Objective To study the relationship between peripheral blood regulatory T cells CD4 + and the antiviral effect in the patients with chronic hepatitis C.Methods 128 patients with chronic hepatitis C after antiviral treatment and follow-up were col-lected as the experimental group,the peripheral blood was respectively extracted before treatment and after treatment and at 24-week follow-up.The flow cytometry was adopted to detect CD4 + percentage.Moreover the liver function indexes and HCV-RNA quantitation were detected.80 individuals were selected as the normal control group.Results HCV-RNA quantitation,AST,ALT, CD4 + (%)had statistical differences between the the experimental group and the control group(P <0.05);the HCV-RNA quantita-tion,AST,ALT,CD4 + (%)levels after treatment and 24-week follow-up in the experiment group were significantly decreased(P <0.05).Conclusion The antiviral treatment can effectively reduce the damage of chronic hepatitis C,raise the cure rate,reduce the liver damage.The peripheral blood CD4 + regulatory T cells can be used as the index of the antiviral treatment efficacy and hepatitis C virus(HCV)infection.

In the present study we measured the lipid peroxide content in plasma and lung homogenate by Yagi's method, and analyzed the composition of surfactant phospholipid and surface activity of pulmonary surfactant by thin layer chrom-otography and by Wilhelmy's film balance.The results showed that the lipid peroxide level was increased in plasma and lung homogenate; licithine,tysopho-spholipidcholine and phosphatidylethanolamine were decreased in bronchial-alveolar lavege fluid; and pulmonary surfactant surface activity was lowered.The findings suggest that decreased surface activity may result from the decrease of disaturated phospholipidcholine, which may be due to its impaired synthesis.

Objective To study the retrieved human allografts pathologically in order to probe into the histological changes of implanted allograft. Methods The specimens were retrieved from seven cases between February 2003 and March 2004, and the allografts had been in situ from two to 16 months. Of seven cases, four were diagnosed primarily as malignant fibrohistocytoma of proximal humerus, osteosarcoma of femoral shaft, bone giant cell tumor of distal femur and bone giant cell tumor of proximal tibia each, which were treated with wide resection of tumor and massive allograft transplantation and internal fixation of plate or inter-locking nail; two were open comminuted fracture of distal femur, treated with massive allograft transplantation and internal fixation, underwent autograft bone transplantation because of united massive allograft, had a punch biopsy of allografts for observation; one was tibial open fracture being fixated with inter-locking nail and small segment allograft transplantation, sustained infection at two weeks after primary operation and underwent debriment, removal of internal fixation and external fixation. Results Of seven cases, five were found with bone absorption radiographically, and were classified into three types:1)sever bone absorption; 2)bone absorption associated with sinus and effusion of the wound; 3)bone absorption accompanied by pyogenic infection. The later condition was caused by bacterial infection without a relationship of allograft. And the former two conditions could be classified into two subtypes: 1)osteoclastic absorption, which was followed by new bone formation; 2)inflammatory absorption, which was found with infiltration by round inflammatory cell, preponderantly lymphocytes, and mononuclear phagocytes with few osteoclasts, osteoblasts or new bone formations. Moreover, in sever bone absorption, vascular lesions such as vasculitis, swelling of endothelial cells or proliferation changes in the vessels, and obliteration of the arteries. Conclusion An intensive infiltration accompanied by vascular lesions may be the evidences of histological changes suggestive of an immune reaction directed against the grafts, and the failure of the clinical outcomes.

Objective To study the expressions and clinical significance of matrix metalloproteinases-2(MMP-2) and vascular endothelial growth factor-C(VEGF-C) in patients with papillary thyroid carcinoma(PTC).Methods SP immunohistochemical technique was used to detect the expressions of MMP-2 and VEGF-C in 78 cases of PTC and 18 cases of thyroid benign tumors.Results The positive expression rates of MMP-2 and VEGF-C in PTC(80.77%,75.64%) were significantly higher than those of the thyroid benign tumor(11.11%,22.22%),P

Objective To study the relationship between liver histological changes and expression of HBcAg in peripheral blood dendritic cells in patients with chronic hepatitis B.Methods The peripheral blood samples were obtained from 24 patients with chronic hepatitis B (CHB) and 8 healthy individuals.The peripheral blood mononuclear cells (PBMCs) were isolated.DCs were isolated from peripheral blood mononuclear cells and enriched by culturing with recombinant human interleukin 4(rhIL-4) and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF),then DCs were harvested and the costimulators of CD80,CD86,CD40 and CD54 on DCs were tested by immunofluorescence flow cytometry.These costimulators were compared with liver histological changes.Results Percentage of CD40 and CD86 was significant lower than that of healthy donors(P

