1.Study on cord blood hemoglobin and etiology of neonatal anemia.
Chul LEE ; Hae Jung CHO ; Myung Ho LEE ; Sook Ja PARK ; Young Hae LEE
Journal of the Korean Pediatric Society 1982;25(9):906-913
No abstract available.
Anemia, Neonatal*
;
Fetal Blood*
;
Infant, Newborn
2.Anti-Cancer Efficacy of Anti-CEA IgG3 in a Syngeneic Carcinoembryonix Antigen Tumor Model.
Hyun Mi CHO ; Hae Jung KIM ; Mi Young CHO ; Seung Uon SHIN
Korean Journal of Immunology 1999;21(2):129-135
Development of antibody-based cancer therapies will be greatly facilitated if antibodies are better standardized in two fundamental issues that are specificity analysis of antibody reactivity and the detailed biodistribution and pharmacokinetic profile of antibodies. In the current endeavor we attempted to use an antibody binding specificity to target the tumor in a syngeneic carcinoembryonic antigen (CEA) tumor model. CEA, a 180 kDa glycoprotein, expressed at high levels on the surface of nearly all tumors of the gastrointestinal tract was used a potential target for antibody immunotherapy of gastrointestinal carcinomas. Using the CEA model antibody-based cancer therapy directed against CEA has been evaluated in a syngeneic animal model of disseminated disease. We constructed mouse/human chimeric anti-CEA IgG3, which has been evaluated for the specificity for CEA and the detailed biodistribution and pharmacokinetic profiles. Anti-CEA IgG3 heavy chain was expressed with the expected 180kDa molecular weight, assembled as H2L2 forms with a co-expressed mouse/human chimeric anti-CEA light chain, and were secreted. On FACS the purified anti-CEA IgG3 specifically recognized the mouse colon adenocarcinoma cell line MC-38 transduced with CEA (MCA32a), but not MC 38 without expressing CEA. After subcutaneous injection in C57BL/6 mice the half- lives of anti-CEA IgG3 and an irrelevant anti-dansyl IgG3 showed the bi-phasic kinetic patterns, and their pharmacokinetics of the distribution and the elimination were similar in mice. However, the biodistribution patterns of anti-CEA IgG3 were very different from those of anti-dansyl IgG3. Anti-dansyl IgG3 was mainly distributed into kidney until 72 hours, but anti-CEA IgG3 was slowly rernoved from blood and distributed into liver, kidney, spleen, and tumor. It is note worthy that anti- CEA IgG3 increased in targeting MCA32a tumor expressing human CEA by time, but the targeting to MC38 tumor was negligible. Thus, the increased targeting of anti- CEA IgG3 made MCA32a tumor grow slowly
Adenocarcinoma
;
Animals
;
Antibodies
;
Carcinoembryonic Antigen
;
Cell Line
;
Colon
;
Gastrointestinal Tract
;
Glycoproteins
;
Humans
;
Immunoglobulin G*
;
Immunotherapy
;
Injections, Subcutaneous
;
Kidney
;
Liver
;
Mice
;
Models, Animal
;
Molecular Weight
;
Pharmacokinetics
;
Sensitivity and Specificity
;
Spleen
3.Affinity Improvement of Antibody-Avidin Fusion Proteins for Biotin.
Mi Young CHO ; Hae Jung KIM ; Hyun Mi CHO ; Seung Uon SHIN
Korean Journal of Immunology 1998;20(4):381-388
To generate drug delivery vector to locales in the body, genetic engineering and expression techniques have been used to produce antibody avidin fusion proteins. Chicken avidin has been fused to mouse-human chimeric IgG3 immediately after the hinge with a flexible linker (H-Flex-Av) and at the end of CH2 (CH2-Av). Fusion heavy chains were expressed with the expected molecular weight, assembled as H2L2 forms with a co-expressed light chain, and were secreted. The expression level of H- Flex-Av was 1~10 ug/ml/10(8)/24 hrs, but that of C2-Av was a very little (0.08~0.9 ug/ ml/10(8)/24 hrs). The resulting H-Flex-Av and CH2-Av fusion proteins continued to bind antigen dansyl and also bound biotinylated bovine serum albumin; both H-Flex-Av and CH2-Av had shown to retain 3-4 times higher relative affinity than that of CH3-Av in ELISA. Importantly the fact that both avidin fusion proteins had a higher relative affinity suggests that these avidin fusion proteins can be effectively used to deliver biotinylated ligands such as drugs and peptides to a certain locale, such as the brain.
