The concentration of paracetamol in rabbit blood is determined by HPLC with UV detection at the wavelength of 257 nm and mobile phase of CH3OH-H2¬O-CH3COOH (20:79:1). We have determined the extent and rate of drug absorption into blood (through Cmax, Tmax and AUC calculated from the blood concentrations) and the extent and rate of dissolution (by the dissolution test mainly based on the paddle method described in USP 23) with 3 different finished products of paracetamol (by two Vietnamese manufacturers and by Smithkline Beecham) of the same dosage form (500 mg tablet). The test was carried out on 5 rabbits for each product and the results showed that there was no significant difference between the domestic and foreign drugs in both dissolution and absorption
Acetaminophen ; Biological Availability ; Pharmaceutical Preparations

Longitudinal study of 157 children from 14-18 weeks of gestation to 5 years after delivery to find out relationship between weight gain during pregnancy and birth weight with IQ index of children 5 years of old. Raven's test (Progressive Matrices) was used to evaluate mental development of child. The results showed that: there was positive correlation between birth weight and IQ index. The children with lower birth weight will have IQ index lower after 5 years delivery. In addition to, the results of study showed that: weight gain during pregnancy had strong positive correlation with birth weight
Pregnancy ; Weight Gain ; Birth Weight ; Child

Background: Currently, tracking, monitoring insecticide resistance of mosquitoes transmitting disease is an urgent need.\r\n', u'Objectives: To evaluate resistance to insecticide of three mosquito species: Aedes Aegypti, Culex quinquefasciatus and culex tritaenyorhynchus in Ha Noi\r\n', u'Subject and method: Aedes Aegypti, Culex quinquefasciatus culex tritaenyorhynchus mosquito species was collected from some districts of Ha Noi city. These mosquito species was tested sensitivity with 4 insecticides: permethrin 0.75%, lambda-cyhalothrin 0.05%, alpha-cypermethrin 30mg/m2 and DDT 4.0%.\r\n', u'Results: Three main vectors of Dengue fever, Japanese Encephalic and Filariasis mosquitoes were found in a very high density in some areas of Ha Noi City. Aedes aegypti mosquitoes in the districts of Thanh Xuan (TX), Dong Da (DD) and Hoang Mai (HM) was found highly resistant to permethrin, alpha - cypermethrin, lambda - cyhalothrin and DDT with the mortalities rates of 51 % (TX) and 59%(DD); 70%(HM); 90%, 82% and 96%; 74%, 54% and 59%; 1 %, 8% and 32%, respectively. \r\n', u'Culex tritaenyorhynchus was found also highly resistant to permethrin with the mortality rate of 29%, alphacypermethrin - 18%, lambdacyhalothrin - only 3% and DDT x36%. Culex quinquefasciatus was found also highly resistant to those insecticides. \r\n', u'Conclusion: Other insecticides should choose to replace the chemicals used in this experiment to control, kill mosquitoes when epidemics occur.\r\n', u'
Insecticides ; resistance ; Aedes Aegypti ; Culex quinquefasciatus ; culex tritaenyorhynchus

Background: Anti \ufffd?HLA (Human Leukocyte Antigen) antibody is result of immunization in allotransplantation. Organ transplantation is one of the great scientific achievements of the medicine. However, it is difficult to have the perfect harmony of HLA group. Inevitable consequence is the graft will be eliminated by the immune process. In Vietnam, organ transplantation was a relatively new specialty and there was not much research on evaluation immune process after transplantation. Objectives: To determine the rate of present of anti \ufffd?HLA antibody on transplant patients, and the role of post \ufffd?transplant anti \ufffd?HLA antibodies on long \ufffd?term graft function. Subjects and method: ELISA technique was used to analysis 31 blood samples of 31 patients who were transplanted organs at 103 military hospital and Cho Ray hospital from May 2000 to July 2007. This was a retrospective and described cross-sectional study on theclinical records. Results:The rate of anti \ufffd?HLA antibodies was 35.5%. The present of anti \ufffd?HLA antibodies of transplant patients had negative impact on graft function. Conclusion: The detection of anti \ufffd?HLA antibodies by ELISA in the post transplant period may be a high confident and sensitive technique for follows up graft function.
Organ Transplantation/ contraindications ; HLA Antigens ; Antibodies ; Enzyme-Linked Immunosorbent Assay ;

Background: Metabolic syndrome is characterized by a group of metabolic risk factors in one person but not a real disease. For this reason, metabolic syndrome is underestimated by clinicians and individuals. Metabolic syndrome that was not detected and treated timely can cause serious diseases. Objectives: 1) To investigate the abnormal blood biochemical parameters related to metabolic syndrome. 2) To determine the abnormal ratio of these parameters. 3) To screen the metabolic syndrome among the staffs of Ha Noi Medical University. Subjects and method: 229 staffs underwent laboratory tests to diagnose for the metabolic syndrome. Abnormal blood biochemical parameters were defined as follows: Fasting blood glucose level increased 6.1mmol/l or more. Triglycerides level is 2.3mmol/l or more, HDL-cholesterol decreased to less than 1.03mmol/l for men and less than 1.24mmol/l for women. Total cholesterol level is 5.2mmol/l or more. CT to HDL-C ratio is more than 3.63 and LDL cholesterol level is 3.5mmol/l or more. Results. Rate of subjects with glucose intolerance or blood fasting glucose higher than 6.1mmol/L is 20.09%. Rate of subjects with dyslipidemia is 61.14%. Average levels of blood glucose, triglycerides and cholesterol increase with age. Number of staffs of Ha Noi Medical University have metabolic syndrome in 2007 are 32 persons (14%). M/F rate is equal and most common in persons over 45 years of age. Conclusion:There are significant number of staffs at the Ha Noi Medical University that had abnormal blood glucose and cholesterol levels related to metabolic syndrome.
Metabolic syndrome ; blood biochemical parameters.

