OBJECTIVE To analyze the incidence and relevant factors of hospital infection of newborn baby in a mother with own baby room,to put forward the corresponding nursing countermeasures,as a result,and to probe into the nursing ways for decreasing the incidence of hospital infection of newborn baby in the same conditions.METHODS A retrospective analysis was made on the monitoring material concerning the hospital infection of newborn babies,who were born in our hospital from 2001 to 2005,in a mother with own baby room.RESULTS The average sick rate of hospital infection of newborn baby in the same conditions was 1.12%.Among them,3.10% was in 2001,1.62% in 2002,1.09% in 2003,0.32% in 2004 and 0.05% was in 2005.And the most was respiratory tract infection,in which the key pathogen causing the infection was Staphylococcus epidermidis.CONCLUSIONS In order to decline the sick rate of hospital infection of newborn baby living in a mother with own baby room,room conditions should be carried out by air disinfection,using disinfected nursing articles and simultaneously doing well the respiratory tract nursing and basic nursing.

OBJECTIVE To transfer the dispersing management non-disposable medical instrument packages into the centralized management by the supply department,to improve its quality and save the medical cost.METHODS The centralized management adopted the step by step implementation strategy,standardized recycling,cleaning,and packaging procedure for key instruments.The cleanliness and integrity of the packaging sheet,the in-packet indicator cards and the out-packet label,and instrument and their human and material resources consumption were compared each other.RESULTS Under the centralized management,the cleanliness and integrity of packaging sheet,the wholeness indicator cards in packet and outside packet of instrument label were obviously better than dispersing management(P

Objective:To observe autophagy in human peripheral blood T lymphocyte.Methods:Peripheral blood T lymphocytes of healthy adults were separated with Percoll(1.073 g/ml) and harvested by using nylon column. The cultured cells were divided into control and dexamethasone(DXM) group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope at 0 and 72 h. And incidence rate of autophagy was analyzed by flow cytometry.Results:①The natural cultured T lymphocytes showed typical morphology of autophagy. ②There was significant statistic difference between 0 and 72 h incidence rate of autophagic T lymphocyte in both control and DXM group. ③There was significant statistic difference in 72 h incidence rate of autophagic T lymphocyte between control and DXM group.Conclusion:Autophagy can be seen in human peripheral blood T lymphocytes, and DXM could induce autophagy.

Objective:To study the morphology and function of the dendritic cells(DC) from human peripheral blood monocytes and the change in cell differentiation and maturation after DC treated with dexamethasone(Dex-DC).Methods:DCs were sorted from human monocytes by culturing in presence of cytokines(GM-CSF, IL-4) and LPS for 8 days with or without dexamethasone. The change in morphology, function and phenotypic characterization was compared.Results:The cells treated with or without Dex, developed a characteristic dendritic morphology, however, Dex-DC expressed higher level of CD14 and lower level of CD83 than the untreated cells. In addition, a low APC function was demonstrated by Dex-DC.Conclusion:DCs can be induced from peripheral blood monocytes in medium with cytokines; the cultured PS in presence of Dex are shown at a more immature stage, indicating that Dex modulates DC differentiation, maturation, and function.

Objective To study the clinical value of preoperative superselective embolization of nasopharyngeal angiofibroma.Methods 14 patients with nasopharyngeal angiofibroma diagnosed by CT and MRI,and proved surgically and pathologically were included in this study.Angiography of bilateral external carotid artery and internal carotid artery as well as vertebral artery was performed in all cases. The external carotid arterial blood supply of tumors were superselectively embolized with 100～300 ?m particles of gelfoam or polyvinyl alcohol(PVA). Surgical resection was performed post-embolization 1 to 3 days. Results The main feeding vessels of tumors were the internal maxillary artery in 9 patients,the internal maxillary artery and ascending pharyngeal artery in 4 patients,the internal maxillary artery,ascending pharyngeal artery and the branches of internal carotid artery in one patient. Preoperative endovascular embolization of the branches of external carotid artery with microcatheter was achieved in all patients. After embolization,tumor stains disappeared completely in 8 patients and partially in 6 patients. The rate of total resection was 92.8%(13/14).The mean amount of haemorrhage during operation was 420 ml and the mean operation duration was 150 min.No major complications were relevant to embolization occurred.Conclusion Preoperative intravascular embolization of nasopharyngeal angiofibroma with microcatheter is a safe and effective method.

