1.In-Vitro Inhibitory Effect of Oleum Curcumae Aromaticae on Leukemia Cell Strain
Hong ZHOU ; Hai SUN ; Yi ZHONG
Journal of Guangzhou University of Traditional Chinese Medicine 1999;0(02):-
[Objective] To evaluate the therapeutic effect of Oleum Curcumae Aromaticae (OCA) on leukemia cell strain HL-60 and K562 by observing its sensitivity in inhibiting HL-60 and K562. [Methods] Subculture of HL-60 and K562 cells was performed on the 96-hole culture plate and OCA 100?L at the final concentration of 400, 200, 100 and 50mg/L respectively was added. The blank control was added with RPMI-1640 culture fluid. After 24, 48 and 72 hours of treatment, MTF 10?L (5mg/mL) was added and the inhibitory rate on the cells was assayed by detecting the absorption value at 570nm wavelength. [Results] The inhibitory effect of OCA on HL-60 and K562 increased with the treatment time. The difference of inhibitory rates 24, 48 and 72 hours after treatment were significant (P
2.T lymphocytes in pleural effusion.
Chinese Medical Journal 2008;121(7):579-580
3.Data Mining from Microarray Gene Expression Profile
Yuan-Hai YOU ; Jian-Zhong ZHANG ;
China Biotechnology 2006;0(10):-
Microarray technology are being performed more widely than ever before on many areas in lifescience,although the technology is still evolving,the challenge of performing a microarray experiment is no longer in the data generation,but in extracting useful information and utilizing it to get the results with biological meanings.Some methods and tools used for expressional microarray data mining based on previous work were summarized.These methods include gene clustering,GO analysis,regulating pathway analysis,and related algorithm.We hope this can be helpful for those researchers who are implementing expressional microarray for biological analysis.
4.Residency training in emergency medicine: an arduous and constant challenge.
Xue-Zhong YU ; Ji-Hai LIU ; Zhong WANG
Acta Academiae Medicinae Sinicae 2008;30(2):128-130
With increasing demands and recognition, emergency medicine has advanced rapidly in China in recent decades. The education and training of professionals in this field are important for the further development of emergency medicine. With an attempt to investigate the way to strengthen residency training system in emergency medicine, this article reviews its potential development direction, training modes, learning materials, and quality control.
China
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Emergency Medicine
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education
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organization & administration
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Internship and Residency
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methods
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standards
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trends
6.Implementation of performance metrics in clinical trial data management.
Ya-zhong DENG ; Hong-wei WANG ; Hai-jun FU
Acta Pharmaceutica Sinica 2015;50(11):1488-1492
There is no a systemic performance metrics for clinical data management. While the CDMC in China starts to develop the quality metrics for clinical data management, it is essential to think over the performance and pursue metrics implementation of clinical data management in China. This article provides the basic concept, development and implementation of the performance metric in clinical data management.
China
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Clinical Trials as Topic
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standards
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Data Collection
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standards
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Information Storage and Retrieval
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standards
8.Effect of flunarizine on the epileptiform discharges in cortex and hippocampus of rat with penicillin induced epilepsy.
Guo-feng WU ; Shi-hai SHAN ; You-zhong DONG
Chinese Journal of Applied Physiology 2004;20(2):179-204
Animals
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Cerebral Cortex
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drug effects
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physiology
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Epilepsy
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chemically induced
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physiopathology
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Female
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Flunarizine
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pharmacology
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Hippocampus
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drug effects
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physiology
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Male
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Penicillins
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adverse effects
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Rats
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Rats, Wistar
9.Study on the Quality Standard of Fructus arctii Concentrated Granules
Lanmin ZHEN ; Junshan LI ; Hai JIANG ; Zhong CHEN ; Zhenjiang LI
China Pharmacy 2015;(18):2532-2534
OBJECTIVE:To establish the quality standard of Fructus arctii concentrated granules. METHODS:TLC was con-ducted for the qualitative identification and HPLC was used for the content determination of arctiin in F. arctii concentrated gran-ules. The determination was performed on Dionex C18 column with mobile phase consisted of methanol-water(40∶60,V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm,and the column temperature was 30 ℃. The samples size was 10 μl. RESULTS:TLC spectrum showed that the spots of F. arctii concentrated granules and arctiin reference medicinal material had the same color;the linear range of arctiin was 0.5-12.5 μg(r=0.999 8)with the average recovery of 98.41%(RSD=0.81%, n=9). The RSDs of precision,stability,repeatability tests were no more than 1.0%. CONCLUSIONS:The method is feasible and reproducible,and can effectively control the quality of F. arctii concentrated granules.
10.Clinical Observation on Yishen Paizhuo Decoction for Treatment of Chronic Renal Failure Patients of 42 Cases
Yu-Zhong WANG ; Hai-Cheng WANG ; Xiu-Xia WANG ;
Journal of Traditional Chinese Medicine 1993;0(05):-
Objective To observe the effect of Yishen Paizhuo Decoction(Decoction for reinforcing the kidney and discharging the turbid)on chronic renal failure(CRF)patients.Methods The 82 CRF patients were randomized into observation group(42 cases) and control group(40 cases).In addition to the routine treatment,the observation group was administered modified Yishen Paizbuo Decoction,and the control group was given coated aldehyde oxystarch.The course of treatment was 6 months for both groups. Changes of clinical symptoms,renal functions,blood fat,and hemoglobin were observed,and the linear regression evaluation and renal survival evaluation were employed to evaluate the development of CRF.Results The improvement of observation group in renal func- tions and blood fat was remarkable with the increased renal survival rate,compared with the control group,the difference was signifi- cant(P