Objective To verify whether there is intratumoral heterogeneity of DNA ploidy pattern in hep- atocellular carcinoma.Methods Using paraffin-embedded specimens resected from 29 patients with untreated hepatocellular carcinoma (HCC) and having undergone partial hepatectomy,DNA ploidy was analyzed at different sites of the same HCC by means of flow cytometry.Results There was no case in which diploid and aneuploid HCCs cells coexisted in the same tumor.The DNA ploidy pattern was diploid in 13 and aneuploid in 16 cases.A- mong 16 aneuploid tumors,the DNA indices at different sites were similar in 9,but different aneuploid subclones coexisted in 7 cases.Conclusions It is suggested that aneuploid subpopulation from diploid is rare,but new ane- uploid subclones could evolve from aneuploid HCC due to increased instability of their karyotype.

Objective To observe the clinical effect of treating humeral supracondylar fracture in children with manual reduction combined with oral medicinal herbs and fumigation.Methods 80 children with humeral supracondylar fracture were involved in clinical observation.Patients with Gartland Type Ⅰ and Type Ⅱ were selected and treated with manual reduction and plaster external fixation.Gartland Type Ⅲ cases were selected and treated with Kirschner-wire transfixion.All of patients were treated with oral medicinal herbs and fumigation.Results All children with humeral supracondylar fracture recovered.Based on the clinical function test,39(48.75%)cases had excellent effects,39(48.75%)cases had good effects,and 2(2.50%)cases had fair effects.No Volkmann contracture or cubitus varus deformity occurred.Conclusion The treatment of humeral supracondylar fracture in children with manual reduction combined with oral medicinal herbs and fumigation has a good effect.

ATP sensitive potassium channels (K ATP ) have been thought to be a mediator of cardioprotection. Recent pharmacologic and molecular biology studies show the protective effects of K ATP openers are not dependent of action potential shortening and cardiodepressant effects, but mediated by preservation of mitochondrial function, which suggests a role for intracellular mitochondrial K ATP (mito K ATP ). The mechanism by opening mito K ATP produces cardioprotection is less clearer, however, it is thought that a beneficial effect may occur as the result of K + entry and intramitochondrial depolarization. This effect would reduce mitochondrial calcium overload and cause matrix swelling, which are shown to enhance ATP synthesis and stimulate mitochondrial respiration.

Objective To observe the effect of self mading Tongfengxiao decoction in treatment of acute gouty arthritis. Methods 69 patients were randomly divided into two groups. In the control group,33 patients were given diclofenac sodium sustained-release capsules and allopurinol treatment;In treatment group ,36 patients were given to the self mading Tongfengxiao decoction in addition to the western medicine received by the control group. The two groups were all treated for 14 days. Results The total effective rate was 94.4% for treatment group and 87.8% for control group respectively. Therefore, the difference of treatment effect between twp groups was significant ( P ＜0. 05 ) ;Also, the treatment group performed better than control group in term of reducing serum uric acid( P ＜ 0. 05).Conclusion The treatment of Tongfengxiao decoction combined western medicine for acute gouty arthritis had a good synergistic effect, and could reduce serum uric acid and improve clinical symptoms.

Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Extracellular Matrix Proteins ; biosynthesis ; Humans ; Liver ; metabolism ; pathology ; Liver Cirrhosis ; drug therapy ; metabolism ; pathology ; Medicine, Chinese Traditional ; Phytotherapy

Open ocular trauma in children is one of the main causes of non-congenital monocular blindness in children. Children’s psychological and daily activities are different from those of adults; the eye tissue structure is not maturely enough, and the characteristics of high response to injury and incompatibility with various diagnostic and treatment methods determine the factors that cause injuries and injuries in children with open ocular trauma. The environment, diagnosis and treatment, and prognosis of children are more complicated than adults. For the emergency of first and second stage on diagnosis and treatment of children with open ocular trauma, it should follow the principles of adult open ocular trauma management, combined with the children's characteristics, clarifying the anatomical location of the ocular trauma before surgery, and closing the wound during the first-stage operation. At the same time, the disturbance of intraocular tissue should be minimized, the timing of the second stage operation and the individualization of the operation design, and the medication and care after the operation should also be combined with the characteristics of children. Prevention of ocular trauma in children is the focus of attention. In the new information era, clinical research and public information platforms should be fully utilized to advance the epidemiological study of children's ocular trauma and guide the construction of the prevention and treatment system for children's ocular trauma more scientifically.

Objective To investigate the risk factors that affect the perioperative mortality incholangiocarcinoma.Methods The clinical data of sixty two patients with cholangiocarcinoma who underwent operation were reviewed retrospectively.Logistic regression test was used in the perioperative mortality study.Before multivariate analysis,each parameter was evaluated with univariate analysis to define whether it was of statistical significance(P

Cardiovascular Diseases ; Humans ; Osteoporosis

Objective: To provide anatomic data for accurately locating the important structures of sellar area and preventing injury during endoscopic extended transsphenoidal approach to the sellar area. Methods: The extended transsphenoidal approach was employed in twenty cadavers via endoscope, and the posterior wall of sphenoid sinus was divided into different parts for localizing sella turcica, carotid protuberance and optic canal. Important structures were observed and measured under microscope. Results: The posterior wall of sphenoid sinus was divided into five parts according to the four lines along the two sides of the two carotid protuberances. The depths of the sella turcica in the tuberculum sellae, anterior wall of sella turcica and sellar floor were (2.20±0.19 [0.63-6.90]) mm, (0.71±0.06 [0.36-2.78]) mm, and (0.94±0.18 [0.23-2.81]) mm, respectively. The transverse diameter, sagittal diameter and coronal diameter of pituitary gland were (14.72±1.07 [10.92-18.38]) mm, (10.13±0.92[9.37-12.51]) mm and (5.13±0.74 (2.37-7.91]) mm, respectively. Conclusion: The five parts of the posterior wall of sphenoid sinus in this study can facilitate the localization of important structures in the sellar area during the extended transsphenoidal approach to sellar area via endoscope, so as to prevent iatrogenic injury of vital structures in the sellar area.

Objective: To search for a safe approach for transsphenoidal endoscopic pituitary surgery, so as to avoid injury of intercavernous sinuses during the operation. Methods: The anterior intercavernous sinus, posterior intercavernous sinus, inferior intercavernous sinus, basilar sinus, and dorsum sella sinus of 20 Chinese adult cadavers were surgically observed; and the relevant data were obtained and analyzed. Results: The existence rates of anterior intercavernous sinuses, posterior intercavernous sinuses, inferior intercavernous sinuses, basilar sinuses, dorsum sella sinuses were 95%(19), 75%(15), 10%(2), 100% (20) and 30%(6), respectively; their anteroposterior diameters were (2.08±0.90) mm, (5.14±2.54) mm, (1.30±0.40) mm, (2.26±1.02) mm, and(2.01 ± 0.80) mm, respectively; and their supra-inferior diameters were (2.74 ± 0.96) mm, (1.10±0.74) mm, (1.48±0.29) mm, (15.67±4.54) mm, and (3.35±1.93) mm, respectively. The distance between inferior edge of anterior intercavernous sinus and the anterior edge of posterior intercavernous sinus was (5.78±1.89) mm. Conclusion: The intercavernous sinus should be avoided to expose the pituitary after opening the antapex of sella trucica during transsphenoidal endoscopic pituitary surgery. When the intercavernous sinus can not be avoided, reasonable incision and hemostatic method should be selected before operation to prevent severe bleeding.