1.Studies on the Morphological Changes of Neurons in Dorsal Root Ganglion and Spinal Dorsal Horn of the Rat with an Experimental Peripheral Neuropathy.
Won Taek LEE ; Min Gyun SON ; Gyung Seung JUNG ; Hong Suk LEE ; Gyung Ah PARK
Korean Journal of Anatomy 1997;30(5):495-510
Animal models for human chronic pain syndromes were developed and widely used for pain research. One of thsese neuropathic pain model by Kim and Chung[1992] has many advantages for operation and pain elicitation. We have examined the c-fos protein, substance P, CGRP immunoreactivity in dorsal root ganglia and dorsal horn in this neuropathic model. About 50 Sprague-Dawley rats were used for this study. L5 and L6 spinal nerve were ligated tightly to produce neuropathic pain model. After 2, 4, 8, 16, 24 hours and 1 week of surgery, rats were anesthesized and sacrificed by perfusion through the left ventricle with saline followed by 0.1M phosphate buffer[pH 7.4] containing 3% paraformaldehyde, 3% glutaraldehyde, and 0.1% picric acid. After confirmation of the roots transected by the surgery, the L5 and L6 dorsal root ganglion and spinal cord were removed and processed for immunohistochemistry. All tissue sections were immunohistochemically stained for substance P, CGRP and c-fos by using the peroxidase-antiperoxidase[PAP] method. Count the number of immunostained substance P and CGRP dorsal root ganglion cells and c-fos immunoreactive dorsal horn cells and analyzed statistically with Mann-Whitney U test. The results are as follows. 1. The number of c-fos protein immunoreactive neurons in the superficial layer of dorsal horn were increased markedly at 2 hours after operation, gradually decreased to normal level 1 week after operation. 2. The number of c-fos protein immunoreactive neurons in the deep layer of dorsal horn were gradually increased to the peak 24 hours after operation, decreased to normal level 1 week after operation. 3. The number of substance P and CGRP immunoreactive L5 and L6 dorsal root ganglion neurons were decreased markedly at 1 week after pain model operation. In conclusion, after neuropathic pain model operation, c-fos protein were immediately expressed in the superficial layer of spinal dorsal horn, thereafter c-fos protein in the deep layer of spinal dorsal horn were expressed. CGRP and substance P immunoreactive neurons were decreased markedly 1 week after neuropathic pain model operation.
Animals
;
Chronic Pain
;
Ganglia, Spinal*
;
Glutaral
;
Heart Ventricles
;
Horns*
;
Humans
;
Immunohistochemistry
;
Models, Animal
;
Neuralgia
;
Neurons*
;
Perfusion
;
Peripheral Nervous System Diseases*
;
Posterior Horn Cells
;
Rats*
;
Rats, Sprague-Dawley
;
Spinal Cord
;
Spinal Nerve Roots*
;
Spinal Nerves
;
Substance P
2.Bronchogenic Cyst Causing Mitral Regurgitation.
Jong Pil SONG ; Seung Hyuck JUNG ; Gyung Hun KANG ; Byung Yeol KIM ; Gyung Min KANG
The Korean Journal of Thoracic and Cardiovascular Surgery 1999;32(1):66-69
Bronchogenic cyst is an uncommon congenital lesion which is derived from the primitive foregut. Most bronchogenic cyst may develope at the tracheal bifurcation, both main bronchi, the lung parenchymeand the mediastinum. A 40-year old male was evaluated for dyspnea and chest tightness. Computed tomography revealed a well dermarcated, 7.2 x 7.9 cm sized, homogeneous mass compressing the left atrium. 2D-echo showed grade III mitral regurgitation. We completely removed the cystic mass and then confirmed the bronchogenic cyst in the pathological diagnosis. During the follow up period, the patient progressed well without any symptoms and showed grade I mitral regurgitation on the 2D-Echo. Therefore, we report a case of the bronchogenic cyst causing grade III mitral regurgitation.
