Five purple non-sulfur bacterial strains were isolated from the sediments of the rearing pond. According to the cultural, morphological, cellular spectra, physiological properties and inner intracytoplasmic membrane structures, strain AS-31 that was short rod and contained lamellar intracytoplasmic membrane was identified as Rhodopseudomonas palustris, and other four isolates (AS-32, DA-32, SY-32, and SY34) which were spheroid and contained vesicular intracytoplasmic membranes were identified as Rhodobacter spheroids. Pure cultures of strains AS-31 and SY-32 were used as additive in aquiculture of Tilapia sp.. After weeks of incubation, the weight and surviving percentage of experimental fishes increased about 20% than those of the control group without any additive. However, the mixed culture of strains AS-31 and SY32 have no obvious effect than that of the pure one. It demonstrated that the cultures of purple non-sulfur bacteria are of benefit to aquiculture and could be used as an additive.

OBJECTIVE To evaluate the changes in serum levels of TNF-? and IL-6 after thoracic operation,and the relationship with multiple organ dysfunction syndrome(MODS).METHODS Serum levels of TNF-? and IL-6 in 46 cases of benign disease between 2000 to 2003 were observed on the day of operation,the 3rd,7th,14th and 21th days after operation,the patients were divided into two groups according to the presence of MODS.RESULTS Cases occurred MODS in two weeks after thoracic operation,the serum levels of TNF-? and IL-6 were markedly higher in MODS than that in non-MODS,the correlation coefficients between them were r=0.9145,P

Liver functional reserve which functioned as the major evaluation factor for the tolerance of the liver surgery or the liver trauma had already been used wildly in preoperative evaluation for liver surgery.So far there were plenty of techniques for liver functional reserve.But each technique had its own advantages and disadvantages.This article briefly discussed the most wildly used techniques for liver functional reserve in preoperative evaluation.

Objective:To research the expense distribution of discharged patients during hospitalization to find out the period with the greatest weight, and provide reliable evidence for the increase of turnover rate by controlling hospitalization of patients and medical security. Methods:Totally 2 444 discharged patients of our hospital from September to November in 2013 with stay day of 1 to 20 d were analyzed, and then divided into the internal medicine group and surgical groups including surgical operation group and surgical without operation group. The hospitalization expenses and drug expense in all the groups were counted and compared. Results: The daily expense in the internal medicine group showed significant difference when compared with that in the surgical operation group and surgical without operation group (P<0. 01), meanwhile, that in the two surgical groups had no significant difference (P>0. 05). The daily expense in the surgical operation group was with linear decrease, and that in the other groups was with logarithmic decline. The 10-day expense in each group had significant difference (P<0. 05) compared by median test. The expense proportion of drugs in all groups was generally increased along with the hospitalization days. Conclusion:The effective method for the decrease of drug pro-portion is shortening hospitalization days,especially in surgical departmens,the drug proportion is increased along with the length of stay.

AIM:To study the effect of levofloxacin(LVFX)on serum concentrations and pharmacokinetics of phenytoin sodium(PHT)in rabbits.METHODS:Using UV-spectrophotometry,the serum concentrations of PHT were determined in nine rabbits before oral feeding LVFS and after a single dose of PHT given iv 10 days later.The pharmacokinetic parameters were worked out with 3p87 program.RESULTS:The concentration-time curve of PHT was fit to open two-compartment model.After co-administration of LVFX,the serum concentrations of PHT were decreased to some extent and part of pharmacokinetic parameters obviously changed as well.CONCLUSION:Co-administration of PHT and LVFX will accelerate the metabolic process of PHT.

Objective To investigate the causer of, and the way to deal with the measurement of protecting from vascular compromise in free flap transplantation. Methods To retrospectively study the clinical data collected from the 89th Hospital of PLA including 305 cases of vascular compromise in free flap transplantation held on June, 1983 to December, 2006. Accoding to the different tissue flaps and different sites to be repaired, 11 factors of vascular compromise of free flap transplantation were to be investigated including operation design, the recipe of free flap, the variation of vascellum, the skill of recipe, the operation opportunity, the match of vascellum, the debride of vascellum, the vascellum angiotasis, the vascular anastomosis, the management of vascular articulo and the infection. Results Among the 305 cases of free flap transplantation, arterial compromise existed in 270 cases, 10 cases developed arterial compromise during operation, 6 cases were due to the injury of nutrient vessel, the flap survived after the revascularization. 4 cases obtained part necrosis of 10 cases, and the rotation flap and skingrafting covered the raw surface. The rest 213 cases survived and 47 cased failed. Another 35 cases of vein compromise were obtained. Among them, 5 cases survived, part necrosis of 10 cases, and 20 cases failed. Conclusion Vascular compromise is the factor of necrosis in free flap transplantation. It is of cardinal importance to timely and correctly treat the vascular compromise in free flap transplantation whether happened intra-or postoperatively. This is the key to access high successful rate. Preventive measures are even more beneficial than proper management after its occurrence. The venous return disorder was the main factor of free flap transplantation failure.

