1.Evaluation of Early Neurologic Abnormalities on the Workers Exposed to Acrylamide.
Seong Soon KIM ; Hae Kwan CHEONG ; Yong Wook KWON
Korean Journal of Occupational and Environmental Medicine 2000;12(3):367-383
OBJECTIVES: Acrylamide is a neurotoxin inducing central-peripheral distal axonopathy. The authors evaluated several neurologic tests to screen the early effect of occupational acrylamide exposure. METHODS: Fifty-two males working either in the production of acrylamide monomer or in the handling of acrylamide in Ulsan, Korea, were selected and subcategorized into the high-exposure group (n=10, mean age of 30. 4 years) and the low-exposure group (n=42, mean age of 28.9 years). Twenty-three males (mean age 29.4 years) from medical professionals and students were chosen as a non-exposed reference group. Symptom questionnaire, neurologic examination, electrodiagnostic test, vibrotactile threshold test, and Lanthony desaturated 15 panel test (LD-15D) were done. RESULTS: The high-exposure group showed more number of specific symptoms on questionnaire and more abnormal two point discrimination test. High-exposure group showed a significant difference in the median motor conduction velocity, peroneal nerve motor latency, sensory amplitude of median nerve action potential, and sensory latency of sural nerve. The vibrotactile threshold of the high-exposure group was significantly higher in both hands and feet than that of the low-exposure group and the reference group. Average color confusion index on the LD-15D was significantly higher in the high-exposure group (median 22.7, range 0-135.7) than in the reference group (median 2.4, range 0-33.2). CONCLUSIONS: The symptom questionnaire, vibrotactile threshold test, and color discrimination test are sensitive in detection of the early acrylamide-induced neuropathy, and the combination of these tests would further facilitate the effectiveness of the screening.
Acrylamide*
;
Action Potentials
;
Discrimination (Psychology)
;
Foot
;
Hand
;
Humans
;
Korea
;
Male
;
Mass Screening
;
Median Nerve
;
Neurologic Examination
;
Peroneal Nerve
;
Questionnaires
;
Sural Nerve
;
Ulsan
2.Cephalometric analysis of mandibular growth in rabbits.
Hae Wook LEE ; Sung Tack KWON ; Chin Whan KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1991;18(2):216-221
No abstract available.
Rabbits*
3.PUVA Therapy of Pityriasis Lichenoides Chronica.
Hae Ki HAN ; Jin Kwon KIM ; Hong Il KOOK
Korean Journal of Dermatology 1982;20(3):413-417
Pityriasis lichenoides chronica is characterized by unknown etiology, chronicity and by being essentially asymptomatic and refractory to therapy. Nine patients with pityriasis lichenoides chronica were treated with orally administrated 8-Methoxypsoralen and UVA irradiation(PUVA Therapy). After S-45 PUVA treatments, lesions were completely cleared.
Humans
;
Methoxsalen
;
Pityriasis Lichenoides*
;
Pityriasis*
;
PUVA Therapy*
5.Effects of Follicle Cells on the Chymotrypsin Resistance of Mouse Oocytes.
Seong Im KIM ; In Ha BAE ; Hae Kwon KIM ; Sung Rye KIM
Korean Journal of Fertility and Sterility 1999;26(3):407-417
OBJECTIVE: Mammalian follicle cells are the most important somatic cells which help oocytes grow, mature and ovulate and thus are believed to provide oocytes with various functional and structural components. In the present study we have examined whether cumulus or granulosa cells might play a role in establishing the plasma membrane structure of mouse oocytes during meiotic maturation. DESIGN: In particular the differential resistances of mouse oocytes against chymotrypsin treatment were examined following culture with or without cumulus or granulosa cells, or in these cell-conditioned media. RESULTS: When mouse denuded oocytes, freed from their surrounding cumulus cells, were cultured in vitro for 17~18 hr and then treated with 1% chymotrypsin, half of the oocytes underwent degeneration within 37.5 min (t50=37.5+/-7.5 min) after the treatment. In contrast cumulus-enclosed oocytes showed t50=207.0. Similarly, when oocytes were co-cultured with cumulus cells which were not associated with the oocytes but present in the