients, and 13 lesions were iatrogenic. In youth group, 212 lesions were found in 121 patients, and the main cause of lesions was menisci menisci injury (57/121 cases). In middle-aged group, 711 lesions were found in 268 patients,and the main cause of lesions was osteoarthritis (218/268 cases). The articular cartilage lesions located majorly in the lateral femoral condyle, medial femoral condyle and patellae in patients of the two groups. The major depth of the lesion was grade IV (330 lesions) in middle-aged group and grade III (71 lesions) in youth group,respectively.

CONCLUSIONArticular cartilage lesions are common in patients under arthroscopy, and articular cartilage lesions of different age group of patients are different, so the best treatment method should be chosen regarding to age factors of patients.

Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Arthroscopy ; Cartilage, Articular ; pathology ; Child ; Female ; Humans ; Knee Joint ; pathology ; Male ; Middle Aged

T cell immune receptor with Ig and ITIM domains (TIGIT), a promising new target in cancer immunotherapy, plays a critical role in limiting adaptive and innate immunity against tumors. The extracellular domain of human TIGIT was used to immune BALB/c mice, and a new anti-human TIGIT chimeric antibody (c7D3) was developed. The mice in this study were used in accordance with the international guidelines for the care and use of laboratory animals, and the animal study was approved by the Institutional Animal Ethics Committee of AbMax Biotechnology. The biological activity of c7D3 was studied. The results showed that c7D3 exhibited high affinity for TIGIT and effectively inhibited the interaction between TIGIT and its ligands. Cell-based assays indicated that c7D3 induced strong luciferase signaling in TIGIT/CD155 signaling reporter assay and enhanced cytokine secretion in a T cell stimulation assay. The data showed that c7D3 has high binding affinity and excellent blocking bioactivity, supporting the further advancement for therapeutic application.

OBJECTIVETo investigate the clinicopathological features of primary gastrointestinal non-Hodgkin's lymphomas (PGI-NHL) and their prognostic values.

METHODSThe clinical and pathological data of 216 patients diagnosed as PGI-NHL from Zhejiang Cancer Hospital were analyzed retrospectively. χ² test, log-liner model analysis, COX proportional hazard regression analysis and Life-table survival analysis were used to analyze the survival status of the patients by SAS 8.2 software, and Log-rank test was performed to couple the overall survival rates with different prognostic factors.

RESULTSTotally, the age of onset was 8 to 89 years with the median age of 56.5 years. Male versus female was 1.27∶1(121∶95). The most frequently involved location was stomach (147 cases, 68.1%), followed by ileocecus (25 cases, 11.6%), large intestine (20 cases, 9.3%), small intestine (17 cases, 7.9%) and multiple GI involvement (5 cases, 2.3%). 182 cases were classified as B cell lymphomas, 22 cases as T cell lymphomas, and 12 cases not classified exactly due to insufficient data. The 3-year and 5-year survival rates of the patients were 69.4% and 53.3%, respectively. Univariate analysis revealed that age＞60 years, ECOG≥2, high LDH level, stage Ⅲ-Ⅳ, IPI≥2, T cell type and intestinal involvement were predictors for poor prognosis. IPI≥2, T cell type and intestinal involvement were independent adverse predictors for prognosis by multiple COX proportional hazard regression analysis. Among different treatment groups, cases received chemotherapy combined with local radiotherapy gained the best survival status.

CONCLUSIONB-cell lymphoma was the main pathological type in PGI-NHL; IPI≥2, T-cell type and intestinal involvement are independent adverse predictors for prognosis; chemotherapy combined with local radiotherapy might be the choice of approach for advanced stage and aggressive PGI-HNL.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Female ; Follow-Up Studies ; Gastrointestinal Neoplasms ; drug therapy ; pathology ; Humans ; Lymphoma, Non-Hodgkin ; drug therapy ; pathology ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Young Adult

Objective To discuss the methods of management and maintenance for arthroscopic surgery instruments and equipments.Methods To construct the management system of arthroscopic surgery instruments and equipments,standardize the process and method of cleaning,disinfecting and maintaining the arthroscopic instruments,and to be strict with the training plan of related persons.Results From January 2003 to June 2008,a total of 3 235 cases of arthroscopic surgery were performed,and no one was influenced by the instruments and equipments' breakdown.Conclusions Strict management systems and careful management and maintenance of instrument and equipment decrease the wear and tear of instruments and equipments,thereby ensuring the success of surgery.