Objective: To explore the epidemiological characteristics of injury among middle school students in Zhongshan city, Guangdong Province, and to provide evidence for appropriate student injury prevention strategies and injury monitoring programs. Methods: The cluster random sampling method was used to collect injury information among 2 212 middle school students in urban and suburban areas of Zhongshan city. SPSS 22.0 was used for statistical analysis. Results: Among all the 2 212 middle school students, the incidence of injury was 17.95%. The incidence of injuries (20.9%)and mutiple injuries(6.67%)among boys was higher than that of girls(15.42%,4.61%),and the differences were of statistical significance(χ2=11.21,4.45,P<0.05). The incidence of multiple injuries among junior high school students(6.70%) was higher than that among high school students(4.48%)(χ2=5.20，P<0.05). The injury occurred in the school (63.76%) was higher than that occurred outside of the school (36.24%). Most injures were more likely to occur in sports area in school(33.16%),and most injuries were found occured while doing physical activities(38.01%). Conclusion The prevention of injury among middle school students should focus on junior high school students and male students. School-based injury prevention and health education should focus on campus sports-related injury.