Brain ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression ; Neuropeptides ; biosynthesis ; genetics ; Proto-Oncogene Proteins c-fos ; biosynthesis ; genetics ; Proto-Oncogene Proteins c-jun ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics

The changes of morphology, ATP and malonaldehyde (MDA) contents, xanthine oxidase (XO) activity as well as the glutathione peroxidase (GSH-px) activity of rabbit aortic endothelial cells under hyperoxia (100% O_2) for 0-72 hours were studied. We found that cellular morphological changes including swelling, shape variation after hyperoxia were time-dependent; after a temporarily increasing (24hr)(P

0.05).The IL-6 levels of children with M1 and M5 AML were remarkably higher than those of children with the other subtypes of AML(Pa

Objective To investigate the correlation between asymmetric dimethylarginine (ADMA) and endothelial dysfunction in patients with type 2 diabetic nephropathy.Methods The 81 patients with type 2 diabetic nephropathy were selected.According to the urinary albumin excretion rate (UAER),the patients were divided into micro-albuminuria group (DN1 group,UAER 21-199 mg/24 h,38 cases) and macro-albuminuria group (DN2 group,UAER ≥ 200 mg/24 h,43 cases).The 20 healthy people were defined as control group.Intimal-media thickness and endothelial dysfunction of the radial artery of right forearm were detected by color Doppler ultrasound.The serum level of ADMA was determined by enzyme-linked immunosorbent assay.Results There was no significant difference in radial artery inner diameter intimal-media thickness among the 3 groups (P ＞ 0.05).The Endothelial dependent diastolic function (EDD) and endothelial independent diastolic function (EID) in DN1 group and DN2 group were significantly lower than those in control group [(10.45 ± 2.58)％ and (7.56 ± 2.17)％ vs.(15.72 ± 3.05)％,(15.42 ± 2.71)％ and (15.37 ± 2.92)％ vs.(19.31 ± 3.76)％,P ＜ 0.05],and the EDD in DN2 group was significantly lower than that in DN1 group (P＜ 0.05).The serum ADMA in DN1 group and DN2 group was significantly higher than that in control group [(0.63 ± 0.08) and (0.92 ± 0.12) μ mol/L vs.(0.39 ± 0.05)μmol/L,P ＜0.05],and in DN2 group it was significantly higher than that in DN1 group (P ＜0.05).In patients with type 2 diabetic nephropathy,the serum ADMA and EDD had negative correlation (r =-0.81,P =0.020),but the serum ADMA and C reactive protein had positive correlation (r =0.75,P =0.034).Conclusions The serum level of ADMA is significantly increased in patients with type 2 diabetic nephropathy.There is a close correlation between ADMA and endothelial dysfunction of artery.

Objective:To observe the effect of dexmedetomidine used in ophthalmic surgery anesthesia on the recovery quality in children. Methods:Totally 126 children with ophthalmic surgery were randomly divided into the control group and the observation group with the same induction program. The control group was given propofol with target controlled infusion during the operation, while the observation group was given dexmedetomidine 0. 6μg·kg-1 before the induction, and infused up in 10min, propofol with target controlled infusion was used during the operation, and stopped infusion 10min before the end of the operation. The cough number and degree, the occurrence of agitation and extubation time of the two groups were observed and compared during the anesthesia recovery period. Results:The cough incidence and agitation incidence was 36. 51% and 4. 76% in the observation group, respectively, which were significantly lower than those in the control group (74. 60% and 33. 33%, P<0. 05). The cough degree between the two groups showed significant difference (P<0. 05). The first cough time and the extubation time between the two groups showed no significant difference (P>0. 05). Conclusion:Dexmedetomidine used in ophthalmic surgery anesthesia of children can effectively control cough frequency and degree during the recovery period and reduce the agitation to improve the quality of clinical anesthesia recovery.