0 05,both).The mean level of COX 2 mRNA expression in active SLE group was significantly higher than that in normal controls ( P 0 05).Conclusion The level of COX 2 mRNA expression in active SLE group is markedly increased,which may be involved in the pathogenesis of SLE and provides a valuable scientific basis on the clinical use of specific COX 2 inhibitors.

The management for qualification documents of medical instruments is very important work to management department of medical instruments. Because the number of qualification documents of medical instruments is very large and they have an expiry date, it is difficult to manage them. This article discussed how to manage qualification documents of medical instruments, and an information management system that has a function of traceability management has been developed. This information management system standardizes management for qualification documents of medical instruments, and ensures that qualification documents of medical instruments are available and can be traced. Besides, it can reduce the amount of work for medical instruments management.
Documentation ; standards ; Equipment and Supplies ; standards ; Information Systems

Objective:To observe the sensitivities of breast cancer cells and its implanted tumors to chemotherapeutic drug epirubicin after down-regulation of HER-2 expression by RNA interference(RNAi).Methods:HER-2siRNApU6 vector containing HER-2 RNAi was constructed and was used to transfect HER-2 positive breast cancer cell line SKBR-3.The expression of HER-2 mRNA and protein were analyzed by RT-PCR and Western blotting,respectively.Transfected SKBR-3 cells were treated with different concentrations of epirubicin;the growth of SKBR-3 cells and IC50 of epirubicin were observed by MTT.SKBR-3 cells were injected into nude mouse to establish breast cancer model;the sensitivity of mouse model to epirubicin was observed after HER-2shRNApU6 treatment.Results:Expression of HER-2 mRNA and HER-2 protein were down-regulated in SKBR-3 cells after transfection with HER-2shRNApU6.Furthermore,the proliferation of SKBR-3 cells transfected with HER-2shRNApU6 was significantly decreased compared with mock tansfected group(P

Objectives To explore a comprehensive approach for the functional and aesthetic correction of septal deviation. Methods From Jan. 2007 to Dec. 2009, 48 patients with deviation of the frontier and ceiling border of the septal cartilage, 6 males, 42 females, average age of 26.5 years, were surgically treated with a comprehensive use of several techniques, such as septoplasty, reconstruction of septal support, precise osteotomies and nasal tip plasty. Results Functional improvement achieved in all the patients, contour satisfaction achieved in 91.7 % patients; 30 patients were very satisfied, and 14 satisfied with the outcome. Conclusions With the thorough understanding of surgical anatomy of the nose and the use of comprehensive surgical approach, satisfactory outcomes can be achieved both functionally and aesthetically in patients with septal deviation.

Objective To compare the short-term safety and efficacy of radiofrequency ablation (RFA) and microwave ablation (MWA) for treatment of large benign thyroid nodules.Methods A total of 34 patients with large benign thyroid nodules were studied retrospectively,including 13 patients treated with ultrasound-guided RFA and 21 patients treated with MWA between Jun.,2016 and Feb.,2017 in Zhongshan Hospital.The thyroid function parameters,serum antibodies,complications and thyroid nodules volume reduction rate(VRR) were compared between the two groups during the follow-up.Results There were no statistically significant differences(P＞0.05)among those patients before and after treatment in serum free triiodothyronine (fT3),free thyroxine (fT4),thyroid stimulating hormone (TSH),anti-thyroglobulin antibodies (TG-Ab) levels,and thyroid peroxidase antibodies (TPO-Ab).One patient in the MWA group had mild hoarseness after ablation and another patient in the RFA group had intraoperative hemorrhage for about 10 mL.There were no other complications such as neck scar,postoperative infection,skin burns,tracheal and esophageal injury.One day after the ablation,all nodules were showed hypoecho and contrast-enhanced ultrasound proved there was no blood supply.One month after treatment,no statistically significant difference was found in VRR between two groups (23.8％ vs.22.6％,P =0.127).Conclusions RFA and MWA are safe and effective treatments for large benign thyroid nodules,and no significant difference was observed in short-term follow-up.