Objective:To observe the effect of thyroid hormones on 11?-hydroxysteroid dehydrogenase type 1(11?-HSD1) in newborn rats' hippocampus. Methods: Hypothyroid model was induced with propylthiouracil(PTU, 50 mg/d) in SD rats in the last week of pregnancy and was confirmed by measuring the levels of thyroid hormones and thyrotropin(TSH) in the newborn rats' blood. Western blot was used to determine the expression of 11?-HSD1 in hippocampus in newborn rats. Effects of triiodothyronine(T 3) and synthetic glucocorticoid dexamethasone(Dex) on expression of 11?-HSD1 in primarily- cultured hippocampus were studied with thin layer chromatography(TLC) and Western blot. Results: Thyroid hormone levels decreased and TSH level increased in the newborn rats' blood. The expression of 11?-HSD1 decreased by 41.6% in the hippocampus of newborn rats born to the mothers treated with PTU. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) up-regulated the activity of 11?-HSD1 by 29.4%,45.6%,60.9% and 39.8%,respectively. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) increased the expression of 11?-HSD1 protein by 26.0%,43.2%,64.9% and 41.1%,respectively.Synergistic effect of T 3 and DEX was observed in terms of regulating 11?-HSD1: the expression of 11?-HSD1 increased by 123.3% in the hippocampal neurons exposed to both Dex(10 -7 mol/L) and T 3 (10 -7 mol/L) and the activity increased by 114.1%. Conclusion:Thyroid hormone can up-regulate the expression of 11?-HSD1 in the hippocampus during brain maturation either by itself or synergistically with glucocorticoid, thereby enhancing the action of glucocorticoids on the brain.

Objective To explore the high-risk patients with benign prostatic hypeiplasia using bipolar transurethral resection of prostate for the treatment of dual-loop electrosurgical excision of the safety .effectiveness.Methods To retrospectively analyze of 56 cases with high-risk BPH patients with United States bicyclol CIRCON ion bipolar TURP TURP mirror line.Results Operation time was 50～100 minutes,an average of 70 minutes.1 case of mild postoperative incontinence,1PSS score from(28.2 ±1.5)to(7.0 ±1.0).voiding patency,fewer complications.Conclusion 1.Lavage using normal saline to avoid TURS syndrome,operative time can be extended accordingly.2.The current through the body,suitable for patients with special diseases.3.Reducing the incidence of capsularperforation.4.Less bleeding and reduce the occurrence of postoperative complications.5.TURP plasma removal of prostate tissue without charring,suitable for histopathological examination,to avoid the TURP and TUVP undetected pathological examination,increased prostate cancer detection rate.

The chance of craniocerebral injury in children is high.Severe craniocerebral injury is still the difficult point in current clinical treatment.Children severe head trauma is different from adults.It is characteristic of severe primary injury,rapid progressing secondary injury,confusing vital signs,less contrecoup injury,high incidence of epilepsy,cerebral ischemia in familiar,and good prognosis.This article focused on 2 hot issues in recent therapeutic strategy of severe craniocerebral injury in children——intracranial pressure monitoring and hypothermia therapy.The former is simple,convenient,safe and practical,is worth popularizing widely.

A L-arginine-producing mutant UN100-12(SG~(r),AE~(r)) was derived from Corynebacterium glutamicum ATCC138761 by combination treatment with ultraviolet(UV) and N-methyl-N-nitro-N-nitrosoguanidine(NTG). It could accumulate 16.6g/L L-arginine in the medium containing glucose as carbon source and ammonium sulfate as nitrogen source, when it was culrured at 30℃ for 4 days. And also this mutant has good stability of descendiblity of L-arginine.

Estrogen receptor(ER),an important transcription factor belonging to the nuclear receptor superfamily,comprises two subtypes ER?and ER?.Estrogen receptor is expressed in prostate cancer and ladder cancer and has a compacted relationship with them.In this review,we summarized the structure,distri- bution,function of different estrogen receptor subtypes and progress in study on relationship between different estrogen receptor subtypes and prostate or bladder cancer.

