Objective: To investigate relationship between online partner seeking sexual behavior and sensation seeking risk behaviors among college students, and to provide suggestions on college sex education. Methods: Using convenience sampling, a self administered questionnair survey was administered among 1 923 students in one college in Chongqing between September and October in 2021, regarding online partner seeking sexual behavior,sensation seeking and risk behaviors. Results: About 26.7% of participants reported having sexual behavior, and 12.3% students having online partner seeking sexual behavior. There were no significant sex differences in online partner seeking sexual behavior ( χ 2=1.25, P >0.05); partner seeking sexual behavior varied by grade, gambling,alcohol drinking and internet addiction tendency( χ 2=9.40, 3.29, 19.63 and 3.66, P <0.05), which was higer among college students in grade one(9.1%), no gambling habit(12.0%), drinking habit(8.4%), and having no inclination to Internet addiction (11.8%) compared with those of grade 2 and 3(14.4%, 13.1%), gambling habit(19.7%), no drinking habit( 15.1 %) and having inclination to Internet addiction (16.6%). The results of multivariate Logistic regression showed that alcohol drinking ( OR =0.45，95% CI =0.33-0.62) was negatively associated with online partner seeking sexual behavior, sensation seeking ( OR =1.08，95% CI =1.03-1.13) and gambling ( OR =2.15，95% CI =1.19-3.88) were positively associated with online partner seeking sexual behavior of college students( P <0.05). Conclusion Colleges should pay attention to the occurrence of online partner seeking sexual behavior, provide guidance for recognition of adverse consequences of sensation seeking behavior, and to avoid the clustering of online partner seeking sexual behavior with gambling behaviors.