To measure the relative affinity and type Specificity of neutralizing monoclonal antibodies against the hexon antigen of adenovirus type 7,a simple ELISA double antibody binding system has been developed.By this method,ralative affinity of nine MAbs was estimated from the antibody concentrations at approximately 50% of plateau binding and were ranked.The theoretical basis for this method was discussed. Hybridomas secreting antibodies of desired affinity can be selected at an early stage after fussion by measuring relative affinity of hybridomas supernatants.The type specificity was judged by the difference between end-point concentration against adeno- virus type 7 and type 3.And the relation between affinity and specificity of MAbs was discussed.

Objective To investigate the relationship between metabolic syndrome(MS) and severity of coronary atherosclerosis. Methods Sixty-four hospitalized patients diagnosed as coronary heart disease were divided into MS group(n=26)and non-MS group(n=38).All the patients underwent 16-row multi-slice CT coronary angiography,and cardiovascular risk factors were evaluated. Results The prevalence of MS increased with the number of stenosed coronary arteries(P

Objective: To establish an HPLC fingerprint analysis method of Zhishi Xiebai Guizhi Decoction (ZXGD) and the content determination method of 10 index components of the decoction, and carry out the relevant evaluation and analysis. Methods: HPLC method was used to establish the fingerprint analysis method of ZXGD and then similarity evaluation was carried out. Ten index components of the decoction were determined, the influence of the change of compatibility of medicinal materials on the content of index components was analyzed. Cluster analysis and other chemometrics methods were used to analyze the relevant data and evaluate the impact and value of quality control related indicators of ZXGD. Results: The similarity of 10 batches of samples ranged from 0.376 to 0.990, and that of five batches was more than 0.9, which indicated that the similarity of 10 batches of samples was quite different. In 10 batches of samples, S1, S2, S3, S5, S6, S8 and S10 were in one group, S4 and S9 were in one group, S7 was in one group. In this study, 30 characteristic peaks were calibrated, and principal components 1-6 was the main factor affecting the quality evaluation of medicinal materials. Among the 30 characteristic peaks, peak 21 (neohesperidin), 26, 29 (honokiol), 3, 23, 17, 30 (magnolol), 5, 24 (coumarin), 28, and 7 were the key components. The results showed that except quercetin, the other nine components had good linear relationship, precision, stability and repeatability in the range of mass concentration. Different compatibilities could increase or inhibit the dissolution of related components in medicinal materials. Conclusion: The HPLC method can be used for the simultaneous determination of ten chemical components in ZXGD. The method is efficient, accurate and reproducible, and can be used for the quality control and evaluation of ZXGD.

Objective: To evaluate the effect of Jinqijiangtang Tablet prepared by new technology on blood glucose and lipids in rats with type 2 diabetes. Methods: Type 2 diabetic rat model was established by high fat diet combined with STZ injection. Metformin was used for a positive drug in the present study. Fasting blood glucose, glycosylated serum protein, serum insulin, and insulin resistance index were determined to evaluate the hypoglycemic effect of Jinqijiangtang Tablet prepared by new technology. And serum triglyceride, cholesterol, high density and low density lipoprotein were observed to evaluate the lipid-lowering effect of Jinqijiangtang Tablet prepared by new technology. Results: Jinqijiangtang Tablet prepared by new technology and metformin both could reduce the fasting blood glucose and glycosylated serum protein, and improve glucose tolerance and insulin resistance. Jinqijiangtang Tablet could reduce the levels of cholesterol and HDL.Conclusion: Jinqijiangtang Tablet prepared by new technology can effectively improve insulin resistance, and it has a lipid-lowering effect.

The usage of Chinese materia medica (CMM) is a key scientific problem on its safety and efficacy in clinical application. The daily administration time is one of the main issues and contents of CMM usage, which is directly related to the clinical efficacy. But, it is hardly to find the research aiming at the rationality of daily administration times of CMM. To achieve new breakthrough in research on daily administration times of CMM, one research presumption is put forward for the first time and it is the evaluation pattern for the rationality of daily administration times of CMM based on pharmacodynamics-pharmacokinetics. The further purpose is to supply innovative and practical research thought and method for scientifically confirming the daily administration times of CMM and enhancing its clinical efficacy.