Objective] To study the transmission of the Ishinpo versions ,understand the prevailing version of Ishinpo. [Method] With the research methods of the Chinese traditional history and philology,collect and sort historical cited references of the Ishinpo, by paying attention to three main emendation in the transmission history of Ishinpo. [Result] Ishinpo of Nakarai is the golden version for document research. The prevailing Ansei Ishinpo version of contemporary China is based on Nakarai version,which consists of twenty seven scrolls of Hei era and three scrolls of Edo Era. Ishinpo Versions have been divided into Emperor Nakarai versions,Uji versions and Family tradition versions. There are big differences in utilization and completeness of the three version system. The Emperor Nakarai versions are relatively good, of which the Nakarai version preserves best. The Uji versions are widespread, of which the RenHe Temple version is better preserved but lacuna. There are lots of lacunas in the family tradition versions, owing to circulateed copies. [Conclusion] In the three main emendation, of Ishinpo, mostly, the Nakarai version is the source edition, the Uji version is primary edition, and the family tradition version is the secondary edition. The paper, which is useful in the transmission of the Ishinpo and thus historical process of medical exchange between China and Japan, has established the system of Ishinpo versions, defined the golden version and the prevailing version.

The ghrelin is an endogenous ligand of the growth hormone (GH) secretagogue receptor,which can regulate the secretion of GH,stimulate the appetite,adjust the energy metabolism,and improve the cardiovascular function.Children with congenital heart disease (CHD) usually suffered from malnutrition and growth retardation,which were connected with insufficiency of energy intake,poor absorption,and increase of nutritional requirement,anatomy and the changes of hemodynamics.Many infants with CHD gradually present ventricular dysfunction and growth restriction in their early childhood,which consequently lead to severe complications.Ghrelin is valuable in the early detection of potential CHD,plays an important role in improving the state of malnutrition and protecting the cardiac function,and provides an important reference to explore the new drug therapy for CHD.This review summarized the effects and significances of ghrelin on growth and cardiac function in infants with CHD.

Objective The purposes of the present study are to investigate the effects and mechanisms of Bu Shen Ning Xin Yi Jing Pill on premature ovarian failure (POF) through a clinical study. Methods Sixty women with POF were administered and randomized into the treatment group and the control group. Every group had thirty cases. Patients in the treatment and control groups were requested to have the Bu Shen Ning Xin Yi Jing Pill and the Liu Wei Di Huang Pill, respectively. Six-month duration was considered as a therapeutic course. The serum levels of follicle stimulating hormone (FSH) and estradiol (E2) were determined by the chemistry luminescence method. Differences between the treatment and control groups were investigated.Results Improved menstruation were revealed in both groups and the treatment group showed significant improvement than the control group (P<0.01). In the treatment group, serum FSH levels sharply dropped after treatment, while the serum E2 levels significantly elevated compared with those of prior-treatment (P<0.01) . The total effective rate in the treatment group was 70% (21 cases), whereas 40% in the control group (12 cases). The difference had statistical significance (P<0.01) .Conclusion The BuShen NingXin YiJing Pill is effective in the treatment of premature ovarian failure via significant improvements in the clinical symptoms and balance of the endocrine hormones.

Four methods for evaluating the academic impact of scholars were described according to their literature information, including subjective evaluation method, bibliometric method, social network analysis and comprehen-sive evaluation method .Their development trend was studied by analyzing their characteristics and applications .

Four synthetic peptides,CP10,CP9,CP33c and CP100,with the sequences corresponding to the capsid protein and nonstructural protein of hepatitis C virus(HCV)respectively,were employed to establish ELISA to detect anti-HCV.Serum HCV RNA was identified with reverse transcriptive polymerase chain reaction(RT - PCR)for comparison.Out of 320 sera collected from patients with viral hepatitis,patients with other diseases and blood donors,49 were positive for antibodies against a single or several peptides.Among the positive rates of antibodies against a single peptide,anti-CP10 rate(9.69%)was the highest and anti-CP33c(3.44%)the lowest.The positive rate for anti-HCV antibodies was significantly higher when 4 peptides were combinedly used than when only one peptide(P

