1.Protective effect and mechanism of pinacidil on mitochondrial respiratory function in intestinal mucosa of scalded rats
Hai LIN ; Nianyin PENG ; Hong ZHOU ;
Journal of Third Military Medical University 2003;0(10):-
Objective To investigate the protective effect of pinacidil on the intestinal mucosa of scalded rats and the mechanism. Methods A total of 24 healthy SD rats were randomly divided into normal control group, burn group, and pinacidil treated group. Rats in burn group and pinacidil treated group were inflicted with 30% TBSA Ⅲ degree burn and resuscitated intraperitoneally with Ringer's solution immediately after burn. Pinacidil was injected intraperitoneally into rats in pinacidil treated group at the dose of 2 mg/kg. Mitochondrial respiratory function intestinal mucosa and levels of reactive oxygen species (ROS), maleic dialdehyde (MDA), and superoxide dismutase (SOD) in plasma were determined at 6 h after burn. Results Mitochondrial respiratory function control rate (RCR), ST3, and SOD levels in pinacidil treated group were evidently higher than those in the burn group. However, ST4, MDA, and ROS levels in pinacidil treated group were significantly lower than those in the burn group, but ST4 was not significantly different from that in the normal control. Conclusion Pinacidil can attenuate the damage of intestinal mucosa in scalded rats.
2.In-Vitro Inhibitory Effect of Oleum Curcumae Aromaticae on Leukemia Cell Strain
Hong ZHOU ; Hai SUN ; Yi ZHONG
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
[Objective] To evaluate the therapeutic effect of Oleum Curcumae Aromaticae (OCA) on leukemia cell strain HL-60 and K562 by observing its sensitivity in inhibiting HL-60 and K562. [Methods] Subculture of HL-60 and K562 cells was performed on the 96-hole culture plate and OCA 100?L at the final concentration of 400, 200, 100 and 50mg/L respectively was added. The blank control was added with RPMI-1640 culture fluid. After 24, 48 and 72 hours of treatment, MTF 10?L (5mg/mL) was added and the inhibitory rate on the cells was assayed by detecting the absorption value at 570nm wavelength. [Results] The inhibitory effect of OCA on HL-60 and K562 increased with the treatment time. The difference of inhibitory rates 24, 48 and 72 hours after treatment were significant (P
4.TRPV1 channel-mediated thermogenesis is a common mode for the Chinese pungent-hot or pungent-warm herbs to demonstrate their natures.
Feng SUI ; Li DAI ; Qian LI ; Hai-yu ZHOU ; Hong-dan ZHAN ; Hai-ru HUO ; Ting-liang JIANG
Acta Pharmaceutica Sinica 2015;50(7):836-841
To further uncover the scientific significance and molecular mechanism of the Chinese herbs with pungent hot or warm natures, endogenous and exogenous expression systems were established by isolation of dorsal root ganglion (DRG) neurons and transfection of HEK293 cells with TRPV1 channel gene separately. On this basis, the regulation action of capsaicin, one main ingredient from chili pepper, on TRPV1 channel was further explored by using confocal microscope. Besides, the three-sites one-unit technique and method were constructed based on the brown adipose tissue (BAT), anal and tail skin temperatures. Then the effect of capsaicin on mouse energy metabolism was evaluated. Both endogenous and exogenous TRPV1 channel could be activated and this action could be specifically blocked by the TRPV1 channel inhibitor capsazepine. Simultaneously, the mice's core body temperature and BAT temperature fall down and then go up, accompanied by the increase of temperature of the mice's tail skin. Promotion of the energy metabolism by activation of TRPV1 channel might be the common way for the pungent-hot (warm) herbs to demonstrate their natures.
Adipose Tissue, Brown
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drug effects
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physiology
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Animals
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Capsaicin
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analogs & derivatives
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pharmacology
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Energy Metabolism
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Ganglia, Spinal
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cytology
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HEK293 Cells
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Humans
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Mice
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Neurons
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drug effects
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physiology
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Plants, Medicinal
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chemistry
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TRPV Cation Channels
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physiology
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Temperature
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Thermogenesis
5.Blood transfusion for the treatment of poisoning.
Xin-guo ZHANG ; Jin-zhou BI ; Hai-shi WANG ; Hong-jun LI ; Lui-can LIN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):237-237
Adolescent
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Adult
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Blood Transfusion
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Child
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Child, Preschool
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Female
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Humans
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Male
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Middle Aged
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Pesticides
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poisoning
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Poisoning
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therapy
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Treatment Outcome
6.Adult extrarenal Wilms' tumor occurring in ovary: report of a case.
Li LIANG ; Xin-hua ZHOU ; Yong-jian DENG ; Hong-hai ZHANG ; Yan-qing DING
Chinese Journal of Pathology 2008;37(4):284-285
Female
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Humans
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Kidney Neoplasms
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complications
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pathology
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Ovarian Neoplasms
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pathology
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Wilms Tumor
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complications
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pathology
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Young Adult
7.Viewing from the toll-like receptor/nuclear factor-kappaB signaling pathway to explore the immunomodulatory mechanism of Chinese drugs.