Objective:To observe oncosis in human peripheral blood T lymphocytes.Methods:Peripheral blood T lymphocytes of healthy adult was separated with Percoll(1.073 g/ml)and harvested by using nylon column.The cultured cells were divided into control and dexamethasone(DXM)group,and cell morphology was observed through light microscope,electron microscope and fluorescent microscope.And incidence rate of oncosis was analyzed by flow cytometry.Results:①Oncosis could be observed in cultured T lymphocyte after 96h.②In different concentrations of DXM group(1?10-6,1?10-5,1?10-4,1?10-3mol/L),The incidence of oncosis T lymphocytes was(3.49?0.42)%,(5.17?0.48)%,(8.44?0.72)%,(17.93?1.50)%.③During different cultured period(48,72,96,120h),The oncositic rate of T lymphocytes in DXM group was(0.53?0.10)%,(6.36?0.80)%,(20.60?1.59)%,(25.56?1.76)%.Conclusion:Oncosis can be seen in human peripheral blood T lymphocytes,and DXM could induce oncosis.

Objective: To investigate the inspection mode of ethical issues for multicenter clinical trails in China, and to make analysis and give suggestions while taking into consideration of the international conventions. Methods: Representatives from 21 general hospitals and specialized hospitals from Liaoning province, Jiangsu province, Sichuan province, Guangdong province and Shanghai participated in a training course on promotion of ethical review capability; they were surveyed by anonymous questionnaire. Another 20 clinical trial agencies and ethical committees were interviewed by telephone. The questionnaires were collected and valid questionnaires were subjected to statistical analysis. Results: We found that 65.1% of the interviewees never used a central institutional review board (IRB); the main reasons included that they could not assess the quality of central IRB, they wanted to protect the subjects, they had a poor communication with the IRB, and they could not obtain a satisfactorily-localized informed consent forms. There were 34.9% of the interviewees used a central IRB, because they wanted to shorten the inspection time, avoid influence to the research progress, and to have satisfactory quality of IRB. Conclusion: Central IRB is not widely accepted. The related system needs to be further completed so as to improve the outcome of the inspection. The communication and cooperation between the central and local IRBs should be strengthened.

Thirty cases of obesity were treated by acupuncture plus auricular point-embedding method based upon pattern identification, and another 30 cases were treated by single auricular point-embedding method for control study. After the treatments for 30 days, the effective rate was respectively 90.0% and 66.7%, with the statistic significance (P＜0. 05) between the therapeutic effects of the two groups.

Objective:To analyze the clinical therapeutic effect and safety of large dosage of valsartan on chronic heart failure (CHF).Methods:A total of 106 CHF patients hospitalized in our department were chosen and ran-domly divided into routine dose group (n=53,received routine dosage of valsartan,80mg,once/d)and large dose group (n=53,received large dosage of valsartan,80mg,twice/d)accerding to number table.Both groups received anti-heart failure treatment for six months according to the guidelines.After treatment,plasma N terminal pro brain natriuretic peptide (NT-proBNP)level was measured,left ventricular end-systolic diameter (LVESd),left ventricu-lar end-diastolic diameter (LVEDd),interventricular septal thickness (IVST)and left ventricular posterior wall thickness (LVPWT)were measured by color Doppler echocardiography,and all patients received 6min walking test. Therapeutic effect and incidence rates of adverse reactions were compared and analyzed between two groups after treatment.Results: Compared with routine dose group,there were significant reductions in NT-proBNP level [(3042.6±116.3)pmol/L vs.(2565.8±98.2)pmol/L],LVESd [(34.5±2.2)mm vs.(29.4± 2.0)mm], LVEDd [(55.1±2.9)mm vs.(50.2±2.5)mm],IVST [(12.9±1.8)mm vs.(10.7±1.2)mm]and LVPWT [(11.8±1.1)mm vs.(10.9±0.9)mm];significant rise in 6min walking distance [(271.2±24.9)m vs.(367.7 ±22.3)m]and total effective rate (43.40% vs.62.26%)in large dose group,P <0.05 all.There was no signifi-cant difference in incidence rates of major adverse reactions between two groups (P >0.05).Conclusion:Large dos-age of valsartan is more effective and is safe in treatment of chronic heart failure.

Objective To explore the clinical significance of C-reaction protein ( CRP ) and D-Dimer levels in patients with acute exacerbation of chronic obstructive pulmonary disease ( AECOPD ) complicated by pulmonary hypertension (PH) and their association with HP. Methods Arterial blood gas and levels of CRP and D-Dimer were detected in 150 patients with AECOPD. Results Levels of CRP, D-Dimer, and PaCO2 were higher but PO2 level was lower in patients with moderate to severe PH than those with mild PH and the control subjects. Levels of CRP and D-Dimer were higher in patients with mild PH than the control subjects. Levels of CRP, D-Dimer, and PCO2 levels had a linear relationship with PASP, while PO2 was negatively correlated with PASP. Levels of CRP and D-Dimer were positively related with PCO2, while were negatively correlated with PO2. Conclusions Levels of CRP and D-Dimer can be used as an indicator for estimating the severity of pulmonary hypertension in patients with AECOPD.