1.Expression of Ras Oncogene in the Intracpithelial Neoplasia and Carcinoma of the Uterine Cervix.
Sang Su PARK ; Kyu Yun CHOI ; Kae Hyun NAM ; Kwon Hae LEE
Korean Journal of Gynecologic Oncology and Colposcopy 1996;7(3):217-225
Cellular oncogenes are expressed as an intrinsic part of the transformed or neoplastic phenotype. More than 60 of the known cellular oncogenes play a specific role in normal cellular development and differentiation. To examine the correlation between ras oncogene expression and the development of cervical cancer, this study investigated the reactivity of cervical intraepithelial neoplasia(CIN) and carcinoma of the uterine cervix by using anti-ras P21 mouse monoclonal antibody. The expression of ras oncogene significantly increased with the grade of malignancy from 11% in severe dysplasia, 30% in carcinoma in situ, 43% in microinvasive carcinoma, to 53% in invasive cancer. The expression of ras oncogene was not correlated with histologic type, tumor size, and nodal status of cervical cancer. It was concluded that expression of ras oncogene is related to early phase of carcinogenesis and tumor invasion of carcinoma of the uterine cervix.
Animals
;
Carcinogenesis
;
Carcinoma in Situ
;
Cervix Uteri*
;
Female
;
Genes, ras*
;
Mice
;
Oncogenes
;
Phenotype
;
Uterine Cervical Neoplasms
2.Relationship of Body Fat Distribution and Serum Lipids in Men.
Byung Yeol CHUN ; Min Hae YEH ; Sung Kook LEE ; Yun Kyeong RHO ; Soon Yeol NAM
Korean Journal of Epidemiology 1994;16(1):28-40
This study was conducted to investigate the relationship of body fatness indicators and serum lipids (total cholesterol, high density lipoprotein (HDL) cholesterol, low density lipoprotein(LDL) cholesterol, triglycerides, myocardial infarction(MI) index) to find anthropometric indicators of body fat distribution that are strongly associated with serum lipids. 347 adult male workers of an industry in Kyungpook province were selected and data on the general characteristics were obtained using questionnaire from June 26 to June 29, 1991. Serum lipids were tested with 5 ml fasting blood and height, weight, circumferences of waist, hip and mid-thigh, five subcutaneous fat thickeness(biceps, triceps, supscapular, suprailiac, thigh) were mea sured using Ultrasound type A TATT. Waist circumference, waist/hip circumference ratio and subcutaneous fat thickness of suprailiac area are significantly increased with increasing age, but thigh circumference is significantly decreased (P< 0.05). These results suggest that central obesity is closely related to age and waist circumference may be the best indicator of body fatness or body fat distribution in men. In younger age group(below 40 year old), the result of canonical correlations analysis suggests that circumferences(0.501) was slightly closely related to serum lipids than subcutaneous fat thickness(0.493), however, in older age group (above 40 year old), subcutaneous fat thickness(0.528) were more strongly associated with serum lipids than circumferences(0.419). Weighted canonical analyses suggest that biceps and suprailiac areas in younger age group and sub scapular area in older age group are stronger indicators of serum lipids among .subcutaneous fatness measurements. Of the circumferences, waist is the most strongest indicator of serum lipids in both age groups. Therefore, it is recommended that circumference of waist and subcutaneous fat thickness of subscapular area should be measured to estimate the degree of central obesity for preventing cardiovascular disease in men.
Adipose Tissue*
;
Adult
;
Body Fat Distribution*
;
Cardiovascular Diseases
;
Cholesterol
;
Fasting
;
Gyeongsangbuk-do
;
Hip
;
Humans
;
Lipoproteins
;
Male
;
Obesity, Abdominal
;
Subcutaneous Fat
;
Thigh
;
Triglycerides
;
Ultrasonography
;
Waist Circumference
;
Surveys and Questionnaires
3.A Case of Acardiac Twin.
Seong Yun JEONG ; Hae Hyeog LEE ; Seok Min LEE ; Hwan Sung JAE ; Kye Hyun NAM ; Im Soon LEE ; Kwon Hae LEE ; Dong Won KIM
Korean Journal of Perinatology 2000;11(2):221-227
No abstract available.
Humans
4.Effect of VCAM-1 expression in human endothelial cells by proinflammatory cytokines.