Adult
;
Bronchi
;
Bronchogenic Cyst*
;
Diagnosis
;
Dyspnea
;
Follow-Up Studies
;
Heart Atria
;
Humans
;
Lung
;
Male
;
Mediastinum
;
Mitral Valve Insufficiency*
;
Thorax
3.A Case of Pseudomyxoma Peritonei with Primary Borderline Mucinous Tumors of the Ovary and Appendix.
Yeon Jung KIM ; Hee Ok KIM ; Gyung Ae JUNG ; Min Jin LEE ; Woon Sub HAN ; Seung Chul KIM
Korean Journal of Obstetrics and Gynecology 2000;43(11):2067-2071
Pseudomyxoma peritonei is a poorly understood condition characterized by mucinous ascites and multifocal peritoneal mucinous tumors. Pseudomyxoma peritonei is most commonly associated with mucinous tumors of the ovary and appendix. When two lesions are morphologically similar, it is difficult to determine whether one reflects a metastasis or whether the tumors are independent. Many studies concluded that most cases in which both the appendix and ovary were involved were primary appendiceal tumor with secondary involvement of the ovary, but we have experienced one case of pseudomyxoma peritonei originated from independent primary borderline mucinous tumors of the ovary and appendix. So we report this case with the brief review of literatures
Appendix*
;
Ascites
;
Female
;
Mucins*
;
Neoplasm Metastasis
;
Ovary*
;
Pseudomyxoma Peritonei*
4.Surgical Treatment of Esophageal Stricture in Barrett's Esophagus: A Case Report.
Yong Chul SHIN ; Seung Hyuck JUNG ; Gyung Min KANG ; Gyung Hun KIM ; Chul Hwan KIM ; Kyung Hoon KANG ; Yong HUR ; Byung Yul KIM ; Jung Ho LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 1997;30(4):457-461
A casts of Barrett's esophagus complicated with stricture is reported. A 82 years old male was undergone distal esophagectomy and esophagogastrostomy on suspicion of malignancy. The microscopic examination of specimen taken from the lesion revealed the "tall columnar cells" which were indicative of Barrett's esophagus, hence the diagnosis. Barrett's esophagus is a rare disease in Korea. Because of the malignant potential of the disease, surgery involving esophagectomy is often required.
Aged, 80 and over
;
Barrett Esophagus*
;
Constriction, Pathologic
;
Diagnosis
;
Esophageal Stenosis*
;
Esophagectomy
;
Humans
;
Korea
;
Male
;
Rare Diseases
5.A Case of Mixed Form of Type III Congenital Cystic Adenomatoid Malformation and Extralobar Pulmonary Sequestration of The Lung.
Chang Ho JUNG ; Jin Bum SONG ; Hye Eun LEE ; Seung Hwan JEON ; Gyung Yong SEO ; Jae Sik SHIM ; Mi Young KIM
Korean Journal of Obstetrics and Gynecology 2000;43(8):1464-1468
No abstract available.
Bronchopulmonary Sequestration*
;
Cystic Adenomatoid Malformation of Lung, Congenital*
;
Lung*
6.Expression of Cell Surface Marker on Human Bone Marrow Derived Stromal Cells during Chondrogenic Differentiation.
