Human rotavirus has now been established as the leading cause of gastroenteritis in young children worldwide. At least fourteen serotypes of group A rotavirus have been identified on the basis of antibody responses to major neutralizing glycoprotein, VP7 (G type for glycoprotein), present in the outer capsid of the virus. Serotype 1, 2, 3 and 4 are the most highly prevalent in human. In Korea, rotavirus is also the principal cause of severe nonbacterial diarrhea requiring hospitalization in infants and young children, which is commonly detected by EIA method. The epidemiology of rotavirus infection has been monitored by only serologic methods without electropherotyping in Korea. This study shows seasonal and age related variations .of rotavirus infection in Korea according to the genotype using the reverse transcription polymerase chain reaction (RT-PCR). Fecal specimens were obtained from 39 children hospitalized with acute watery diarrhea and gastroenteritis in Ewha Womans University MokDong Hospital in Seoul from Jan. to Dec. of 1996. All four (1, 2, 3, 4) major G serotypes were identified by amplification of segment of the gene for VP7 using RT-PCR. Rotavirus Gl 749 bp, G2 653 bp, G3 374 bp and G4 583bp were shown on 2.9 or 3.3% NuSieve agar gel. Results were as follows: 1) Rotavirus was detected at 53.8% (21/39) by EIA and 89.7% (35/39) by RT-PCR. 2) Serotype Gl, G2, G3, G4 when detected by RT-PCR accounted for 80.0% (28/35), 14.3% (5/35), 2.9% (1/35) and 2.9% (1/35), respectively. 3) Thirty five strains of rotavirus were detected at the frequency of 17.1% (6/35) in Oct., 20.0% (7/35) in Nov. and 20.0% (7/35) in Dec. 4) As for the age range, children affected by rotavirus were mostly under 1 years.
Agar ; Antibody Formation ; Capsid ; Child ; Child, Hospitalized* ; Diarrhea* ; Epidemiology ; Female ; Gastroenteritis ; Genotype* ; Glycoproteins ; Hospitalization ; Humans* ; Infant ; Korea ; Polymerase Chain Reaction* ; Reverse Transcription* ; Rotavirus Infections ; Rotavirus* ; Seasons ; Seoul

Astrovirus is frequently associated with diarrhea in children. It can not be readily isolated by cell culture, and an electronmicroscope is usually used for detection of this agent. Recently in 1995 a combined method of reverse transcription-polymerase chain reaction (RT-PCR) was designed for easier detection of astrovirus, which is based on the conserved sequence in 3'-end of genomes of the 7 known serotypes of human astrovirus. As of yet there has not been any report of astrovirus data in Korea using the RT-PCR methods. The purpose of this study was to detect astrovirus incidence, severity of symptoms, seasonal variation and coinfection rate with rotavirus in Korean children inpatients with diarrhea. Fecal specimens from 61 young children hospitalized with gasteroenteritis Korea from Jan. 1996 through Mar. 1997. They were examined for astroviurs infection by RT-PCR method. Results are as follows: 1. Astrovirus was detected at 9.8% (6/61) from fecal specimens of children with severe diarrhea by EIA using monoclonal antibody coated plates. 2. Astorvirus was detected at 29.5% (18/61) from fecal specimens of children with severe diarrhea by RT-PCR. 3. The age of the 18 children affected by astrovirus ranged from 2 monthes to 7 years with mean of 3.0 years. 4. Mean hospital stay of the 1S children was 6.1 days. 5. Five (27.8%) astrovirus RT-PCR positive strains were confirmed in November and in December, respectively out of 18 specimens in total. 6. Astrovirus coinfection with rotavirus type G1 was confirmed in 15/16 specimens (93.8%), and with type G2 was in 1/16 specimens (6.3%).
Cell Culture Techniques ; Child* ; Coinfection ; Conserved Sequence ; Diarrhea ; Feces* ; Genome ; Humans ; Incidence ; Inpatients ; Korea ; Length of Stay ; Mamastrovirus ; Polymerase Chain Reaction* ; Reverse Transcription* ; Rotavirus ; Seasons

This is case report of true class III malocclusion with macroglossia is corrected by glossectomy in 13 years female patient. After orthodontic treatment, the patient is bound to glossectomy because the corrected condition is relapsed to the previous condition due to relatively enlarged tongue compared with the original dental arch. By the interpretation of the cephalogram and model analysis, it is approved that the growth pattern and direction are normal range and mandible is located anterioly to the cranium. The results are follows: 1. We could treat the true Cl III malocclusion. 2. We could prevent the relapse of the treated condition by the surgical intervention, such as partial glossectomy. 3. Sensory, speech, swallowing and so other functions after the operation have been with in normal limit without any serious complications or seguellae.
Deglutition ; Dental Arch ; Female ; Glossectomy ; Humans ; Macroglossia* ; Malocclusion* ; Mandible ; Recurrence ; Reference Values ; Skull ; Tongue

No Abstract Available.
Adult* ; Cerebrospinal Fluid* ; Encephalitis* ; Humans ; Meningitis, Aseptic* ; Polymerase Chain Reaction*

