1.Effects of posterior screw and rod fixation on nerve function and vertebral indicators in patients with lumbar vertebral fracture
Chinese Journal of Primary Medicine and Pharmacy 2013;20(15):2292-2294
Objective To observe the effects of posterior screw and rod fixation on nerve function and vertebral indicators in patients with lumbar vertebral fracture.Methods The clinical data of 32 cases with lumbar vertebral fracture were analyzed retrospectively.According to Frankle grading:grade A 1 case ;grade B 7 cases;grade C 12 cases ;grade D 12 cases.All the patients were treated by posterior screw and rod fixation.The nerve function and vertebral indicators were compared.Results Followed-up for 22.3 months,according to Frankle grading:grade A 1 case;grade B 3 cases;grade C 9 cases;grade D 15 cases;grade E 4 cases.The nerve functions were improved after operation.There was a significant difference between before and after treatment (all P ＜ 0.05).The the height of the front edge of the fractured vertebral and cobb angle were improved after operation (all P ＜ 0.01).Conclusion Posterior screw and rod fixation can significantly improve nerve function and vertebral indicators in patients with lumbar vertebral fracture.
2.Comparison of the effect of proximal femoral anatomic locking plate and DHS plate in the treatment of femoral proximal fracture
Chinese Journal of Primary Medicine and Pharmacy 2014;21(1):29-30
Objective To compare the clinical curative effect of proximal femoral anatomic locking plate and DHS plate in treatment of femoral proximal fracture.Methods 86 cases of fracture of the proximal femur were randomly divided into the control group of 40 cases,46 cases in the observation group.The control group used DHS plate for treatment,observation group were treated with anatomical proximal femoral locking plate in the treatment of operation,operation time and the clinical efficacy of two groups were compared,the amount of bleeding; follow-up of 6 months,compared with two groups the quality of life of the patients.Results In the observation group,the excellent and good rate was 86.9%,which was better than 75% in the control group (x2 =4.307,P ＜ 0.05).The observation group operation time,operation bleeding volume were (106.3 ± 29.6) min,(171.6 ± 34.1) ml,were significantly better than the control group(149.7 ± 32.7) min,(329.7 ± 47.2) mL(t =6.459,7.569,all P ＜ 0.01).Two groups of patients with postoperative quality of life than the preoperative improve,observation group than in the control group was more obviously improved(t =4.417,P ＜ 0.05).Conclusion The anatomical proximal femoral locking plate is applied to treat proximal femoral fractures,which not only improves the effect of operation,but also improves quality of life.
4.Construction of recombinant adenoviral vector carrying the LEDGFp52 gene by homologous recombination in bacteria and its expression in vitro
International Eye Science 2006;6(5):979-983
AIM: To construct recombinant adenoviral vector carrying the LEDGFp52 gene by homologous recombination in bacteria and to detect its expression in vitro.METHODS: The LEDGFp52 gene was cloned to adenoviral shuttle plasmid pAdTrack-CMV. Then, the resultant pAdTrack-CMV-LEDGFp52 was cotransfected into BJ5 183 bacteria with the adenoviral backbone plasmid pAdeasy-1. The adenoviral plasmid carrying LEDGFp52 was generated with homologous recombination in bacteria, and the adenoviruses were produced in 293 cells. These 293 cells were then infected with adenoviruses, and the expression of LEDGFp52 was detected by CPE (cytopathic effect) and western blot.RESULTS: The titer of Ad-LEDGFp52 adenoviruses was up to 5×1012 pfu/L after proliferation in 293 cells. LEDGFp52 was expressed efficiently in 293 cells after infection.CONCLUSION: The recombinant adenoviruses vector expressing LEDGFp52 was constructed successfully and can be used in further gene transfection experiments.
5.Construction and identification of the eukaryotic expression vector carrying specific siRNA of LEDGF p52 gene
International Eye Science 2006;6(5):975-978
AIM: To construct and identify LEDGFp52 eukaryotic expression vector for RNA interference.METHODS: Recombinants were designed and established by targeting gene LEDGFp52 and plasmid pGensil-1 based on LEDGFp52 cDNA sequences of Genomes. Two pairs of oligonucleotides were synthesized according to the Tuschl principle and inserted into plasmid pGenSil-I to generate siRNA eukaryotic expression vector. DH5α strains were transformed, plasmids were extracted, and recombinant vectors were identified by the restriction map and the sequence analysis. The cultured cells were transfected by the recombinant plasmid (pGensil-1-RNA. LEDGFp52-1). At 48 hours after transfection, the whole cell protein was extracted, and the protein level was detected using Western blotting with mouse anti-human LEDGFp52 monoclonal antibody.RESULTS: Recombinant plasmids completely concord with the designs by the restriction map and the sequence analysis,the proteinlevel of LEDGFp52 was down regulated at 48hours after transfecting pGensil-1- LEDGFp52-1 expression vector into HeLa cells, the recombinant eukaryotic expression vectors were successfully constructed.CONCLUSION: siRNA recombinant can be successfully constructed by RNAi technique to inhibit the expression of LEDGFp52.
6.Construction of prokaryotic expression vector of rhLEDGFp52 gene、 inducing expression and purification of its protein
International Eye Science 2006;6(4):751-754
AIM: To construct the prokaryotic expression vector of rhLEDGFp52 gene,to obtain the rhLEDGF p52 protein.METHODS: rhLEDGFp52 gene was constructed into a prokaryotic expression vector pET30a (+) by recombinant DNA techniques and was identified by enzymatic digestion and sequence analysis. rhLEDGFp52 protein was induced expression by IPTG in E.coli BL21 (DE3), it was tested by Western blot and was purified by Ni-NTA His. Bind. Resin.RESULTS: We Successfully constructed the prokaryotic expression vector of rhLEDGFp52 gene and obtained its expression in E.coli BL21 (DE3),it was expressed in a soluble form and detected up to 34.63% of the total bacterial protein expressed in E.coli BL21 (DE3).Western blot analysis demonstrated that rhLEDGFp52 protein could spicifically integrate with LEDGF-ab. After purified by Ni-NTA His. Bind. Resin, the ultimate concentration of purified rhLEDGFp52 protein was 520μ g/ml and its purity was 87.93%.CONCLUSIONS: rhLEDGF p52 protein was obtained that provides an experimental basis for the further study of the biological function of rhLEDGFp52 protein.
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