Sang Gyung KIM ; Jung Yoon CHOE ; Chae Gi KIM ; Seung Hie CHUNG ; Im Hee SHIN ; Hun Suk SUH
The Journal of the Korean Rheumatism Association 2005;12(1):30-37
OBJECTIVE: Multipotent bone marrow stromal cells have the ability to differentiate toward a variety of connective tissue lineages including cartilage. The future use of adult mesenchymal stem cells (MSCs) for human therapies depends on the establishment of preclinical studies. Therefore, in this preclinical study we demonstrated the expression of MSC surface markers CD29, CD105, and CD44 on human bone marrow derived stromal cells during chondrogenic differentiation. METHODS: Adult human bone marrow was collected from the iliac crest of 7 donors following informed consent. Mononuclear cells were isolated, incubated in monolayers, and embedded in alginate beads for three-dimensional cultures. Cellualr viability was assessed by MTT assay. Flow cytometry of alginate bead cultures was performed on days 0, 7, 14, 21, and 28 using monoclonal antibody against surface molecules, CD105, CD29, CD44, CD34 and CD45. Total contents of collagen and glycosaminoglycan (GAG) of the alginate beads was measured. SPSS 11.0 was used for data analysis. RESULTS: After 7 days of culture, 89% of the cells expressed the human integrin beta 1 antibody, CD29. The CD29-positive cells remained elevated at 83% on days 28. However, while only 18% expressed the type II TGF-beta receptor endoglin, CD105 on day 7, the CD105-positive cells increased abruptly 65% on day 14 remaining elevated up to day 28. The expression of CD44 was maximal in the first passage cell (63%). High concentration of TGF-beta 3 (10 ng/mL) was more favorable for sustaining cell viability than a low concentration (0.5 ng/mL)(n=4, p= 0.002, day 21). The total contents of collagen and GAG in the MSC-alginate beads increased during the three-dimensional culture (n=4, p=0.02, p=0.006) suggesting its differentiation into a chondrogenic lineage. CONCLUSION: CD29 was expressed earlier than CD105 during chondrogenic differentiation of human bone marrow MSC. CD44 expression was highest in the first passage cells and gradually decreased afterwards.
Adult
;
Bone Marrow*
;
Cartilage
;
Cell Survival
;
Collagen
;
Connective Tissue
;
Flow Cytometry
;
Humans*
;
Informed Consent
;
Mesenchymal Stromal Cells
;
Receptors, Transforming Growth Factor beta
;
Statistics as Topic
;
Stromal Cells*
;
Tissue Donors
;
Transforming Growth Factor beta
7.Development of Screening Test for Adolescent Mental Health and Problem Behavior.
Seung Ah JUNG ; Dong Hyun AHN ; Sun Yuo CHUNG ; Youn Gyung JEONG ; Yun Yung KIM
Journal of Korean Neuropsychiatric Association 2008;47(2):168-176
OBJECTIVES: This study was conducted to develop the screening questionnaire (Adolescent Mental Health and Problem Behavior Questionnaire, AMPQ) for detecting mental health problems and various problem behaviors in adolescents. METHODS: First, six main domains of questionnaire were defined and preliminary sub-items were collected through literature study, consultation data from field mental health experts, and preliminary survey study. Next, with these 52 preliminary items, we conducted nationwide survey on 1,267 middle and high school students of five districts of Korea. RESULTS: Through reliability test and factor analysis 34 items were finally filtered out. The Cronbach's alpha of these 34 items was .88. And correlation with K-YSR and K-CBCL were .34 (p<.01) and .68 (p<.01). CONCLUSION: AMPQ, which is comprised of relatively short list of items and at the same time covers overall mental health and problem behaviors in adolescents can be used by non-professionals as a screening questionnaire in school settings or mental health centers.
Adolescent
;
Humans
;
Mass Screening
;
Mental Health
;
Surveys and Questionnaires
8.Association of Carotid Intraplaque Hemorrhage and Territorial Acute Infarction in Patients with Acute Neurological Symptoms Using Carotid Magnetization-Prepared Rapid Acquisition with Gradient-Echo.
