Objective To observe the clinical effect and the incidence of neuralgia of acupoint injection with nerve growth factor onRamsay Hunt syndrome. Methods 65 patients with Ramsay Hunt syndrome were divided into treatment group (n=33) and control group(n=32). All the patients were treated with the anti-virus and glucocorticoid medicine, and were acupunctured at the similar points with electroacupuncturewith the disperse dense wave. The treatment group was injected with mouse nerve growth factor at the acupoints. 2 days offevery 5 times, 10 times as a course for 2 courses. The treatment effect and the incidence of sequelae neuralgia after 3 months were compared.Results The total effective rate of treatment group (93.9%) was significantly higher than control group (71.9%) (P<0.01). The incidenceof sequelae neuralgia of the treatment group (6.3%) was significantly lower than the control group (26.7%) (P<0.01). ConclusionAcupoint injection of mouse nerve growth factor can improve the efficacy and reduce the incidence rate of sequelae neuralgia in patientswith Ramsay Hunt syndrome.

Objective To study the application of waveforms in treatment of peripheral facial paralysis with electroacupuncture. Methods 66 patients with peripheral facial paralysis were divided into two groups: treatment group (34 cases) and control group(32 cases). All the patients were acupunctured at the similar points with electroacupuncture, while the disperse-dense wave and the discontinuous wave were used successively in the treatment group, and the disperse-dense wave was used alone in the control group. Results There were significant differences between the two groups in the total effective rate and the days for cure (P<0.01). Conclusion The effect of alternate disperse-dense wave and discontinuous wave is superior to that of disperse-dense wave alone in electroacupuncture treatment for peripheral facial paralysis.

Objective To investigate the correlations of serum markers of hepatitis B virus,HBV DNA load and liver function indexes[alanine aminotransferase(ALT)and aspartate transaminase(AST)]in the peripheral blood.Methods Clinical data of 483 patients who were diagnosed with chronic hepatitis B and treated between March 2014 and March 2016 in Tongji hospital were retrospectively analyzed.The serum markers of hepatitis B virus were quantitatively detected by chemiluminescent microparticle immunoassay.Serum HBV DNA load was measured by real-time fluorescence quantitative PCR,and ALT and AST by continuous ultraviolet monitoring.Results There was no correlation between the HBsAg content and HBV DNA load or the rates of abnormal ALT(>41 U/L)and abnormal AST(>35 U/L)(P>0.05).The HBeAg content was not correlated with HBV DNA load and the rates of abnormal ALT(P>0.05),but weakly with the rate of abnormal AST(r=0.21,P<0.01).Although the anti-HBe content was not correlated with the rate of abnormal ALT(P>0.05),it was weakly related to HBV DNA load and the rates of abnormal AST(r=0.16,P<0.05;r=0.19,P<0.01).The anti-HBc content had weak correlations with HBV DNA load,the rates of abnormal ALT and abnormal AST(r=0.25,P<0.01;r=0.29,P<0.01;r=0.29,P<0.01).The logarithm value of HBV DNA load was weakly positively correlated with ALT and AST(r=0.24;r=0.29).Conclusion Quantitative detection of both serum markers and the DNA of hepatitis B virus can complement each other,and when combined with detection of liver function indexes,it will help understand the damage of liver tissue.

Objective To explore the size changes of erythrocytes infected with Plasmodium malariae,so as to improve the basic?level experimenters’microscopy capabilities for P. malariae identification in thin blood smears. Methods The micro?scopic features of erythrocytes infected with P. malariae in thin peripheral blood smears were observed,and a microscope image processing software was used to measure and analyze the diameter changes of the erythrocytes infected with P. malariae. Re?sults The diameter of erythrocytes infected with P. malariae decreased significantly compared with that of the normal erythro?cytes. The three parameters in this study:the diameter of erythrocytes,the value of diameter variation,and the ratio of diameter variation varied at different developmental stages of P. malariae,and the differences were statistically significant(all P<0.01). The variances of the three parameters grouped by different cases or different developmental stages in different cases were ana?lyzed,all showing statistically significant differences(all P<0.01). Conclusion All the developmental stages of P. malariae will cause the decrease of the diameters of infected erythrocytes in peripheral blood smears,but the influence on the diameter of erythrocytes,value of diameter variation,and ratio of diameter variation varies at different developmental stages in different cases.

