1.Measurement of house dust mite-specific IgA antibody in sputum from asthmatics.
Korean Journal of Allergy 1997;17(1):34-34
To evaulate the possible pathogenetic significance of allergen-specific IgA antibody in respiratory secretion from asthmatics, we measured house dust mite(HDM)-specific IgA antibody in 3% saline-induced sputum from 23 HDM-sensitive asthmatics, 4 atopic asthmatics without mite-sensitivity, 6 non-atopic asthmatics, and 13 non-atopic, non-asthmatic controls (including 6 non-atopic healthy controls, 4 patients with chronic bronchitis, and 3 patients with rheumatoid arthritis) by ELISA. We also measured HDM-specific IgA antibody in serum and numbers of eosinophils in sputum. 1) Levels of HDM-specific IgA antibody in sputum from mite-sensitive asthmatics were significantly higher than those from non-atopic, non-asthmatic controls and non-atopic asthmatics(p<0.05). Levels of HDM-specific IgA antibody in sputum from atopic asthmatics without mite-sensitivity were significantly higher than those from non-atopic, non-asthmatic controls (p<0.05), however HDM-specific IgA/albumin raito was not significantly different between two groups (p>0.05). 2) The ratio of HDM-specific IgA antibody to albumin in sputum was not significantly different in mite-sensitive asthmatics with sputum eosinophila (> or = 5% of 200 counted leukocytes) and those without sputum eosinophilia (p>0.05). 3) The ratio of HDM-specific IgA to albumin in sputum from asthmatics was higher than that of serum. 4) There was no significant correlation of HDM-specific IgA/albumin ratios between serum and sputum (p>0.05). 5) When comparing sputum and saliva samples from 7 mite-sensitive asthmatics, levels of HDM-specific IgA antibody in sputum were significantly higher than those in saliva (p<0.05). In conclusion, HDM-specific IgA anti-body was increased in sputum from HDM-sensitive asthmatics, and it might be locally produced from bronchial mucosa. To evlauate the pathogenetic significance of allergen-specific IgA antibody in respiratory secretion from asthmatics, further studies might be needed.
Bronchitis, Chronic
;
Dust*
;
Enzyme-Linked Immunosorbent Assay
;
Eosinophilia
;
Eosinophils
;
Humans
;
Immunoglobulin A*
;
Mucous Membrane
;
Saliva
;
Sputum*
2.The rubber band ligation for bleeding hemorrhoids.
Journal of the Korean Society of Coloproctology 1991;7(1):51-56
No abstract available.
Hemorrhage*
;
Hemorrhoids*
;
Ligation*
;
Rubber*
3.A Case of Eccrine Spiradenoma.
Yoon Sung PARK ; Hae Eul LEE ; Dong Sik BANG
Korean Journal of Dermatology 1983;21(4):483-487
Eccrine spiradenoma is clinically characterized by a solitary, movable, tender noduie occurring most frequently on the ventral surfase of the upper body. A striking symptom is paroxysmal pain. Histopathologically, the encapsulated lobules of the tumor are composed of masses of basophilic cells, usually arranged in whorls, cords, and pseudogland.s. Within the whorls and pseudoglands, two types of celIs are notedperipheral intensely staining cells and central pale-staining cells. We describe herein a patient with an indolent eccrine spiradenoma, which had been diagnosed histopathologically.
Basophils
;
Humans
;
Strikes, Employee
4.Validation of measurement of house dust mite-specific IgE antibodies in serum using enzyme-linked immunosorbent assay.
Tak YOO ; Dong Ho NAHM ; Hae Sim PARK
Journal of Asthma, Allergy and Clinical Immunology 1999;19(2):200-207
BACKGROUND: Measurement of allergen-specific IgE antibodies using enzyme-linked immunosorbent assay (ELISA) has been developed and the results were shown to correlate well with those obtained by radioimmunoassay (RIA). However, consensus on the optirnal condition and data expression method for the measurement of allergen-specific IgE using ELISA is still not present. Object: To define the optimal condition for the measurement of allergen-specific IgE using ELISA and to evaluate the accuracy and reproducibility of the results, METHOD: We measured the concentrations of house dust mite-specific IgE antibodies in serum samples by ELISA and RIA method (AlaSTAT, DPC, USA) using standardized Dermatop~hagoides farinae antigen (kindly donated by Allergopharma Joachim Ganzer KG, Reinbek, Germany). RESULTS: Optirnal antigen coating amount was 2 ug/well and optimal serum dilution was 1: 10 for ELISA. The expression of the absolute concentration of house dust mite-specific IgE antibodies within the unknown sample using serial dilutions of samples and standard serum seemed to be more reasonable than the expression of absorbance value at a single serum dilution, because the former method provided better inter-assay variation and correlation with RIA results. The results of specific IgE rneasurement using ELISA significantly correlated with RIA results (r=0.96, p<0.001, n=26). CONCLUSION: These findings suggest that the measurement of allergen-specific IgE antibodies using the ELISA method can be accurate and reliable if optimal assay conditions and standardized data expression are applied.
