Aim To investigate the effects of com-bined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7 . Methods MCF-7 cells were treated with SAHA and/or TRAIL. The inhibitory rates were detected by real-time cell prolifer-ation assays. Morphology changes of MCF-7 cells were observed through time-lapse live cell imaging acquisi-tion. Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was sig-nificantly enhanced in combination with TRAIL. The results of time-lapse live cell imaging acquisition dem-onstrated that, with treatment of SAHA and TRAIL, the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone. Con-clusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.

Anniversaries and Special Events ; Genomics ; Humans ; Internationality ; Mammography ; utilization ; Neoplasms ; diagnosis ; drug therapy ; genetics ; prevention & control

Objective To investigate the risk factors of feeding intolerance in preterm infants and provide reference for the prevention and intervention.Methods Two hundred and Seventy preterm infants were included in the study.The clinical data were collected to analyze the risk factors.Results There were 108 cases showed feeding intolerance.Gestational age,weight,1 minute Apgar,fetal distress,application of aminophylline,stomach bleeding,application of ventilator,prenatal use of GCs,abnormal placenta,perinatal infection were related factors of feeding intolerance in preterm infants.Multivariate Logistic regression analysis showed that application of aminophylline,stomach bleeding,application of ventilator were the independent risk factors of premature infants with feeding intolerance,gestational age was protective factors of feeding intolerance.Conclusion Premature infants with feeding intolerance caused by a variety of factors,and the reasonable measures of prevention and intervention were needed.

OBJECTIVE: To study the preparation of Jingluoning film-coated tablets. METHODS: The preparation of Jingluoning film-coated tablets was optimize by single factor method with granule characteristics, distribution of particle size, angle of repose, surface finish, rigidity and disintegration time limit as index. RESULTS: The optimal preparation method was as follows: the weight proportion of dry extraction to starch, dextrin and silica powder was 16.7 ∶ 16 ∶ 3 ∶ 1; the wetting agent was 90% alcohol; qualified tablets were prepared through wet granulation, tablet compressing and film coating. CONCLUSION: The preparation method is simple and practical. Prepared tablets were characterized with light weight, fast disintegration, low moisture content and good appearance and their quality is in line with the tablet general requirements stated in Chinese Pharmacopoeia (2005 edition).

OBJECTIVE:To study the pharmacokinetics of domestic nimodipin capsule in 12 healthy volunteers.METHO_DS:An oral dose of 60mg nimodipin capsule was given to healthy volunteers,and plasma concentrations of nimodipin were determined by HPLC.The data were fitted by 3p97 program.RESULTS:The results showed that the plasma concentration-time curves profile conformed to a two compartment open model.The T1/2?,Tmax,Cmax,AUC(0～6) of this product was(2.53?1.18)h,(0.42?0.14)h,(75.41?27.65)ng/ml and (155.85?49.54)ng/(h?ml),respectively.CONCLUSION:Retention time of this preparation in healthy volunteers is short with Tmax of about 0.4h,which provides useful information for clinical practice.

Humans ; Sleep Apnea, Obstructive ; etiology

Audiology ; trends ; China ; Humans ; Speech-Language Pathology ; Voice

History, 20th Century ; History, 21st Century ; Otolaryngology ; history ; Otorhinolaryngologic Surgical Procedures

China ; Endoscopy ; trends ; Humans ; Otorhinolaryngologic Surgical Procedures ; trends