Background: Dyslipidemia is an important risk factor of atherosclerosis. The endothelia can be injured by plaque that advantages for platelet adheadsion, aggregation and produces thrombosis. Objectives: (1) To evaluate the changes of platelet aggregation induced by ADP and collagen in hyperlipidemic patients; (2) To identify the relationship between platelet aggregation and various types of dyslipidemia. Subjects and methods: A descriptive cross sectional non-randomised control trial method was employed in this study. A total of 128 hyperlipidemic patients (in which 40 patients were type IIa, 36 were type lib, and 52 were type IV), and a control group of 88 people were measured the aggregation in platelet - rich plasma (PRP) induced by ADP and collagen. Results: There was no significant difference about the platelet counts in both intervention and control groups (p>0.05). ADP and collagen induced platelet aggregation increased significantly (p<0.01) in intervention group when compared to control group. A significant correlation was found between the plasma triglyceride concentration and platelet aggregation induced by ADP and collagen with r = 0,335 and 0,332 respectively, while that of cholesterol and ADP was 0,304. Conclusion: Platelet aggregation increased in hyperlipidemic patients and had a significant correlation with plasma triglyceride concentration. \r\n', u'\r\n', u'
Dyslipidemias/ pathology ; therapy ; Platelet Aggregation

Background: Identifying the factors which affect the invasion and metastasis process takes a vital role in the treatment of cancer. This is the first study on the relationship between the expression of matrix metalloproteinase (MMP \u2013 9), invasion and metastasis of nasopharyngeal carcinoma (NPC) in Vietnam. Objectives: To evaluate the significance of matrix metalloproteinase \u2013 9 in predicting the metastasis potential of NPC. Subject and Method: The expression of MMP \u2013 9 was studied by immunohistochemical analysis in 33 nasopharyngeal carcinoma sections and the associations of MMP-9 with lymph nodes metastasis and clinical features. Results: Positive immunostaining for MMP-9 was observed in 94.94% of the NPCs, 39.4% of the cases being extensively positive (+++). The expression of MMP-9 correlated significantly with cervical lymph nodes metastasis (p<0.05). 5/16 cases (31.25% of the cases) without cervical lymph nodes metastasis expressed MMP-9 with 2+ or 3+ level, non-significant of MMP-9 expression with T classification and clinical stage. Conclusions: The expression of MMP-9 often goes along with lymph nodes metastasis in nasopharyngeal carcinoma.
MMP-9 ; Nasopharyngeal carcinoma

Background: Statin and its derivate (simvastatin, autorvastatin, etc...) are used for dyslipidemia treatment and preventing thrombose. However, the mechanism of the antithrombotic action is still being studied. Objectives: (1) To study coagulation parameters in dyslipidemia. (2) To evaluate the effects of simvastatin on coagulation parameters in dyslipidemia patients. Subject and Method: A prospective study was carried out in a sample of 22 patients with primary hypercholesterolemia (type IIa), who were treated with simvastatin 20mg/d for 1 month. The lipid parameters (cholesterol, triglycerid, HDL, LDL) and coagulation parameters (PT, APTT, fibrinogen, factor II, V, VII, X, VIII, IX, XI) were compared between pre and post therapy, and to the control group (59 healthy people). Results: Most of coagulation parameter values (except factor VIII and X) of the pre treatment group were significantly change towards hypercoagulation (p<0.05%) when compared to the control group. After treatment, PT rate, APTT, APTT rate, fibrinogen, factor VII and IX were significantly changed towards coagulation when compared to pre treatment (p<0.05%). The plasma coagulation and lipid parameters of more than 50% of the hypercholesterolemia patients returned to normal values after treatment. Conclusions: Simvastatin therapy on dyslipidemia patients can reduce not only the level of serum lipid, but also coagulation, and proved its effectiveness in the prevention of thrombosis.
Simvastatin ; coagulation ; dyslipidemia

Introduction and Purpose
Overweight and obesity have recently emerged as major problems of malnutrition among children in developing countries. However, risk factors for childhood obesity in Asian countries remain largely unknown. Identification of target populations for its prevention and intervention is urgently needed. This survey was conducted to assess the prevalence of overweight and obesity and identify possible risk factors among kindergarten children in Ho Chi Minh City, Vietnam.
Methods
In this cross-sectional study conducted in March, 2005, 780 children in their third and fourth years in kindergarten and their mothers were randomly selected from eight local kindergartens. Height and weight of mother-child pairs were measured, and an interview was conducted by trained staff using a questionnaire in Vietnamese. Overweight/obesity was defined as a weight-for-height > 2 SD above the National Center for Health Statistics median reference value. Risk factors for overweight/obesity were analyzed using logistic regression.
Results
There were 741 respondents (377 boys and 364 girls); the response rate was 95.0%. Average age of children was 61.8 ± 6.8 months; the average age of mothers was 35.0 ± 5.2 years. The average height and weight were 110 ± 5.7cm and 21.2 ± 4.0kg respectively. Of the 741 children, 27.8% were identified as being overweight/obesity. Four possible variables selected were: 1) child's sex, 2) mother's awareness of her child's current body type, 3) mother's expectation for her child's body type in the future, 4) mother's awareness of her own current body type .
Conclusion
Our findings point to the importance of addressing appropriate body images in the management of children's nutritional health. Providing sufficient amounts of information on appropriate body type in addition to feeding practice to mothers should be a priority at the Ho Chi Minh City Nutrition Center to prevent child overweight and obesity.

Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC 50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.