Antibodies*
;
Consensus
;
Dust*
;
Enzyme-Linked Immunosorbent Assay*
;
Immunoglobulin E*
;
Pyroglyphidae
;
Radioimmunoassay
5.Evidence for neutrophil activation in induced sputum from patients with TDI - induced occupational asthma.
Hee Yeon KIM ; Dong Ho NAHM ; Hae Sim PARK
Journal of Asthma, Allergy and Clinical Immunology 1998;18(3):441-450
BACKGROUND: Toluene diisocyanate (TDI) is the most prevalent agent to cause occupational asthma (OA) in Korea. The pathogenic mechanism of TDI-induced OA is still unclear. Involvement of both immunological and non-immunologicaI mechanisms have been suggested. OBJECTIVE: To evaluate a possible role of neutrophil in the development of TDI-asthma. OBJECT AND METHOD: Myeloperoxidase (MPO) as a neutrophil activation marker in both serum and induced sputum, and IL-8 in induced sputum were measured. Induced sputa and sera were collected from 15 TDI-induced OA patients (classified to group I) during TDI- bronchoprovocation test and were compared with those from 11 asthmatic subjects with negative TDI-bronchoprovocation test (group II). MPO levels were measured by radioimmunoassay, IL-8 levels, by enzyme linked immunosorbent assay and albumin levels, by nephelometry. Sputum MPO and IL-8 levels were presented as a ratio to albumin. RESULT: Serum MPO level tended to decrease during the TDI-bronchoprovocation test in two groups, but no statistical significance was reached (p>0.05). However, the ratios of MPO (the ratio of MPO level measured at 30 min to MPO level at baseline, and the ratio MPO level measured at 360 min to MPO baseline) in group I were significantly lower than group II (p=0.004, p=0.03 respectively). The IL-8/albumin and MPO/albumin levels in induced sputum from group I were significantly increased after the TDI-bronchprovocation test in comparison to the baseline value which was obtained before the bronchoprovocation test (p=0.0l, p=0.02 respectively). There was a significant correlation between the percent increase of IL-8/albumin and the MPO/albumin in induced sputum (r=0.89, p<0.05). CONCLUSION: These findings suggest a possible involvement of neutrophil in the development of bronchoconstiction after the TDI exposure, and IL-8 might contribute to neutrophil recruitment to airway mucosa. Further investigation will be needed to investigate mechanism of neutrophil activation in the pathogenesis af TDI-induced OA.
Asthma, Occupational*
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-8
;
Korea
;
Mucous Membrane
;
Nephelometry and Turbidimetry
;
Neutrophil Activation*
;
Neutrophil Infiltration
;
Neutrophils*
;
Peroxidase
;
Radioimmunoassay
;
Sputum*
;
Toluene 2,4-Diisocyanate
6.Clinical features of bee venom anaphylaxis.
Sung Gyun AHN ; Sun Sin KIM ; Hee Yeon KIM ; Dong Ho NAHM ; Hae Sim PARK
Journal of Asthma, Allergy and Clinical Immunology 1999;19(3):492-497
Background and OBJECTIVE: There has been a few case reports of anaphylaxis due to honeybee in Korea. In order to observe the clinical feature of bee sting anaphylaxis. Moderials and methods: Six patients living in Kyunggi province area were referred under history of anaphylaxis after the bee sting. Atopy was defined by skin prick test result to common inhalant allergen. Serum specific IgE antibody to each bee antigen was detected by radioimmunoassay to identify the causative bee. RESULTS: All six cases were female. Three had atopy and four had combined allergic diseases such as allergic rhinitis, asthma, and urticaria. The etiologic bees consisted of yellow jacket (6 cases), paper wasp (4 cases), yellow hornet (3 cases), white faced hornet (1 case) and honey bee (1 case). Four cases had experienced anaphylaxis after ant bite and they showed positive result on specific IgE to imported fire ant. Specific immunotherapy against causative bee venom was begun using bee venom extracts from Bayer (USA) based upon results of specific IgE anti-body to bee venom. CONCLUSION: The yellow jacket is the most common cause of bee venom anaphylaxis in this area. Further studies will be needed to evaluate possible cross-reactivity between bee and ant venom.
Anaphylaxis*
;
Ant Venoms
;
Ants
;
Asthma
;
Bee Venoms*
;
Bees*
;
Bites and Stings
;
Female
;
Fires
;
Gyeonggi-do
;
Honey
;
Humans
;
Immunoglobulin E
;
Immunotherapy
;
Korea
;
Radioimmunoassay
;
Rhinitis
;
Skin
;
Urticaria
;
Wasps
7.Angioedema-like facial swelling as a first manifestation of systemic lupus erythematosus.
