This study is conducted to determine the effects of dietary source of omega3 fatty acids on preneoplastic foci and the glutathione dependent enzymes in rat hepatocarcinogenesis initiated by diethylnitrosamine (DEN). Male Sprague-Dawley rats were fed one of three diets containing 10% (w/w) fats fixed p/s = -1.0 and omega6/omega3 ratio = -0.4 or 4.0 ; fish oil-com oil blended (FC), com oil-beef tallow-fish oil blended (CF), com oil-beef tallow-perilla oil blended (CP), from gestation period. At 10 weeks, animals of experimental groups were injected intraperitoneally with DEN (200 mg/kg body weight) and two-thirds partial hepatectomy was carried out 3 weeks later and were sacrificed 8 weeks after DEN initiation. The area and number of glutathione S-transferase placenta (GST-P) positive foci were significantly decreased in rats fed diets containing fish oil (FC and CF) than those fed perilla oil diet (CP). Fish oil feeding significantly increased the activities of glutathione dependent enzymes. Rats fed diets containing fish oil (FC and CF) significantly increased the glutathione (GSH) content and the activities of glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST). Glutathione dependent enzymes had significantly negative correlation with GST-P positive foci. Glucose 6-phosphatase (G6Pase) was increased in rats feeding fish oil. Thiobarbituric acid reactive substances were not different among groups. Therefore, the preventive effect against hepatocarcinogenesis might be explained by induction of the glutathione dependent enzymes and G6Pase.
Animals ; Diet ; Diethylnitrosamine ; Fats ; Fatty Acids* ; Glucose-6-Phosphatase ; Glutathione Peroxidase ; Glutathione Reductase ; Glutathione Transferase ; Glutathione* ; Hepatectomy ; Humans ; Male ; Perilla ; Placenta ; Pregnancy ; Rats* ; Rats, Sprague-Dawley ; Thiobarbituric Acid Reactive Substances

Purpose: This study examined the nutritional status and Korean Healthy Eating Index (KHEI) scores pre- and post-coronavirus disease 2019 (COVID-19) outbreak in elderly men and women living in rural areas. Methods: The participants were 1,747 rural elderly people aged 65 or older who participated in the nutritional survey of the 2018–2021 Korea National Health and Nutrition Survey. The nutrient intake was estimated from a 24-hour recall. Results: Following the COVID pandemic, the intake of fat, saturated fatty acids, and sodium increased in elderly men and women, whereas the intake of thiamine decreased. In elderly men, the intake of protein, niacin, and vitamin D decreased, but the sugar intake increased.In elderly women, the cholesterol intake increased. The KHEI score was significantly lower in rural elderly men and women after the pandemic than before (male: 62.60 ± 0.56 for the post-COVID-19 and 64.76 ± 0.75 for the pre-COVID-19, p < 0.05; female: 64.07 ± 0.80 for the post-COVID-19 and 64.47 ± 0.62 for the post-COVID-19, p < 0.001). Regarding the KHEI component, in men, the intake of meat, fish, and legumes, all sources of protein, decreased significantly after the COVID-19 pandemic. In women, the total food intake and the intake of breakfast decreased significantly after the pandemic. In the moderation components, men were unable to limit their total sugar and sodium intake, while women were unable to control their saturated fatty acids and sodium intake. Furthermore, considering the total KHEI score, both elderly men and women had decreased values in the moderation and adequacy components following COVID-19. Conclusion These results provide foundational data for elderly nutrition policies or educational programs targeting the elderly population in rural areas in similar pandemic situations in the future.