Objective To study the influence on xerostomia of radiotherapy in treatment of head and neck cancer.Methods 82 cases with head and neck cancer from August 2005 to January 2006 were analyzed retrospectively.All patients were asked about their xerostomia at the end of every week.Then xerostomia and xerostomia-related problems were analyzed.Results There were obvious difference in the incidence rate of xerostomia( ?2=26.24,P 0.05).Conclusion During radiotherapy,acute radiation xerostomia may happen.The rate of it is different.depending on the radiation dose to be accepted while not on the age.

Myoblast transplantation has been carried out for a certain period,but its efficiency in repairing inherited myopathy or severe muscular injury is not satisfactory.The improvements in myoblast culture,transplantation approach,recipient and cell preparation before transplantation,scaffold material and its selection,and body immunity can positively influence transplantation efficacy,and improve recipient function.In addition,these improvements influence the prospect of myoblast transplantation or cell-mediated gene therapy.

OBJECTIVE:To explore the treatment effect and complication prevention of various stent materials for intracranial arteriostenosis.METHODS:A computer-based online search of Science Direct and Ei database (1998-01/2009-10) was performed for English articles with the key words of "intracranial arteriostenosis,stent placement" In addition,CNKI and CBM database (1998-01/2009-10) were searched for related Chinese articles with the same key words in Chinese.Moreover,related works were manually searched.Studies regarding various stent materials for intracranial arteriostenosis were included,including basic and clinical experiments.RESULTS:Multicenter studies of worldwide show that stent implantation displays obvious advantages in treating carotid artery stenosis compared with carotid endarterectomy.Recently,with developing of balloon and stent compliance,as well as stent technology,and application of various novel materials and cerebral protection device,stent has become a safe and effective therapy for intracranial arteriostenosis.However,complications following stent implantation,such as restenosis,ischemic stroke,cerebral hemorrhage hypertransfusion syndrome,and cerebral angiospasm,limit its application.CONCLUSION:The safety and efficacy of intracranial stent implantation remains validation of multicenter and perspective studies.High incidence of restenosis following stent implantation requires further improvement.

Polyamidoamine (PAMAM) dendrimers is considered a better genetic carrier for its ability to form with DNA a nano-sized polyelectrolyte complex of high stability and dissolubility. The mechanism of PAMAMmediated gene endocytosis has been fairly clear and the researches in vitro have confirmed that PAMAM had high transfection efficiency and low cytotoxicity. The researches in vivo have further confirmed that PAMAM had a potential application foreground in the field of gene therapy including in the prevention and treatment of ewing's sarcoma and pulmonary fibrosis, in heart transplantation, and in the preparation of falciparum malaria vaccine.

Objective To observe the effects of esophageal stricture treated with esophageal stenting.Methods 23 esophagus stenosis patients were expanded to 1 lmm,and the stent was implanted-.Results 23 patients were set into successfully,7 cases could enter the common food,11 caces could enter the soft food,5 cases could enter the semiliquid food.18 cases had the ache,6 cases had the stomach esophagus regurgitation symptom.During 1?12 month follow-up,10 cases survived,13 cases died,the cause of death was mainly later period cancer failure and metastasis.Conclusion Esophagus stenosis treatment operation was simple,safe,and the wound small,the short-term curative effect would be good.

Objective To explore the application of mesangial thyroid resection in papillary thyroid carcinoma(PTC) with central lymph node dissection,and its influence on postoperative recurrence.Methods 76 cases with PTC were divided into two groups according to random number table,each group in 38cases.The control group was given primary lesions radical prostatectomy and routine central lymph node dissection,the treatment group was given primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection.The parathyroid function,injury of recurrent laryngeal nerve and local recurrence were compared between the two groups.Results Compared with before surgery,the levels of serum PTH and blood calcium were significantly decreased at the 1st,3rd day after surgery (all P ＜ 0.05),while there were no statistically significant differences in serum PTH and blood calcium at the 7th day after surgery(all P ＞0.05).The levels of serum PTH and blood calcium at the lst,3rd,7th day after surgery between the two groups had no statistically significant differences(all P ＜ 0.05).The incidence rate of injury of recurrent laryngeal nerve in the treatment group was significantly lower than that in the control group (5.3％ vs.21.1％,x2 =4.15,P ＜ 0.05).The secondary surgery rate and local recurrence within 2 years in the treatment group were significantly lower than those in the control group (0％ vs.15.8％,2.6％ vs.18.4％,x2 =6.51,4.69,all P ＜ 0.05).Conclusion Primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection can be used as theroutine operation for PTC,which can effectively produce complete dissection,reduce injury of recurrent laryngeal nerve and local recurrence.