BACKGROUND: HLA-B27 typing has long been performed by the microlymphocytotoxicity method(MCT) but the flow cytometry method(FCM) was introduced several years ago. False positive results due to the HLA-B7 cross reactive groups(CREG) were the main drawback of the serologic method. The authors performed polymerase chain reaction-sequence specific primer(PCR-SSP) test for HLA-B27 to compare the results with serologic methods. METHODS: PCR-SSP test for HLA-B27 was performed on four hundred forty one samples. Three hundred twenty eight samples were tested by MCT and one hundred thirteen samples by FCM. PCR-SSP for HLA-B27 subtyping or Amplification Refractory Mutation System-PCR(ARMS-PCR) for HLA-B typing was performed on twenty four discrepant samples. RESULTS: The concordance rate between MCT and PCR-SSP was 92.9%(305/328) and the concordance rate between FCM and PCR-SSP was 99.1%(112/113). Twenty four(5.4%) out of four hundred forty one samples showed discrepancy between serologic methods and PCR-SSP method. Fourteen out of one hundred MCT positive samples and only one out of forty FCM positive samples showed negative by PCR-SSP. Nine samples showed PCR-SSP positive and MCT negative. CONCLUSIONS: The false positive rate of MCT was quite high and there were some false positive and negative results by PCR-SSP, too. From the above findings, we suggest that FCM is the most accurate method for HLA-B27 typing in those laboratory equipped with flow cytometry.
Flow Cytometry* ; HLA-B Antigens ; HLA-B27 Antigen* ; HLA-B7 Antigen

OBJECTIVE: HLA-B27 test require at least 80% of viable cells. So the procedures require immediate processing of the blood samples. To increase the efficiency and the cost-effectiveness of the laboratory, longer maintenance of cell viability is requested. For this purpose, we compared the cell viability of blood samples collected in heparin tubes with those collected in ACD tubes. Our aim was to determine whether the length of storage that could maintain adequate cell viability for HLA-B27 testing depends on the anticoagulants in the storage tube and to ascertain the qualified HLA-B27 results after several days of storage. METHODS: We collected 15 blood samples in ACD and heparin tubes. The samples were stored at 4oC for 6 days and after which, 2 mL of the sample was used to confirm the viability of lymphocytes every 24 hours for 5 days. For evaluating the reproducibility, the HLA-B27 test was performed at the day of sampling and after 6 days of refrigeration. RESULTS: There was no difference in cell viability between ACD and heparin until the sixth day, but a statistically difference was observed from the seventh day (p=0.004). The HLA-B27 test showed no different results even after 6 days of storage. CONCLUSION: We suggest that the heparin tubes have a same storage benefit as the ACD tubes of blood for HLA-B27 testing for less than a week. This can affect more economic and efficient laboratory management for HLA-B27 testing.
Anticoagulants* ; Cell Survival* ; Heparin ; HLA-B27 Antigen* ; Lymphocytes ; Refrigeration

No abstract available.
Arthritis, Psoriatic* ; HLA-B27 Antigen ; Korea* ; Prevalence* ; Psoriasis ; Spondylitis

The frequency of HLA-B27 antigen in 24 patients with psoriasis vulgaris and 11 patients with psoriatic arthritis were examined using the standard micro-lympho- cytotoxicity technique. There was a high frequency of HLA-B27 in psoriatic spondylitis with or without peripheral arthritis (83.3%) compared to controls (4.7%) and the relative risk of HLA-B27 for developing psoriatic spondylitis was 82.5% which indicates a strong association between HLA-B27 and psoriatic spondylitis in Korean populations. However, there was no stastical significance observed in the groups of patients with peripheral arthritis alone or psoriasis vulgaris in regard to the frequency of HLA-B27.
Arthritis ; Arthritis, Psoriatic ; HLA-B27 Antigen ; Humans ; Psoriasis* ; Spondylitis

BACKGROUND: The flowcytometric analysis of HLA-B27 gives more objective results and is performed more rapidly than traditional serologic methods. We have used a flowcytometric method using only anti-HLA-B27 monoclonal antibody, but it gave frequently borderline mean fluorescence intensity (MFI) results. The authors compared the method using anti-HLA-B27 antibody (HLA- ABC-m3, Serotec) with the Becton Dickinson (BD) method which uses different HLA-B27 antibody (GS145.2) with CD3 antibody. METHODS: The 59 patients that requested HLA-B27 testing were measured by two methods. In the former method, the mononuclear cells were stained with HLA-B27-FITC and the MFIs were determined in lymphocytes. In the BD method, the whole blood was directly stained with CD3-PE and HLA-B27-FITC. The MFIs were determined in the CD3+ cells, and compared with the MFI of the standard. For the cases showing discrepancy in the two methods or borderline values, the HLA-ABC typing was done. RESULTS: Of 21 showing discrepancy, 10 samples had undergone HLA typing. Among nine samples that were positive by the Serotec method but negative by the BD method, four samples were B7, one B40, one B54 and three B7 CREG negative. One that was negative by the Serotec method but positive by the BD method was confirmed as HLA-B27. CONCLUSIONS: The Serotec method showed significant overlap between the MFIs of HLA-B27 and non B27 samples that resulted in a relatively low efficiency compared with the BD method. The discrepant results of the two methods seem to be due to maily the specificity of the antibody used.
Fluorescence ; Histocompatibility Testing ; HLA-B27 Antigen* ; Humans ; Lymphocytes ; Sensitivity and Specificity

