1.Proteomic Analysis of DJ-1 Expression in the Muscles of Spastic Cerebral Palsy Patients.
Yoon Hae KWAK ; Soo Bong HAHN ; Sun Young KONG ; Seoung Woo HONG ; Hyun Woo KIM
Journal of Korean Orthopaedic Research Society 2008;11(2):45-55
PURPOSE: Using proteomic analysis, this study was performed to see the characteristics of proteins expression in the muscles of spastic cerebral palsy patients. MATERIALS AND METHODS: We studied twelve specimens from six patients with spastic cerebral palsy, three patients with myelomeningocele, and three normal people who underwent orthopaedic surgeries due to trauma. We studied the extracted proteins showing differences in the two-dimensional electrophoresis, and the prominent thirteen proteins were re-evaluated by proteomics and the reverse transcriptional polymerase chain reaction, which was to clarify the relationship between gene and protein expression. RESULTS: Among fifteen proteins, six proteins were found to be higher in normal people, and nine were found to be higher in the groups of patients by spot histogram. The results of proteomic analysis with MALDI-TOF for fifteen proteins showed that the expression of DJ-1 was related to cerebral palsy. CONCLUSION: This study shows that strong expression of DJ-1 is related to spasticity and cerebral palsy. We showed for the first time the possibility of any relationship between spastic condition and DJ-1 expression.
Cerebral Palsy
;
Electrophoresis
;
Humans
;
Meningomyelocele
;
Muscle Spasticity
;
Muscles
;
Polymerase Chain Reaction
;
Proteins
;
Proteomics
2.A Case of Lymphangiomas of the Esophagus and Sigmoid Colon.
Joo Ho KIM ; Bong Jin JUNG ; Yong Min SHIN ; Dong Soo PARK ; Kyu Sun AHN ; Oh Young KIM ; Kwang Ung RI ; Hyun Taek OH ; Hae Seoung YOON
Korean Journal of Gastrointestinal Endoscopy 1997;17(4):519-522
Lymphangiomas are benign tumors of lymphatic vessels, which can occur anywhere the lymphatics exist. The lymphangioma of the esophagus is exceedingly rare. Seven cases had reported in the world and there is no case report about the lymphangioma of the esophagus in our country. So we present one case of lymphangiomas that coexist in esophagus and sigmoid colon. This kind of case don't have been reported yet.
Colon, Sigmoid*
;
Esophagus*
;
Lymphangioma*
;
Lymphatic Vessels
3.A Case of Esophageal Ucerations in Behcet's Disease its Endoscopic Finding.
Bong Jin JUNG ; Yong Min SHIN ; Dong Soo PARK ; Kyu Sun AHN ; Oh Young KIM ; Ju Ho KIM ; Kwang Ung RI ; Hyun Taek OH ; Hae Seoung YOON ; Hyun I SHON
Korean Journal of Gastrointestinal Endoscopy 1997;17(4):513-516
Behcet's disease is a chronie inflammatory symptom complex consisting of recurrent oral ulcer, genital ulcer, ocular lesion or skin lesion. Intestinal involvement in Behcet's disease most commonly affects the ileocecal region in the patient with gestrointestinal symptoms, But esophageal involvement in Behcet's disease is very uncommon, most commonly affects the midesophagus with localized ulcerative lesion. A 38-Year-old woman with refractory and recurrent oral and genital ulcer was admitted because of aggravation of pharyngodynia and dysphagia. Gastrofiberscopic examination showed multiple, small sized ulcers on oral cavity and mid-to-distal esophagus. She was treated with steroid.
Adult
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Deglutition Disorders
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Esophagus
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Female
;
Humans
;
Mouth
;
Oral Ulcer
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Skin
;
Ulcer
4.A Case of Nonfamilial Benign Recurrent Intrahepatic Cholestasis.
Oh Young KIM ; Bum Yong SUNG ; Gyo Don KOWG ; Hae Seoung YOON ; Yong Min SHIN ; Hyun Taek OH ; Mi Kyoung KIM ; Hyeong Kweon KIM ; Ju Ho KIM ; Kwang Ung RI ; Hyun I SHON
The Korean Journal of Hepatology 1998;4(2):188-193
Benign recurrent intrahepatic cholestasis (BRIC) is a rare desease, which usually manifests between the age of 10 and 20. Its main clinical feature is multiple recurrent episodes of cholestasis without extrahepatic bile duct obstruction. We report here a case of nonfamilial benign recurrent intrahepatic cholestasis. The patient has experienced recurrent jaundice with pruritus since childhood. Main bile duct obstrution was excluded by abdominal CT and endoscopic retrograde cholangiopancreatography. Other causes of cholestasis were not found. Hepatic histology revealed bile plug which were mainly concentrated in the centrilobular region, and increased number of mononuclear cells in the portal triad, but hepatic parenchyma showed no inflammation and necrosis. In the last anicteric period, she was healthy and the liver function test and biopsy specimen were normal.