A possible ?-Galactosidase gene(pwtsA) was discovered from soil metagenomic DNA of Taishan Mountain.PwtsA gene was inserted into the expression vector pET30a and transferred into E.coli BL21(DE3).Recombinant protein PWTSA was expressed as a soluble form at high level through IPTG induction,with a molecular mass of 57 kD analyzed by SDS-PAGE.PWTSA can produce o-nitrophenol from o-nitrophenol-?-D-galactopyranoside(ONPG),and its specific activity was determined as 13.6 U/mg.The enzymatic studies demonstrated that the recombinant protein PWTSA was a thermostable ?-Galactosidase,its optimum temperature and pH were 85?C～95?C and 6.5 respectively.In standard assays,the Km for ONPG was 0.83 mmol/L.

Dental Impression Materials ; Disinfection

Objective To study the character of transformation of H.pylori into coccoid form under oxidative stress, and the changes in the biological charasteristics after transformation. Methods The coccoid form of H.pylori was studied with bacteriological, enymological, and genetical methods to identify its differences from the original form. Animal experiment was conducted to determine its implantation capacity. Results Morphological transformation from spiral to coccoid form occurred with the prolongation of culture under oxygen stress. The metabolic activity was reduced gradually, finally maintained at a lower level. The activities of urease, alkaline phosphatase and acid phosphatase were decreased markedly. The mRNA levels of main virulence factors were declined. Both mRNA and activities of enzyme of Sod B and Kat retained high levels. A part of the coccoid forms showed reversion to helical form, and colonization in mice stomach could be found after being inoculated to mice. Conclusions The metabolism and virulence of H.pylori were all lowered under oxidative stress, but a part of the coccoid form may be viable. Sod B and Kat may play an important role in the oxygen metabolism of H.pylori.

Objective This study assesses the influence of rizatriptan on calcitonin gene-related peptide (CGRP),proenkephalin (PENK) and cholecystokinin (CCK) mRNA expressions in the trigeminal ganglia of a rat migraine model and investigates the possible mechanisms by which triptans treat migraine.Methods A total of 24 rats were randomly divided into four groups:normal control group (A),migraine model group(B),rizatriptan control group (C) and rizatriptan treatment group(D).Groups C and D were intragastrically perfused with rizatriptan,1 mg/kg per day.After 7 days,nitroglycerin was subcutaneously injected into the buttocks of the groups B and D to induce migraine.Two hours after nitroglycerin injection,the trigeminal ganglia was isolated.CGRP,PENK and CCK mRNA expressions in the trigeminal ganglia were determined using SYBR Green Ⅰ real-time quantitative PCR.Results The copy number of CGRP mRNA (× 107) in 200 ng total RNA of each group was 0.05 ±0.01,1.30 ±0.52,0.23 ±0.12,0.43 ±0.33 ; The copy number of PENK mRNA (× 103) in 200 ng total RNA of each group was 3.30 ± 1.65,0.34 ±0.14,3.91 ± 2.44,0.71 ± 0.13.The copy number of CGRP mRNA in the trigeminal ganglia of group B was significantly higher than that of group A (q =7.854,P ＜ 0.05) ; CGRP mRNA expressions were significantly lower in the trigeminal ganglia of rats in group D compared with group B (q =5.458,P ＜0.05).Compared with group A,PENK mRNA expressions in the trigeminal ganglia of rats were significantly lower in group B (q =4.478,P ＜ 0.05).PENK mRNA expressions were significantly higher in trigeminal ganglia of rats in group C compared with group D (q =4.838,P ＜ 0.05).CCK mRNA expression in trigeminal ganglia of rats was similar among groups.Conelusions Rizatriptan can decrease the expressions of CGRP in the trigeminal ganglia of the migraine rats and exhibits neurogenic inflammation triggered by CGRP.PENK expressions decrease in the trigeminal ganglia of the migraine rats,weaken the analgesic effects of enkephalin.

The pathologic changes of cerebral tissues in hypothyroidism and the molecular mechanism of brain development influenced by lower thyroid hormone levels are reviewed.