Cardiovascular Diseases ; diagnosis ; therapy ; Cerebrovascular Disorders ; diagnosis ; therapy ; Humans ; Medicine, Chinese Traditional ; methods

0.05).The IL-6 levels of children with M1 and M5 AML were remarkably higher than those of children with the other subtypes of AML(Pa

BACKGROUND: Glutamine (Gln) supplementation is known to decrease oxidative stress and inflammatory response, enhance resistance to infectious pathogens, shorten hospital stay, and decrease medical costs of patients. This study was undertaken to evaluate the relationship between the effect of early parenteral glutamine (Gln) supplement on acute liver injury (ALI) and heat shock protein 70 (HSP-70) expression in critical patients. METHODS: Forty-four patients who had been admitted to the emergency intensive care unit (EICU) of Nanjing First Hospital Affiliated to Nanjing Medical University were randomly divided into a control group (n=22) and a Gln group (n=22). The patients of the two groups received enteral and parenteral nutrition. In addition, parenteral Gln 0.4 g/kg per day was given for 7 days in the Gln group. Serum HSP-70 and Gln were measured at admission and at 7 days after admission. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBiL), serum levels of HSP-70 and Gln, mechanical ventilation (MV) time, ICU stay, peripheral blood of TNF-α, IL-6, CD3, CD4 and CD4/CD8 levels were also measured in the two groups. RESULTS: In the Gln group, the levels of serum HSP-70 and Gln were significantly higher after Gln treatment than those before the treatment (P<0.01). HSP-70 level was positively correlated with the Gln level in the Gln group after administration of parenteral Gln (P<0.01). The levels of serum ALT, AST, TBiL and TNF-α, IL-6 were lower in the Gln group than in the non-Gln group (P<0.01). MV time and ICU stay were significantly different between the two groups (P<0.05). The levels of CD3, CD4 and CD4/CD8 were significantly higher in the Gln group than in the control group after treatment (P<0.05). CONCLUSION: Parenteral Gln significantly increases the level of serum HSP70 in critically ill patients. The enhanced expression of HSP70 is correlated with improved outcomes of Gln-treated patients with acute liver injury.

Adult ; Anthropometry ; Enteral Nutrition ; Female ; Humans ; Liver Cirrhosis ; physiopathology ; therapy ; Liver Function Tests ; Male ; Middle Aged

Objective To investigate the correlation between asymmetric dimethylarginine (ADMA) and endothelial dysfunction in patients with type 2 diabetic nephropathy.Methods The 81 patients with type 2 diabetic nephropathy were selected.According to the urinary albumin excretion rate (UAER),the patients were divided into micro-albuminuria group (DN1 group,UAER 21-199 mg/24 h,38 cases) and macro-albuminuria group (DN2 group,UAER ≥ 200 mg/24 h,43 cases).The 20 healthy people were defined as control group.Intimal-media thickness and endothelial dysfunction of the radial artery of right forearm were detected by color Doppler ultrasound.The serum level of ADMA was determined by enzyme-linked immunosorbent assay.Results There was no significant difference in radial artery inner diameter intimal-media thickness among the 3 groups (P ＞ 0.05).The Endothelial dependent diastolic function (EDD) and endothelial independent diastolic function (EID) in DN1 group and DN2 group were significantly lower than those in control group [(10.45 ± 2.58)％ and (7.56 ± 2.17)％ vs.(15.72 ± 3.05)％,(15.42 ± 2.71)％ and (15.37 ± 2.92)％ vs.(19.31 ± 3.76)％,P ＜ 0.05],and the EDD in DN2 group was significantly lower than that in DN1 group (P＜ 0.05).The serum ADMA in DN1 group and DN2 group was significantly higher than that in control group [(0.63 ± 0.08) and (0.92 ± 0.12) μ mol/L vs.(0.39 ± 0.05)μmol/L,P ＜0.05],and in DN2 group it was significantly higher than that in DN1 group (P ＜0.05).In patients with type 2 diabetic nephropathy,the serum ADMA and EDD had negative correlation (r =-0.81,P =0.020),but the serum ADMA and C reactive protein had positive correlation (r =0.75,P =0.034).Conclusions The serum level of ADMA is significantly increased in patients with type 2 diabetic nephropathy.There is a close correlation between ADMA and endothelial dysfunction of artery.

