From January, 1960 to April. 1983, 292 cases of primary liver cancer were treated by liver resection. Of them, 21 cases underwent re-exploratory operation for relapsed reason and 14 tumours were removed with a resectability rate of 66.7%.In this series recurred tumours in 3 patients were resected by a two-stage operation with hepatic artery ligation or embolizaton as the first step.Following-up results: 7 out of 14 resected cases survivors, 2 survived above one year, 4 more than half a year and 1 less than half a year; in another 7 cases died post-reoperativelly, 1 above one year, 4 more than half a year, 1 less than half a year.As for AFP detection 4 patients were negative and in 1 patient the AFP titre decreased to 100 ug/ml on the 33rd post-reoperative day. From the above data the authors believe that re-operation may be considered as a most effective method if it is performed at a proper time and radiotherapy, hepatic artery ligation or embolization are of great value in treating recurred cases. For the improvment of the surgical results of primary liver cancer,a preliminary scheme for investion and management procedure for such patients is suggested by the authors as well.

Objective To study the clinical features and differential diagnosis of hairy-cell leukemia variant (HCL-V).Methods A case with HCL-V was reported and the literatures were reviewed.Results The patient had splenomegaly for twenty years and a history of recurrent pulmonary infection.His blood routine test showed a high white blood cell count and abnormal high proportion of lymphocytes.Peripheral smear and bone marrow smear both showed significantly higher proportion of lymphocytes,part of which had soma jagged,prominent nucleoli and villous/hairy cytoplasmic projections.His hairy leukemic cells expressed CD1 1c,CD19,CD20,CD22,and had variable expression of FMC-7,CD103 and lambda,but not CD5,CD23 and CD25.Transmission electron microscope showed many monocytes with villous exist in peripheral blood.Conclusions HCL-V is a rare and an indolent form of a small,mature,B-cell leukemia,based on the clinical,peripheral smear,bone marrow smear,flow cytometric analysis and transmission electron microscopy features,a diagnosis of HCL-V is confirmed.The differential diagnosis should always include splenic marginal zone B-cell lymphoma and HCL-C,because they have different clinical and biological features.

Hypoxia is a common physiological and pathological stimulus in many human diseases .The cellular oxygen sensors and the following activation of multiple cellular signal transduction pathways involved in hypoxia responses can regulate cell survival as well as respiration , blood circulation , metabolism and so forth .The cell response to hypoxia has a complex diversity .Hypoxia-induc-ible factor ( HIF) pathway in an oxygen dependent manner plays a central role during the hypoxia response .The HIF-independent path-ways are equally important under hypoxic conditions which can maintain the oxygen balance and metabolism balance .

Objective To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana. Method The medium composition and cultivation conditions were optimized by orthogonal and single factor experiments. Re-sults The experiments showed that the conditions of initial pH 3,fermentation temperature 30℃,volume of liquid 100 ml(250 ml Erlenmeyer flask),and 3D-xylitol 0.5 g/L were optimum,and the molluscicidal activity of the fermentation filtrate reached 95%. After three hatches of cultivation,the predicted values were verified by validation experiments. Conclusion Endophyte LL3026 from Buddleia lindleyana has a good molluscicidal activity after the optimization.

Thirty-seven essential hypertensive patients were treated with nifedipine and captopril. The effects of both drugs on high blood pressure and inteacellular cations were reported.A significant reduction in leucocyte Na concentration compared to the baseline were noted after therapy (P

