The uptake of glucose for metabolism and growth is essential to most animal cells and is mediated by glucose-transporter (GLUT) proteins. The aim of this study was to determine which class of glucose transporter molecules was responsible for uptake of glucose in the mouse early embryo and at which stage the corresponding genes were expressed. In addition, co-culture system with vero cell was used to investigate the effect of the system on GLUT expression. Two-cell stage embryos were collected from the superovulated ICR female and divided into 3 groups. As a control, embryos were cultured in 0.4% BSA-T6 medium which includes glucose. For the experimental groups, embryos were cultured in either co-culture system with vero cells or glucose-free 76 medium supplemented with 0.4% BSA and pyruvate as an energy substrate. 2-cell to blastocyst stage embryos in those groups were respectively collected into microtubes (50 embryos/tube). Total RNA was extracted and RT-PCR was performed. The products were analysed after staining ethidium bromide by 2% agarose gel electrophoresis. Blastocysts were collected from each group at 120hr after hCG injection. They were fixed in 2.5% glutaraldehyde, stained with hoechst, and mounted for observation. In control, GLUT1 was expressed from 4-cell to blastocyst. GLUT2 and GLUT3 were expressed in morula and blastocyst. GLUT4 was expressed in all stages. When embryos were cultured in glucose-free medium, no significant difference was shown in the expression of GLUTI1, 2 and 3, compared to control. However GLUT4 was not expressed until morular stage. When embryos were co-cultured with vero cell, there was no significant difference in the expression of GLUT1, 2, 3 and 4 compared to control. To determine cell growth of embryos, the average cell number of blastocyst was counted. The cell number of co-culture (93.8+/-3.1, n=35) is significantly higher than that of control and glucose-free group (76.6 +/- 3.8, n=35 and 68.2+/-4.3, n=30). This study shows that the GLUT genes are expressed differently according to embryo stage. GLUTs were detectable throughout mouse preimplantation development in control and co-culture groups. However, GLUT4 was not detected from 2- to 8-cell stage but detected from morula stage in glucose-free medium, suggested that GLUT genes are expressed autocrinally in the embryo regardless of the presence of glucose as an energy substrate. In addition, co-culture system can increase the cell count of blastocyst but not improve the expression of GLUT. In conclusion, expression of GLUT is dependent on embryo stage in preimplantation embryo development.
Animals ; Blastocyst ; Cell Count ; Coculture Techniques ; Electrophoresis, Agar Gel ; Embryonic Development ; Embryonic Structures* ; Ethidium ; Female ; Glucose Transport Proteins, Facilitative* ; Glucose* ; Glutaral ; Humans ; Metabolism ; Mice* ; Morula ; Pregnancy ; Pyruvic Acid ; RNA ; Vero Cells

In patients with severely resorbed alveolar bone, it is difficult to gain retention in denture. Lack of retention makes denture unstable and lead to trouble in using denture. Suction denture seals the entire denture border with movable mucosa and this sealing mechanism forms negative pressure beneath the denture and produce higher retention and stability to denture. In this case, 4 edentulous patients visited for lack of retention with dentures. Considering their high expectation with retention, suction denture concept was used to fabricate retentive and stable denture. The purpose of this case report is to compare and analyze the considerations of suction denture restorations in edentulous patients.
Denture, Complete ; Dentures ; Humans ; Mucous Membrane ; Rehabilitation ; Suction

PURPOSE: To report a case of Purtscher's retinopathy accompanied by serous retinal detachment in a patient with retinitis pigmentosa (RP) who was referred to us for treatment of post-traumatic visual discomfort. CASE SUMMARY: A 36-year-old man with history of RP was referred to us with the chief complaint of bilateral visual discomfort after chest injury from a traffic accident. His corrected visual acuity was 0.3 and 0.6 in the right and left eyes, respectively. Fundus examination revealed findings characteristic of RP in both eyes, along with a lesion in the right eye, which was suspected to be a serous elevation of the macula, as well as suspected exudates near the optic nerves. Optical coherence tomography indicated serous retinal detachment in the right eye, and fluorescein angiography findings were characteristic of RP. Seven days later, the amount of cotton-wool exudate in the right eye had increased and was more distinct than at the initial examination, and retinal hemorrhage was observed. Based on the medical history and specific fundus findings, the patient was diagnosed with Purtscher's retinopathy. One month later, the serous retinal detachment in the right eye had improved, but the vision loss and total anopsia in the right eye persisted. CONCLUSIONS: The concomitant occurrence of RP and Purtscher's retinopathy is very rare. Moreover, the presence of accompanying serous retinal detachment and delayed onset of typical clinical symptoms are not present in typical Purtscher's retinopathy.
Accidents, Traffic ; Adult ; Exudates and Transudates ; Fluorescein Angiography ; Humans ; Optic Nerve ; Retinal Detachment* ; Retinal Hemorrhage ; Retinaldehyde* ; Retinitis Pigmentosa* ; Retinitis* ; Thoracic Injuries ; Tomography, Optical Coherence ; Visual Acuity