We tried to treat 16 patients with superficial transitional cell carcinoma of the bladder with prophylactic and therapeutic intravesical chemotherapy. The 2 drugs used were adriamycin and thio-tepa and checked for efficacy by repeated cystoscopy with at least 3 months` interval. The clinical data and results of both groups are presented and the importance of intravesical treatment with minimal toxicity is discussed with a brief review of the literatures.
Carcinoma, Transitional Cell ; Cystoscopy ; Doxorubicin ; Drug Therapy* ; Humans ; Thiotepa ; Urinary Bladder Neoplasms* ; Urinary Bladder*

The prognostic significance of vascular (blood and lymphatic vessels) invasion was evaluated in a retrospective review of 27 patients with upper urinary tract transitional cell carcinoma from January 1985 to December 1993, who underwent a nephroureterectomy with bladder cuff resection. Vascular invasion was found in 10 patients (37%). The incidence of vascular invasion was well correlated with tumor grade and stage. The incidence of postoperative metastases was significantly higher in the patients with (70%) than without (17.6%) vascular invasion (p<0.05). The survival rate of the patients with vascular invasion was significantly lower than in those without vascular invasion (p<0.01). In multivariate Cox`s regressional analysis the prognostic value of vascular invasion was independent of tumor grade and stage. These results indicate that vascular invasion should predict a more unfavorable outcome in patients with upper urinary tract transitional cell carcinoma as an independent morphological indicator.
Carcinoma, Transitional Cell* ; Humans ; Incidence ; Lymphatic Vessels* ; Neoplasm Metastasis ; Prognosis ; Retrospective Studies ; Survival Rate ; Urinary Bladder ; Urinary Tract*

The mechanism of erectile dysfunction associated with psychotherapeutic medication is not well defined. To determine whether psychotherapeutic drugs have a direct effect on penile cavernosal smooth muscle, the activity of 4 drugs from 4 major classes of psychotherapeutic agents, chlorpromazine, thioridazine, haloperidol and amitriptyline, was examined in vitro on isolated rabbit corpus cavernosum. Strips of corpus cavernosum were mounted in organ baths for isometric tension studies. The effect of the drugs on resting tension, their relaxant effect on norepinephrine (NE) precontracted tissue, and their effect on electrical field stimulated (EFS) relaxation of NE precontracted tissue were determined. Incubation with any of the drugs did not affect resting tension compared to a control. All drugs produced a dose-dependent relaxation in NE precontracted tissue which were significantly greater than the water treated control (p<0.0001). None of the drugs inhibited EFS relaxation. These result show that these classes of drugs do not affect the basal tone of the corpus cavernosum. All have intrinsic relaxant properties and none interfere with neurally stimulated relaxation. This study suggests that drugs from these 4 major classes of psychotherapeutic agents do not adversely affect penile cavernosal smooth muscle relaxation at the level of the penis and its associated neural elements. It may be inferred that reports of impotence in patients treated with these classes of drugs more likely reflect a central or primary psychopathologic process rather than a local corpus cavernosum effect.
Amitriptyline ; Baths ; Chlorpromazine ; Erectile Dysfunction ; Haloperidol ; Humans ; Male ; Muscle, Smooth ; Norepinephrine ; Penis ; Relaxation ; Thioridazine ; Water

PURPOSE: This study was to test the reliability and validity of a Drinking Refusal Self-Efficacy Questionnaire-Revised (DRSEQ-R) in Korean college students. METHODS: The DRSEQ-R was designed to assess an individual's belief in their ability to refuse drinking alcohol by Oei et al. (2005) and consisted of three factors (social pressure, opportunity, and emotional relief). A methodological study design with an exploratory factor analysis for validity and correlation coefficients for reliability was used. DRSEQ-R was translated into Korean and a translation equivalency was obtained. DRSEQ-R was tested with a sample of 201 college students in Korea. The subjects consisted of 106 males and 95 females. Principal component factor analysis was used for construct validity and Cronbach's alpha was used to evaluate for internal consistency of the instrument. RESULTS: The factor analysis showed three factors explaining 66.3% of total variance and the corresponding factors were emotional relief (48.01%), drinking contextual opportunity (11.18%), and social pressure (7.14%). The internal consistency was acceptable (Cronbach's alpha=.937). All three factors of DRSEQ-R negatively correlated with alcohol dependency and a heavy drinker showed a lower DRSEQ-R than a moderate drinker. CONCLUSION: Based on the findings, DRSEQ-R is a reliable and valid instrument to measure for drinking refusal self-efficacy in Korean college students.
Alcohol Drinking/*prevention & control ; Factor Analysis, Statistical ; Female ; Humans ; Interpersonal Relations ; Male ; *Questionnaires ; Reproducibility of Results ; *Self Efficacy ; Social Behavior ; Students/*psychology ; Translating ; Universities ; Young Adult

No abstract available.
Meconium* ; Peritonitis*

No abstract available.
Sarcoma 180* ; Sarcoma*