Purpose: This study examined the nutritional status and Korean Healthy Eating Index (KHEI) scores pre- and post-coronavirus disease 2019 (COVID-19) outbreak in elderly men and women living in rural areas. Methods: The participants were 1,747 rural elderly people aged 65 or older who participated in the nutritional survey of the 2018–2021 Korea National Health and Nutrition Survey. The nutrient intake was estimated from a 24-hour recall. Results: Following the COVID pandemic, the intake of fat, saturated fatty acids, and sodium increased in elderly men and women, whereas the intake of thiamine decreased. In elderly men, the intake of protein, niacin, and vitamin D decreased, but the sugar intake increased.In elderly women, the cholesterol intake increased. The KHEI score was significantly lower in rural elderly men and women after the pandemic than before (male: 62.60 ± 0.56 for the post-COVID-19 and 64.76 ± 0.75 for the pre-COVID-19, p < 0.05; female: 64.07 ± 0.80 for the post-COVID-19 and 64.47 ± 0.62 for the post-COVID-19, p < 0.001). Regarding the KHEI component, in men, the intake of meat, fish, and legumes, all sources of protein, decreased significantly after the COVID-19 pandemic. In women, the total food intake and the intake of breakfast decreased significantly after the pandemic. In the moderation components, men were unable to limit their total sugar and sodium intake, while women were unable to control their saturated fatty acids and sodium intake. Furthermore, considering the total KHEI score, both elderly men and women had decreased values in the moderation and adequacy components following COVID-19. Conclusion These results provide foundational data for elderly nutrition policies or educational programs targeting the elderly population in rural areas in similar pandemic situations in the future.

Purpose: This study examined the nutritional status and Korean Healthy Eating Index (KHEI) scores pre- and post-coronavirus disease 2019 (COVID-19) outbreak in elderly men and women living in rural areas. Methods: The participants were 1,747 rural elderly people aged 65 or older who participated in the nutritional survey of the 2018–2021 Korea National Health and Nutrition Survey. The nutrient intake was estimated from a 24-hour recall. Results: Following the COVID pandemic, the intake of fat, saturated fatty acids, and sodium increased in elderly men and women, whereas the intake of thiamine decreased. In elderly men, the intake of protein, niacin, and vitamin D decreased, but the sugar intake increased.In elderly women, the cholesterol intake increased. The KHEI score was significantly lower in rural elderly men and women after the pandemic than before (male: 62.60 ± 0.56 for the post-COVID-19 and 64.76 ± 0.75 for the pre-COVID-19, p < 0.05; female: 64.07 ± 0.80 for the post-COVID-19 and 64.47 ± 0.62 for the post-COVID-19, p < 0.001). Regarding the KHEI component, in men, the intake of meat, fish, and legumes, all sources of protein, decreased significantly after the COVID-19 pandemic. In women, the total food intake and the intake of breakfast decreased significantly after the pandemic. In the moderation components, men were unable to limit their total sugar and sodium intake, while women were unable to control their saturated fatty acids and sodium intake. Furthermore, considering the total KHEI score, both elderly men and women had decreased values in the moderation and adequacy components following COVID-19. Conclusion These results provide foundational data for elderly nutrition policies or educational programs targeting the elderly population in rural areas in similar pandemic situations in the future.

BACKGROUND/OBJECTIVES: Accumulating evidence has shown the beneficial effects of isoflavone on health. There is limited information on the usual isoflavone intake for Koreans.This study examined the usual intake of total isoflavone and its major food sources in Koreans according to age and gender. SUBJECTS/METHODS: The dietary intake data of 21,271 participants aged 1 yrs and older from the Korea National Health and Nutrition Examination Survey (KNHANES) VII 2016– 2018 were analyzed. The average isoflavone intake was estimated based on the 24-h dietary recall data in KNHANES and the isoflavone database from the Korea Rural Development Administration (RDA) and literatures. The usual isoflavone intake was estimated by applying the ratio of within- and between-participant variance estimated from the 2009 KNHANES data to the 7th KNHANES (2016–2018) data. The variance of the isoflavone intake was calculated using MIXTRAN macro with intake data for two days in the 2009 KNHANES.Complex sample analysis with stratified variables and integrated weights was conducted. RESULTS: The mean total isoflavone intake in the Korean population aged 1 yrs and older (n = 21,271) was 139.27 mg/d, which was higher than the usual intake of 47.44mg/d. Legumes were a major contributing food group (91%), with arrowroot being a major individual contributor to the isoflavone intake (67.2%), followed by 21.3% of soybean, 5.4% of bean sprouts, and 2.1% of tofu. The usual isoflavone intake was highest in the participants aged 50 to 64 yrs old and increased with age until 50 to 64 yrs and then decreased with further increases in age.The usual isoflavone intake of participants aged 65 yrs and older was higher for men than for women, showing gender differences. CONCLUSIONS The usual dietary intake of isoflavone varied according to age and gender in the Korean population. This study showed that the usual isoflavone intake was lower than the average isoflavone intake. The difference between percentiles of the usual isoflavone intake was similarly smaller than the average intake. An estimation of average intake can be hindered by the occasional consumption of foods high in isoflavones, suggesting that the usual intake estimation method can be more appropriate. Further research will be needed to establish isoflavone dietary guidelines regarding the effects of isoflavone intake on health outcomes.