Objective To observe the clinical characteristics and prognosis of primary nephrotic syndrome (PNS) in the elderly. Methods The clinical and pathological features of 52 elderly patients with PNS and 64 non-elderly patients with PNS were retrospectively analyzed and compared.The elderly patients with PNS received routine treatment:oral corticosteroids prednisone 1mg·kg-1 ·d-1.After 8 weeks maintenance treatment,if symptoms were alleviated,the prednisone dose was gradually reduced to a maintenance dose, then was stopped gradually. If symptoms were not alleviated, the cyclophosphamide or cyclosporine, mycophenolate mofetil was added. Results There were significant differences in the numbers of patients complicated with hypertension, infection,chronic renal insufficiency and hematuria between the elderly group and non-elderly group (P＜0. 05＝.But there were no significant differences in the level of blood albumin, quantitative measurement of 24 hours urinary protein and incidence rate of acute renal insufficiency between the two groups (P＞0.05). The major pathological types of PNS in the elderly were membranous nephropathy (46.2 %),IgA nephropathy (23. 1 %) and focal segmental glomerulosclerosis ( 11.5 %), respectively. And the major pathological types of PNS in non-elderly group were mesangial proliferative glomerulonephritis (32.8%), IgA nephropathy ( 25.0% ) and minimal change nephropathy ( 20. 3 %), respectively.Complete remission after treatment was found in 31 patients(59.6%), partial remission in 18 cases and inefficacy in 3 cases. Conclusions The major clinicopathological type of PNS in the elderly is membranous nephropathy and should be treated using corticosteroids and immunosuppressive agents,with different effects in different pathological types.

AIM To study the effect of ATP sensitive potassium channel(K ATP ) opener pinacidil on the current induced by nicotine in cultured rat superior cervical ganglia. METHOD Whole cell patch clamp technique. RESULTS Pinacidil inhibited nicotine induced current when in higher dose(100 ?mol?L -1 ), but had no effect on the desensitization of nicotinic receptors. K ATP antagonist glibenclamide didn't influence the inhibitory effect of pinacidil on nicotinic current. CONCLUSION Pinacidil had an inhibitory effect on nicotine induced current in rat superior cervical ganglia and this effect wasn't mediated by K ATP .

Nicotinic and muscarinic acetylcholine receptors have the same endogenous ligand ACh and are distributed together in many tissues, so it is reasonable to believe that there must be some interactions existed between them. The functions of muscarinic receptors in the tissues innervated by the parasympathetic cholinergic postfibers, can be modulated by the ganglionic nicotinic receptors through stimulating ACh release. In ganglia, the postsynaptic nicotinic receptor activities can be modulated by the presynaptic muscarinic and nicotinic autoreceptors through regulating ACh release. Moreover, The functions of muscarinic receptors can be changed by nicotinic receptor desensitization or blockade. The two types of receptor act on each other and keep in a varied homeostasis of cholinergic nervous system.

Objective To observe the efficiency and safety of Extecavir on chronic severe hepatitis B.Methods 36 patients with chronic severe hepatitis B were randomly divided into 2 groups. Control group (n=16) was given the conventional therapy. Treatment group (n=20) was treated with Entecavir, 0.5mg daily for four weeks, on the bases of routine treatment. Before and after treatment, hepatic function, prothrombin active (PTA), HBVDNA and efficiency rate of the two groups were detected.Results the results showed that the total efficiency rates of treatment group and control group were 85% and 50% respectively. There was significant difference between the two groups (P＜0.01). After treated for four weeks, the improvement of TBil and PTA effect was observed.Conclusion It can be concluded that Entecavir has good therapeutic efficiency and safety in the treatment of chronic severe hepatitis B.

The relation between the critical radius and the particle size distribution for generalized Ostwald type ripening processes whereby the mass transfer coefficient is modelled by a power law was derived. The critical radius is determined by the growth rate, the mass transfer coefficient and the mass balance, and is independent of whether the limiting stationary growth regime has been obtained.
Computer Simulation ; Crystallization ; methods ; Macromolecular Substances ; analysis ; chemistry ; Models, Chemical ; Models, Molecular ; Molecular Conformation ; Particle Size