1.Development of a universal plate-agglutination test for detecting Haemophilus parasuis.
Dingqian GUO ; Cheng TANG ; Quan HAI ; Guoqing SHAO ; Hua YUE
Journal of Veterinary Science 2010;11(4):355-357
Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
Agglutination Tests/*methods
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Animals
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Cross Reactions
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Haemophilus parasuis/isolation & purification/*physiology
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Immune Sera/*metabolism
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Reproducibility of Results
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Sensitivity and Specificity