Atherosclerosis ; immunology ; Dendritic Cells ; immunology ; Humans

Microarray technology are being performed more widely than ever before on many areas in lifescience,although the technology is still evolving,the challenge of performing a microarray experiment is no longer in the data generation,but in extracting useful information and utilizing it to get the results with biological meanings.Some methods and tools used for expressional microarray data mining based on previous work were summarized.These methods include gene clustering,GO analysis,regulating pathway analysis,and related algorithm.We hope this can be helpful for those researchers who are implementing expressional microarray for biological analysis.

Child ; Cystitis ; Eosinophils ; Humans ; Male

Objective To provide a scientific basis for logistic health support by analyzing the relationship between altitudes and rate of staff depletion due to acute mountain sickness (AMS). Methods Rate of staff depletion due to AMS at different altitudes was obtained from relevant literature, and statistical methods including descriptive statistics, curve estimation etc. were applied to analyze the relationship between the altitude and rate of AMS-associated staff depletion. Results The AMS-associated staff depletion was positively correlated with the altitude (Pearson correlation r=0.868, P<0.01). The curve fitting equation of AMS-associated staff depletion rate (P) and the square of the altitude (h) in the range of 3500-5270m altitude was: P=64.542-0.034h+4.90×10-6h2, and the goodness-of-fit test showed a very good curve fit (coefficient of determination R2=0.835, F=35.492, P<0.01). There was a remarkable difference in rate of AMS-associated staff depletion between 3000-4000m and 4000m or higher (t=-3.097, P<0.05), with a mean of 5.74%±3.70%, and 95%CI of 2.64%-8.83% and a mean of 17.53%±10.16% and 95%CI of 9.72%-25.34% respectively. Conclusion AMS-associated staff depletion rate is positively correlated with the altitude. The results of this study provide a scientific basis for predicting AMS-associated staff depletion.

ObjectiveTo survey the social support to students in university.Methods400 college students of grade 2004 from 4 different colleges in Ningbo were tested with the Social Support Rating Scale(SSRS).Results and ConclusionThere were no significant differences among students from Ningbo and students from the other regions.Students from different level colleges had different scores in utilization of support,students from the third level college had the highest scores while students from the forth level had the lowest scores.College students from countries scored higher than students from cities in subjective support,but there were no significant differences in objective support and the utilization of support.Female students had higher scores in subjective support and utilization of support than males while had no significant differences in objective support.Students with different majors had no significant differences in objective support and utilization of support,while concerning subjective support,students from management departments had the highest scores,students from arts departments had the lowest scores.In case of emergency,college students mainly received economic support and mental support from their families,friends and classmates while received less support from various organizations both of colleges and society.

ObjectiveTo investigate the relationship between family cohesion, family adaptability and perceived social support.Methods400 college students of grade 2004 from 4 different colleges of Ningbo were tested with the FACESⅡ-CV and perceived social support scale. Results and ConclusionFamily cohesion and adaptability significantly positively correlated with perceived family support and social support. There was no significant correlation between perceived family support and dissatisfaction with family cohesion, while perceived family support significantly negatively correlated with family adaptability. In family adaptability, dissatisfaction with family cohesion and family adaptability, there were significant differences among students from different level colleges. In perceived family support, there were no significant differences among students from different college. In family cohesion, female students scored higher than male, but showed no significant differences in family adaptability. In perceived family support and social support, female students had higher scores than male were. In family cohesion and perceived family support, fathers' education background but not mothers' had significant main effect. In family cohesion, family adaptability and perceived family and social support, there were no significant differences between students from cities or countries.

Objective: To modify EBP(endotoxin binding peptide), clone and express the mutate of EBP gene and gain purified mEBP.Method: mEBPgene was cloned by PCR site-directed mutagenesis. PinpointXa-3/mEBP expression vector was designed to express human mEBP as a fusion protein in BL21 (DE3) pLysS. Digested engineering bacteria by lysozyme and collected inclusion bodies.Fusion protein was purified by Pinpoint TM Xa purification system and cleaved by factorXa,mEBP was purified by RP-HPLC. Results: Mutations at residues 5 and 18(Gln→Lys) was obtained by PCR site-directed mutagenesis, expressed and purified mEBP successfully.Conclusions: Obtaining of purified mEBP lay a foundation for its biological activity research.

Animals ; Cerebral Cortex ; drug effects ; physiology ; Epilepsy ; chemically induced ; physiopathology ; Female ; Flunarizine ; pharmacology ; Hippocampus ; drug effects ; physiology ; Male ; Penicillins ; adverse effects ; Rats ; Rats, Wistar

ObJective To explore the value of acute physiology and chronic health evaluation Ⅲ(APACHE Ⅲ ) score and the concentration of serum cholinesterase (ChE) in predicting the condition and prognosis of patients with severe acute pancreatitis (SAP) within 24 hours afar hospitalization.Methods Sixty-two SAP patients were enrolled and APACHE Ⅲ score was assessed and the concentration of serum ChE was detected within 24 hours after hospitalization.The correlation between the concentration of serum ChE,APACHE Ⅲ score and the condition and prognosis was analyzed.Results There were 44 survivalcases and 18 dead cases.The APACHE Ⅲ score of the surval patients was significantly lower than that of the dead patients [(52.16 ± 13.76) scores vs.(97.10 ± 15.85) scores] (P＜0.01).The concentration of serum ChE of survival patients was significantly higher than that of the dead patients [ (3685 ± 466) U/L vs.(2109 ± 345) U/L] (P＜ 0.01 ).The higher APACHE Ⅲ score was,the lower the serum ChE concentration was,and the higher the mortality rate was.APACHE Ⅲ score and the concontration of serum ChE both had statistical significances compared with the prognosis in the Logistic regression analysis (P =0.0043,0.0075);APACHE Ⅲ score (95％ CI 1.0306-1.1507),the concentration of serum ChE (95％ CI0.9986-1.0125 ).ROC areas under curve (AUC) of APACHE Ⅲ score,serum ChE concentration with the prognosis were 0.936 and 0.882,respectively.There was no significant difference (P=0.0820).In combined prediction of APACHE Ⅲ score and serum ChE concentration,AUC was 0.952,and its predicting accuracy was higher than either APACHE Ⅲ score or serum ChE concentration (P=0.0016,0.0027).Conclusions APACHE Ⅲ score and the concentration of serum ChE both are significantly correlated with the condition and prognosis of SAP patients.Their combined detection can significantly improve the accuracy of prognosis judgement and provide some clinical guidances for treatment.

ObjectiveTo investigate the mental health of freshmen of university.MethodsSymptom Checklist 90 (SCL-90) was used to self evaluate 7763 freshmen of university in Ningbo.Results and ConclusionThe overall level of the mental health of this group was higher than that of the Chinese module, and the prevalence of psychological symptoms was 13.2%. The average level of mental health of female were inferior to male. The difference between the students from cities or towns and from the countryside were distinct. Students who were satisfied with their majority get noticeably higher average score than the ones who were not satisfied.