Avidin
;
Biotin*
;
Brain
;
Chickens
;
Enzyme-Linked Immunosorbent Assay
;
Genetic Engineering
;
Immunoglobulin G
;
Ligands
;
Molecular Weight
;
Peptides
;
Serum Albumin, Bovine
4.A case of Dubin-Johnson Syndrome.
Ae Jung KWAK ; Mi jung KIM ; Min Jung CHO ; Kwang Hae CHOI
Yeungnam University Journal of Medicine 2002;19(1):68-72
Dubin-Johnson Syndrome is a form of benign, familial idiopathic jaundice presenting with chronic intermittentconjugated hyperbilirubinnmia and a melamin-like pigment has been found in the parenchymal liver cells. This disorder is rarely diagnosed in the neonatal period. We report a case of Dubin-Johnson syndrome presenting with neonatal cholestasis.
Cholestasis
;
Jaundice
;
Jaundice, Chronic Idiopathic*
;
Liver
5.A case of Dubin-Johnson Syndrome.
Ae Jung KWAK ; Mi jung KIM ; Min Jung CHO ; Kwang Hae CHOI
Yeungnam University Journal of Medicine 2002;19(1):68-72
Dubin-Johnson Syndrome is a form of benign, familial idiopathic jaundice presenting with chronic intermittentconjugated hyperbilirubinnmia and a melamin-like pigment has been found in the parenchymal liver cells. This disorder is rarely diagnosed in the neonatal period. We report a case of Dubin-Johnson syndrome presenting with neonatal cholestasis.
Cholestasis
;
Jaundice
;
Jaundice, Chronic Idiopathic*
;
Liver
6.A case of prenatal diagnosed ectopia cordis by ultrasonography.
Yong Suk JUNG ; Kae Hyun NAM ; Kwon Hae LEE ; Tai Ho CHO
Korean Journal of Obstetrics and Gynecology 1992;35(8):1233-1237
No abstract available.
Ectopia Cordis*
;
Ultrasonography*
7.The usefulness of tumor markers SCCA and CEA in squamous cell carcinoma of the uterine cervix.
Jung Jae LEE ; Kae Hyun NAM ; Soon Gon LEE ; Kwon Hae LEE ; Tai Ho CHO
Korean Journal of Obstetrics and Gynecology 1993;36(7):1313-1321
No abstract available.
Carcinoma, Squamous Cell*
;
Cervix Uteri*
;
Female
;
Biomarkers, Tumor*
8.Relationship of Maternal and Cord Serum Ferritin.
Hae Sung CHO ; Jin Hyun PARK ; Hee Jung KWON ; In Sil LEE
Journal of the Korean Pediatric Society 1988;31(11):1453-1459
No abstract available.
Ferritins*
9.Effect of Asthma Management Educational Program on The Disease Related Knowledge, Stress, and Self-efficacy of Asthmatics Allergic to House Dust Mite.
Yang Sook YOO ; Ok Hee CHO ; Hae Sun JUNG
Journal of Korean Academy of Adult Nursing 2004;16(4):617-625
PURPOSE: The purpose of this study was to investigate the effect of the asthma management educational program given to the allergic asthmatics receiving immunotherapy due to house dust mite on the disease related to knowledge, stress, and self-efficacy. METHOD: The subjects of this study were the patients received immunotherapy to house dust mite at a week interval after being diagnosed for house dust mite allergic asthma at the respiratory center of a hospital affiliated to the university. They were divided into the experimental group of 29 patients who received asthma management education, the control group of 32 patients, and 61 patients in total. Experimental treatment, as an asthma management educational program, was the group education of one time and the reinforcement education of three times with environmental therapy and immunotherapy to house dust mite. RESULTS: The results revealed that the improvement in disease related knowledge, the improvement in self-efficacy and the decrease in stress, were significantly higher in the experimental group than the control group. CONCLUSIONS: The asthma management educational program had an effect on improving the disease related knowledge and self-efficacy, and decreasing the stress of the patients asthmatics allergic to house dust mite.
Asthma*
;
Dust*
;
Education
;
Humans
;
Immunotherapy
;
Pyroglyphidae*
;
Respiratory Center
10.A Case of Wilson-Mikity Syndrome.
Jang Kwun YANG ; Byoung Gug JUNG ; Hae Sung CHO ; In Sil LEE
Journal of the Korean Pediatric Society 1987;30(9):1055-1059
No abstract available.