ObjectiveTo study the effect of of oral powered microdedrider adenoidectomy with nasal endoscope in children with secretory otitis media. Methods74 children with secrctory otitis media treated with adenoidectomy with nasal endoscope randomly divided into group A 37 cases and group B 37 cases,the group A were treated with trans-oral adenoidectomy under endoscope, the group B were treated with trans-nasal adenoidectomy under endoscope,then the total effective rate,operation time, blood loss,incidence of complications and serum IgE, NO, IL-6,TNF-αand IL-2 of the two groups before and after the treatment were compared. ResultsThe cure, effective, and ineffective of group A were 34 cases ,3cases and 0 cases ,the total effective rate was 100.0％, the cure, effective,and ineffective of group B were 26 cases,7 cases and 4 cases,the total effective rate was 89.2％ ,the total effective rate of group A was significantly higher than that of group B( x2 =6. 869 ,P ＜0.05 ) ,the operation time of group A was( 8.6± 1.6) min, blood loss was( 12.3 ± 4. 1 ) ml, incidence of complications was 2.7％ ( 1 case postoperative hemorrhage), operation time of group B was ( 14.2 ± 2.1 ) min, blood loss was( 17.8 ± 3.5 ) ml, incidence of complications was 10.8％ (2 cases nasal adhesion and 2 cases postoperative hemorrhage) ,there were significant differences between the two groups ( t =5. 967,6.553, x2 =7. 142, all P ＜ 0.05 ), serum IgE, NO, IL-6,TNF-αand IL-2of the group A after the treatment were all lower than those of group B, ( t =5. 557,6. 348,5. 968,6. 976,5. 884, all P ＜ 0. 05 ).ConclusionThe effect of oral powered microdedrider adenoidectomy with nasal endoscope in children with secretory otitis media better,and it was worthy of popularization and application.

ObjectiveTo study the relationship between human papilloma virus(HPV) infections,p53 polymorphisms and esophageal cancer.MethodsA case-control study of 204 esophageal cancer cases (esophageal cancer group) and 102 healthy control cases (control group) biopsies were conducted to extract DNA,detected the HPV by PCR method and analyzed p53 polymorphisms by PCR-RFLP method,then analyzed the relationship between them and esophageal cancer incidence rate.ResultsThe positive rate of HPV16 in esophageal cancer group[59.3％(121/204)] was higher than that in control group [11.8％ ( 12/102 ) ] (P ＜ 0.05 ).And the frequency of Arg/Arg genttype of p53 in esophageal cancer group [ 51.5％ (105/204) ] was higher than that in control group [ 19.6％ (20/102) ] (P ＜ 0.05 ).The frequency of Arg/Arg genotypo in HPV16 positive patients [66.9％(81/121 )] was higher than that in HPV16 negative patients [ 28.9％ (24/83)] (P ＜ 0.05).ConclusionsHigh risk HPV phys an important role in development of esophageal cancer,p53 Arg/Arg genotype is one of high risk genetic factors for HPV-associated esophageal cancer.

Objective To establish the plane strain model of brain tissues on mid-sagittal plane and discuss the relation between the brain tissue deformation and brain injury. Methods By referring to the computerized tomography pictures of the human body and using plane strain and finite element method (FEM), we applied plane strain hypothesis to establish a model for calculation of the brain tissue deformation caused by rotational inertia loading during vehicle crash accident. Results At the 12th ms, the shear strain field adjacent to parietal lobe reached 0.148 and the maximum positive shear stress located at white material of the cerebellum was up to 8 088.0 Pa. At the 28th ms, the maximum shear stress located at the cerebellum reached 900.9 Pa. Great shear strain existed in the cerebrospinal fluid region all the time and shear stress with large absolute value presented on the boundary of white matter and gray matter. Conclusions Under rotational inertia loading, the shear strain (stress) in brain tissues can lead to diffuse axonal injury. The uneven strain (stress) may cause serious avulsion damage in brain tissues.

Objective To understand the prevalence of major non-communicable chronic diseases among the adults in the airport district of Beijing.Methods With the same proportion and multi-stage stratified cluster sampling,the prevalent rates of primary existing chronic diseases were investigated in 650 residents aged over 18 years and lived in Beijing for more than 6 months.Each participant was invited to receive a set of standardized questionnaire,physical examinations and laboratory tests.Results The prevalent rates of hypertension,diabetes,dyslipidemia,acute myocardial infarction and the stroke were 26.0%,5.3%,39.9%,1.3% and 2.0%,respectively.The awareness rates for hypertension and diabetes were 54.4% and 3.6%,respectively.Conclusion The prevalent rates of chronic non-communicable diseases of the adults in this area were in higher levels.The relevant department should pay more attention to it.