PURPOSE: To evaluate the clinical features of compound nevus. METHODS: A retrospective chart review of the medical records for 32 eyes of 32 patients who were clinically diagnosed as having a compound nevus from February 2011 to February 2017 was performed. RESULTS: The average follow-up period was 21.38 (range, 6–70) months for the 32 patients (9 males and 32 females), and the average age was 21 (range, 7–41) years old. The development or detection of a nevus varied between patients. There were no associated symptoms except for one patient who experienced foreign body sensation. An increase in size was noted in 5 cases (15%). The most common location in the conjunctiva was bulbar in 30 cases (93.8%), and the most common quadrant was temporal in 21 cases (65.6%) followed by nasal conjunctiva in 11 cases (34.4%). The most common locations of anterior margin and posterior margin were on the limbus (56%) and bulbar conjunctiva (92%), respectively. The mean horizontal length was 2.59 ± 1.9 mm and the mean vertical length was 2.62 ± 2.1 mm. All horizontal and vertical lengths were within 5 mm. An elevated nevus was observed in 25 cases (78.1%), and 18 cases (56.3%) had cystic lesions. The color of the nevi were largely brown (26 cases, 81.3%), and 29 cases (90.6%) had feeder vessels. Excisional biopsy and histologic exam were performed in 22 cases (68.8%). The purpose of the treatment was mostly cosmetic (20 cases, 93.8%) or for differential diagnosis with malignant melanoma (2 cases, 6.3%). In the surgery group, no one showed recurrence or any significant complications. CONCLUSIONS: A conjunctival compound nevus is a benign conjunctival mass and can be found in all age groups. Compound nevus can be diagnosed with clinical features, and complete excision with histological exam is an appropriate treatment to differentiate from malignant melanoma.
Biopsy ; Conjunctiva ; Diagnosis, Differential ; Follow-Up Studies ; Foreign Bodies ; Humans ; Male ; Medical Records ; Melanoma ; Nevus* ; Recurrence ; Retrospective Studies ; Sensation

Helicobacter pylori is the most important etiology of peptic ulcer disease. With the gradual eradication of H. pylori and the increase in the use of nonsteroidal anti-inflammatory drugs, the proportion of H. pylori-negative peptic ulcers has been increasing. It is important for false negative results to be ruled out in H. pylori-negative peptic ulcers, and medical treatment for long-term maintenance should be considered because of the high risk of recurrence and related complications associated with the disease.
Helicobacter pylori ; Helicobacter* ; Peptic Ulcer* ; Recurrence

Pure-phase beta-tricalcium phosphate(beta-TCP) proved to be a bone regeneration material, providing the patient with vital bone at the defect site in a reasonable time, making a second surgical procedure for bone harvesting unnecessary. This study compares bone healing and BMP 2/4 expression in cranial defects in rabbits grafted with autogenous bone and beta-TCP. Thirty New Zealand White rabbits was divided into 3 group of 10 animals each. Bilateral calvarial defects were made in the parietal bones of each animal. beta-TCP placed in one defect and the other defects was filled with autogenous bone. The animal were sacrificed at 4, 8 and 12 weeks. Immunohistochemical analysis was used to investigate the expression of BMP 2/4. 1. The new bone formation around autogenous bone from 4 weeks and beta-TCP from 8 weeks. 2. In autogenous bone graft, BMP 2/4 expression was decreased from 4 to 12 weeks. 3. In beta-TCP graft, BMP 4 expression was increased from 8 to 12 weeks. But, BMP 2 was observed from 12 weeks. This study showed that bone healing, regeneration and, BMP 2/4 expression are delayed in grafted beta-TCP than autogenous bone.
Animals ; Bone Regeneration* ; Humans ; Osteogenesis ; Parietal Bone ; Rabbits ; Regeneration ; Transplants

Gastrointestinal subepithelial tumors (GSTs) are usually detected incidentally on endoscopic or radiologic examinations. In conventional endoscopy, a GST usually presents as a protuberant lesion with an intact mucosal surface. As the lesion is located beneath the mucosal layer of the gastrointestinal tract, conventional biopsy typically does not reveal the pathologic diagnosis. First, a GST should be differentiated from an extrinsic compression through the positional change of the patient during conventional endoscopic examination. In cases of GSTs originating from the gastrointestinal wall, endoscopic ultrasonography (EUS) can be beneficial for narrowing the differential diagnosis through delineation of echo findings and by determining the layer of origin. EUS findings can also help determine the management strategies for GSTs by making a differential diagnosis according to malignant potential.
Biopsy ; Diagnosis ; Diagnosis, Differential ; Endoscopy ; Endosonography ; Gastrointestinal Stromal Tumors ; Gastrointestinal Tract ; Humans