Objective: To explore the change of activities of the cardiac autonomic nervous system in the EMG biofeedback with the quantified index of heart rate variability(HRV). Methods: Various physiological signals such as ECG、EEG、EMG were recorded by Synchronous Multi-Signal Recording Biofeedback System, and HRV analysis was applied to the extracted ECG signals. Thirty healthy young men received the tests. Results:Compared with the resting state, when the subjects entered biofeedback state, the values of low frequency power, indicator of the sympathetic activity decreased while the values of high frequency power (indicator of the vagal activity) increased, and the values of low frequency power/high frequency power ratio, the marker of the sympatho-vagal balance decreased too. During the later treatment sessions (after 7-10 times treatments) the change exhibited statistical difference and achieved one stable level (low frequency power, baseline 3.98?0.21, 3.82?0.15, 3.93?0.16, 3.91?0.23, test state 3.55?0.32, 3.51?0.29, 3.93?0.16, 3.39?0.26, t=-6.85～-3.68,P

BACKGROUND: Dynamic balance of endothelin (ET) and calcitonin gene-related peptide (CGRP) plays an important role in occurrence and development of cerebral infarction.OBJECTIVE: To observe the effects of thromboiytic therapy with batroxobin(DF-521) on the levels of plasma ET and CGRP after cerebral infarction.DESIGN: Randomized controlled study.SETTING: Fourth Department of Gereology, General Hospital of Guangzhou Military Area Command of Chinese PLA.MATERIALS: The experiment was performed at the Fourth Department of Gereology, General Hospital of Guangzhou Military Area Command of Chinese PLA. Totally 15 New Zealand rabbits aged from 18-20 months, of common grade and either sex, with the mean body mass of (3.5±0.5) kg were selected.METHODS: A total of 15 rabbits were established into cerebral infarction models with modified autogeneic thrombus method. One rat died when selecting models. Other 14 rats were randomly assigned into control group and treatment group. DF-521 was slowly injected into vein of ear edge of rats in the treatment group at hour 2 after embolism. Saline of the same volume was slowly injected into rats of the control group. Changes of concentrations of plasma ET and CGRP in the treatment group and control group were observed before and after cerebral infarction.MAIN OUTCOME MEASURES: Changes of ET and CGRP contents 1 hour before operation, 2 hours after operation (before drug injection) and 6 hours after operation (4 hours after drug injection).RESULTS: Of the 15 included rabbits, 1 rat died when establishing model, so 14 rabbits were involved in the result analysis. ET level significantly increased at hour 2 after acute cerebral infarction operation in the treatment group and control group (P ＜ 0.05, P ＜ 0.01), and then decreased at hour 6 after operation (P ＜ 0.01 ,P ＜ 0.01 ). The decrease in the treatment group was larger than that in the control group (P ＜ 0.01 ). The level of CGRP markedly reduced at hour 2 after acute cerebral infarction operation in the two groups, and than increased at hour 6 after operation. The increase in the treatment group was larger than that in the control group, but there was no statistical difference (P ＞ 0.05).CONCLUSION: Body with cerebral infarction during acute stage has selfregulative mechanism on dysequilibrium of ET and CGRP. Early-used thrombolytic therapy with DF-521 can markedly increase the ET level,which may be one of the protective mechanisms of cerebral ischemical reperfusion injury.

The research findings published by National Surgical Adjuvant Breast and Bowel Project have continued to change the clinical practice of early-stage breast cancer over the past decade.Sentinel lymph node biopsy has become the new surgical operation standard.The aromatase inhibitor adjuvant endocrine therapy cycle has been extended from 5 years to 10 years, which benefits the patients with high risk and reactivity.Chemotherapy combined with trastuzumab has become the standard adjuvant therapy strategy of human epidermal growth factor receptor-2-positive invasive breast cancer.In addition, the research progress is also related to the optimization of the fields including the postoperative adjuvant chemotherapy and neoadjuvant chemotherapy.

[Objective]To study the methods and opportunity of operation for the malignant tumor intercurrent fracture of extremity.[Method]Seventeen cases of malignant tumor intercurrent fracture of extremity were reviewed in our hospital from June 1996 to October 2003 in the study,which included osteosareoma 4 cases,giant cell tumor 3 cases,ewing tumor 1 case,ametoblastoma of bone Ⅰ case,bony metastases 8 eases.4 cases treated with exterior fixation and chemotherapy in Ⅰ P(period)and reconstruction alter tumor cutting in ⅡP(period),11 cases treated tumor eliminate and reconstruction in Ⅰ P(period).One case amputed in Ⅰ P(period)and 1 case amputed in Ⅱ P(period).[Result]All patients treated had been followed up from 12 to 88 months.One case occurred local rccurrrenee.and 2 cases died because of lung metastases in the 9 cases of primary malignant tumor.While 1 case occurred local reecurrenee and 4 cases died in the 8 cases of metastasis malignant tumor.[Conclusion]For the primary,malignant tumors with fracture.which were sensitive to chemotherapy,should first take into account to exterior fixation and chemotherapy inⅠ P(period)and reconstruction after tumor cutting in ⅡP(period),while for the insensitive giant cell tumor and bony metastases with fracture should be treated with tumor eliminate and reconstruction in ⅠP(period).