A disintegrin and metalloproteinase 10 is a zinc dependent transmembrane type Ⅰ multi-domain of protein which is highly conserved in evolution and mediates protein maturation and ectodomain shedding, with protein hydrolysis, cellular adhesion, cellular fusion and signal transmission.This article reviews its structure and function, the substrate and relative signaling pathways, the factors influencing its expression and catalytic activity,the research progress in urinary tract diseases.

Acute kidney injury (AKI) represents a common and devastating problem in clinical medicine. The lack of early biomarkers for AKI has led to a delay in initiating potentially effective therapies.Identification of novel biomarkers for AKI has progressed to the clinical phase of the biomarker discovery process. The most promising AKI biomarkers include neutrophil gelatinase-associated lipocalin, IL-18, kidney injury molecule-1 and liver-type fatty acid binding protein. In this article, we review the study of neutrophil gelatinase-associated lipocalin,IL-18, kidney injury molecule-1 and liver-type fatty acid binding protein in recent years,in order to provide the theory basis for the clinical application of the early biomarkers for AKI.

AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis. Recent studies demonstrated that AMPK is a novel signaling molecule modulating inflammatory responses and oxidative stress which are involved in inflammatory pulmonary diseases, such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary infectious diseases and pulmonary fibrosis. AMPK attenuates inflammatory lung injury by phosphorylating its downstream targets, such as sirtuin1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha), p53 and forkhead box O3a (FoxO3a). This review summarized the relationship between AMPK and the development of inflammatory pulmonary diseases.
AMP-Activated Protein Kinases ; metabolism ; Forkhead Box Protein O3 ; Forkhead Transcription Factors ; metabolism ; Homeostasis ; Humans ; Inflammation ; enzymology ; Lung Diseases ; enzymology ; Oxidative Stress ; PPAR gamma ; metabolism ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ; Phosphorylation ; Sirtuin 1 ; metabolism ; Transcription Factors ; metabolism ; Tumor Suppressor Protein p53 ; metabolism

Objective To study the effect of live combined bacillus subtilis and enterococcus faecium granules with multivitamines (Medilac-Vita) and nonnutritive suck on the premature infant.Methods Eighty premature infants without asphyxia and digestive tract malformation were randomly divided into control group(without Medilac-Vita and nonnutritive suck group) and observation group( Medilac-Vita and nonnutritive suck group) including 40 cases each.The gastrointestinal dysfunction and growth-development index were observed in two groups.Results In observation group,the occurrence rate of vomit,abdominal distention,gastric retention,hemorrhage of digestive tract,meconium peritonitis and necrotizing enterocolitis were all lower than that in control group.The cleaning lime of meeoninm in observation group was lower than that in control group [(3.20±1.01 )d and (6.23±2.56)d],the removing time of canalis ventriculi [(4.03±2.11 )d and (7.10±2.91 )d],the time of the jaundice fading away [( 11.10±5.36)d and ( 14.30±6.82)d ],the time of staying in hospital [(15.02±3.12 )d and (20.00 + 5.11)d],the growth of the head circumference and weight in seven and fourteen days were significantly different between two groups.The incidence rates of intracranial hemorrhage,apnea and death in observation group were 12.5% (5/40),10.0% (4/40) and 0 (0/40),which in control group were 45.0%(18/40),37.5% (15/40) and 5.0% (2/40).Conclusion The treatment of Medilac-Vita and nonnutritive suck on the premature infants can promote the maturity of stomach and intestine function,reduce the occurrence of complications,promote growth and development.

Objective To investigate the epidemiologic features of infant rotavirus (RV) diarrhea in Nanjing area .Methods The detection of group A rotavirus were performed on 7 325 outpatients from intestinal clinic of Nanjing Children′s Hospital by using immune colloidal gold method ,and the epidemiological analysis were did according to the detection results .Results In the 7 325 pa-tients ,2 353 were found rotavirus positive ,the total positive rate was 32 .12% .The positive rate of 1 -2 years old group was the highest(43 .51% ) ,compared with other age groups (P< 0 .05) .In November ,December and January ,the positive rates were 52 .20% ,46 .98% ,46 .59% ,respectively .Compared with the positive rate in other months ,there was statistically significant differ-ence(P<0 .05) .The infection rate of RV in vaccine group and non-vaccine group were 9 .06% and 38 .74% ,respectively .There was significant difference between the two groups .Conclusion Infants of 6 months to less than 3 years old in Nanjing have higher de-tection rate of RV infection ,and the infection peak appears in autumn and winter .

Objective Using PBL and CBS pedagogy,to explore the feasibility of the application and effectiveness of teaching in medical genetics teaching. Methods In medical genetics teaching,set the clinical Class 1 grade 2007 to the control group,using traditional pedagogy,set the clinical Class 2 grade 2007 to the experimental group,using PBL and CBS pedagogy. Results Final exam results showed that the experimental group's average scores were significantly higher than control group(P

Cell polarity is a common feature of many different types of cells,and it is essential to the normal differentiation and function of cells. Partitioning defective 6( PAR6)gene encodes PAR6 protein, which is crucial to asymmetric cell division and polarized growth. PAR6 protein as a member of the PAR6 polarity complex,affects the synthetic of centrosome and protein recruitment to the centrosome. The abnormal number of centrosomal and the loss of cell polarity may eventually lead to the occurrence of tumor.

The training of clinical thinking ability is the key point of obstetrical teaching to medical students in eight-year program.Obstetrical department of the third affiliated hospital of Sun Yat-sen university has put forward specific methods of SP inquiring and PBL case analysis teaching.The well-designed teaching methods have an active role in the training of case history collecting ability,diagnosing and treating ability to medical students in eight-year program.

0.005.The management center of Chinese Hematopoietic Stem Cell Donor Database checked the information through the network,and there had been 20 cases of successful transplantation using stem cells donated by Heilongjiang donors by March 2007.ConclusionDistribution of HLA-A,B,DRB1 alleles and haplotypes in Heilongjiang Chinese Han is close to that in Northern Chinese Han population,similar to that of the whole Chinese Han population.

Objective To observe the changes of neutrophil gelatinase associated lipocalin,NGAL after endoplasmic reticulum stress by thapsigargin in renal tubular epithelial cells.Methods The renal tubular epithelial cells were treated with thapsigargin to establish the model of endoplasmic reticulum stress (ER group).CHOP and GRP78 expression were detected by Western-blot to confirm endoplasmic reticulum stress in renal tubular epithelial cells by thapsigargin.NGAL expression were detected by Western-blot in ER group and control group(normal renal tubular epithelial cells).Results After treated with 2.5 μmol/L and 5 μmol/L thapsigargin for 4 hours and 8 hours respectively,endoplasmic reticulum stress in the renal tubular epithelial cells were significantly increased comparing with control group (0.585 ± 0.045 to 0.523 ± 0.030;0.785 ± 0.049 to 0.728 ± 0.064),which was approved by the increased expression of CHOP and GRP78 (P ＜ 0.05).The expression of NGAL was significantly increased in ER group (0.567 ± 0.024 to 0.826 ± 0.057,P ＜ 0.05) and it was corresponded with the changes of CHOP and GRP78,which increased significantly with the severity of endoplasmic reticulum stress.Conclusion Endoplasmic reticulum stress can be induced by thapsigargin in renal tubular epithelial cells.The expression of CHOP,GRP78 and NGAL were significantly increased correspondingly with the severity of endoplasmic reticulum stress.It is speculated that the expression of NGAL may be related with endoplasmic reticulum stress in tubular epithelial cells.