PURPOSE: To assess the effect of handwashing improving program and MRSA carrier detection program on MRSA(methicillin resistant Staphylococcus aureus) infection rate in a intensive care unit. METHOD: The intervention was Nosocomial Infection(NI) control program consisted of hand washing improving program and identification and treatment of MRSA carrier. Data on the NI and MRSA infections were collected by an infection control nurse based on the definition of CDC. MRSA infection rates were calculated by the number of MRSA infection per 100 admissions or 1,000 patients-days. The difference of MRSA infection rates between pre and post intervention was tested by Chi-square at =.05. RESULT: MRSA infection rates 3.0% or 3.2 per 1,000 patient-days at the pre, 4.6% or 3.7 per 1,000 patient-days at the post, and the differences were not statistically significant (p=.411, p=.769 respectively). CONCLUSION: The handwashing improving program and MRSA carrier detection program was not effective in reducing the Nosocomial Infection(NI) or MRSA infection rates. It is recommended further studies with a longer intervention and follow-up period.

BACKGROUND AND OBJECTIVES: Among the postoperative complications of Caldwell-Luc operation, maxillary cysts are sometimes difficult to manage properly by conventional revision Caldwell-Luc procedure. However, with the advent of endoscopic procedures, the technique of intranasal fenestration operation through the inferior meatus has been improved. This study evaluate the surgical results of inferior meatal fenestration operation and examines the clinical indication of this technique. The authors also review surgical cases of postoperative maxillary cyst. MATERIALS AND METHOD: We reviewed 58 cases of postoperative maxillary cyst which were treated surgically in the last 10 years. We analyzed their chief complaints such as sex, age, duration between the initial surgery and the presentation of the symptoms, symptom relief after revision surgery, and the recurrence rate of each surgical techniques. Sites of the cyst in the maxillary sinus and the existence of the septa were analysed radiologically by using computed tomograms of paranasal sinuses. Fourty-eight cases received inferior meatal fenestration operation and 12 cases received revision Caldwell-Luc operation. RESULTS: Inferior meatal fenestration operation could be best indicated in the patients with bulging cysts in the inferior meatus. However, the non-bulging cysts which tare in contact with the inferior metus and those with the inferior metus and those with incomplete septa were also treated with the inferior meatal fenestration operation without recurrence. Comparing with the revision Caldwel-Luc procedure, the inferior meatal fenestration operation was less time consuming and provided less postoperative discomfort for the patients. Also, since the operation is carried out under the direct endoscopic control, fine procedures and the removal of septa in the cysts were possible. CONCLUSIONS: For inferior meatal fenesration operation, the need for a pre-operative endoscopic nasal examination and an evaluation of comography should be emphasized before selecting the suitable method of treatment. Inferior meatal fenestration operations appear to be the most suitable treatment for cases where cysts bulge into the inferior meatus.
Humans ; Maxillary Sinus ; Paranasal Sinuses ; Postoperative Complications ; Recurrence

