The paper introduces the background of YY/T 0841 standard, and interprets the main content of this standard. It is helpful to understand and applicate the standard.
Electrical Equipment and Supplies ; standards

Objective To explore the biological function of ICAM-1and LFA-1 in tumor metastasis,we observed the expression of ICAM-1(Intercellular Adhension Molecule-1)and LFA-1(Lymphocyte function related antigen-1)in cancer tissue and normal tissue.Methods A total of the samples were obtained from hospital,it includes 60 cases of breast cancer and 60 cases of normal mammary tissues.We observed the expression of ICAM-1 and LFA-1 by SP immunohistochemistry.We analyzed the relationship between the expression of ICAM-1,LFA-1and lymphatic vessel metastasis of breast cancer.Results The expression positive rate of ICAM-1and LFA-1 in breast cancer was obviously higher than that in normal mammary tissues(P

Objective: To explore therapeutic effect of levosimendan combined dobutamine on refractory heart failure (RHF).Methods: A total of 90 RHF patients, who were treated in our hospital from Apr 2014 to Oct 2015, were selected.According to random number table, patients were randomly and equally divided into dobutamine group and combined treatment group (received dobutamine combined levosimendan).Cardiac function improvement after treatment,and adverse reactions during treatment were compared between two groups.Results: After treatment, cardiac function improvement rate of combined treatment group was significantly higher than that of dobutamine group (86.7% vs.60.0%), P=0.02.Compared with before treatment, after treatment, there was significant reduction in dyspnea score, and significant rise in LVEF and stroke votume(SV) in both groups (P<0.05 or <0.01);compared with dobutamine group after treatment, there was significant reduction in dyspnea score [(1.58±0.78) scores vs.(1.02±0.47) scores], and significant rise in LVEF [(42.61±7.10)% vs.(50.21±7.82)%] and SV [(57.62±14.63)ml vs.(64.21±15.12)ml] in combined treatment group, P<0.05 or <0.01.Hypotension, myocardial ischemia and arrhythmia etc.occurred in both groups, but there was no significant difference in incidence rate of total adverse reactions (P>0.05).Conclusion: Levosimendan combined dobutamine possess significant therapeutic effect on refractory heart failure and safety is good, which is worth extending.

Female ; Hospital Administration ; Humans ; Male ; Physical Examination ; methods

Objective:To analysis the N-terminal amino acid sequence of 5D3Ag purified by mAb5D3 immunoaffinity column.Methods:SDS-PAGE and Western Blot were used to study the characteristics of 5D3Ag.From SDS-PAGE gel,5D3Ag was transferred to PVDF membrane and its N-terminal amino acid sequence was analysised with an auto amino acid sequencer.Results:SDS-PAGE of 5D3Ag showed a band on the gel with molecular weight of 43 kD.Western Blot study showed that mAb5D3 can recognize 5D3Ag.The N-terminal amino acid sequence was NQGSHGSEEQ.Conclusion:It is sure that 5D3Ag is unique to Osteosarcoma because of the purification method itself and the characteristics of mAb5D3.Compared with other reported Osteosarcoma associated antigen,5D3Ag is different from them in molecular weight and may be a novel Osteosarcoma associated antigen.With the help of N-teminal amino acid sequence,some primers may be designed to clone the whole length gene of 5D3Ag.The identification of 5D3Ag gene may be useful in understanding the biologic mechanics of Osteosarcoma and developing new diagnostic and therapeutic strategies.

With the development of chemical analysis technology and data processing technology,metabonomics has become the important method in functional genomics study.In this paper,the definition and advangtages of metabonomics are provided.The implication of metabolomics in pharmacology are summarized and future perspectives are discussed.

Objective To discuss the effect of treating naloxone on acute organphosphours poisoning (AOPP).Methods 64 patients with AOPP were randomly divided into group treated with naloxone(32,group A) and group treated with routine medical therapy(32,group B).Group A was treated with naloxone after routine medi- cal therapy.Results Group A:time of the achievement of atropinization was shorter,doses of atropine was smaller than group B(P

This paper introduces the rapid & mobile first-aid unit from brigade or regiment medical aid station,especially discusses its equipment,performance,personnel structure,and working features.The rapid & mobile first-aid unit can perform emergency operation and anti-shock treatment for the serious wounded in frontier at any moment.It meets the requirements in such aspects as fast & effective first-aid,mobile & flexible treatment,high cure rate and good practicality.

Objective To study the anti-tumor effect of water extracts from Sophora Moorcroftiana seed(SMSWE) in vivo on mice.Methods S180-bearing mice group were observed.The inhibitory rate of tumor,survival period,indexes of thymus and spleen,the proliferative ability of T lymphocytes were assayed.Results 1.2 and 2.4 g/kg SMSWE could inhibit the growth of S180 tumor in mice and prolonged survival period(P

Objective To explore the influence of organizational climate and job burnout on turnover tendency among contract nurses, and establish a model of this influence. Methods Totally 450 nurses were selected and investigated with Organizational Climate Questionnaire(OCQ), Maslach job burnout inventory(MBI) and Turnover Intention Scale(TIS). Results The total score of OCQ was 2.71 ± 0.58, the emotional exhaustion was 25.99 ± 13.89, the depersonalization was 10.21 ± 5.37,the personal accomplishment was 21.92 ± 9.27, and the score of TIS was 2.66 ± 0.94. Emotional exhaustion and depersonalization were significantly and negatively correlated to OCQ(r=-0.26,-0.22 ,P<0.01),and positively correlated to TIS(r=0.27,0.25,P<0.01). The personal accomplishment was positively correlated to OCQ(r=0.26,P<0.01), and negatively correlated to TIS(r=- 0.26,P<0.01).OCQ was negatively correlated to TIS(r=-0.37,P<0.01).Multiple linear regression analysis showed that OCQ and personal accomplishment could negatively predict nurses′ turnover tendency(β=-0.43,-0.14,P<0.01), and emotional exhaustion could negatively predict nurses′ turnover tendency(β=-0.10,P<0.01). The path analysis showed organizational climate negatively influenced nurses′ turnover tendency via job burnout, and the value of mediating effect was 23.8%(-0.10/-0.42). Conclusions Turnover tendency is significantly correlated with organizational climate and job burnout among contract nurses. The relationship between organizational climate and nurses′ turnover tendency among contract nurses is mediated in part by job burnout.