162 cases with cholelithiasis received endosurgery for cholelithotomy in which rate of relapsed cholelithotomy was 21% and rate of intrahepatic cholelithiasis was 58.8%. For relapsed cholelithiasis, prepared operation (73.5%) found better than emergency operation. The endoscopy helped the biliary examination and found the residue of stone in the liver. The bilio-intestinal anastomosis type Y (11.8%) indicated for cholelithiasis combined with oddi stenosid, many repeated cholelithotomy and much intrahepatic cholelithiasis.
Cholelithiasis ; surgery

The study was carried out on 62 patients with living related donors’ kidney transplant performed in Vietnam from June 4th, 1992 to May 7th, 2002. The results were promising. In recent years, the kidney transplant in our country was improved (39 cases of kidney transplantation with good results were carrying out in 2000, 2001 and 5 months of 2002). Donor selection (in accordance with the process), operation and post-transplant treatment were the most important factors related graft function and health of patient
Kidney Transplantation ; Kidney

The study included 47 patients who were received kidney transplantation from living relative donors at 4 settings in Viet Nam from 6/1992 to 10/2001. Results: 91.5% of patients were in labour age. 97.9% of patients had to have 20 times of haemodialysis and more blood transfusions before transplantation. The common donors are mothers and blood elder sisters. Rate of left kidney removal is 85.1%. The mortality and morbidity decreased dramatically after transplantation. 75% of kidney transplanted patients remained working and 80% have normalised functions
Kidney Transplantation ; Kidney

The study was divided into 2 phases: In phase 1 (1997), the left lobes were removed and transplanted autogenously to other sites. Phase 2 (1998 - 2000) was performed according to the human liver transplantation model. Results: In phase 1, the left lobes were removed in 6 animals, 4 animals were transplanted autogenously at spleen holes. 3/4 grafts had good outcomes. One out of 4 grafts discoloured with poor circulation. In second phase, 23 animals were removed left lobes, 21/23 grafts met requirements, and 5/23 animals had complications and dead soon postoperation. 18/23 animals remained to be survival postoperation. Among liver-received pigs, 5 animals were dead perioperation. 14/18 grafts had good outcomes. 4/18 grafts discoloured. The average extra survival of liver-received pigs was 14.6 hours
Liver Transplantation ; Hepatectomy ; surgery ; therapeutics

OBJECTIVESTwo assay methods, namely the neutral Comet assay andterminal desoxynucleotidyl transferase (TdT) assay, were carried out for comparison to investigate the capability of using the neutral Comet assay as an alternative for detection of apoptosis.

MATERIALS AND METHODSChinese hamster ovary-K1 (CHO-K1) cells were exposed to gamma-rays with different doses and then the frequencies of apoptotic cells were determined at given points of time using the neutral Comet assay andTdT assay.

RESULTSApoptotic frequency of CHO-K1 cells after gamma-irradiation is dependent on both time after irradiation and radiation dose using either the neutral Comet assay orTdT assay. There are differences between the data obtained using the neutral Comet assay andTdT assay (p<0.01, Student's t-test).

CONCLUSIONSThe neutral Comet assay appears to be an appropriate tool for detection of radiationinduced apoptosis at the early stage of the process. Compared to the other methods such as theTdT assay, the neutral Comet assay is a rapid, simple and economical method for detection of apoptosis.

Introduction: The need for gama \u2013 globulin, especially gama - globulin - anti \u2013 HBs, is huge in Vietnam. A number of patients cannot to afford use them due to the high price as they are imported. Meanwhile, Vietnam has high quality input sources for producing gama \u2013 globulin. \r\n', u'Objectives: To study the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs. \r\n', u'Subjects and method: 168 samples of human plasma from voluntary blood donors, which had been screened with for transfusion transmittable infections (TTLs), were chosen as plasma with rich of anti - HBs. The plasma with anti - HBsAg was precipitated with ethanol 25%, pH 6,9 to gain gama - globulin with rich anti - HBs, which was dried by Dutch Ly - 3 - TTE machine. Activation of anti - HBs gama - globulin was identified by a standard method of the degree of antibody specific for anti - HBsAg kit of BIORAD, \r\n', u'Results: The purity of the gama - globulin achieved was 93%, which was almost equal with the results of some foreign researchers (7.8), the activation of anti - HBs was 1:128 dilution degree. The productivity of gama - globulin gaining from 1 litter plasma was 6.0 gram. This new issue was first demonstrated in Vietnam. \r\n', u'Conclusion: We can domestically produce anti-Bs gama - globulin with high degree of activeness (1:128) from human plasma by the precipitating method with ethanol, pH and low temperature. \r\n', u'
Human plasma ; gama - globulin - anti - HBs ; Precipitation of ethanol ; pH and low temperature

As part of our continued study on the chemical constituents of Willughbeia edulis stems, a new dimeric lignan named edulignan (1) was isolated from its EtOAc-soluble extract. Based on NMR spectroscopic interpretation, the planar structure of 1 has been suggested to have two 2-substituted 4-chromanone subunits with different stereochemical configurations. In addition, the MS/MS analysis of the products obtained by acidcatalyzed hydrolysis of 1 was supportive of its structure. Unfornatually, the new compound 1 did not show α-glucosidase inhibitory activity with an IC 50 value > 250 μM.

Objectives: The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association. Methods: A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions. Results: Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001). Conclusion Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.

Background: Melaleuca essential oil has many good effects, bringing economic efficiency; however, low extraction efficiency is a problem that needs to be overcome. This study aims to enhance the efficiency of extracting Melaleuca essential oil by employing deep eutectic solvents (DESs). Materials and methods: Branches and leaves of Melaleuca cajuputi Powell were pretreated by DESs. Carry out the distillation of essential oils by steam distillation. Determination of the chemical composition of essential oils by chromatography-mass spectrometry (GC-MS). Results: The combination of choline chloride and oxalic acid to create DESs solvent system helps the extraction efficiency of Melaleuca essential oil increase by 160%. In addition, the total content of 1,8-cineol and isocineol in the essential oil extracted with DESs solvent was 34.019%, higher than that of the essential oil extracted with distilled water at 30.749%. Conclusions: The positive results show the potential of using DESs solvents to improve the yield and quality of Melaleuca essential oil

Aims: The objective of the study was to isolate bacteriophages and conduct a comprehensive analysis of their potential against Xanthomonas oryzae pv. oryzae (Xoo) strains in the Mekong Delta, Vietnam. Methodology and results: Twelve Xoo strains were isolated from rice fields located in the Mekong Delta, Vietnam. Among these strains, three strains Xoo L019, L020 and L024, showed the highest disease index of bacterial blight. Four phages specific to Xoo were isolated from soil, water and leaf samples, and their morphologies were determined. In a test against 12 Xoo strains, phage L541, MLA23 or W41 could infect 10 of the 12 Xoo strains, while phage LBH01 could infect 8 of the 12 Xoo strains. The stability of the phages to pH, organic solvents, UV-A and UV-B was also evaluated. Conclusion, significance and impact of study The initial characterization of the phages indicates their potential as biocontrol agents against bacterial blight in rice. The study is one of the very first studies about Xoo phages in rice in Vietnam.