O Yeong KWON ; Chang Hee SEO ; Sung Taek JUNG ; Dong Ho NAHM ; Hae Sim PARK
Journal of Asthma, Allergy and Clinical Immunology 1998;18(1):90-95
Angioedma is a group of disorders with multifactorial etiology but a similar clinical expression, is characterized by swelling of subcutaneous or submucosal tissue. Systemic lupus erythematosus(SLE) is a chronic multi-systemtic disease characterized by inflammation and tissue damage resulting from deposition of auto-antibodies and immune complex. We experienced a case of angioedema which was the first manifestation of SLE in 24-year-old female patient. She had suffered from severe facial edema and multiple lymphadenopathy for six months and she also had pleural effusion, positive anti-nuclear and anti-DNA antibody test. Marked decrease of C3 and C4 levels was noted with normal antigenic level, and activity of Cl esterase inhibitor. The angioedema was not improved with anti-hitamine agents and instead disappeared with use of corticosteroid and non-steroidal anti-inflammatory drugs. Complement levels normalized after corticosteroid treament. We report a case of SLE which initial manifestation was angioedema.
Angioedema
;
Antigen-Antibody Complex
;
Complement System Proteins
;
Edema
;
Female
;
Humans
;
Inflammation
;
Lupus Erythematosus, Systemic*
;
Lymphatic Diseases
;
Pleural Effusion
;
Young Adult
8.Revival of Hop Japanese pollinosis in asthmatic subjects in Kyungki area.
Hae Sim PARK ; So Yeon COI ; Dong Ho NAHM ; Hee Yeon KIM
Journal of Asthma, Allergy and Clinical Immunology 1998;18(1):52-60
BACKGROUND: Hop Japanese (Hop J) pollens are abundant in the air of Korea during the autumn season. Their significances as a source of allergenic sensitization have been underestimated in this country. MATERIAL AND METHOD: In other to observe clinical features of Hop J-sensitive asthmatic patients in this country, skin prick test with Hop J pollen was performed. The serum specific IgE antibodies to Hop J pollen antigen were detected by enzyme linked immunosorbent assay (ELISA) in positive responders (>2+ of A/H ratio) on skin prick test. To confirm the respiratory sensitization, bronchoprovocation test was performed in 17 asthmatic patients sensitive to this pollen. RESULT: Ten asthmatic subjects showed a significant bronchoconstriction following the inhalation of Hop J pollen extract (6 early and 4 dual astmatic responses) and all of them had high serum specific IgE bindings, with minimal bindings in negative responders. They have suffered from seasonal aggravation of asthmatic symptoms with or without rhinitis, and/or conjunctivitis symptoms. The skin reactivity to Hop J had more than 5+ of A/H ratio on skin prick test in nine positive responders, whlie negative responders showed from 1+ to 3+ response. Moreover, four (40%) asthmatic subjects showed a positive response to only the Hop J pollen on skin prick test and an isolated positive asthmatic response to the Hop J bronchoprovocation test. CONCLUSION: We believe that the Hop J pollen should be considered as an allergen during the Autumn season, and thus included in skin test batteries in this area. Some labelled having intrinsic asthma or rhinitis might be sensitized to this pollen or other unknown allergens.
Allergens
;
Antibodies
;
Asian Continental Ancestry Group*
;
Asthma
;
Bronchoconstriction
;
Conjunctivitis
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Humulus*
;
Immunoglobulin E
;
Inhalation
;
Korea
;
Pollen
;
Rhinitis
;
Rhinitis, Allergic, Seasonal*
;
Seasons
;
Skin
;
Skin Tests
9.A case of Ritter's disease.
Young Kui LEE ; Eun Mi KIM ; Dong Rak CHOI ; Hae Ran LEE ; Chong Young PARK
Journal of the Korean Pediatric Society 1992;35(6):840-844
No abstract available.
Staphylococcal Scalded Skin Syndrome*
10.A Clinical Observation on the Nephrectomized Patient.
Korean Journal of Urology 1981;22(4):389-395
A clinical observation on the 120 patients nephrectomized in the Department of Urology, College of Medicine, Hanyang University, during the period from Mar 1972 to April 1980 was presented with a brief review of the literature. The results were as follows: 1. The total number of in-patients during above period was 1,881 and nephrectomies were performed in 120 of the 1,469 total urologic operations (8. 2%). 2. Age distribution showed the highest in 50`s with 20.8%, and the youngest was 2 year and the oldest 70 years. Sex distribution was almost equal. 3. The causative lesions of the kidney showed the highest in tuberculosis 38 cases (31. 6%), kidney 25 cases (20. 8%), tumor 16 cases (13. 4%), injury 12 cases (10. 0%), and chronic pyelonephritis 9 cases (7.6%) by sequence. 4. Subcostal approach was the most frequently used method in 54 cases (45. 0%), lumbar approach with the resection of the with rib was 28 cases (23. 3%). 5. The average amount of transfused whole blood of the transfused patients was 1,933cc in tumor, 1,700cc in injury, and total average amount was 1,113cc. 6. Postoperative complications included 7 cases of wound infection, 3 pneumothorax, 2 paralytic ileus, 1 bleeding. 7. 77 cases (61. 7%) were discharged within 10 days after surgery and only 2 cases(1. 6%) remained more than 1 month.
Age Distribution
;
Hemorrhage
;
Humans
;
Intestinal Pseudo-Obstruction
;
Kidney
;
Nephrectomy
;
Pneumothorax
;
Postoperative Complications
;
Pyelonephritis
;
Ribs
;
Sex Distribution
;
Tuberculosis
;
Urology
;
Wound Infection