same medium, the t50 of co-cultured oocytes was 177.5+/-13.1 min. Furthermore, when oocytes were cultured in the cumulus cell-conditioned medium, t50 of these oocytes was 190.0+/-10.8 min whereas t50 of the oocytes cultured in M16 alone was 25.5+/-2.9 min. Granulosa cell-conditioned medium also increased the resistance of oocytes against chymotrypsin treatment such that t50 of oocytes cultured in granulosa cell-conditioned medium was 152.5+/-19.0 min while that of oocytes cultured in M16 alone was 70.0+/-8.2 min. To see what molecular components of follicle cell-conditioned medium are involved in the above effects, the granulosa cell-conditioned medium was separated into two factions by using Microcon-10 membrane filter having a 10 kDa cut-off range. When denuded oocytes were cultured in medium containing the retentate, t50 of the oocytes was 70.0+/-10.5 min. In contrast, t50 of the denuded oocytes cultured in medium containing the filtrate was 142.0+/-26.5 min. T50 of denuded oocytes cultured in medium containing both retentate and filtrate was 188.0+/- 13.6 min. However, t50 of denuded oocytes cultured in M16 alone was 70.0 +/-11.0 min and that of oocytes cultured in whole granulosa cell-conditioned medium was 156.0+/-27.9 min. When surface membrane proteins of oocytes were electrophoretically analyzed, no difference was found between the protein profiles of oocytes cultured in M16 alone and of those cultured in the filtrate. CONCLUSIONS: Based upon these results, it is concluded that mouse follicle cells secrete a factor(s) which enhance the resistance of mouse oocytes against a proteolytic enzyme treatment. The factor appears to be a small molecules having a molecular weight less than 10 kDa.
Animals
;
Cell Membrane
;
Chymotrypsin*
;
Cumulus Cells
;
Female
;
Granulosa Cells
;
Membrane Proteins
;
Membranes
;
Mice*
;
Molecular Weight
;
Oocytes*
6.Studies on the lectin binding patterns of the oviduct luminal epithelium and oocytes in mouse.
Myung Chan GYE ; Sung Rye KIM ; Hae Kwon KIM ; Moon Kyoo KIM
Korean Journal of Fertility and Sterility 1993;20(3):267-276
No abstract available.
Animals
;
Epithelium*
;
Mice*
;
Oocytes*
;
Oviducts*
;
Phenobarbital*
7.The experimental study on the effects of Ringer's lactate andpentastarch infusion in hemorrhagic dogs.
Woog Seong KIM ; Jae Young KWON ; Hae Kyu KIM ; Inn Se KIM ; Kyoo Sub JUNG
The Korean Journal of Critical Care Medicine 1992;7(2):105-112
No abstract available.
Animals
;
Dogs*
;
Lactic Acid*
8.Effects of mutation of hepatitis B virus poly(A) signal "TATAAA" to "AATAAA" on expression of hepatitis B viral genes.
Sang Hae KIM ; Seong Kee KIM ; Yong Tae KWON ; Hyune Mo RHO
Journal of the Korean Society of Virology 1992;22(1):37-44
No abstract available.
Genes, Viral*
;
Hepatitis B virus*
;
Hepatitis B*
;
Hepatitis*
9.a case of primary adenocarcinoma of fallopian tube.
Kwon Il ROH ; Hae Suk KIM ; Duk Soo BAE ; Chang Jae SHIN ; Jung Gu KIM
Korean Journal of Obstetrics and Gynecology 1991;34(1):144-151
No abstract available.
Adenocarcinoma*
;
Fallopian Tubes*
;
Female
10.A Case of Congenital Lipoid Adrenal Hyperplasia: Early Diagnosis by Using Computed Tomography.
Jung Hyun KWON ; Ji Young KIM ; Hae Soon KIM ; Sun Wha LEE ; Han Wook YOO
Journal of Korean Society of Pediatric Endocrinology 2004;9(2):208-212
Congenital lipoid adrenal hyperplasia, the most severe form of congenital adrenal hyperplasia, is caused by mutations in the steroidogenic acute regulatory protien (StAR). It is characterized by failure of synthesis of all three classes of adrenal steroids and massive accumulation of lipids and cholesterol in the adrenal cortex. The computed tomography (CT) unequivocally delineated massively enlarged adrenal glands of fat-tissue attenuation, enabling early diagnosis and replacement therapy. We report a case of congenital lipoid adrenal hyperplasia, in which CT established that lipoid deposition at the adrenal cortex disappeared after the adrenal hormone replacement therapy.
Adrenal Cortex
;
Adrenal Glands
;
Adrenal Hyperplasia, Congenital
;
Cholesterol
;
Early Diagnosis*
;
Hormone Replacement Therapy
;
Hyperplasia*
;
Steroids