Jung Soo PARK ; Hyo Sung KWAK ; Jong Myong LEE ; Eun Jeong KOH ; Gyung Ho CHUNG ; Seung Bae HWANG
Journal of Korean Neurosurgical Society 2015;57(2):94-99
OBJECTIVE: The purpose of our study was to assess prevalence of carotid intraplaque hemorrhage (IPH) and associations between territorial acute infarction and IPH on magnetization-prepared rapid acquisition with gradient-echo (MPRAGE) in patients with acute neurologic symptoms. METHODS: 83 patients with suspected acute neurologic symptoms were evaluated with both brain diffusion weighted imaging (DWI) and carotid MPRAGE sequences. Carotid plaque with high signal intensity on MPRAGE of >200% that of adjacent muscle was categorized as IPH. We analyzed the prevalence of IPH and its correlation with territorial acute infarction. RESULTS: Of 166 arteries, 39 had a carotid artery plaque. Of these arteries, 26 had carotid artery stenosis less than 50%. In all carotid arteries, MR-depicted IPH was found in 7.2% (12/166). High-signal intensity on DWI was found in 17.5% (29/166). Combined lesion with ipsilateral high-signal intensity on DWI and IPH on carotid MPRAGE sequence was found in 6 lesions (6/166, 3.6%). Of patients with carotid artery plaque, MR-predicted IPH was found in 30.8% (12/39) and match lesions with high-signal intensity on DWI and MPRAGE was found in 15.4% (6/39). MR-predicted IPH was significantly higher prevalence in high-grade stenosis group (p=0.010). Relative risk between carotid MPRAGE-positive signal and ipsilateral high-signal intensity on DWI in arteries with carotid artery plaques was 6.8 (p=0.010). CONCLUSION: Carotid MPRAGE-positive signal in patients was associated with an increased risk of territorial acute infarction as detected objectively by brain DWI. The relative risk of stroke was increased in high-grade stenosis categories.
Arteries
;
Atherosclerosis
;
Brain
;
Carotid Arteries
;
Carotid Stenosis
;
Constriction, Pathologic
;
Diffusion
;
Hemorrhage*
;
Humans
;
Infarction*
;
Magnetic Resonance Imaging
;
Neurologic Manifestations
;
Prevalence
;
Stroke
9.A case of diffuse alveolar hemorrhage following administration of artecoll.
Gyung Nam KIM ; Jung In KIM ; Beom Ju LEE ; Kyoung Taek HAN ; Seung Il LEE ; Sung Ho YOON
Korean Journal of Medicine 2004;67(2):190-193
Diffuse alveolar hemorrhage (DAH) is a relatively uncommon disorder that most often occurs in patients with systemic autoimmune or idiopathic disease. DAH may result from coagulation disorders, inhaled toxins, or infection. The principal histopathologic features of pulmonary capillaritis include capillary wall necrosis with infiltration by neutrophils, interstitial erythrocytes, and/or hemosiderin, and interalveolar septal capillary occlusion by fibrin thrombi. New inert materials such as polymerized silicones, artecoll is used as injectable aesthetic microimplants. They are still able to stimulate a clinically evident granulomatous reaction. The rate of allergic reaction is very low. We report the case of diffuse alveolar hemorrhage following administration of artecoll.
Capillaries
;
Collagen
;
Erythrocytes
;
Fibrin
;
Hemorrhage*
;
Hemosiderin
;
Humans
;
Hypersensitivity
;
Necrosis
;
Neutrophils
;
Polymers
;
Silicones
10.Effect of Type 16 Human Papillomavirus Positivity in Uterine Cervix and Follicular Fluid of Infertile Women and Sperm of Their Spouses on Outcomes of In Vitro Fertilization and Embryo Transfer.
Suk Hyun KIM ; Eun Gyung KIM ; Seung Yeob KOO ; Byung Chul JEE ; Chang Suk SEO ; Young Min CHOI ; Jung Goo KIM ; Sin Yong MOON ; Jin Yong LEE
Korean Journal of Obstetrics and Gynecology 2000;43(8):1414-1421
No abstract available.
Cervix Uteri*
;
Embryo Transfer*
;
Embryonic Structures*
;
Female
;
Fertilization in Vitro*
;
Follicular Fluid*
;
Humans*
;
Spermatozoa*
;
Spouses*