Background: Canine adipose-derived stem cells (cADSCs) exhibit various differentiation properties and are isolated from the canine subcutaneous fat. Although cADSCs are valuable as tools for research on adipogenic differentiation, studies focusing on adipogenic differentiation methods and the underlying mechanisms are still lacking. Objectives: In this study, we aimed to establish an optimal method for adipogenic differentiation conditions of cADSCs and evaluate the role of peroxisome proliferatoractivated receptor gamma (PPARγ) and estrogen receptor (ER) signaling in the adipogenic differentiation. Methods: To induce adipogenic differentiation of cADSCs, 3 different adipogenic medium conditions, MDI, DRI, and MDRI, using 3-isobutyl-1-methylxanthine (M), dexamethasone (D), insulin (I), and rosiglitazone (R) were tested. Results: MDRI, addition of PPARγ agonist rosiglitazone to MDI, was the most significantly facilitated cADSC into adipocyte. GW9662, an antagonist of PPARγ, significantly reduced adipogenic differentiation induced by rosiglitazone. Adipogenic differentiation was also stimulated when 17β-estradiol was added to MDI and DRI, and this stimulation was inhibited by the ER antagonist ICI182,780. Conclusions Taken together, our results suggest that PPARγ and ER signaling are related to the adipogenic differentiation of cADSCs. This study could provide basic information for future research on obesity or anti-obesity mechanisms in dogs.

Background: Canine adipose-derived stem cells (cADSCs) exhibit various differentiation properties and are isolated from the canine subcutaneous fat. Although cADSCs are valuable as tools for research on adipogenic differentiation, studies focusing on adipogenic differentiation methods and the underlying mechanisms are still lacking. Objectives: In this study, we aimed to establish an optimal method for adipogenic differentiation conditions of cADSCs and evaluate the role of peroxisome proliferatoractivated receptor gamma (PPARγ) and estrogen receptor (ER) signaling in the adipogenic differentiation. Methods: To induce adipogenic differentiation of cADSCs, 3 different adipogenic medium conditions, MDI, DRI, and MDRI, using 3-isobutyl-1-methylxanthine (M), dexamethasone (D), insulin (I), and rosiglitazone (R) were tested. Results: MDRI, addition of PPARγ agonist rosiglitazone to MDI, was the most significantly facilitated cADSC into adipocyte. GW9662, an antagonist of PPARγ, significantly reduced adipogenic differentiation induced by rosiglitazone. Adipogenic differentiation was also stimulated when 17β-estradiol was added to MDI and DRI, and this stimulation was inhibited by the ER antagonist ICI182,780. Conclusions Taken together, our results suggest that PPARγ and ER signaling are related to the adipogenic differentiation of cADSCs. This study could provide basic information for future research on obesity or anti-obesity mechanisms in dogs.

A clinical analysis was performed on 326 cases of the non-penetrating rib fractures experienced in the department of thoracic and cardiovascular surgery, Chosun University Hospital and 102 cases of the non-penetrating rib fractures those has been admitted and treated in the department of thoracic and cardiovascular surgery, Jeonnam Hwasoon Jung-ang Hospital during a period from Jan. 1994 to Dec. 1995. 1. Sex ratio was 3.4:1 in urban and 3.6:1 in rural communities with male predominance. 2. Most common cause were traffic accident in both communities. 3. Hemothorax, pneumothorax and hemopneumothorax which needed thoracostomy were observed in 146 cases(44.7%) in the urban and 12 cases(11.7%) in the rural communities. 4. Left thorax was the spell site of rib fractures in both communities. 5. Rib fracture was prevalent from 3rd to 6th rib in both communities. 6. Open thoracotomy was performed in 37 cases(11.3%) in urban and 3 cases(2.9%) in rural communities. 7. Overall mortality was 4.29%(14 cases) in urban area and, 1.96%(2 cases) in rural communities, and cause of death were hypovolemic shock, brain edema, sepsis, respiratory failure, asphyxia, and cardiogenic shock.
Accidents, Traffic ; Asphyxia ; Brain Edema ; Cause of Death ; Hemopneumothorax ; Hemothorax ; Humans ; Jeollanam-do ; Male ; Mortality ; Pneumothorax ; Respiratory Insufficiency ; Rib Fractures* ; Ribs* ; Rural Population* ; Sepsis ; Sex Ratio ; Shock ; Shock, Cardiogenic ; Thoracostomy ; Thoracotomy ; Thorax