Objective: To investigate relationship between campus violence and sexual orientation of middle school students, and to provide suggestions for intervention of campus violence. Methods: A total of 3 738 middle school students selected by using stratified cluster sampling method were asked to attend a questionnaire survey. Results: About 49.9% middle school students were campus violence victims. There were significant differences between different school, gender, education level of parents, academic achievement, sexual orientation students’ rate of suffering from campus violence(χ2=18.66, 88.35, 7.71, 8.26, 10.07, 15.23, P<0.01), junior students’(53.4%) was higher than senior students’(46.3%), boys’(58.1%) were higher than girls’(42.7%), students’ whose parents’ education level greater than equal or undergraduate degree(54.0%, 54.6%) was higher than those whose parents’ education level below undergraduate degree(48.6%, 48.7%), students whose study achievement below average (54.5%) was higher than those whose achievement greater than or equal to average(48.9%, 47.9%), homosexual and bisexuals’(both 58.7%) were higher than those with uncertain sexual orientation and heterosexuality(51.6%, 48.1%). Homosexual (OR=1.51) and bisexual (OR=1.68) were risk factors for suffering from campus violence of middle school students. Conclusion Middle schools should strengthen the prevention and intervention of campus violence; Students should be provided with proper information regarding homosexual and bisexual, and to reduce and avoid violence against them.