Yun Hae CHANG ; Su Kil PARK ; Hee Bom MOON ; Jae Dam LEE ; Seong Who KIM ; On You HWANG ; Hae Nam HONG ; Young Joo CHO
Journal of Asthma, Allergy and Clinical Immunology 1999;19(2):163-172
BACKGROUND: The expression of adhesion molecules contribute to development of systemic diseases. Vascular cell adhesion molecule-l(VCAM-1) is an endothelial cell membrane glycoprotein that has been implicated in leukocyte/endothelial cell interactions in inflammation. OBJECTIVE: The aim of this study was to characterize the surface expression and regulation of VCAM-1 on two different endothelial cells. METHOD: We examined the effects of the expression of VCAM-1 in two different endothelial cells, isolated from human umbilical cords and human glomerulus. Expression of VCAM-1 was measured by enzyme-linked immunosorbent assay(ELISA) and flow cytometry. RESULTS: In human umbilical cord endothelial cells(HUVECs), both interleukin-l B(IL-lB) and tumor necrosis factor-a (TNF-a) increased VCAM-1 expression. VCAM-1 expression increased by TNF-a was higher than that increased by IL-lB. In human glomerular endothelial cells(HGECs), IL-lB and TNF-a markedly increased VCAM-1 expression. Conclusion. The regulation of VCAM-1 appears to be somewhat different in HGECs compared with HUVECs. These differences between the responsiveness of the two cells may possibly indicate inherent differences in endothelial cell derived from different vascular beds.
Cell Adhesion
;
Cell Communication
;
Cytokines*
;
Endothelial Cells*
;
Flow Cytometry
;
Humans*
;
Inflammation
;
Membrane Glycoproteins
;
Necrosis
;
Umbilical Cord
;
Vascular Cell Adhesion Molecule-1*
5.Two Cases of Postmyocardial Infarction Ventricular Septal Defect(VSD).
Hae Sim PARK ; Seung Jea TAHK ; Nam Sick CHUNG ; Won Heum SHIM ; Seung Yun CHO ; Woong Ku LEE
Korean Circulation Journal 1985;15(1):145-150
Perforation of the interventricular septum complicating acute myocardial infarction is uncommon. The condition was first described anatomically by Latham in 1845 and the first antemortem diagnosis was made by Brunn in 1923. In both cases, bedside catheterization utilizing a flow directed catheter detected a step up of O2 saturation at the ventricular level, compatible with ventricular septal defect, and two dimensional echocardiogram allowed direct visualization and localization of the postmyocardial infarction VSD. Finally cineventriculogram confirmed them.
Catheterization
;
Catheters
;
Diagnosis
;
Heart Septal Defects, Ventricular
;
Infarction*
;
Myocardial Infarction
6.Factors Associated with the Timing of First Sexual Intercourse Among College Students in Busan, Korea.
Young Hae KIM ; Nam Cheol PARK ; Hyun Jun PARK ; Eun Young YUN
Korean Journal of Andrology 2011;29(2):134-143
PURPOSE: The purpose of this study was to predict when college students in Korea have their first sexual intercourse and to explore the factors associated with the timing of first sexual intercourse. MATERIALS AND METHODS: Data are collected by using self-report survey toward total 797 of male and female college students who are currently attending the college in Busan, Korea. Students were divided by four groups according to their age of timing of first sexual intercourse, <16 years, between 16 to under 19, over 19 years and non-experienced. To examine the difference of four groups, Chi-square test and ANOVA (analysis of variance) were used. For estimation of the timing of first sexual intercourse we used Kaplan-Meier estimation and for differences of each group we used log-rank test. To identify the factors associated with the timing of first sexual intercourse we used Cox proportional hazards model. RESULTS: 41.4% of college students have experienced sexual intercourse. Of four groups the early sexual intercourse group (<16 years) had high rate of cigarette smoking, alcohol drinking, drug use, open sexual attitude, and a high number of sex partner. The factors associated with the timing of first sexual intercourse among college students was identified male, open parents attitude about sex, pornography experiences, cigarette smoking, alcohol drinking. CONCLUSIONS: This study predicts the timing of first sexual intercourse among college students in Korea. Early sexual debut is an important indicator for continued risk behavior regarding reproductive health.
Alcohol Drinking
;
Coitus
;
Erotica
;
Female
;
Humans
;
Kaplan-Meier Estimate
;
Korea
;
Male
;
Parents
;
Reproductive Health
;
Risk-Taking
;
Smoking
7.A Case of Apert's Syndrome with Encephalocele and Hypogenesis of Corpus Callosum.
Min Kyoung KWON ; Ja Yun YUN ; Mi Ran KIM ; Kon Hee LEE ; Hae Ran LEE ; Kwang Nam KIM
Journal of the Korean Pediatric Society 2001;44(7):832-836
Apert's syndrome(acrocephalosyndactyly) is a rare congenital anomaly that is characterized by the combination of premature fusion of multiple cranial sutures primarily involving the coronal suture and severe symmetrical syndactyly of fingers and toes. Some patients of this syndrome have various abnormalities of the central nervous system. We experienced a case of Apert's syndrome with craniofacial malformations, symmetric syndactyly, occipital encephalocele and hypogenesis of corpus callosum. She had a oxycephalic head with small sized anterior fontanelle, elongated forehead, hypertelorism, down-slanting palpebral fissures, flattened nose, cleft palate, low set ears and short neck. A 5x3 cm sized scalp-covered soft mass protruded in the occipital area through defect of the occipital bone. MRI showed hypogenesis of corpus callosum, occipital encephalocele, dilatation of the lateral and the third ventricles. She also had hands and feet with symmetric syndactyly involved skin and soft tissue but not bones.