Bile
;
Bile Ducts
;
Bile Ducts, Extrahepatic
;
Biopsy
;
Cholangiopancreatography, Endoscopic Retrograde
;
Cholestasis
;
Cholestasis, Intrahepatic*
;
Humans
;
Inflammation
;
Jaundice
;
Liver Function Tests
;
Necrosis
;
Pruritus
;
Tomography, X-Ray Computed
5.A Case of Nonfamilial Benign Recurrent Intrahepatic Cholestasis.
Oh Young KIM ; Bum Yong SUNG ; Gyo Don KOWG ; Hae Seoung YOON ; Yong Min SHIN ; Hyun Taek OH ; Mi Kyoung KIM ; Hyeong Kweon KIM ; Ju Ho KIM ; Kwang Ung RI ; Hyun I SHON
The Korean Journal of Hepatology 1998;4(2):188-193
Benign recurrent intrahepatic cholestasis (BRIC) is a rare desease, which usually manifests between the age of 10 and 20. Its main clinical feature is multiple recurrent episodes of cholestasis without extrahepatic bile duct obstruction. We report here a case of nonfamilial benign recurrent intrahepatic cholestasis. The patient has experienced recurrent jaundice with pruritus since childhood. Main bile duct obstrution was excluded by abdominal CT and endoscopic retrograde cholangiopancreatography. Other causes of cholestasis were not found. Hepatic histology revealed bile plug which were mainly concentrated in the centrilobular region, and increased number of mononuclear cells in the portal triad, but hepatic parenchyma showed no inflammation and necrosis. In the last anicteric period, she was healthy and the liver function test and biopsy specimen were normal.
Bile
;
Bile Ducts
;
Bile Ducts, Extrahepatic
;
Biopsy
;
Cholangiopancreatography, Endoscopic Retrograde
;
Cholestasis
;
Cholestasis, Intrahepatic*
;
Humans
;
Inflammation
;
Jaundice
;
Liver Function Tests
;
Necrosis
;
Pruritus
;
Tomography, X-Ray Computed
6.A Clinical Analysis of Thyroglossal Duct Cyst and Sinus.
Yong Suk CHO ; Sung Gil PARK ; Seong Eun CHON ; Dae Kun YOON ; Bong Wha CHUNG ; Jun HUR ; Ma Hae CHO ; Ji Woong CHO ; Yoon Kyu PARK ; Seoung Wan CHAE ; Seong Jin CHO ; Hye Kyung AHN
Journal of the Korean Surgical Society 2003;64(3):189-193
PURPOSE: Anomalies in the thyroglossal duct are the most common midline, or paramedian cervical lesions of congenital origin. Presenting as a thyroglossal duct cyst (TGDC) or a thyroglossal duct sinus (TGDS), they are found in all age groups. This clinical study was intended to elucidate the clinical characteristics, and the outcomes, of surgical treatment of these anomalies. METHODS: Between January 1992 and May 2002, 91 patients underwent surgery for a TGDC or a TGDS at 4 hospitals affiliated to Hallym University. The demographics of the patients, the clinical characteristics of the lesions, and the outcomes of the treatments were retrospectively evaluated. These characteristics were evaluated according to age groups; younger than 15 (38 patients) and older than 15 (53 patients) years. RESULTS: The male to female ratio was 1.67: 1 (It is better to give the actual figures.), with the first decade showing the highest incidence in the age distribution (33%). Painless masses (83.6%) were the most common presenting symptom, with most symptoms having been manifest for less than 1 year (67%). TGDCs were 90% and TGDSs were 10%. There were 62 cases of infrahyoid and 18 of suprahyoid lesions, with 80 cases on the midline and 11 on the paramedian within 2 cm from the midline. Of the paramedian lesions, 2 cases were in the younger group and 9 were in the older group. This tendency of laterality in the location by age group showed no statistical significance (p=0.172). All the patients underwent a Sistrunk operation, and one experienced a recurrence. CONCLUSION: This study justified the Sistrunk operation as the treatment of choice for anomalies of the thyroglossal duct. The lateral lesions occurred in the old age groups more frequently. A careful approach is needed with paramedian cervial lesions in adults to avoid the loss of the proper treatment of possible hidden congenital lesions.
Adult
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Age Distribution
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Demography
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Female
;
Humans
;
Incidence
;
Male
;
Recurrence
;
Retrospective Studies
;
Thyroglossal Cyst*
7.Occurrence and characterization of oseltamivir-resistant influenza virus in children between 2007-2008 and 2008-2009 seasons.
