Angiotensin-converting enzyme 2 (ACE2)-angiotensin (1-7) [Ang (1-7)]-Mas constitutes the vasoprotective axis and is demon-strated to antagonize the vascular pathophysiological effects of the classical renin -angiotensin system .We hypothesize that upregulation of ACE2-Ang (1-7) signaling protects endothelial function through reducing oxidative stress , thus resulting in beneficial outcome in di-abetes.Ex vivo treatment with Ang (1-7) augmented endothelium-dependent relaxation (EDR) in renal arteries from diabetic patients . Both Ang (1-7) infusion via osmotic pump (500 ng? kg -1? min-1 ) for 2 weeks and exogenous ACE 2 overexpression mediated by ad-enoviral ACE2 via tail vein injection rescued the impaired EDR and flow-mediated dilatation ( FMD) in db/db mice.Diminazene acetu-rate treatment (15 mg? kg-1? d-1 ) activated ACE2, increased the circulating Ang (1-7) level, and augmented EDR and FMD in db/db mouse arteries.In addition, activation of the ACE2-Ang (1-7) axis reduced reactive oxygen species (ROS) overproduction de-termined by dihydroethidium staining , CM-H2DCFDA fluorescence imaging , and chemiluminescence assay in db/db mouse aortas and also in high-glucose-treated endothelial cells .Pharmacological benefits of ACE 2-Ang ( 1-7 ) upregulation on endothelial function were confirmed in ACE2 knockout mice both ex vivo and in vitro.We elucidate that the ACE2-Ang (1-7)-Mas axis serves as an important signal pathway in endothelial cell protection in diabetic mice , especially in diabetic human arteries .In summary, endogenous ACE2-Ang (1-7) activation or ACE2 overexpression preserves endothelial function in diabetic mice through increasing nitric oxide bioavail -ability and inhibiting oxidative stress , suggesting the therapeutic potential of ACE 2-Ang(1-7) axis activation against diabetic vasculop-athy.

Extranodal natural killer (NK)/T-cell lymphoma, nasal type (ENKTL) is an aggressive non-Hodgkin lymphoma and there are no standard treatment options for ENKTL. For localized patients, radiotherapy alone or radiotherapy combined with chemotherapy could be took into consideration. Systemic combination chemotherapy remains the primary treatment for patients with advanced ENKTL. The efficacy of anthracycline based conventional chemotherapy in ENKTL is unfavorable. This paper reviews the treatment progress of ENKTL in recent years.

Relations between doctor and patient are basic and important human relation in medical practice.Because of some reasons,the relations appear tense status in our country,moreover the market economy flourishingly develops,as the result of medical organizations suffering more and more existent pressure.It is an important subject faced for people,especially for medical worker,to righly think the existed defects,and set up the mutual action between doctor and patient.

CCN1 is a novel extracellular matrix-associated signaling protein which possesses 381 amino-acid residues to compose 4 distinct structural modules with 38 conserved cysteine residues.This protein has a variety of properties in cardiovascular system,affecting the cellular behaviors such as differentiation,proliferation and migration of vascular endothelial cells,smooth muscle cells and cardiac myocytes,that suggested an essential roles of CCN1 in angiogensis,vascular injury,cardiac development and myocardial infarction.

NMDA(N-Methyl-D-Aspartate)receptor is a kind of ionotropic receptors, which have been widely localized in peripheral somatic tissues and visceral pain pathways. The activation or expression changes of peripheral NMDA receptor may play an important role in pain occurrence. Peripheral administration of NMDA receptor antagonist can alleviate or prevent pain and enhance the analgesic effect of opiate. This method of administration can reduce the side effect of central action induced by the drug. It will be an important research direction of pain management.

Aim To observe effects of compound dialysate of Chinese herbs and Western medicines on small intestinal mucosa ultrafiltrative function in dogs. Methods Twenty Beagle dogs were divided into 4 groups: NS, control (CG), lower ligustrazine dose (LLDG) and higher ligustrazine dose group (HLDG). According to the arteriovenous region of small intestine mesentery to make three isolation loops for comparative study, they were respectively jejunum loop (JL), jejunoileum loop (JIL) and ileum loop(IL), and input hypersonic sample solution through vein after blocking the renal arteries to make the man-made renal failure models such as electrolytic disorder, azotemia etc. Meanwhile, the dialysate was perfused from the inlet of these isolation loops, respectively.Finally, these secretion of small intestine loops and blood samples which arteria and venae of the small intestine mesentery every 30 minutes were collected to determine the density of K+, Na+, Cl-, UN, CR, UA and the clearance and filtration rate in each group were calculated. Results The clearance rate and filtration rate in LLDG were remarkablely higher than that in the LHDG、the CG and the NS (P

Aim To explore the effect of the morphine(MP) on the Kv channel in bronchial smooth muscle in guinea-pigs (GPBSM) and expressions of the Kv 1.5 mRNA and protein . Methods The effect of the MP on the Kv channel was studied by patch clamp recording in GPBSM and expressions of the Kv 1.5 mRNA and protein were measured by Western-blot and RT-PCR techniques. Results Morphine remarkably restrained the Kv channel current and the expression of the Kv 1.5 mRNA and protein in cultured GPBSM (n=6, each group, P

A crystal with colourless and stick-form,C_(15)H_(26)N_2(M. W. 234, m. p.106), has been separated from the alcohol extract of leaves of Ammopiptanthus Mongolicus Maxim. It has been ascertained to be biguinalizidine alkaloid on the basis of its spectrum data and its structure was elucidated as L-?-isosparteine.

AIM: To establish HPLC method for the determination of berberine hydrochloride in Huanglianshangqing Tablets(Rhizoma Coptidis, Radix et Rhizoma Rhei, Fructus Forsythiae, etc.). METHODS: The Diamonsil TM C 18(20cm?4.6mm,5?m) was used with 0.033mol?L -1 Potassium dihydrogen phosphate solution-acetonitrile (70∶30) as a mobile phase. The flow rate was 1.0 mL?min -1. The detecting wavelength was 265nm. RESULTS: The linear of berberine hydrochloride was in the range of 0.2376～ 0.5544?g, r= 0.9999. The average recovery was 98.7% and RSD was 0.7%(n=5), respectively. CONCLUSION: The method is simple,rapid and accurate.

Inflammatory bowel disease(IBD)is a chronic nonspecific intestinal inflammatory disease and the etiology and pathogenesis are not fully clarified. Studies showed that immune factors play an important role in the pathogenesis of IBD. Cytapheresis(CAP)is a new blood purification method which can selectively filter or adsorb the inflammatory cells and cytokines in systemic circulation. Many studies confirmed that CAP had some therapeutic effect on IBD. This article reviewed the advances in study on CAP in treatment of IBD.