BACKGROUND/AIMS: The unavailability of human gastric cell lines representative of the normal gastric epithelial function such as polarized monolayer restricts the application of cell culture system in approaching the field of Helicobacter pylori (H. pylori) infected gastric mucosa models. The present investigation aimed at assessing the usefulness of NCI-N87 cell line as an adequate cellular model to study the pathophysiology of human H. pylori infection. METHODS: For the identification of epithelial phenotypes at low magnification, cells were observed on a phase-contrast microscope and confocal microscope. Transepithelial resistance (TER) was measured on NCI-N87 cells seeded on Transwell(R) to identify monolayer polarity two or three times a week after confluency. The IL-8 level was determined by ELISA at 24 hours after the administration of HP60190 and IL-1alpha on NCI-N87 cells. IL-8 level was compared in both upper and lower well with the control. RESULTS: A monolayer phenotype was observed in NCI-N87 cell lines by using confocal microscope. TER was measured as 400-500 (omega x cm2) at two or three weeks after cell culture. In NCI-N87 cell lines, IL-8 level was significantly increased after 24 hour compared to control, and was prominent in the lower well. CONCLUSIONS: These results suggest that NCI-N87 cell line may be useful in H. pylori infected gastric mucosa model.
Cell Line ; Enzyme-Linked Immunosorbent Assay ; Gastric Mucosa/*microbiology/pathology ; Helicobacter Infections/metabolism/*microbiology/pathology ; *Helicobacter pylori ; Humans ; Interleukin-8/metabolism ; Microscopy, Confocal ; Microscopy, Phase-Contrast ; Phenotype

BACKGROUND/AIMS: Helicobacter pylori (Hp) infection is an important risk factor for gastric carcinogenesis. Although several studies have investigated the effect of Hp eradication on the development of metachronous neoplasm after endoscopic resection of the gastric dysplasia, the evidence is still insufficient to make a clear conclusion. The aims of this study was to evaluate the risk factors for the development of metachronous neoplasm after endoscopic resection of gastric dysplasia and to investigate the effect of Hp eradication. METHODS: Between 2005 and 2011, a total of 887 patients underwent endoscopic resection for gastric dysplasia. Among them, 521 patients who had undergone tests for Hp infection and been followed-up for at least one year were included in the final analyses. Of the 292 Hp-positive patients, 116 patients were successfully eradicated, while 176 failed or did not undergo eradication. RESULTS: During a mean follow-up of 59.1 months (range 12–125 months), metachronous neoplasm had developed in 63 patients (12.1%, dysplasia in 38, carcinoma in 25). In multivariate analyses, age ≥65 (hazard ratio [HR]=2.247, 95% confidence interval [CI] 1.297–3.895), tumor size (HR=1.283, 95% CI 1.038–1.585), synchronous lesion (HR=2.341, 95% CI 1.244–4.405), family history of gastric cancer (HR=3.240, 95% CI 1.776–5.912), and smoking (HR=1.016, 95% CI 1.003–1.029) were risk factors for metachronous neoplasm after endoscopic resection of gastric dysplasia. However, Hp eradication was not associated with metachronous neoplasm (HR=0.641, 95% CI 0.297–1.384). CONCLUSIONS: Hp eradication was not shown to be associated with the development of metachronous cancer after endoscopic resection of gastric dysplasia.
Carcinogenesis ; Follow-Up Studies ; Helicobacter pylori* ; Helicobacter* ; Humans ; Multivariate Analysis ; Neoplasms, Second Primary* ; Precancerous Conditions ; Risk Factors ; Smoke ; Smoking ; Stomach Neoplasms

Disseminated superficial porokeratosis is a keratinization disorder characterized by multiple brown, atrophic patches with sharply demarcated and hypertrophic ridges. Atypical cases of disseminated superficial porokeratosis with acute exacerbation accompanied by severe pruritus have been reported as "eruptive pruritic papular porokeratosis" or "inflammatory disseminated superficial porokeratosis." We report a case of inflammatory disseminated superficial porokeratosis in a 53-year-old man who was successfully treated with oral isotretinoin.
Humans ; Isotretinoin ; Keratins ; Middle Aged ; Porokeratosis ; Pruritus