Hypertension in elderly patients is a common and frequent disease which could cause stroke, heart failure and renal dysfunction. The pathogenesis of hypertension in the elderly is different from that in the young and middle-aged people, and its clinical characteristics including frequent isolated systolic hypertension, variable blood pressure, high pulse pressure, multiple complications, serious target organ damage, and so on. High prevalence, disability and mortality rates and low awareness, treatment and control rates are prominent phenomena in the elderly patients with hypertension, bringing many difficulties for clinical treatment. Presently, drug therapies for elderly patients mainly include Western medicine (WM), traditional Chinese medicine (TCM), and integrated traditional Chinese and Western medicine. WM therapy has superiority in lowering blood pressure intensively with clear mechanism, but has more adverse reactions. The effects of TCM therapy are multilinked, and TCM is good at reducing side effects and improving symptoms, but TCM preparation falls behind and its hypotensive effects are weak and slow. Furthermore, more multicenter, randomized, controlled and double-blinded clinical trials with large sample are needed to identify the effects of TCM therapy. Based on combination of TCM syndrome differentiation and WM disease differentiation, treatment programs of integrated traditional Chinese and Western medicine would take advantages of both TCM and WM treatment modalities, and are worthy of being studied in the future.

Objective: To detect the correlation of lymphatic vessel density (LVD) with a high mobility group box-1 Protein (HMGB1) and tumor-associated macrophages (TAMs) in cervical carcinoma and the effect on prognosis. Methods:Immunohistochem-istry was applied to detect HMGB1, CD68, and D2-40 expressions in cervical squamous cell carcinoma in 93 cases. t test,χ2 test, Spear-man rank correlation analysis, Kaplan-Meier method, and Cox regression were performed to analyze the expression levels, correlation, and prognosis. Results: HMGB1 protein, CD68, and D2-40 were highly expressed in CSCC. As HMGB1 and TAM expressions in-creased, lymphatic vessel density increased. As HMGB1 and TAM expressions decreased, lymphatic vessel density decreased. Positive correlations were also found between the HMGB1 protein, TAM content, and LVD. In the group with low HMGB1 and TAM expres-sions, the survival time of the group with a high LVD expression was significantly lower than that of the group with a low LVD expres-sion. A multivariate Cox regression model showed that HMGB1 and lymph node metastasis were independent prognostic factors. TAMs and LVD were not independent prognostic factors. Conclusion:HMGB1 proteins and TAMs were highly expressed in CSCC. Patients who exhibit increased HMGB1 expression or increased TAM count consequently show enhanced LVD expressions, increased lymph node metastasis, and poor prognosis.

Objective To explore the effect of prenatal inflammation on rat offspring's blood pressure and behavioral performance.Methods Twenty-four time-mated Sprague-Dawley(SD)rats were randomly divided into three groups,i.e.control,zymosan and LPS.On their gestation days 8,10,12,the pregnant rats received i.p.injections of sterile saline,0.79 mg/kg zymosan or 0.79 mg/kg LPS respectively.When the offspring were sixty weeks old,eight pups were chosen randomly for the later study.Their serum TNF-? level was detected by ELISA using tail blood,arterial blood pressure was measured by tail-cuff method,and their behavioral performance was detected in Morris water maze test.At the end of the experiment,the brains were rapidly taken from the sacrificed offspring,cut into sections and stained with H&E method to investigate the structural changes of hippocampus.Results Compared with control group,all offspring from the inflammation groups showed increased serum level of TNF-?,increased systemic arterial blood pressure,long latency in finding the safe platform and decreased number of neurons in CA1 region of hippocampus.Conclusion Prenatal exposure to LPS or zymosan results in hypertension and disturbance of learning and memory.

Objective:The present research aimed to explore the involvement of ERK signaling pathway in apoptosis of hepatic carcinoma induced by decitabine combined sodium valproate.Methods: HepG2 cell line was incubated with decitabine combined sodium valproate and the inhibition rate was detected by MTT method.The apoptotic related protein and ERK signal pathway proteins were assayed by Real-time PCR or Western blot.Results: The HepG2 cell line was inhibited greatly by decitabine combined sodium valproate medication with an increased expression of Caspase3,Caspase9 and Bax (P<0.05).The expression of Ras,Raf,MEK and ERK1/2 was increased dramatically after incubation with decitabine combined sodium valproate when compared with control group ( P<0.05).Conclusion:Decitabine combined sodium valproate exert a significant inhibition on the proliferation of HepG2 cell line by nor-malizing the abnormal MEK/ERK signaling pathway.

The author has analysed the current employment situation for clinical medicine graduates,the reasons for the difficulty of employment and solutions,so that the basis and the reference can be provided for us to carry out guiding work of clinical medicine graduates.