Objective:To investigate the relationship between the expression of secreted protein acidic and rich in cysteine (SPARC)and the glycolysis in hepatocarcinoma cells.Methods:HepG2 hepatocarcinoma cells were transfected with SPARC plasmid and SPARC siRNA,respectively.The change of glucose uptake and lactate generation after transfection was assayed by colourimetry method.Results:The glucose uptake and lactate generation decreased in HepG2 transfected with SPARC plas-mid,compared with the control group (P <0.05).However,the glucose uptake and lactate generation increased in HepG2 transfected with siRNA SPARC,compared with the control group(P <0.05).Conclusions:SPARC negatively regulates gly-colysis in hepatocarcinoma cells.