HLA-B Antigens ; genetics ; HLA-B27 Antigen ; genetics ; Humans ; Spondylitis, Ankylosing ; genetics

A 30-year-old female patient with coexisting ankylosing spondylitis and rheumatoid arthritis was diagnosed and treated. The human leukocyte antigen (HLA)-B27 is a predisposing factor of ankylosing spondylitis and HLA-DR4 is a predisposing factor of rheumatoid arthritis. This patient was HLA-B27 and HLA-DR4 positive, and ankylosing spondylitis manifested before rheumatoid arthritis. After disease modifying anti-rheumatic drugs successfully arrested ankylosing spondylitis activity the patient conceived and delivered a healthy baby. One year later, she developed peripheral polyarthritis and was diagnosed with rheumatoid arthritis. We hypothesized that pregnancy may be one of the environmental factors that can activate rheumatoid arthritis, and that disease modifying anti-rheumatic drugs play an important role in keeping the disease under control.
Adult ; Arthritis, Rheumatoid ; diagnosis ; metabolism ; Female ; HLA-B27 Antigen ; metabolism ; HLA-DR4 Antigen ; metabolism ; Humans ; Spondylitis, Ankylosing ; diagnosis ; metabolism

In order to evaluate association of particular HLA typing with certain uveitis in Korean population, HLA antigens were analyzed in 114 uneitis patients(acute anterior uveitis: 32 cases, Behcet`s disease: 25 cases, intermediate uveitis: 19 cases, Vogt-Koyanagi-Harada (V-K-H) syndrome: 10 cases, retinal vasculitis: 12 cases, Eale`s disease: 3 cases, posterior uveitis: 9 cases, pan.uveitis: 4 cases). The stronger association between acute anterior uveitis and HLA-B27 was statistically significant, and this result was similar to reports in other ethnic groups. Also, the association between V-K-H syndrome and HLA-DR4 showed same results. But the high frequency of HLA-DR7 in the patients with V-K-H syndrome was unque in patients of Korean popjlation and statistically significant. The association between HLA-A2 and posterior uveitis was high in patients of Korean population and statistically significant. Behcet`s disease was stronger association with HLA-B51 but not statistically significant and much weaker association than reports in Japanese group. Although many similarities of associations between particular uveitis and HLA typing were detected as compared with other ethnic groups, distinctive HLA associations were demonstrated in Korean population. Additional cases and long-term follow-up are required to confirm the association with HLA typing and the relationship with prognosis including clinical and laboratory variabilities.
Asian Continental Ancestry Group ; Ethnic Groups ; Follow-Up Studies ; Histocompatibility Testing ; HLA Antigens ; HLA-A2 Antigen ; HLA-B27 Antigen ; HLA-B51 Antigen ; HLA-DR4 Antigen ; HLA-DR7 Antigen ; Humans ; Prognosis ; Retinal Vasculitis ; Uveitis* ; Uveitis, Anterior ; Uveitis, Intermediate ; Uveitis, Posterior

About 10% ~ 40% of patient with Marie-Strumpell ankylosing spondylitis have anterior uveitis. Recently, relationship between ankylosing spondylitis and anterior uveitis was discoverd by several studies of HLA-B27 antigen. Ankylosing spondylitis was found to be the commonest single etiological factor in analysis of 653 cases of uveitis seen in the Uveitis Clinic in United Kingdom. Ankylosing spondylitis is mostly found in male and most of their natures of uveitis are acute unilateral nongranulomatous anterior uveal involvement. Roentgenological findings of ankylosing spondylitis are characteristic demineralization and subchondral sclerosis of sacro-iliac joint and "Bamboo spine". Elevation of erythrocyte sedimentation rate in ankylosing spondylitis indicates active status of the disease. Authors have experienced 2 cases of anterior uveitis with Marie-Strumpell ankylosing spondylitis in our clinic. These cases have shown typical findings of "Bamboo spine", sacro iliac joint blurring and acute nongranulomatous unilateral uveal involvement.
Blood Sedimentation ; Great Britain ; HLA-B27 Antigen ; Humans ; Joints ; Male ; Sclerosis ; Spondylitis, Ankylosing* ; Uveitis ; Uveitis, Anterior*

Ankylosing spondylitis (AS) is a chronic autoinflammatory disease that affects the spine and sacroiliac joints. Regarding its etiology, although HLA-B27 is known to be the strongest genetic factor of AS, much evidence suggests the potential contribution of non-MHC genes to the susceptibility to AS. Most of these non-MHC genes have been discovered in non-Asian populations; however, just some of them have been validated in Koreans. In this study, we aimed to identify additional AS-associated single-nucleotide polymorphism (SNP) candidates by replicating the candidate SNPs in Korean AS patients and healthy controls. For this, we selected three SNPs (rs11249215 in RUNX3, rs6556416 in IL12B, and rs8070463 in TBKBP1), which were previously reported as risk factors of AS but have not been studied in Koreans, and performed genotyping assays using a total of 1138 Korean samples (572 AS patients and 566 healthy controls). Of the three SNP candidates, one SNP in RUNX3 (rs11249215) was significantly associated with the risk of AS (odds ratio, 1.31; 95% confidence interval, 1.02 to 1.68, p = 0.03). These results will be helpful in elucidating the pathogenesis of AS and may be useful for developing AS risk prediction models in Koreans.
HLA-B27 Antigen ; Humans ; Polymorphism, Single Nucleotide ; Risk Factors ; Sacroiliac Joint ; Spine ; Spondylitis, Ankylosing*