Seoung Geun KIM ; Yoon Ha HWANG ; Yung Hae SHIN ; Sung Won KIM ; Woo Sik JUNG ; Sung Mi KIM ; Jae Min OH ; Na Young LEE ; Mun Ju KIM ; Kyung Soon CHO ; Yeon Gyeong PARK ; Sang Kee MIN ; Chang Kyu LEE ; Jun Sub KIM ; Chun KANG ; Joo Yeon LEE ; Man Kyu HUH ; Chang Hoon KIM
Korean Journal of Pediatrics 2013;56(4):165-175
PURPOSE: There was a global increase in the prevalence of oseltamivir-resistant influenza viruses during the 2007-2008 influenza season. This study was conducted to investigate the occurrence and characteristics of oseltamivir-resistant influenza viruses during the 2007-2008 and 2008-2009 influenza seasons among patients who were treated with oseltamivir (group A) and those that did not receive oseltamivir (group B). METHODS: A prospective study was conducted on 321 pediatric patients who were hospitalized because of influenza during the 2007-2008 and 2008-2009 influenza seasons. Drug resistance tests were conducted on influenza viruses isolated from 91 patients. RESULTS: There was no significant difference between the clinical characteristics of groups A and B during both seasons. Influenza A/H1N1, isolated from both groups A and B during the 2007-2008 and 2008-2009 periods, was not resistant to zanamivir. However, phenotypic analysis of the virus revealed a high oseltamivir IC50 range and that H275Y substitution of the neuraminidase (NA) gene and partial variation of the hemagglutinin (HA) gene did not affect its antigenicity to the HA vaccine even though group A had a shorter hospitalization duration and fewer lower respiratory tract complications than group B. In addition, there was no significant difference in the clinical manifestations between oseltamivir-susceptible and oseltamivir-resistant strains of influenza A/H1N1. CONCLUSION: Establishment of guidelines to efficiently treat influenza with oseltamivir, a commonly used drug for treating influenza in Korean pediatric patients, and a treatment strategy with a new therapeutic agent is required.
Child
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Drug Resistance
;
Hemagglutinins
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Hospitalization
;
Humans
;
Influenza, Human
;
Inhibitory Concentration 50
;
Neuraminidase
;
Orthomyxoviridae
;
Oseltamivir
;
Prevalence
;
Prospective Studies
;
Respiratory System
;
Seasons
;
Viruses
;
Zanamivir
8.Spontaneous Rupture of the Esophagus (Boerhaave's Syndrome): A review of 10 cases.
Dong Joon SHIN ; Gi Young SUNG ; Wook KIM ; Hae Myung JEON ; Yoon Bok LEE ; Seoung Chul PARK ; Min Kwang HONG ; Moo Hyung SONG ; Do Sang LEE ; Il Young PARK ; Jong Man WON ; Nam Ik HAN
Journal of the Korean Surgical Society 1999;57(2):210-216
BACKGROUND: Boerhaave's syndrome is a spontaneous rupture of the esophagus. The classic symptom triad, vomiting, chest pain, and subcutaneous emphysema, rarely develop together. It is diagnosed by using plain chest film, an esophagogram with a water soluble contrast media, and computerized tomography, but it is somewhat difficult to make an early diagnosis. It is generally treated with a surgical procedure, but the most frequent complication is suture line leakage, which leads to a pyothorax, pneumonia, mediastinitis, and eventually an irreversible septic condition. METHODS: We retrospectively reviewed 10 cases of patients with spontaneous esophageal rupture treated by various surgical methods during the recent 6 years at the Department of Surgery and Internal Medicine, the Catholic University of Korea. RESULTS: The results of the clinical reviews are as follows: There were 9 males and 1 female, and the mean age was 53 years old. The main symptom was chest pain (70%), and that developed after severe vomiting in 6 cases (60%). Abnormal findings were revealed on the chest PA films of 6 cases. Esophagogram were used in 8 cases, and a combined chest CT in 5 cases. Seven (70%) received an operation in less than 24 hours after the attack and 3 cases after 72 hours. The lower one-third of the esophagus was perforated in 9 cases (90%), and the left side of the esophagus was perforated in 8 cases (80%). The methods of operation were primary repair (6 cases), an esophagectomy and esophagogastrostomy (3 cases), and a cardial ligation, proximal esophagostomy, gastrostomy, and feeding jejunostomy (1 case). Anastomosis leakage and empyema developed in each of 4 cases. The overall mortality rate was 20%; only two patients died due to a delayed hospital visit (surgery 72 hours after first attack of symptoms). CONCLUSIONS: We recommand that the most important factor affecting the prognosis for spontaneous rupture of the esophagus is early diagnosis and immediate surgical intervention.
Chest Pain
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Contrast Media
;
Early Diagnosis
;
Empyema
;
Empyema, Pleural
;
Esophagectomy
;
Esophagostomy
;
Esophagus*
;
Female
;
Gastrostomy
;
Humans
;
Internal Medicine
;
Jejunostomy
;
Korea
;
Ligation
;
Male
;
Mediastinitis
;
Middle Aged
;
Mortality
;
Pneumonia
;
Prognosis
;
Retrospective Studies
;
Rupture
;
Rupture, Spontaneous*
;
Subcutaneous Emphysema
;
Sutures
;
Thorax
;
Tomography, X-Ray Computed
;
Vomiting