Objective To provide evidence for the prevention and control of nosocomial infection by knowing the infecting status of hospital.Methods The clinical data of 64 431 hospital patients from 2010 to 2012 were analyzed prospectively and retrospectively.Results A total of 2 636 patients of nosocomial infection were collected in 3 years,the infection rate was 4.09 ％ (2 636/64 431).Upper and lower respiratory tract was the main infection site.ICU,urology,neurology,endocrinology were the main infection outbreak departments.Gram negative bacilli was still the chief bacterium,accounted for 52.48％(689/1 313).Conclusion To strengthen the control of nosocomial infection and training of medical staff,strict insist on aseptic operation,reduce invasiveness operation,use antibacterial agent reasonable are the effective and preventative measures to decrease nosocomial infection.

Background and objectiveIncidence and mortality rates of lung cancer are rising sharply. Small cell lung cancer patients prefer chemotherapy than surgery because of the insigniifcant side effects of Chinese medicine. Studies have shown that solasonine possesses an anti-tumor property. hTe aim of this study is to investigate the effect of solasonine on the apoptosis of lung cancer cell line H446.MethodsAppropriate concentration and time were selected with a CCK8 kit. hTe drug that was used in H446 cells was divided into four different concentrations: 0 μmol/L, 3.4 μmol/L, 6.8 μmol/L and 13.6 μmol/L. hTe changes in forms and nucleus of H446 cells that were stained with DAPI were observed under an inverted optical microscope. hTe effects on H446 cell apoptosis were detected by FCM. hTe changes in apoptosis-related proteins BCL2, BAX, and CASP3 were investigated using Western blot for 24 h.Results Solasonine reduced the survival ratio of H446 cells and in-hibited the proliferation with a dose-related effect. hTe survival ratio of H446 cells could be reduced to 16.77% (P<0.001), and the highest apoptosis ratio was 44.62% (P<0.001). Apoptosis was observed in H446 cells. Moreover, Western blot showed that the apoptosis-related proteins BAX and CASP3 were upregulated (P<0.05).ConclusionhTe proliferation of H446 cells can be inhibited by solasonine, and the expression of pro-apoptotic proteins is up-regulated, and the expression of anti-apoptotic proteins is down-regulated, thereby promoting the apoptosis of cells.

Objective:To screen the factors influencing overall survival (OS) of patients undergoing radical resection for colorectal cancer (CRC) and to construct a prognostic model for OS of patients after CRC.Methods:The clinical data of 350 patients with stage Ⅰ-Ⅳ CRC who underwent radical resection in the People's Hospital of Wuhan University from March 2017 to December 2019 were collected retrospectively. Patients were divided into subgroups 0 ( n=70), 1 ( n=172), and 2 ( n=108) according to different preoperative systemic inflammation score (SIS). The relationship between different SIS, neutrophil to lymphocyte ratio (NLR), lymphocyte to monocyte ratio (LMR), systemic immune inflammation index (SII) and prognosis of CRC patients undergoing radical surgical resection were analyzed, and Cox regression models were used to perform univariate and multifactorial analyses of factors affecting patient prognosis, and column line graph models were constructed based on the results of multifactorial analyses. Results:By the deadline of follow-up, 80 of 350 CRC patients died, and the 5-year OS rate was 77.14%. The 5-year survival rates of patients in SIS group 0, group 1 and group 2 were 95.71%, 79.65% and 61.11% respectively, with a statistically significant difference ( χ2=30.19, P<0.001). Statistically significant differences in age ( χ2=19.40, P<0.001), tumor site ( χ2=8.18, P=0.017), T stage ( χ2=10.01, P=0.007), TNM stage ( χ2=14.80, P=0.001), tumor diameter ( χ2=13.91, P=0.001) and carcino-embryonic antigen (CEA) level ( χ2=10.12, P=0.006) among patients in SIS group 0, group 1 and group 2. The 5-year OS rates of patients in the low NLR and high NLR groups were 82.67% and 56.16% respectively, with a statistically significant difference ( χ2=24.96, P<0.001) ; the 5-year OS rates of patients in the low LMR and high LMR groups were 66.85% and 88.17% respectively, with a statistically significant difference ( χ2=22.45, P<0.001) ; the 5-year OS rates of patients in the low SII and high SII groups were 86.14% and 69.02% respectively, with a statistically significant difference ( χ2=14.76, P<0.001). Univariate analysis showed that age ( HR=2.58, 95% CI: 1.54-4.32, P<0.001), T stage ( HR=2.41, 95% CI: 1.24-4.68, P=0.009), N stage ( HR=3.03, 95% CI: 1.85-4.94, P<0.001), TNM stage ( HR=3.61, 95% CI: 2.15-6.04, P<0.001), nerve invasion ( HR=1.97, 95% CI: 1.27-3.08, P=0.002), vascular invasion ( HR=2.31, 95% CI: 1.49-3.59, P<0.001), preoperative SIS 1 score ( HR=5.09, 95% CI: 1.57-16.56, P=0.007), SIS 2 score ( HR=11.05, 95% CI: 3.42-35.65, P<0.001), NLR ( HR=2.97, 95% CI: 1.90-4.64, P<0.001), LMR ( HR=0.31, 95% CI: 0.19-0.52, P<0.001), and SII ( HR=2.50, 95% CI: 1.54-4.06, P<0.001) were all independent influence factors affecting the postoperative prognosis of CRC patients undergoing radical surgical resection; multivariate analysis showed that age >60 years ( HR=2.27, 95% CI: 1.31-3.91, P=0.003), TNM stage Ⅲ-Ⅳ ( HR=7.08, 95% CI: 1.89-26.59, P=0.004), and preoperative SIS 2 score ( HR=4.02, 95% CI: 1.09-14.83, P=0.037) were all independent risk factors affecting the postoperative prognosis of CRC patients undergoing radical surgical resection. The nomogram model built based on the screened variables has high prediction accuracy: the C-index of the nomogram was 0.75. Conclusion:Age>60 years old, TNM stage Ⅲ-Ⅳ, SIS 2 score are all independent risk factors for postoperative prognosis of colorectal cancer. The nomograph model constructed by this method has high prediction accuracy.