Hong-guang ZHOU ; Hai-bin CHEN ; Mian-hua WU
Chinese Journal of Integrated Traditional and Western Medicine 2010;30(8):884-888
Many Chinese drugs (CHD) have showed their significant effects of integral immune-regulation, and lots of researches have conducted in recent years for exploring their mechanism from different levels, like cytological, molecular and genetic levels. In this paper, the relation between immune-regulation of CHD and Toll-like receptors/nuclear factor-kappaB (TLRs/NF-kappaB) signaling pathway was introduced in brief based upon the achievements of previous researches. It was pointed out that the two are closely related, to explore mechanism of CHD in this way is meaningful not only for further deepening the theoretical understanding of CHD's pharmacological immunoregulation, but also be practically facilitate for enhancing therapeutic efficacy of CHD and developing new CHD.
Adjuvants, Immunologic
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pharmacology
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Drugs, Chinese Herbal
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pharmacology
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Humans
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NF-kappa B
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immunology
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Signal Transduction
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drug effects
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Toll-Like Receptors
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immunology
8.The expression of the early growth response gene-1 after focal cerebral ischemia/reperfusion in rats
Guorong BI ; Jianfei NAO ; Lijuan BAI ; Hemin ZHANG ; Hui ZHANG ; Hong HAI ; Huijie ZHOU ; Xiubing FANG
Chinese Journal of Nervous and Mental Diseases 2007;33(11):644-647
Background To investigate the expression of the early growth response gene-1 ( Egr-1 ) mRNA after focal cerebral ischemia / reperfusion in rats.Methods Ten healthy male SD rats weighing 200 ~ 250 g were used to create model of focal cerebral ischemia. The expression of Egr-1 after focal cerebral ischemia/reperfusion in rats was determined using in situ hybridization and RT-PCR.Results (1) The result of the in situ hybridization: A trace amount of Egr-1 mRNA expressed in the neurons and the glial cells in the sham operated group. The expression of Egr-1 mRNA at the ischemic side increased dramatically following ischemia and reached peaks after 4 hours' reperfusion. Egr-1 expression started to subside following 22 hours' reperfusion and further decreased following 166 hours' reperfusion, which was still significantly higher than that in the sham operated group. (2) The result of RT-PCR: The expression of Egr-1 mRNA at the ischemic side was significantly higher than that in the sham operated group at all time points after ischemia/reperfusion in the rats(P <0. 01). Expression of Egr-1 increased 2 h after ischemia and reached the peak 4 h following reperfusion, and then decreased dramatically at 46 h after reperfusion which was still higher than that in the sham operated group (P < 0. 01). As the ischemia/reperfusion period prolonged, the expression of Egr-1 mRNA increased gradually, but still detectable even 166 h following reperfusion. The expression of Egr-1 was significantly higher than that in the sham operated group at all time points (P <0. 01).Conclusions The expression of Egr-1 mRNA increase in the neurons and the glial cells after ischemia/reperfusion, which may have protective effects on ischemic brain tissues.
9.Effect of Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene on antioxidation activities in mice
Ren-Hai LIU ; Shu-Bin GAO ; Jun ZHANG ; Hong XU ; Ke-Fu ZHOU ;
Chinese Journal of Marine Drugs 2000;0(06):-
Objective To study the biological activities of Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene.Methods Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD were administered orally for 20d to mice,then the activities of glutathione peroxidase(GSH-Px),catalase(CAT),superoxide dismutase(SOD) and the content of malondialdehyde(MDA) were determined.Results The activities of GSHPx in serum and the activities of CAT in blood increased obviously;the activity of SOD in serum and liver increased markedly;the content of MDA in serum and liver decreased obviously.Conclusion Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene had obvious antioxidant effect in vivo.
10.Breeding of High Lipolytical Bacteria by Acclimation to Jatropha Oil and Resultant Strain-catalyzed Esterification Reaction
Li-Hong YUAN ; Jing HUANG ; Yu-Ting LU ; Hai-Xia ZHOU ;
Microbiology 2008;0(12):-
A strain, Pseudomonas sp. X-2-45, with high and stable lipolytical activity was screened by continuously subculturing a lipase-producing bacterium P. sp. LP-1 in culture medium containing Jatropha oil as a sole carbon source. Its hydrolytic activity was 29.79 U/mL, which was increased by 288% as compared to that of parent strain. Furthermore, the growth and lipase synthesis of X-2-45, its catalytic ability to hydrolyze vegetable oils, as well as ester synthesis between fatty acids and organic alcohols were studied. Results showed that rates of bacterial growth and lipase synthesis were significantly raised. Bacterial biomass and lipase activity reached the highest level after 30 h of incubation. Moreover, growth stationary period was prolonged and lipase produced exhibited good stability in culture media during incubation period. Hydro- lytic activity of P. sp. X-2-45 lipase toward Jatropha oil was increased by 378% as compared to parent strain, suggesting that acclimation to Jatropha oil was an effective approach for improving substrate selectivity oflipase. Finally, results of ester synthesis catalyzed by P. sp. X-2-45 lipase indicated that this lipase could catalyze esterification reactions between lauric acid and n-butanol, n-octanol, 1-dodecanol or glycerol, palmitic acid or stearic acid and methanol, n-octanol, 1-dodecanol or glycerol, oleic acid and methanol, n-butanol, n-octanol, 1-dodecanol or glycerol.