Objective: To investigate relationship between sexual behavior and health-risk behavior of college students, and to provide suggestions for sex education in university. Methods: Using stratified cluster sampling and selected 1 585 college students to complete questionnaires in Chongqing University of Arts and Sciences. Results: The sexual behavior rate was 16.1%, energy drinking rate was 39.2%, smoking rate was 18.0%, alcohol drinking rate was 34.8%, gambling rate was 9.2%. There were significant differences between different gender, native place, drinking energy drink, smoking, drinking alcohol, gambling students’ sexual behaviors’ rates(χ2=42.84, 31.11, 40.12, 18.96, 53.66, 70.17, 42.50,P<0.01).The results of multivariate logistic regression showed that, drinking energy drink(OR=0.71, 95%CI=0.53-0.96), somking(OR=0.47, 95%CI=0.33-0.66), drinking alcohol(OR=0.50, 95%CI=0.37-0.69), gambling (OR=0.52, 95%CI=0.35-0.79)was positively associated with sexual behavior of college students(P<0.05). Conclusion The sexual behavior co-occurs with energy drinking, smoking, drinking and gambling; the college should lead students to keep away from health-risk behaviors in sexual education.