Central Nervous System
;
Cleft Palate
;
Corpus Callosum*
;
Cranial Fontanelles
;
Cranial Sutures
;
Dilatation
;
Ear
;
Encephalocele*
;
Fingers
;
Foot
;
Forehead
;
Hand
;
Head
;
Humans
;
Hypertelorism
;
Magnetic Resonance Imaging
;
Neck
;
Nose
;
Occipital Bone
;
Skin
;
Sutures
;
Syndactyly
;
Third Ventricle
;
Toes
8.Autoregulation of Quartz-induced iNOS by iNOS-derived Hydrogen Peroxide in Rat2 Fibroblast.
Kyoung Ah KIM ; Hae Yun NAM ; Je Hyeok MUN ; Jin Sook JEONG ; Young LIM ; Chee Kyung CHUNG
Korean Journal of Occupational and Environmental Medicine 2001;13(4):385-392
OBJECTIVES: This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase(NOS) induced by alpha-quartz in Rat2 fibroblast. METHODS: alpha-quartz-induced nitric oxide(NO) and H2O2 formation and alpha- quartz-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor(L-N6- (1-iminoethyl)lysine hydrochloride, L-NIL) or antioxidant(catalase), we observed NO and H2O2 formation and iNOS protein expression in Rat2 fibroblast stimulated with alpha-quartz. RESULTS: alpha-quartz stimulated iNOS-induced NO and H2O2 formation in Rat2 fibroblast. L-NIL inhibited H2O2 formation and iNOS protein expression by alpha-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOSinduced H2O2, therefore H2O2 and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with alpha-quartz CONCLUSIONS: alpha-quartz-induced iNOS stimulated H2O2 formation in Rat2 fibroblast. INOS-induced H2O2 by alpha-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast
Catalase
;
Fibroblasts*
;
Homeostasis*
;
Hydrogen Peroxide*
;
Hydrogen*
;
Nitric Oxide
;
Quartz
9.The Role of TNFalpha Gene Promoter Polymorphism in the Development of Coal Workers' Pneumoconiosis.
Byoung Yong AHN ; Kyoung Ah KIM ; Hae Yun NAM ; Je Hyeok MUN ; Jin Sook JEOUNG ; Young LIM
Korean Journal of Occupational and Environmental Medicine 2002;14(2):117-123
OBJECTIVES: This study was performed in order to investigate the frequency of the TNF2 allele in patients with coal workers pneumoconiosis (CWP). METHODS: We compared the genotype distribution of TNFalpha gene promoter polymorphism between 80 CWP patients and 54 healthy controls. RESULTS: The results were as follows : 1. The rare allele TNF2 was significantly more frequent in CWP patients (20.6 %) than in controls (10.2 %). 2. The spontaneous or LPS-induced release of TNFalpha from the peripheral monocytes was slightly increased in the TNF2 group, but these values were not significantly different between groups. 3. In the CWP TNF2 group, the increase of LPS-induced TNFalpha release was significant in comparison with that of the controls. CONCLUSIONS: From the above results, we suggest that the TNF2 allele is strongly associated with susceptibility to CWP development.
Alleles
;
Anthracosis
;
Coal*
;
Genotype
;
Humans
;
Monocytes
;
Pneumoconiosis*
;
Tumor Necrosis Factor-alpha*
10.Activity of Telomerase in Coal Worker's Autopsied Lung.
Jin Suk CHUNG ; Hae Yun NAM ; Byoung Yong AHN ; Kyoung Ah KIM ; Young LIM
Korean Journal of Occupational and Environmental Medicine 2002;14(4):347-352
OBJECT: We measured the activity of telomerase in coal workers lung tissue and found a significant increase in telomerase activity compared to the control group. Pneumoconiosis has the characteristics of fibroblast proliferation and the accumulation of collagen,thus finally causing the pathologic changes,which lead to the irreversible and progressive fibrosis of the lungs. We hypothesized that this cellular proliferation causing irreversible fibrosis may induce some elongation of the life cycle in the chromosomes and lead to further cellular immortalization. METHOD: 8 postmortem(within 24 hours)pneumo-coniotic cases were examined and their telomerase activity was compared with that of the autopsied lungs of lung cancer patients and of accident victims without any respiratory diseases. Using the extracted ribo-nucleoprotein from pneumoconiotic nodules, telomeric repeat amplification assay (TRAP)was done. RESULT: The pneumoconiotic lungs showed strong telomerase activity, similar to that of the lung cancer patients, while the control group showed no such activity. CONCLUSION: Based on the results of this study, we found that coal dust-induced cellular proliferation affects telomerase-activity clinically.
Cell Proliferation
;
Coal*
;
Fibroblasts
;
Fibrosis
;
Humans
;
Life Cycle Stages
;
Lung Neoplasms
;
Lung*
;
Pneumoconiosis
;
Telomerase*